MD01G1134700.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000305
Physical Location & Seq
Forward (+)
24437036 .. 24441384
4349 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1134700.v1.1.491

Sequence Viewer

Length: 594 bp
ATGACCTACAATGCCAAACTTTCTGATTTTGGTCTAGCCAAGGATGGCCCAGCCGGTGATAAAAGCCATGTCTCAACAAGAGTCATGGGGACATATGGTTATGCAGCGCCCGAGTATATGTCCACAGGTCATTTAACTGCGAGAAGTGACGTGTATAGCTTTGGGGTTGTTATGCTCGAACTATTGTCTGGCAGACGAGTTGTGGACAAGAACCGGCCATCGGGAGAACACAATTTAGTTGAATGGGCCAAACCTTACCTTGCAAGCAAACGCAAAGTTATTCAAATTTTTGACGGTCGTATTGAAGGCCAGTACTCTCTGGATGAAGCTCTCAGAGCTGTTAACCTTGCAATTCAATGCTTAGCTGTAGAACCCAAGTCTAGGCCAACCATGGATGACGTGGTAAAAGCGTTGGAGCAACTTCAGGAGCCCAGTGACTCGGAGGGCTCAGGTATCTTCCAAAATGAACATCGCCCAAACCCTCATGCAAATTCAAACCACGCCCCCAAACATCGCAGACACAGCATCAACGGAGTCAACAATGCAGCAACTCCTCATCGTCCCAGGGCATCCGCTTCCCTCACCCCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0035821 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0046777 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0060089 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0075136 GO:0080134 GO:0098542 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

198

Amino Acids

21.62

Weight (kDa)

8.99

Isoelectric Point (pI)

