Rw2G016330

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001043
Physical Location & Seq
Forward (+)
19218554 .. 19219394
841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G016330.1

Sequence Viewer

Length: 765 bp
ATGGAGGATCGTGACATCAACAAAGACATGGCTGAAATCCAAAGGATGCTTCAGCAACTTGCGAGTTCCGATGGGGAGAGAGACGTTACCATCTATCATCAACGTGTTGATCGCATCGAAACTTTCTACCAAGATGGTGACGGTTCTGACGGGGAGATATGCATTGGCCCAATTCATAATTGTGCTCCGCTGTGTGAGTCAAATGAAGAGGTACATAGTACTTTCAACTATTCCGGTTGTAAGTCCACTATCTTTAATTTTTCATTTGTTGAGGAAGTATTTTTAGAAAAAGAAAAAAATAATTTCATGAGTTTTGATATACCACCGAAGTATGATTGTTATGAAGATTCTGAAGTTTGTGATGGTGGTGTGGAGGACCATAGGAAAAATGAATTTGTTCCTAGAAATTGTGATGATGAAGAAGACAATAAAGCCGCAATCAAGTCAATATTGTGGAAAGATATTGAGCATAAATTTGTTGATTTTATTGGAGTTAATGCATTCATTATGCATATTCCCAAAGAATTAGTTAATCTTGTCACTCAGATAAAAGATGGTGAGAAAAATTTTGGGATTGCGAAATTAATGTCGGAGAGAAAATGTATGCCAAATATCTATTTGTTCAACAAAAGATATGAAGCAATGGAAGACTTTGTCAGGATTTCTTCAATCAATGACTCAAATTCGAGGGCGAATTCTTTCCAACCAGGGGAGTCTGATGCAGGAGCATATGTGGCAATATCAAATGATGCATTTGATATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

254

Amino Acids

29.15

Weight (kDa)

4.55

Isoelectric Point (pI)

