Rroxscaffold_1G00053230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001043
Physical Location & Seq
Reverse (-)
74063605 .. 74066133
2529 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00053230.1

Sequence Viewer

Length: 945 bp
ATGGAGGGTCATGGCATCAACAACGACATGGCTGAAATTAAAAGGATGCTTCAGCAACTTGCGTACGTATCGATCGCATTGAAACTCGACGACGGGGTCGTAAGAGTTCCGACGGGGAGAGAGACGTTACCACTTATCATCAATGTGTCGATCGCATCGAAACTCGACGACGAGATAGTAAGAGTTCCGACGGGGAGAGAAACGTTACCACTTATCATCAACGTGTCGATCGCATCGAAACTCGACGACGAGATCGTAAGAGTTCCGACGGGGAGAGAAACGTTACCACCTATCATCAATGTGTCAATCGCTTCGAAGTTTCACACCAAAGCGGTGAGAGTATTGAGGAGGAACAATACATTGAACTCTCTCATCGCCGTGCTTCTTTGTGTGAGCCGTATATACGGGATGAAGATTGCGATGAAAATTGCTTGGAGATTGAAGCGGTTGACCTGCCCATGCAAAGGAATTATCTCCCGAGTGCAACAAAATATAGTTAAAGCCATGCCAGTCCATCTTGTGAAAACATCATCAGCAATTGTAAGTCATGAAATTAATTCATGCATGGGTGATATCTTTGAATTGGATGATCACGTGGGTAATTCAGATCCAAAGCTCAAACTAGATGTGGATGTTGGCATTGTTTTAGGATCTCAAGAAATAGAACATAGTATGTGTGGGTCAATACGTGAGCATCCTAATATAATTGTTGGTGTTGATCTTAAGAAGTCATTTGTCTCCAACAAACCGGCAAGGAGTGTTGAATCTAAAAGTAAAGATGCATCCCTCAAATTTCATGATCCATTACAAGAAGATGCAACTCATCAATTTATTGATTTCCATGATCCATTACAAGAAGATGCAACTCATCAATTTATTGATTTCATTGGGGAAGAGCAACAGCCACTGTCAGATTCTAAAATCTGCATTGCCAGATTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

314

Amino Acids

34.94

Weight (kDa)

6.83

Isoelectric Point (pI)