40.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 136 5.1e-17 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 4 - 138 4.3e-14 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000305)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39660 AT3G55450 AT3G55450
fragaria_vesca FvH4_7g22540 FvH4_7g22540 FvH4_7g22540 FvH4_7g22540 FvH4_7g22550 FvH4_7g22550 FvH4_7g22590 FvH4_7g22592 FvH4_7g22592 FvH4_7g22593 FvH4_7g22593 FvH4_7g22593
malus_domestica MD01G1134700.v1.1 MD07G1199400.v1.1
prunus_persica Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1
pyrus_communis pycom01g15540 pycom07g18630
rosa_chinensis RchiOBHm_Chr1g0367221 RchiOBHm_Chr1g0367231 RchiOBHm_Chr1g0367251 RchiOBHm_Chr1g0367261 RchiOBHm_Chr1g0367311 RchiOBHm_Chr1g0367321 RchiOBHm_Chr1g0367351 RchiOBHm_Chr1g0367401 RchiOBHm_Chr1g0367411 RchiOBHm_Chr1g0367581 RchiOBHm_Chr5g0029531
rosa_laevigata RLG00000001400 RLG00000024338 RLG00000027271 RLG00000027274 RLG00000027277 RLG00000027278 RLG00000027280 RLG00000027281 RLG00000027282 RLG00000027285 RLG00000027286 RLG00000027288 RLG00000033176
rosa_multiflora Rmu_sc0002690.1_g000002 Rmu_sc0004181.1_g000007 Rmu_sc0008470.1_g000013 Rmu_sc0008470.1_g000015 Rmu_sc0009775.1_g000001 Rmu_sc0009775.1_g000003 Rmu_sc0009775.1_g000004 Rmu_sc0009775.1_g000005 Rmu_sc0011000.1_g000008 Rmu_sc0014291.1_g000001 Rmu_sc0014291.1_g000005 Rmu_sc0014291.1_g000008
rosa_roxburghii Rroxscaffold_1G00050440 Rroxscaffold_4G00289340 Rroxscaffold_4G00289350 Rroxscaffold_4G00289380 Rroxscaffold_4G00289400 Rroxscaffold_4G00289410 Rroxscaffold_4G00289450 Rroxscaffold_4G00289490 Rroxscaffold_4G00289500 Rroxscaffold_4G00289510 Rroxscaffold_6G00420130 Rroxscaffold_6G00421370
rosa_rugosa Rorug01G0335600.1 Rorug01G0335700 Rorug01G0335800 Rorug01G0335800 Rorug01G0335800 Rorug01G0335800 Rorug01G0335900 Rorug01G0335900 Rorug01G0336000 Rorug01G0336000 Rorug05G0114700 Rorug05G0114800
rosa_samantha Rh1AG342300 Rh1AG342500 Rh1AG342700 Rh1BG302700 Rh1BG303000 Rh1BG303100 Rh1BG303400 Rh1BG303500 Rh1BG303800 Rh1BG304100 Rh1CG319200 Rh1CG319400 Rh1CG319800 Rh1CG319900 Rh1DG335300 Rh1DG335500 Rh1DG335900 Rh1DG336200 Rh2AG106400 Rh3DG307700 Rh4CG281300 Rh5AG206700 Rh5CG227400 Rh5DG209100 Rh7CG338300
rosa_wichuraiana Rw1G030420 Rw1G030430 Rw1G030460 Rw1G030470 Rw1G030480 Rw1G030510 Rw1G030520 Rw5G018800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 573
AcoI YGGCCR 1 cut(s) 215
AcsI RAATTY 2 cut(s) 285, 490
AcuI CTGAAG 1 cut(s) 407
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 2 cut(s) 220, 381
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 5 cut(s) 242, 284, 305, 356, 495
AjiI CACGTC 2 cut(s) 151, 400
AjnI CCWGG 1 cut(s) 563
AluBI AGCT 4 cut(s) 159, 329, 338, 365
AluI AGCT 4 cut(s) 159, 329, 338, 365
Alw26I GTCTC 1 cut(s) 76
Ama87I CYCGRG 1 cut(s) 110
AoxI GGCC 5 cut(s) 46, 215, 246, 307, 383
ApeKI GCWGC 2 cut(s) 104, 545
ApoI RAATTY 2 cut(s) 285, 490
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 2 cut(s) 47, 246
