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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 173 |
| AfaI | GTAC | 2 cut(s) | 210, 385 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 245 |
| AgsI | TTSAA | 3 cut(s) | 89, 221, 369 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 311, 343 |
| AluBI | AGCT | 2 cut(s) | 139, 203 |
| AluI | AGCT | 2 cut(s) | 139, 203 |
| Alw21I | GWGCWC | 1 cut(s) | 38 |
| AlwI | GGATC | 1 cut(s) | 173 |
| AsuHPI | GGTGA | 2 cut(s) | 281, 323 |
| AsuII | TTCGAA | 1 cut(s) | 12 |
| AsuNHI | GCTAGC | 1 cut(s) | 139 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 378, 411 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 192, 224 |
| BbsI | GAAGAC | 1 cut(s) | 234 |
| Bbv12I | GWGCWC | 1 cut(s) | 38 |
| BccI | CCATC | 2 cut(s) | 62, 220 |
| BclI | TGATCA | 2 cut(s) | 55, 418 |
| BfaI | CTAG | 2 cut(s) | 140, 351 |
| BmcAI | AGTACT | 1 cut(s) | 385 |
| BmsI | GCATC | 1 cut(s) | 304 |
| BmtI | GCTAGC | 1 cut(s) | 143 |
| BpiI | GAAGAC | 1 cut(s) | 234 |
| Bpu14I | TTCGAA | 1 cut(s) | 12 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 245 |
| BseGI | GGATG | 2 cut(s) | 10, 382 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 245 |
| BsiHKAI | GWGCWC | 1 cut(s) | 38 |
| BsiSI | CCGG | 1 cut(s) | 362 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 245 |
| Bsp119I | TTCGAA | 1 cut(s) | 12 |
| Bsp1286I | GDGCHC | 1 cut(s) | 38 |
| Bsp143I | GATC | 3 cut(s) | 55, 178, 418 |
| BspOI | GCTAGC | 1 cut(s) | 143 |
| BspPI | GGATC | 1 cut(s) | 173 |
| BspT104I | TTCGAA | 1 cut(s) | 12 |
| BssMI | GATC | 3 cut(s) | 55, 178, 418 |
| Bst4CI | ACNGT | 1 cut(s) | 208 |
| BstBI | TTCGAA | 1 cut(s) | 12 |
| BstC8I | GCNNGC | 1 cut(s) | 141 |
| BstDEI | CTNAG | 1 cut(s) | 431 |
| BstF5I | GGATG | 2 cut(s) | 10, 382 |
| BstKTI | GATC | 3 cut(s) | 58, 181, 421 |
| BstMBI | GATC | 3 cut(s) | 55, 178, 418 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 42 |
| BstNSI | RCATGY | 1 cut(s) | 169 |
| BstV2I | GAAGAC | 1 cut(s) | 234 |
| BtsCI | GGATG | 2 cut(s) | 10, 382 |
| Cac8I | GCNNGC | 1 cut(s) | 141 |
| Csp6I | GTAC | 2 cut(s) | 209, 384 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 204, 239 |
| CviAII | CATG | 3 cut(s) | 107, 166, 356 |
| CviJI | RGCY | 4 cut(s) | 139, 203, 361, 430 |
| CviKI_1 | RGCY | 4 cut(s) | 139, 203, 361, 430 |
| CviQI | GTAC | 2 cut(s) | 209, 384 |
| DdeI | CTNAG | 1 cut(s) | 431 |
| DpnI | GATC | 3 cut(s) | 57, 180, 420 |
| DpnII | GATC | 3 cut(s) | 55, 178, 418 |
| FaeI | CATG | 3 cut(s) | 110, 169, 359 |
| FatI | CATG | 3 cut(s) | 106, 165, 355 |
