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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 364 |
| AclWI | GGATC | 2 cut(s) | 127, 335 |
| AcsI | RAATTY | 1 cut(s) | 136 |
| AcuI | CTGAAG | 2 cut(s) | 162, 351 |
| AfaI | GTAC | 3 cut(s) | 14, 132, 398 |
| AgsI | TTSAA | 4 cut(s) | 56, 263, 352, 372 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 344, 376 |
| AluBI | AGCT | 4 cut(s) | 41, 59, 335, 375 |
| AluI | AGCT | 4 cut(s) | 41, 59, 335, 375 |
| AlwI | GGATC | 2 cut(s) | 127, 335 |
| AoxI | GGCC | 1 cut(s) | 96 |
| ApoI | RAATTY | 1 cut(s) | 136 |
| AsuHPI | GGTGA | 1 cut(s) | 167 |
| BanI | GGYRCC | 1 cut(s) | 364 |
| BccI | CCATC | 1 cut(s) | 146 |
| BfaI | CTAG | 1 cut(s) | 219 |
| BfmI | CTRYAG | 1 cut(s) | 123 |
| BmiI | GGNNCC | 1 cut(s) | 366 |
| BseMII | CTCAG | 1 cut(s) | 81 |
| BshFI | GGCC | 1 cut(s) | 98 |
| BshNI | GGYRCC | 1 cut(s) | 364 |
| BsnI | GGCC | 1 cut(s) | 98 |
| Bsp143I | GATC | 2 cut(s) | 119, 340 |
| BspANI | GGCC | 1 cut(s) | 98 |
| BspCNI | CTCAG | 1 cut(s) | 82 |
| BspLI | GGNNCC | 1 cut(s) | 366 |
| BspMAI | CTGCAG | 1 cut(s) | 127 |
| BspPI | GGATC | 2 cut(s) | 127, 335 |
| BspT107I | GGYRCC | 1 cut(s) | 364 |
| BssMI | GATC | 2 cut(s) | 119, 340 |
| Bst6I | CTCTTC | 1 cut(s) | 330 |
| BstDEI | CTNAG | 1 cut(s) | 90 |
| BstKTI | GATC | 2 cut(s) | 122, 343 |
| BstMBI | GATC | 2 cut(s) | 119, 340 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 95 |
| BstSFI | CTRYAG | 1 cut(s) | 123 |
| BstX2I | RGATCY | 1 cut(s) | 119 |
| BstYI | RGATCY | 1 cut(s) | 119 |
| BsuRI | GGCC | 1 cut(s) | 98 |
| BtsI | GCAGTG | 1 cut(s) | 306 |
| BtsIMutI | CAGTG | 1 cut(s) | 306 |
| Csp6I | GTAC | 3 cut(s) | 13, 131, 397 |
| CviAII | CATG | 1 cut(s) | 65 |
| CviJI | RGCY | 6 cut(s) | 41, 59, 89, 98, 335, 375 |
| CviKI_1 | RGCY | 6 cut(s) | 41, 59, 89, 98, 335, 375 |
| CviQI | GTAC | 3 cut(s) | 13, 131, 397 |
| DdeI | CTNAG | 1 cut(s) | 90 |
| DpnI | GATC | 2 cut(s) | 121, 342 |
| DpnII | GATC | 2 cut(s) | 119, 340 |
| DraI | TTTAAA | 1 cut(s) | 405 |
| Eam1104I | CTCTTC | 1 cut(s) | 330 |
| EarI | CTCTTC | 1 cut(s) | 330 |
| Eco57I | CTGAAG | 2 cut(s) | 162, 351 |
| EcoRI | GAATTC | 1 cut(s) | 136 |
| FaeI | CATG | 1 cut(s) | 68 |
| FaiI | YATR | 3 cut(s) | 45, 66, 422 |
| FatI | CATG | 1 cut(s) | 64 |
| FspBI | CTAG | 1 cut(s) | 219 |
| HaeIII | GGCC | 1 cut(s) | 98 |
| Hin1II | CATG | 1 cut(s) | 68 |
| HindIII | AAGCTT | 1 cut(s) | 39 |
| HinfI | GANTC | 1 cut(s) | 433 |
| HphI | GGTGA | 1 cut(s) | 167 |
| Hpy166II | GTNNAC | 1 cut(s) | 303 |
| Hpy188I | TCNGA | 1 cut(s) | 142 |
| Hpy188III | TCNNGA | 1 cut(s) | 329 |
| Hpy8I | GTNNAC | 1 cut(s) | 303 |
| HpyAV | CCTTC | 4 cut(s) | 26, 88, 257, 378 |
| HpyCH4V | TGCA | 1 cut(s) | 125 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 95 |
| HpyF3I | CTNAG | 1 cut(s) | 90 |
| Hsp92II | CATG | 1 cut(s) | 68 |
| Kzo9I | GATC | 2 cut(s) | 119, 340 |
| LpnPI | CCDG | 1 cut(s) | 342 |
| MaeI | CTAG | 1 cut(s) | 219 |
| MalI | GATC | 2 cut(s) | 121, 342 |
| MboI | GATC | 2 cut(s) | 119, 340 |
| MboII | GAAGA | 3 cut(s) | 281, 317, 442 |
| MflI | RGATCY | 1 cut(s) | 119 |
| MluCI | AATT | 4 cut(s) | 136, 192, 202, 347 |
| MnlI | CCTC | 4 cut(s) | 22, 67, 226, 322 |
| MseI | TTAA | 1 cut(s) | 404 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 95 |
| NdeII | GATC | 2 cut(s) | 119, 340 |
| NlaIII | CATG | 1 cut(s) | 68 |
| NlaIV | GGNNCC | 1 cut(s) | 366 |
| PfeI | GAWTC | 1 cut(s) | 433 |
| PspN4I | GGNNCC | 1 cut(s) | 366 |
| PstI | CTGCAG | 1 cut(s) | 127 |
| PsuI | RGATCY | 1 cut(s) | 119 |
| RsaI | GTAC | 3 cut(s) | 14, 132, 398 |
| RsaNI | GTAC | 3 cut(s) | 13, 131, 397 |
| SaqAI | TTAA | 1 cut(s) | 404 |
| Sau3AI | GATC | 2 cut(s) | 119, 340 |
| SetI | ASST | 5 cut(s) | 18, 43, 61, 337, 377 |
| SfcI | CTRYAG | 1 cut(s) | 123 |
| Sse9I | AATT | 4 cut(s) | 136, 192, 202, 347 |
| SspMI | CTAG | 1 cut(s) | 219 |
| TaqI | TCGA | 1 cut(s) | 199 |
| TasI | AATT | 4 cut(s) | 136, 192, 202, 347 |
| TfiI | GAWTC | 1 cut(s) | 433 |
| Tru1I | TTAA | 1 cut(s) | 404 |
| Tru9I | TTAA | 1 cut(s) | 404 |
| TscAI | CASTG | 1 cut(s) | 313 |
| TspDTI | ATGAA | 3 cut(s) | 60, 81, 288 |
| TspGWI | ACGGA | 1 cut(s) | 394 |
| TspRI | CASTG | 1 cut(s) | 313 |
| XapI | RAATTY | 1 cut(s) | 136 |
| XspI | CTAG | 1 cut(s) | 219 |