AsuHPI GGTGA 2 cut(s) 68, 574
AvaI CYCGRG 1 cut(s) 110
BanII GRGCYC 2 cut(s) 432, 449
BbvI GCAGC 2 cut(s) 116, 557
BccI CCATC 2 cut(s) 38, 226
BciT130I CCWGG 1 cut(s) 565
BcoDI GTCTC 1 cut(s) 76
BfaI CTAG 2 cut(s) 35, 381
BfmI CTRYAG 1 cut(s) 366
BfoI RGCGCY 1 cut(s) 110
BisI GCNGC 2 cut(s) 105, 546
BlpI GCTNAGC 1 cut(s) 361
BlsI GCNGC 2 cut(s) 106, 547
BmcAI AGTACT 1 cut(s) 314
Bme1390I CCNGG 1 cut(s) 565
BmeT110I CYCGRG 1 cut(s) 110
BmgBI CACGTC 2 cut(s) 151, 400
BmgT120I GGNCC 2 cut(s) 47, 246
BmiI GGNNCC 1 cut(s) 429
BmrFI CCNGG 1 cut(s) 565
BmrI ACTGGG 1 cut(s) 426
BmsI GCATC 2 cut(s) 534, 578
BmuI ACTGGG 1 cut(s) 426
Bpu10I CCTNAGC 1 cut(s) 448
Bpu1102I GCTNAGC 1 cut(s) 361
BsaJI CCNNGG 4 cut(s) 39, 390, 563, 564
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 381
Bse118I RCCGGY 2 cut(s) 53, 213
Bse1I ACTGG 2 cut(s) 310, 432
BseBI CCWGG 1 cut(s) 565
BseDI CCNNGG 4 cut(s) 39, 390, 563, 564
BseGI GGATG 4 cut(s) 49, 328, 400, 569
BseLI CCNNNNNNNGG 2 cut(s) 220, 381
BseMII CTCAG 2 cut(s) 346, 462
BseNI ACTGG 2 cut(s) 310, 432
BseRI GAGGAG 1 cut(s) 543
BseXI GCAGC 2 cut(s) 116, 557
BseYI CCCAGC 2 cut(s) 49, 586
Bsh1285I CGRYCG 1 cut(s) 298
BshFI GGCC 5 cut(s) 48, 217, 248, 309, 385
BsiEI CGRYCG 1 cut(s) 298
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 2 cut(s) 54, 214
BslFI GGGAC 2 cut(s) 103, 546
BslI CCNNNNNNNGG 2 cut(s) 220, 381
BsmAI GTCTC 1 cut(s) 76
BsmFI GGGAC 2 cut(s) 103, 546
BsnI GGCC 5 cut(s) 48, 217, 248, 309, 385
BsoBI CYCGRG 1 cut(s) 110
Bsp1286I GDGCHC 2 cut(s) 432, 449
Bsp1720I GCTNAGC 1 cut(s) 361
Bsp19I CCATGG 1 cut(s) 390
BspACI CCGC 1 cut(s) 573
BspANI GGCC 5 cut(s) 48, 217, 248, 309, 385
BspCNI CTCAG 2 cut(s) 345, 461
BspLI GGNNCC 1 cut(s) 429
BsrFI RCCGGY 2 cut(s) 53, 213
BsrI ACTGG 2 cut(s) 310, 432
BssAI RCCGGY 2 cut(s) 53, 213
BssECI CCNNGG 4 cut(s) 39, 390, 563, 564
BssT1I CCWWGG 2 cut(s) 39, 390
Bst2UI CCWGG 1 cut(s) 565
Bst4CI ACNGT 1 cut(s) 296
BstC8I GCNNGC 1 cut(s) 265
BstDEI CTNAG 3 cut(s) 332, 361, 448
BstDSI CCRYGG 1 cut(s) 390
BstF5I GGATG 4 cut(s) 49, 328, 400, 569
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 1 cut(s) 109
BstMAI GTCTC 1 cut(s) 76
BstMCI CGRYCG 1 cut(s) 298
BstMWI GCNNNNNNNGC 2 cut(s) 335, 522
BstNI CCWGG 1 cut(s) 565
BstSCI CCNGG 1 cut(s) 563
BstSFI CTRYAG 1 cut(s) 366
BstV1I GCAGC 2 cut(s) 116, 557
BsuRI GGCC 5 cut(s) 48, 217, 248, 309, 385
BtgI CCRYGG 1 cut(s) 390
BtgZI GCGATG 2 cut(s) 455, 497
BtrI CACGTC 2 cut(s) 151, 400
BtsCI GGATG 4 cut(s) 49, 328, 400, 569
BtsIMutI CAGTG 1 cut(s) 439
Cac8I GCNNGC 1 cut(s) 265
CfoI GCGC 1 cut(s) 109
Cfr10I RCCGGY 2 cut(s) 53, 213
Cfr13I GGNCC 2 cut(s) 47, 246
Csp6I GTAC 1 cut(s) 313
CviAII CATG 5 cut(s) 68, 85, 391, 485, 591
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 3 cut(s) 332, 361, 448
EaeI YGGCCR 1 cut(s) 215
Eco130I CCWWGG 2 cut(s) 39, 390
Eco24I GRGCYC 2 cut(s) 432, 449
Eco57I CTGAAG 1 cut(s) 407
Eco88I CYCGRG 1 cut(s) 110
EcoRII CCWGG 1 cut(s) 563
