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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 424 |
| AclI | AACGTT | 1 cut(s) | 162 |
| AclWI | GGATC | 1 cut(s) | 395 |
| AcsI | RAATTY | 1 cut(s) | 345 |
| AfaI | GTAC | 1 cut(s) | 102 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 40 |
| AflIII | ACRYGT | 2 cut(s) | 103, 181 |
| AgsI | TTSAA | 3 cut(s) | 89, 331, 412 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 299, 331, 404, 436 |
| Alw26I | GTCTC | 2 cut(s) | 9, 291 |
| AlwI | GGATC | 1 cut(s) | 395 |
| ApoI | RAATTY | 1 cut(s) | 345 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 28, 60 |
| AspS9I | GGNCC | 1 cut(s) | 238 |
| AvaII | GGWCC | 1 cut(s) | 238 |
| BanI | GGYRCC | 1 cut(s) | 424 |
| BccI | CCATC | 2 cut(s) | 155, 215 |
| BcoDI | GTCTC | 2 cut(s) | 9, 291 |
| Bme18I | GGWCC | 1 cut(s) | 238 |
| BmgT120I | GGNCC | 1 cut(s) | 238 |
| BmiI | GGNNCC | 2 cut(s) | 226, 426 |
| BmsI | GCATC | 1 cut(s) | 72 |
| Bsa29I | ATCGAT | 1 cut(s) | 190 |
| BsaAI | YACGTR | 1 cut(s) | 104 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 40 |
| BseCI | ATCGAT | 1 cut(s) | 190 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 40 |
| BseMII | CTCAG | 1 cut(s) | 20 |
| Bsh1285I | CGRYCG | 1 cut(s) | 194 |
| BshNI | GGYRCC | 1 cut(s) | 424 |
| BshVI | ATCGAT | 1 cut(s) | 190 |
| BsiEI | CGRYCG | 1 cut(s) | 194 |
| BslFI | GGGAC | 1 cut(s) | 147 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 40 |
| BsmAI | GTCTC | 2 cut(s) | 9, 291 |
| BsmFI | GGGAC | 1 cut(s) | 147 |
| BsmI | GAATGC | 1 cut(s) | 418 |
| Bsp143I | GATC | 3 cut(s) | 187, 191, 400 |
| BspCNI | CTCAG | 1 cut(s) | 19 |
| BspDI | ATCGAT | 1 cut(s) | 190 |
| BspHI | TCATGA | 1 cut(s) | 388 |
| BspLI | GGNNCC | 2 cut(s) | 226, 426 |
| BspPI | GGATC | 1 cut(s) | 395 |
| BspT107I | GGYRCC | 1 cut(s) | 424 |
| BssMI | GATC | 3 cut(s) | 187, 191, 400 |
| Bst6I | CTCTTC | 1 cut(s) | 390 |
| BstBAI | YACGTR | 1 cut(s) | 104 |
| BstDEI | CTNAG | 1 cut(s) | 6 |
| BstKTI | GATC | 3 cut(s) | 190, 194, 403 |
| BstMAI | GTCTC | 2 cut(s) | 9, 291 |
| BstMBI | GATC | 3 cut(s) | 187, 191, 400 |
| BstMCI | CGRYCG | 1 cut(s) | 194 |
| Bsu15I | ATCGAT | 1 cut(s) | 190 |
| BsuTUI | ATCGAT | 1 cut(s) | 190 |
| BtsI | GCAGTG | 1 cut(s) | 366 |
| BtsIMutI | CAGTG | 1 cut(s) | 366 |
| CciI | TCATGA | 1 cut(s) | 388 |
| Cfr13I | GGNCC | 1 cut(s) | 238 |
| ClaI | ATCGAT | 1 cut(s) | 190 |
| Csp6I | GTAC | 1 cut(s) | 101 |
| CviAII | CATG | 4 cut(s) | 64, 212, 389, 436 |
| CviQI | GTAC | 1 cut(s) | 101 |
| DdeI | CTNAG | 1 cut(s) | 6 |
| DpnI | GATC | 3 cut(s) | 189, 193, 402 |
| DpnII | GATC | 3 cut(s) | 187, 191, 400 |
| Eam1104I | CTCTTC | 1 cut(s) | 390 |
| EarI | CTCTTC | 1 cut(s) | 390 |
| Eco47I | GGWCC | 1 cut(s) | 238 |
| FaeI | CATG | 4 cut(s) | 67, 215, 392, 439 |
| FaiI | YATR | 6 cut(s) | 65, 213, 357, 359, 390, 437 |