53.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 188, 435
AclWI GGATC 1 cut(s) 15
AcsI RAATTY 5 cut(s) 392, 473, 565, 682, 694
AcuI CTGAAG 2 cut(s) 35, 372
AfaI GTAC 2 cut(s) 213, 220
AfiI CCNNNNNNNGG 1 cut(s) 709
AflIII ACRYGT 1 cut(s) 103
AgsI TTSAA 3 cut(s) 226, 625, 669
AjnI CCWGG 1 cut(s) 706
AjuI GAANNNNNNNTTGG 2 cut(s) 33, 65
Alw21I GWGCWC 1 cut(s) 187
Alw26I GTCTC 1 cut(s) 75
AlwI GGATC 1 cut(s) 15
AoxI GGCC 1 cut(s) 166
ApoI RAATTY 5 cut(s) 392, 473, 565, 682, 694
ArsI GACNNNNNNTTYG 2 cut(s) 572, 604
AseI ATTAAT 1 cut(s) 584
Asp700I GAANNNNTTC 2 cut(s) 396, 698
AspS9I GGNCC 2 cut(s) 167, 376
AsuHPI GGTGA 2 cut(s) 149, 569
AvaII GGWCC 1 cut(s) 376
BbsI GAAGAC 2 cut(s) 429, 654
Bbv12I GWGCWC 1 cut(s) 187
BccI CCATC 5 cut(s) 65, 98, 128, 356, 548
BciT130I CCWGG 1 cut(s) 708
BcoDI GTCTC 1 cut(s) 75
BfaI CTAG 1 cut(s) 402
BisI GCNGC 1 cut(s) 435
BlsI GCNGC 1 cut(s) 436
BmcAI AGTACT 1 cut(s) 220
Bme1390I CCNGG 1 cut(s) 708
Bme18I GGWCC 1 cut(s) 376
BmgT120I GGNCC 2 cut(s) 167, 376
BmrFI CCNGG 1 cut(s) 708
BmsI GCATC 4 cut(s) 36, 123, 709, 739
BpiI GAAGAC 2 cut(s) 429, 654
BsaJI CCNNGG 1 cut(s) 707
BsaWI WCCGGW 1 cut(s) 233
BsaXI ACNNNNNCTCC 2 cut(s) 717, 747
Bsc4I CCNNNNNNNGG 1 cut(s) 709
Bse3DI GCAATG 1 cut(s) 648
BseBI CCWGG 1 cut(s) 708
BseDI CCNNGG 1 cut(s) 707
BseGI GGATG 1 cut(s) 51
BseLI CCNNNNNNNGG 1 cut(s) 709
BseMI GCAATG 1 cut(s) 648
BseMII CTCAG 1 cut(s) 557
BshFI GGCC 1 cut(s) 168
BsiHKAI GWGCWC 1 cut(s) 187
BsiSI CCGG 1 cut(s) 234
BslI CCNNNNNNNGG 1 cut(s) 709
BsmAI GTCTC 1 cut(s) 75
BsmBI CGTCTC 1 cut(s) 75
BsmI GAATGC 1 cut(s) 500
BsnI GGCC 1 cut(s) 168
Bsp1286I GDGCHC 1 cut(s) 187
Bsp143I GATC 2 cut(s) 7, 109
BspACI CCGC 2 cut(s) 188, 435
BspANI GGCC 1 cut(s) 168
BspCNI CTCAG 1 cut(s) 556
BspHI TCATGA 1 cut(s) 306
BspPI GGATC 1 cut(s) 15
BsrDI GCAATG 1 cut(s) 648
BssECI CCNNGG 1 cut(s) 707
BssMI GATC 2 cut(s) 7, 109
Bst2UI CCWGG 1 cut(s) 708
Bst4CI ACNGT 1 cut(s) 143
Bst6I CTCTTC 1 cut(s) 201
BstDEI CTNAG 1 cut(s) 543
BstF5I GGATG 1 cut(s) 51
BstKTI GATC 2 cut(s) 10, 112
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 2 cut(s) 7, 109
BstMWI GCNNNNNNNGC 1 cut(s) 734
BstNI CCWGG 1 cut(s) 708
BstSCI CCNGG 1 cut(s) 706
BstV2I GAAGAC 2 cut(s) 429, 654
BsuRI GGCC 1 cut(s) 168
BtsCI GGATG 1 cut(s) 51
CciI TCATGA 1 cut(s) 306
Cfr13I GGNCC 2 cut(s) 167, 376
Csp6I GTAC 2 cut(s) 212, 219
CviAII CATG 2 cut(s) 28, 307
CviJI RGCY 3 cut(s) 32, 168, 434
CviKI_1 RGCY 3 cut(s) 32, 168, 434
CviQI GTAC 2 cut(s) 212, 219
DdeI CTNAG 1 cut(s) 543
DpnI GATC 2 cut(s) 9, 111
DpnII GATC 2 cut(s) 7, 109
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 376
Eco57I CTGAAG 2 cut(s) 35, 372
EcoRI GAATTC 1 cut(s) 694
EcoRII CCWGG 1 cut(s) 706
EcoT22I ATGCAT 4 cut(s) 164, 502, 513, 754
Esp3I CGTCTC 1 cut(s) 75
FaeI CATG 2 cut(s) 31, 310
FatI CATG 2 cut(s) 27, 306
FauNDI CATATG 1 cut(s) 730
Fnu4HI GCNGC 1 cut(s) 435
FokI GGATG 1 cut(s) 58
Fsp4HI GCNGC 1 cut(s) 435
FspBI CTAG 1 cut(s) 402
GluI GCNGC 1 cut(s) 435
HaeIII GGCC 1 cut(s) 168
HapII CCGG 1 cut(s) 234
Hin1II CATG 2 cut(s) 31, 310
