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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 3 cut(s) | 129, 316, 418 |
| AclWI | GGATC | 1 cut(s) | 78 |
| AcsI | RAATTY | 3 cut(s) | 167, 259, 292 |
| AcuI | CTGAAG | 1 cut(s) | 66 |
| AgsI | TTSAA | 1 cut(s) | 457 |
| AleI | CACNNNNGTG | 1 cut(s) | 477 |
| AluBI | AGCT | 1 cut(s) | 464 |
| AluI | AGCT | 1 cut(s) | 464 |
| Alw21I | GWGCWC | 1 cut(s) | 426 |
| AlwI | GGATC | 1 cut(s) | 78 |
| ApoI | RAATTY | 3 cut(s) | 167, 259, 292 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 266, 298 |
| AsuHPI | GGTGA | 1 cut(s) | 263 |
| BbsI | GAAGAC | 1 cut(s) | 120 |
| Bbv12I | GWGCWC | 1 cut(s) | 426 |
| BccI | CCATC | 3 cut(s) | 50, 242, 418 |
| BfaI | CTAG | 2 cut(s) | 96, 502 |
| BisI | GCNGC | 2 cut(s) | 129, 317 |
| BlsI | GCNGC | 2 cut(s) | 130, 318 |
| BpiI | GAAGAC | 1 cut(s) | 120 |
| BsiHKAI | GWGCWC | 1 cut(s) | 426 |
| BsmI | GAATGC | 1 cut(s) | 194 |
| Bsp1286I | GDGCHC | 1 cut(s) | 426 |
| Bsp143I | GATC | 1 cut(s) | 70 |
| BspACI | CCGC | 3 cut(s) | 129, 316, 418 |
| BspPI | GGATC | 1 cut(s) | 78 |
| BssMI | GATC | 1 cut(s) | 70 |
| Bst6I | CTCTTC | 1 cut(s) | 341 |
| BstKTI | GATC | 1 cut(s) | 73 |
| BstMBI | GATC | 1 cut(s) | 70 |
| BstNSI | RCATGY | 1 cut(s) | 392 |
| BstV2I | GAAGAC | 1 cut(s) | 120 |
| CviAII | CATG | 2 cut(s) | 320, 389 |
| CviJI | RGCY | 2 cut(s) | 128, 464 |
| CviKI_1 | RGCY | 2 cut(s) | 128, 464 |
| DpnI | GATC | 1 cut(s) | 72 |
| DpnII | GATC | 1 cut(s) | 70 |
| Eam1104I | CTCTTC | 1 cut(s) | 341 |
| EarI | CTCTTC | 1 cut(s) | 341 |
| Eco57I | CTGAAG | 1 cut(s) | 66 |
| EcoT22I | ATGCAT | 2 cut(s) | 196, 207 |
| FaeI | CATG | 2 cut(s) | 323, 392 |
| FatI | CATG | 2 cut(s) | 319, 388 |
| FauI | CCCGC | 1 cut(s) | 411 |
| Fnu4HI | GCNGC | 2 cut(s) | 129, 317 |
| Fsp4HI | GCNGC | 2 cut(s) | 129, 317 |
| FspBI | CTAG | 2 cut(s) | 96, 502 |
| GluI | GCNGC | 2 cut(s) | 129, 317 |
| Hin1II | CATG | 2 cut(s) | 323, 392 |
| HinfI | GANTC | 3 cut(s) | 41, 457, 488 |
| HphI | GGTGA | 1 cut(s) | 263 |
| Hpy188I | TCNGA | 2 cut(s) | 46, 487 |
| Hpy188III | TCNNGA | 1 cut(s) | 285 |
| HpyCH4IV | ACGT | 1 cut(s) | 474 |
| HpyCH4V | TGCA | 3 cut(s) | 194, 205, 472 |
| HpySE526I | ACGT | 1 cut(s) | 474 |
| Hsp92II | CATG | 2 cut(s) | 323, 392 |
| Kzo9I | GATC | 1 cut(s) | 70 |
| LpnPI | CCDG | 2 cut(s) | 346, 474 |
| MaeI | CTAG | 2 cut(s) | 96, 502 |
| MaeII | ACGT | 1 cut(s) | 474 |
| MaeIII | GTNAC | 1 cut(s) | 232 |
| MalI | GATC | 1 cut(s) | 72 |
| MboI | GATC | 1 cut(s) | 70 |
| MboII | GAAGA | 5 cut(s) | 50, 125, 328, 449, 452 |
| MhlI | GDGCHC | 1 cut(s) | 426 |
| MluCI | AATT | 6 cut(s) | 82, 100, 167, 218, 259, 292 |
| MmeI | TCCRAC | 2 cut(s) | 163, 465 |
| MnlI | CCTC | 2 cut(s) | 61, 378 |
| Mph1103I | ATGCAT | 2 cut(s) | 196, 207 |
| MseI | TTAA | 3 cut(s) | 189, 225, 278 |
| MslI | CAYNNNNRTG | 1 cut(s) | 477 |
| Mva1269I | GAATGC | 1 cut(s) | 194 |
| NdeII | GATC | 1 cut(s) | 70 |
| NlaIII | CATG | 2 cut(s) | 323, 392 |
| NmuCI | GTSAC | 1 cut(s) | 232 |
| NsiI | ATGCAT | 2 cut(s) | 196, 207 |
| NspI | RCATGY | 1 cut(s) | 392 |
| OliI | CACNNNNGTG | 1 cut(s) | 477 |
| PctI | GAATGC | 1 cut(s) | 194 |
| PfeI | GAWTC | 3 cut(s) | 41, 457, 488 |
| PkrI | GCNGC | 2 cut(s) | 130, 318 |
| RseI | CAYNNNNRTG | 1 cut(s) | 477 |
| SaqAI | TTAA | 3 cut(s) | 189, 225, 278 |
| SatI | GCNGC | 2 cut(s) | 129, 317 |
| Sau3AI | GATC | 1 cut(s) | 70 |
| SduI | GDGCHC | 1 cut(s) | 426 |
| SetI | ASST | 3 cut(s) | 455, 466, 477 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 477 |
| Sse9I | AATT | 6 cut(s) | 82, 100, 167, 218, 259, 292 |
| SsiI | CCGC | 3 cut(s) | 129, 316, 418 |
| SspI | AATATT | 1 cut(s) | 144 |
| SspMI | CTAG | 2 cut(s) | 96, 502 |
| TaiI | ACGT | 1 cut(s) | 477 |
| TaqI | TCGA | 3 cut(s) | 284, 339, 396 |
| TasI | AATT | 6 cut(s) | 82, 100, 167, 218, 259, 292 |
| TauI | GCSGC | 2 cut(s) | 131, 319 |
| TfiI | GAWTC | 3 cut(s) | 41, 457, 488 |
| Tru1I | TTAA | 3 cut(s) | 189, 225, 278 |
| Tru9I | TTAA | 3 cut(s) | 189, 225, 278 |
| TseFI | GTSAC | 1 cut(s) | 232 |
| Tsp45I | GTSAC | 1 cut(s) | 232 |
| TspDTI | ATGAA | 3 cut(s) | 51, 126, 187 |
| TspGWI | ACGGA | 1 cut(s) | 357 |
| XapI | RAATTY | 3 cut(s) | 167, 259, 292 |
| XceI | RCATGY | 1 cut(s) | 392 |
| XspI | CTAG | 2 cut(s) | 96, 502 |
| Zsp2I | ATGCAT | 2 cut(s) | 196, 207 |