| FaqI | GGGAC | 1 cut(s) | 147 |
| FatI | CATG | 4 cut(s) | 63, 211, 388, 435 |
| Hin1II | CATG | 4 cut(s) | 67, 215, 392, 439 |
| HinfI | GANTC | 1 cut(s) | 284 |
| Hpy166II | GTNNAC | 1 cut(s) | 363 |
| Hpy188I | TCNGA | 2 cut(s) | 35, 229 |
| Hpy188III | TCNNGA | 1 cut(s) | 389 |
| Hpy8I | GTNNAC | 1 cut(s) | 363 |
| Hpy99I | CGWCG | 2 cut(s) | 129, 132 |
| HpyAV | CCTTC | 2 cut(s) | 15, 438 |
| HpyCH4IV | ACGT | 4 cut(s) | 103, 136, 162, 181 |
| HpyCH4V | TGCA | 1 cut(s) | 98 |
| HpyF3I | CTNAG | 1 cut(s) | 6 |
| HpySE526I | ACGT | 4 cut(s) | 103, 136, 162, 181 |
| Hsp92II | CATG | 4 cut(s) | 67, 215, 392, 439 |
| Kzo9I | GATC | 3 cut(s) | 187, 191, 400 |
| LpnPI | CCDG | 1 cut(s) | 136 |
| LweI | GCATC | 1 cut(s) | 72 |
| MaeII | ACGT | 4 cut(s) | 103, 136, 162, 181 |
| MaeIII | GTNAC | 1 cut(s) | 163 |
| MalI | GATC | 3 cut(s) | 189, 193, 402 |
| MboI | GATC | 3 cut(s) | 187, 191, 400 |
| MboII | GAAGA | 5 cut(s) | 30, 343, 346, 349, 377 |
| MfeI | CAATTG | 1 cut(s) | 264 |
| MluCI | AATT | 4 cut(s) | 202, 264, 345, 407 |
| MlyI | GAGTC | 1 cut(s) | 293 |
| MmeI | TCCRAC | 1 cut(s) | 252 |
| MnlI | CCTC | 4 cut(s) | 20, 34, 229, 382 |
| MseI | TTAA | 2 cut(s) | 201, 293 |
| MslI | CAYNNNNRTG | 2 cut(s) | 180, 216 |
| MunI | CAATTG | 1 cut(s) | 264 |
| Mva1269I | GAATGC | 1 cut(s) | 418 |
| NdeII | GATC | 3 cut(s) | 187, 191, 400 |
| NlaIII | CATG | 4 cut(s) | 67, 215, 392, 439 |
| NlaIV | GGNNCC | 2 cut(s) | 226, 426 |
| PagI | TCATGA | 1 cut(s) | 388 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 114, 133, 187 |
| PctI | GAATGC | 1 cut(s) | 418 |
| Ple19I | CGATCG | 1 cut(s) | 194 |
| PleI | GAGTC | 1 cut(s) | 292 |
| PpsI | GAGTC | 1 cut(s) | 292 |
| Ppu21I | YACGTR | 1 cut(s) | 104 |
| Psp1406I | AACGTT | 1 cut(s) | 162 |
| PspN4I | GGNNCC | 2 cut(s) | 226, 426 |
| PspPI | GGNCC | 1 cut(s) | 238 |
| PvuI | CGATCG | 1 cut(s) | 194 |
| RsaI | GTAC | 1 cut(s) | 102 |
| RsaNI | GTAC | 1 cut(s) | 101 |
| RseI | CAYNNNNRTG | 2 cut(s) | 180, 216 |
| SaqAI | TTAA | 2 cut(s) | 201, 293 |
| Sau3AI | GATC | 3 cut(s) | 187, 191, 400 |
| Sau96I | GGNCC | 1 cut(s) | 238 |
| SchI | GAGTC | 1 cut(s) | 293 |
| SetI | ASST | 7 cut(s) | 12, 31, 106, 139, 165, 173, 184 |
| SfaNI | GCATC | 1 cut(s) | 72 |
| SinI | GGWCC | 1 cut(s) | 238 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 180, 216 |
| Sse9I | AATT | 4 cut(s) | 202, 264, 345, 407 |
| TaiI | ACGT | 4 cut(s) | 106, 139, 165, 184 |
| TaqI | TCGA | 4 cut(s) | 108, 117, 190, 194 |
| TasI | AATT | 4 cut(s) | 202, 264, 345, 407 |
| Tru1I | TTAA | 2 cut(s) | 201, 293 |
| Tru9I | TTAA | 2 cut(s) | 201, 293 |
| TscAI | CASTG | 1 cut(s) | 373 |
| TspDTI | ATGAA | 3 cut(s) | 356, 377, 405 |
| TspGWI | ACGGA | 1 cut(s) | 259 |
| TspRI | CASTG | 1 cut(s) | 373 |
| VpaK11BI | GGWCC | 1 cut(s) | 238 |
| XapI | RAATTY | 1 cut(s) | 345 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 218 |