HinfI GANTC 4 cut(s) 197, 347, 677, 713
HpaII CCGG 1 cut(s) 234
HphI GGTGA 2 cut(s) 149, 569
Hpy166II GTNNAC 1 cut(s) 246
Hpy188I TCNGA 6 cut(s) 70, 148, 352, 546, 592, 718
Hpy188III TCNNGA 3 cut(s) 11, 307, 658
Hpy8I GTNNAC 1 cut(s) 246
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4IV ACGT 2 cut(s) 84, 103
HpyCH4V TGCA 5 cut(s) 162, 500, 511, 722, 752
HpyF10VI GCNNNNNNNGC 1 cut(s) 734
HpyF3I CTNAG 1 cut(s) 543
HpySE526I ACGT 2 cut(s) 84, 103
Hsp92II CATG 2 cut(s) 31, 310
Kzo9I GATC 2 cut(s) 7, 109
LmnI GCTCC 2 cut(s) 190, 725
LpnPI CCDG 5 cut(s) 247, 643, 693, 708, 720
LweI GCATC 4 cut(s) 36, 123, 709, 739
MaeI CTAG 1 cut(s) 402
MaeII ACGT 2 cut(s) 84, 103
MaeIII GTNAC 4 cut(s) 11, 85, 137, 538
MalI GATC 2 cut(s) 9, 111
MboI GATC 2 cut(s) 7, 109
MboII GAAGA 6 cut(s) 218, 356, 431, 434, 657, 659
MhlI GDGCHC 1 cut(s) 187
MlyI GAGTC 3 cut(s) 206, 671, 722
MmeI TCCRAC 2 cut(s) 570, 727
MnlI CCTC 4 cut(s) 202, 265, 367, 681
Mph1103I ATGCAT 4 cut(s) 164, 502, 513, 754
MroXI GAANNNNTTC 2 cut(s) 396, 698
MseI TTAA 5 cut(s) 255, 495, 531, 584, 763
MslI CAYNNNNRTG 2 cut(s) 102, 180
MspA1I CMGCKG 1 cut(s) 190
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 1 cut(s) 708
Mva1269I GAATGC 1 cut(s) 500
MvaI CCWGG 1 cut(s) 708
MwoI GCNNNNNNNGC 1 cut(s) 734
NdeI CATATG 1 cut(s) 730
NdeII GATC 2 cut(s) 7, 109
NlaIII CATG 2 cut(s) 31, 310
NmuCI GTSAC 3 cut(s) 11, 137, 538
NsiI ATGCAT 4 cut(s) 164, 502, 513, 754
PagI TCATGA 1 cut(s) 306
PctI GAATGC 1 cut(s) 500
PdmI GAANNNNTTC 2 cut(s) 396, 698
PfeI GAWTC 1 cut(s) 347
PflFI GACNNNGTC 1 cut(s) 653
PkrI GCNGC 1 cut(s) 436
PleI GAGTC 3 cut(s) 205, 671, 721
PpsI GAGTC 3 cut(s) 205, 671, 721
PshBI ATTAAT 1 cut(s) 584
Psp6I CCWGG 1 cut(s) 706
PspGI CCWGG 1 cut(s) 706
PspPI GGNCC 2 cut(s) 167, 376
PsyI GACNNNGTC 1 cut(s) 653
RsaI GTAC 2 cut(s) 213, 220
RsaNI GTAC 2 cut(s) 212, 219
RseI CAYNNNNRTG 2 cut(s) 102, 180
SaqAI TTAA 5 cut(s) 255, 495, 531, 584, 763
SatI GCNGC 1 cut(s) 435
Sau3AI GATC 2 cut(s) 7, 109
Sau96I GGNCC 2 cut(s) 167, 376
ScaI AGTACT 1 cut(s) 220
SchI GAGTC 3 cut(s) 206, 671, 722
ScrFI CCNGG 1 cut(s) 708
SduI GDGCHC 1 cut(s) 187
SetI ASST 3 cut(s) 87, 106, 213
SfaNI GCATC 4 cut(s) 36, 123, 709, 739
SinI GGWCC 1 cut(s) 376
SmiMI CAYNNNNRTG 2 cut(s) 102, 180
SsiI CCGC 2 cut(s) 188, 435
SspI AATATT 1 cut(s) 450
SspMI CTAG 1 cut(s) 402
StyD4I CCNGG 1 cut(s) 706
TaaI ACNGT 1 cut(s) 143
TaiI ACGT 2 cut(s) 87, 106
TaqI TCGA 2 cut(s) 117, 686
TatI WGTACW 1 cut(s) 218
TauI GCSGC 1 cut(s) 437
TfiI GAWTC 1 cut(s) 347
Tru1I TTAA 5 cut(s) 255, 495, 531, 584, 763
Tru9I TTAA 5 cut(s) 255, 495, 531, 584, 763
TseFI GTSAC 3 cut(s) 11, 137, 538
Tsp45I GTSAC 3 cut(s) 11, 137, 538
TspDTI ATGAA 9 cut(s) 164, 219, 252, 295, 357, 405, 432, 493, 651
Tth111I GACNNNGTC 1 cut(s) 653
VpaK11BI GGWCC 1 cut(s) 376
VspI ATTAAT 1 cut(s) 584
XapI RAATTY 5 cut(s) 392, 473, 565, 682, 694
XmnI GAANNNNTTC 2 cut(s) 396, 698
XspI CTAG 1 cut(s) 402
ZrmI AGTACT 1 cut(s) 220
Zsp2I ATGCAT 4 cut(s) 164, 502, 513, 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.