| FbaI | TGATCA | 2 cut(s) | 55, 418 |
| FokI | GGATG | 2 cut(s) | 17, 389 |
| FspBI | CTAG | 2 cut(s) | 140, 351 |
| HapII | CCGG | 1 cut(s) | 362 |
| Hin1II | CATG | 3 cut(s) | 110, 169, 359 |
| HinfI | GANTC | 1 cut(s) | 365 |
| HpaII | CCGG | 1 cut(s) | 362 |
| HphI | GGTGA | 2 cut(s) | 281, 323 |
| Hpy188I | TCNGA | 1 cut(s) | 60 |
| Hpy188III | TCNNGA | 1 cut(s) | 422 |
| HpyAV | CCTTC | 1 cut(s) | 83 |
| HpyCH4III | ACNGT | 1 cut(s) | 208 |
| HpyCH4IV | ACGT | 1 cut(s) | 321 |
| HpyCH4V | TGCA | 3 cut(s) | 19, 163, 169 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 42 |
| HpyF3I | CTNAG | 1 cut(s) | 431 |
| HpySE526I | ACGT | 1 cut(s) | 321 |
| Hsp92II | CATG | 3 cut(s) | 110, 169, 359 |
| Ksp22I | TGATCA | 2 cut(s) | 55, 418 |
| Kzo9I | GATC | 3 cut(s) | 55, 178, 418 |
| LpnPI | CCDG | 2 cut(s) | 375, 407 |
| LweI | GCATC | 1 cut(s) | 304 |
| MaeI | CTAG | 2 cut(s) | 140, 351 |
| MaeII | ACGT | 1 cut(s) | 321 |
| MalI | GATC | 3 cut(s) | 57, 180, 420 |
| MboI | GATC | 3 cut(s) | 55, 178, 418 |
| MboII | GAAGA | 1 cut(s) | 239 |
| MfeI | CAATTG | 2 cut(s) | 46, 216 |
| MhlI | GDGCHC | 1 cut(s) | 38 |
| MluCI | AATT | 7 cut(s) | 46, 73, 174, 183, 216, 258, 414 |
| MnlI | CCTC | 2 cut(s) | 76, 267 |
| MslI | CAYNNNNRTG | 1 cut(s) | 309 |
| MspI | CCGG | 1 cut(s) | 362 |
| MunI | CAATTG | 2 cut(s) | 46, 216 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 42 |
| NdeII | GATC | 3 cut(s) | 55, 178, 418 |
| NheI | GCTAGC | 1 cut(s) | 139 |
| NlaIII | CATG | 3 cut(s) | 110, 169, 359 |
| NspI | RCATGY | 1 cut(s) | 169 |
| NspV | TTCGAA | 1 cut(s) | 12 |
| PfeI | GAWTC | 1 cut(s) | 365 |
| RsaI | GTAC | 2 cut(s) | 210, 385 |
| RsaNI | GTAC | 2 cut(s) | 209, 384 |
| RseI | CAYNNNNRTG | 1 cut(s) | 309 |
| Sau3AI | GATC | 3 cut(s) | 55, 178, 418 |
| ScaI | AGTACT | 1 cut(s) | 385 |
| SduI | GDGCHC | 1 cut(s) | 38 |
| SetI | ASST | 7 cut(s) | 87, 94, 141, 205, 234, 250, 324 |
| SfaNI | GCATC | 1 cut(s) | 304 |
| SfuI | TTCGAA | 1 cut(s) | 12 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 309 |
| Sse9I | AATT | 7 cut(s) | 46, 73, 174, 183, 216, 258, 414 |
| SspI | AATATT | 2 cut(s) | 97, 154 |
| SspMI | CTAG | 2 cut(s) | 140, 351 |
| TaaI | ACNGT | 1 cut(s) | 208 |
| TaiI | ACGT | 1 cut(s) | 324 |
| TaqI | TCGA | 2 cut(s) | 12, 195 |
| TasI | AATT | 7 cut(s) | 46, 73, 174, 183, 216, 258, 414 |
| TatI | WGTACW | 1 cut(s) | 383 |
| TfiI | GAWTC | 1 cut(s) | 365 |
| TspDTI | ATGAA | 3 cut(s) | 117, 213, 302 |
| XceI | RCATGY | 1 cut(s) | 169 |
| XspI | CTAG | 2 cut(s) | 140, 351 |
| ZrmI | AGTACT | 1 cut(s) | 385 |