35.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 95
Acc36I ACCTGC 1 cut(s) 461
AciI CCGC 2 cut(s) 332, 445
AclI AACGTT 2 cut(s) 203, 281
AclWI GGATC 4 cut(s) 602, 658, 794, 839
AcsI RAATTY 1 cut(s) 791
AcuI CTGAAG 1 cut(s) 35
AcvI CACGTG 1 cut(s) 595
AfaI GTAC 1 cut(s) 65
AfiI CCNNNNNNNGG 1 cut(s) 464
AflII CTTAAG 1 cut(s) 722
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 5 cut(s) 82, 364, 442, 581, 764
AluBI AGCT 1 cut(s) 616
AluI AGCT 1 cut(s) 616
Alw26I GTCTC 2 cut(s) 116, 742
AlwI GGATC 4 cut(s) 602, 658, 794, 839
AlwNI CAGNNNCTG 1 cut(s) 907
Ama87I CYCGRG 1 cut(s) 477
ApoI RAATTY 1 cut(s) 791
AseI ATTAAT 1 cut(s) 555
AsuHPI GGTGA 2 cut(s) 346, 581
AsuII TTCGAA 1 cut(s) 314
AvaI CYCGRG 1 cut(s) 477
BbrPI CACGTG 1 cut(s) 595
BccI CCATC 1 cut(s) 522
BceAI ACGGC 2 cut(s) 362, 381
BclI TGATCA 1 cut(s) 589
BcoDI GTCTC 2 cut(s) 116, 742
BfaI CTAG 2 cut(s) 623, 943
BfrI CTTAAG 1 cut(s) 722
BfuAI ACCTGC 1 cut(s) 461
BmeT110I CYCGRG 1 cut(s) 477
BmsI GCATC 9 cut(s) 24, 36, 164, 242, 703, 769, 791, 805, 850
Bpu14I TTCGAA 1 cut(s) 314
BpuEI CTTGAG 1 cut(s) 639
Bsa29I ATCGAT 1 cut(s) 71
BsaAI YACGTR 3 cut(s) 67, 595, 689
Bsc4I CCNNNNNNNGG 1 cut(s) 464
Bse118I RCCGGY 1 cut(s) 748
Bse1I ACTGG 1 cut(s) 509
Bse3DI GCAATG 1 cut(s) 927
BseCI ATCGAT 1 cut(s) 71
BseGI GGATG 6 cut(s) 51, 414, 592, 637, 694, 782
BseLI CCNNNNNNNGG 1 cut(s) 464
BseMI GCAATG 1 cut(s) 927
BseNI ACTGG 1 cut(s) 509
BseRI GAGGAG 1 cut(s) 361
Bsh1285I CGRYCG 3 cut(s) 75, 153, 231
BshVI ATCGAT 1 cut(s) 71
BsiEI CGRYCG 3 cut(s) 75, 153, 231
BsiHKCI CYCGRG 1 cut(s) 477
BsiSI CCGG 1 cut(s) 749
BsiWI CGTACG 1 cut(s) 63
BslI CCNNNNNNNGG 1 cut(s) 464
BsmAI GTCTC 2 cut(s) 116, 742
BsmBI CGTCTC 1 cut(s) 116
BsoBI CYCGRG 1 cut(s) 477
Bsp119I TTCGAA 1 cut(s) 314
BspACI CCGC 2 cut(s) 332, 445
BspDI ATCGAT 1 cut(s) 71
BspHI TCATGA 2 cut(s) 547, 796
BspMI ACCTGC 1 cut(s) 461
BspPI GGATC 4 cut(s) 602, 658, 794, 839
BspQI GCTCTTC 1 cut(s) 888
BspT104I TTCGAA 1 cut(s) 314
BspTI CTTAAG 1 cut(s) 722
BsrDI GCAATG 1 cut(s) 927
BsrFI RCCGGY 1 cut(s) 748
BsrI ACTGG 1 cut(s) 509
BssAI RCCGGY 1 cut(s) 748
Bst4CI ACNGT 1 cut(s) 909
Bst6I CTCTTC 1 cut(s) 888
BstAFI CTTAAG 1 cut(s) 722
BstBAI YACGTR 3 cut(s) 67, 595, 689
BstBI TTCGAA 1 cut(s) 314
BstF5I GGATG 6 cut(s) 51, 414, 592, 637, 694, 782
BstMAI GTCTC 2 cut(s) 116, 742
BstMCI CGRYCG 3 cut(s) 75, 153, 231
BstSNI TACGTA 1 cut(s) 67
BstX2I RGATCY 2 cut(s) 607, 650
BstYI RGATCY 2 cut(s) 607, 650
Bsu15I ATCGAT 1 cut(s) 71
BsuTUI ATCGAT 1 cut(s) 71
BtgZI GCGATG 2 cut(s) 358, 434
BtsCI GGATG 6 cut(s) 51, 414, 592, 637, 694, 782
BtsIMutI CAGTG 1 cut(s) 905
BveI ACCTGC 1 cut(s) 461
CaiI CAGNNNCTG 1 cut(s) 907
CciI TCATGA 2 cut(s) 547, 796
Cfr10I RCCGGY 1 cut(s) 748
ClaI ATCGAT 1 cut(s) 71
Csp6I GTAC 1 cut(s) 64
CviAII CATG 9 cut(s) 11, 28, 459, 505, 548, 561, 565, 797, 842
CviJI RGCY 5 cut(s) 32, 396, 503, 616, 904
CviKI_1 RGCY 5 cut(s) 32, 396, 503, 616, 904
CviQI GTAC 1 cut(s) 64
DrdI GACNNNNNNGTC 1 cut(s) 95
DseDI GACNNNNNNGTC 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 888
EarI CTCTTC 1 cut(s) 888
Eco105I TACGTA 1 cut(s) 67
Eco32I GATATC 1 cut(s) 574
Eco57I CTGAAG 1 cut(s) 35
Eco72I CACGTG 1 cut(s) 595
Eco88I CYCGRG 1 cut(s) 477
EcoRV GATATC 1 cut(s) 574
EcoT22I ATGCAT 2 cut(s) 566, 784
