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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 179 |
| AclWI | GGATC | 1 cut(s) | 259 |
| AcsI | RAATTY | 2 cut(s) | 281, 332 |
| AfaI | GTAC | 3 cut(s) | 130, 239, 376 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 83 |
| AgsI | TTSAA | 3 cut(s) | 64, 299, 395 |
| AhlI | ACTAGT | 1 cut(s) | 371 |
| AluBI | AGCT | 3 cut(s) | 67, 81, 313 |
| AluI | AGCT | 3 cut(s) | 67, 81, 313 |
| AlwI | GGATC | 1 cut(s) | 259 |
| ApeKI | GCWGC | 1 cut(s) | 40 |
| ApoI | RAATTY | 2 cut(s) | 281, 332 |
| AsuHPI | GGTGA | 1 cut(s) | 126 |
| BbvI | GCAGC | 1 cut(s) | 27 |
| BccI | CCATC | 1 cut(s) | 314 |
| BcuI | ACTAGT | 1 cut(s) | 371 |
| BfaI | CTAG | 3 cut(s) | 140, 270, 372 |
| BfmI | CTRYAG | 1 cut(s) | 93 |
| BisI | GCNGC | 1 cut(s) | 41 |
| BlsI | GCNGC | 1 cut(s) | 42 |
| BmcAI | AGTACT | 1 cut(s) | 376 |
| BmiI | GGNNCC | 2 cut(s) | 12, 275 |
| BsaJI | CCNNGG | 1 cut(s) | 260 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 83 |
| BseDI | CCNNGG | 1 cut(s) | 260 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 83 |
| BseXI | GCAGC | 1 cut(s) | 27 |
| BseYI | CCCAGC | 1 cut(s) | 81 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 83 |
| BsmI | GAATGC | 1 cut(s) | 358 |
| Bsp143I | GATC | 2 cut(s) | 160, 251 |
| BspLI | GGNNCC | 2 cut(s) | 12, 275 |
| BspPI | GGATC | 1 cut(s) | 259 |
| BssECI | CCNNGG | 1 cut(s) | 260 |
| BssMI | GATC | 2 cut(s) | 160, 251 |
| Bst4CI | ACNGT | 1 cut(s) | 97 |
| Bst6I | CTCTTC | 1 cut(s) | 200 |
| BstC8I | GCNNGC | 2 cut(s) | 69, 315 |
| BstDEI | CTNAG | 1 cut(s) | 378 |
| BstKTI | GATC | 2 cut(s) | 163, 254 |
| BstMBI | GATC | 2 cut(s) | 160, 251 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 17 |
| BstSFI | CTRYAG | 1 cut(s) | 93 |
| BstV1I | GCAGC | 1 cut(s) | 27 |
| BstX2I | RGATCY | 1 cut(s) | 251 |
| BstYI | RGATCY | 1 cut(s) | 251 |
| Cac8I | GCNNGC | 2 cut(s) | 69, 315 |
| Csp6I | GTAC | 3 cut(s) | 129, 238, 375 |
| CviJI | RGCY | 5 cut(s) | 11, 67, 71, 81, 313 |
| CviKI_1 | RGCY | 5 cut(s) | 11, 67, 71, 81, 313 |
| CviQI | GTAC | 3 cut(s) | 129, 238, 375 |
| DdeI | CTNAG | 1 cut(s) | 378 |
| DpnI | GATC | 2 cut(s) | 162, 253 |
| DpnII | GATC | 2 cut(s) | 160, 251 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 179 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 179 |
| Eam1104I | CTCTTC | 1 cut(s) | 200 |
| EarI | CTCTTC | 1 cut(s) | 200 |
| Fnu4HI | GCNGC | 1 cut(s) | 41 |
| Fsp4HI | GCNGC | 1 cut(s) | 41 |
| FspBI | CTAG | 3 cut(s) | 140, 270, 372 |
| GluI | GCNGC | 1 cut(s) | 41 |
| GsaI | CCCAGC | 1 cut(s) | 85 |
| HinfI | GANTC | 2 cut(s) | 101, 180 |
| HphI | GGTGA | 1 cut(s) | 126 |
| Hpy188I | TCNGA | 4 cut(s) | 31, 264, 331, 367 |
| Hpy188III | TCNNGA | 1 cut(s) | 197 |
| HpyCH4III | ACNGT | 1 cut(s) | 97 |
| HpyCH4V | TGCA | 1 cut(s) | 344 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 17 |
| HpyF3I | CTNAG | 1 cut(s) | 378 |
| Kzo9I | GATC | 2 cut(s) | 160, 251 |
| LmnI | GCTCC | 1 cut(s) | 16 |
| LpnPI | CCDG | 5 cut(s) | 33, 53, 67, 210, 315 |
| Lsp1109I | GCAGC | 1 cut(s) | 27 |
| MaeI | CTAG | 3 cut(s) | 140, 270, 372 |
| MalI | GATC | 2 cut(s) | 162, 253 |
| MboI | GATC | 2 cut(s) | 160, 251 |
| MboII | GAAGA | 2 cut(s) | 155, 217 |
| MflI | RGATCY | 1 cut(s) | 251 |
| MluCI | AATT | 3 cut(s) | 281, 332, 386 |
| MlyI | GAGTC | 1 cut(s) | 189 |
| MmeI | TCCRAC | 1 cut(s) | 38 |
| MnlI | CCTC | 5 cut(s) | 80, 136, 203, 270, 376 |
| Mva1269I | GAATGC | 1 cut(s) | 358 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 17 |
| NdeII | GATC | 2 cut(s) | 160, 251 |
| NlaIV | GGNNCC | 2 cut(s) | 12, 275 |
| PctI | GAATGC | 1 cut(s) | 358 |
| PfeI | GAWTC | 1 cut(s) | 101 |
| PkrI | GCNGC | 1 cut(s) | 42 |
| PleI | GAGTC | 1 cut(s) | 188 |
| PpsI | GAGTC | 1 cut(s) | 188 |
| PspFI | CCCAGC | 1 cut(s) | 81 |
| PspN4I | GGNNCC | 2 cut(s) | 12, 275 |
| PsuI | RGATCY | 1 cut(s) | 251 |
| RsaI | GTAC | 3 cut(s) | 130, 239, 376 |
| RsaNI | GTAC | 3 cut(s) | 129, 238, 375 |
| SatI | GCNGC | 1 cut(s) | 41 |
| Sau3AI | GATC | 2 cut(s) | 160, 251 |
| ScaI | AGTACT | 1 cut(s) | 376 |
| SchI | GAGTC | 1 cut(s) | 189 |
| SetI | ASST | 9 cut(s) | 69, 83, 91, 147, 214, 262, 275, 304, 315 |
| SfcI | CTRYAG | 1 cut(s) | 93 |
| SpeI | ACTAGT | 1 cut(s) | 371 |
| Sse9I | AATT | 3 cut(s) | 281, 332, 386 |
| SspMI | CTAG | 3 cut(s) | 140, 270, 372 |
| TaaI | ACNGT | 1 cut(s) | 97 |
| TaqI | TCGA | 1 cut(s) | 104 |
| TasI | AATT | 3 cut(s) | 281, 332, 386 |
| TatI | WGTACW | 3 cut(s) | 128, 237, 374 |
| TfiI | GAWTC | 1 cut(s) | 101 |
| TseI | GCWGC | 1 cut(s) | 40 |
| TspDTI | ATGAA | 1 cut(s) | 176 |
| XapI | RAATTY | 2 cut(s) | 281, 332 |
| XspI | CTAG | 3 cut(s) | 140, 270, 372 |
| ZrmI | AGTACT | 1 cut(s) | 376 |