Esp3I CGTCTC 1 cut(s) 116
FaeI CATG 9 cut(s) 14, 31, 462, 508, 551, 564, 568, 800, 845
FatI CATG 9 cut(s) 10, 27, 458, 504, 547, 560, 564, 796, 841
FbaI TGATCA 1 cut(s) 589
FokI GGATG 6 cut(s) 58, 421, 599, 644, 681, 769
FspBI CTAG 2 cut(s) 623, 943
HapII CCGG 1 cut(s) 749
Hin1II CATG 9 cut(s) 14, 31, 462, 508, 551, 564, 568, 800, 845
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HinfI GANTC 3 cut(s) 764, 914, 936
HpaII CCGG 1 cut(s) 749
HphI GGTGA 2 cut(s) 346, 581
Hpy166II GTNNAC 1 cut(s) 450
Hpy188I TCNGA 5 cut(s) 111, 189, 267, 607, 913
Hpy188III TCNNGA 4 cut(s) 477, 548, 656, 797
Hpy8I GTNNAC 1 cut(s) 450
Hpy99I CGWCG 9 cut(s) 92, 95, 115, 170, 173, 193, 248, 251, 271
HpyCH4III ACNGT 1 cut(s) 909
HpyCH4IV ACGT 7 cut(s) 66, 125, 203, 222, 281, 594, 688
HpyCH4V TGCA 7 cut(s) 462, 484, 564, 782, 818, 863, 927
HpySE526I ACGT 7 cut(s) 66, 125, 203, 222, 281, 594, 688
Hsp92II CATG 9 cut(s) 14, 31, 462, 508, 551, 564, 568, 800, 845
Ksp22I TGATCA 1 cut(s) 589
LguI GCTCTTC 1 cut(s) 888
LpnPI CCDG 3 cut(s) 466, 522, 762
LweI GCATC 9 cut(s) 24, 36, 164, 242, 703, 769, 791, 805, 850
MaeI CTAG 2 cut(s) 623, 943
MaeII ACGT 7 cut(s) 66, 125, 203, 222, 281, 594, 688
MaeIII GTNAC 3 cut(s) 126, 204, 282
MboII GAAGA 5 cut(s) 424, 824, 869, 905, 931
MfeI CAATTG 1 cut(s) 537
MflI RGATCY 2 cut(s) 607, 650
MmeI TCCRAC 4 cut(s) 134, 212, 290, 765
MnlI CCTC 3 cut(s) 339, 342, 797
Mph1103I ATGCAT 2 cut(s) 566, 784
MseI TTAA 4 cut(s) 39, 498, 555, 723
MslI CAYNNNNRTG 4 cut(s) 143, 221, 299, 377
MspCI CTTAAG 1 cut(s) 722
MspI CCGG 1 cut(s) 749
MunI CAATTG 1 cut(s) 537
NlaIII CATG 9 cut(s) 14, 31, 462, 508, 551, 564, 568, 800, 845
NsiI ATGCAT 2 cut(s) 566, 784
NspV TTCGAA 1 cut(s) 314
PagI TCATGA 2 cut(s) 547, 796
PciSI GCTCTTC 1 cut(s) 888
PcsI WCGNNNNNNNCGW 4 cut(s) 96, 155, 233, 252
PfeI GAWTC 3 cut(s) 764, 914, 936
Pfl23II CGTACG 1 cut(s) 63
PflFI GACNNNGTC 1 cut(s) 95
Ple19I CGATCG 3 cut(s) 75, 153, 231
PmaCI CACGTG 1 cut(s) 595
PmlI CACGTG 1 cut(s) 595
Ppu21I YACGTR 3 cut(s) 67, 595, 689
PshBI ATTAAT 1 cut(s) 555
Psp1406I AACGTT 2 cut(s) 203, 281
PspCI CACGTG 1 cut(s) 595
PspLI CGTACG 1 cut(s) 63
PstNI CAGNNNCTG 1 cut(s) 907
PsuI RGATCY 2 cut(s) 607, 650
PsyI GACNNNGTC 1 cut(s) 95
PvuI CGATCG 3 cut(s) 75, 153, 231
RsaI GTAC 1 cut(s) 65
RsaNI GTAC 1 cut(s) 64
RseI CAYNNNNRTG 4 cut(s) 143, 221, 299, 377
SapI GCTCTTC 1 cut(s) 888
SaqAI TTAA 4 cut(s) 39, 498, 555, 723
SfaNI GCATC 9 cut(s) 24, 36, 164, 242, 703, 769, 791, 805, 850
SfuI TTCGAA 1 cut(s) 314
SmiMI CAYNNNNRTG 4 cut(s) 143, 221, 299, 377
SmlI CTYRAG 2 cut(s) 654, 722
SmoI CTYRAG 2 cut(s) 654, 722
SnaBI TACGTA 1 cut(s) 67
SsiI CCGC 2 cut(s) 332, 445
SspMI CTAG 2 cut(s) 623, 943
TaaI ACNGT 1 cut(s) 909
TaiI ACGT 7 cut(s) 69, 128, 206, 225, 284, 597, 691
TaqI TCGA 9 cut(s) 71, 87, 149, 158, 165, 227, 236, 243, 314
TfiI GAWTC 3 cut(s) 764, 914, 936
Tru1I TTAA 4 cut(s) 39, 498, 555, 723
Tru9I TTAA 4 cut(s) 39, 498, 555, 723
TscAI CASTG 1 cut(s) 912
TspDTI ATGAA 6 cut(s) 425, 437, 549, 564, 785, 874
TspRI CASTG 1 cut(s) 912
Tth111I GACNNNGTC 1 cut(s) 95
Vha464I CTTAAG 1 cut(s) 722
VspI ATTAAT 1 cut(s) 555
XapI RAATTY 1 cut(s) 791
XspI CTAG 2 cut(s) 623, 943
Zsp2I ATGCAT 2 cut(s) 566, 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.