EcoT14I CCWWGG 2 cut(s) 39, 390
EcoT38I GRGCYC 2 cut(s) 432, 449
ErhI CCWWGG 2 cut(s) 39, 390
FaeI CATG 5 cut(s) 71, 88, 394, 488, 594
FaqI GGGAC 2 cut(s) 103, 546
FatI CATG 5 cut(s) 67, 84, 390, 484, 590
FauNDI CATATG 1 cut(s) 94
Fnu4HI GCNGC 2 cut(s) 105, 546
FokI GGATG 4 cut(s) 56, 335, 407, 556
FriOI GRGCYC 2 cut(s) 432, 449
Fsp4HI GCNGC 2 cut(s) 105, 546
FspBI CTAG 2 cut(s) 35, 381
GlaI GCGC 1 cut(s) 108
GluI GCNGC 2 cut(s) 105, 546
GsaI CCCAGC 2 cut(s) 53, 590
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 5 cut(s) 48, 217, 248, 309, 385
HapII CCGG 2 cut(s) 54, 214
HhaI GCGC 1 cut(s) 109
Hin1II CATG 5 cut(s) 71, 88, 394, 488, 594
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HincII GTYRAC 2 cut(s) 343, 538
HindII GTYRAC 2 cut(s) 343, 538
HinfI GANTC 3 cut(s) 81, 437, 534
HpaI GTTAAC 1 cut(s) 343
HpaII CCGG 2 cut(s) 54, 214
HphI GGTGA 2 cut(s) 68, 574
Hpy166II GTNNAC 4 cut(s) 123, 205, 343, 538
Hpy188I TCNGA 3 cut(s) 25, 335, 442
Hpy188III TCNNGA 3 cut(s) 222, 320, 425
Hpy8I GTNNAC 4 cut(s) 123, 205, 343, 538
HpyAV CCTTC 1 cut(s) 299
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4IV ACGT 2 cut(s) 150, 399
HpyCH4V TGCA 5 cut(s) 104, 263, 350, 488, 545
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 522
HpyF3I CTNAG 3 cut(s) 332, 361, 448
HpySE526I ACGT 2 cut(s) 150, 399
Hsp92II CATG 5 cut(s) 71, 88, 394, 488, 594
HspAI GCGC 1 cut(s) 107
KspAI GTTAAC 1 cut(s) 343
LmnI GCTCC 2 cut(s) 415, 427
Lsp1109I GCAGC 2 cut(s) 116, 557
LweI GCATC 2 cut(s) 534, 578
MaeI CTAG 2 cut(s) 35, 381
MaeII ACGT 2 cut(s) 150, 399
MaeIII GTNAC 2 cut(s) 146, 434
MboII GAAGA 1 cut(s) 448
MhlI GDGCHC 2 cut(s) 432, 449
MluCI AATT 4 cut(s) 232, 285, 351, 490
MlyI GAGTC 3 cut(s) 90, 431, 543
MmeI TCCRAC 1 cut(s) 393
MnlI CCTC 4 cut(s) 436, 492, 564, 590
MseI TTAA 2 cut(s) 134, 342
MspI CCGG 2 cut(s) 54, 214
MspR9I CCNGG 1 cut(s) 565
MvaI CCWGG 1 cut(s) 565
MwoI GCNNNNNNNGC 2 cut(s) 335, 522
NcoI CCATGG 1 cut(s) 390
NdeI CATATG 1 cut(s) 94
NlaIII CATG 5 cut(s) 71, 88, 394, 488, 594
NlaIV GGNNCC 1 cut(s) 429
NmuCI GTSAC 2 cut(s) 146, 434
PasI CCCWGGG 1 cut(s) 564
PkrI GCNGC 2 cut(s) 106, 547
PleI GAGTC 3 cut(s) 89, 431, 542
PpsI GAGTC 3 cut(s) 89, 431, 542
Psp6I CCWGG 1 cut(s) 563
PspFI CCCAGC 2 cut(s) 49, 586
PspGI CCWGG 1 cut(s) 563
PspN4I GGNNCC 1 cut(s) 429
PspPI GGNCC 2 cut(s) 47, 246
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
SaqAI TTAA 2 cut(s) 134, 342
SatI GCNGC 2 cut(s) 105, 546
Sau96I GGNCC 2 cut(s) 47, 246
ScaI AGTACT 1 cut(s) 314
SchI GAGTC 3 cut(s) 90, 431, 543
ScrFI CCNGG 1 cut(s) 565
SduI GDGCHC 2 cut(s) 432, 449
SfaNI GCATC 2 cut(s) 534, 578
SfcI CTRYAG 1 cut(s) 366
Sse9I AATT 4 cut(s) 232, 285, 351, 490
SsiI CCGC 1 cut(s) 573
SspMI CTAG 2 cut(s) 35, 381
StyD4I CCNGG 1 cut(s) 563
StyI CCWWGG 2 cut(s) 39, 390
TaaI ACNGT 1 cut(s) 296
TaiI ACGT 2 cut(s) 153, 402
TaqI TCGA 1 cut(s) 177
TasI AATT 4 cut(s) 232, 285, 351, 490
TatI WGTACW 1 cut(s) 312
Tru1I TTAA 2 cut(s) 134, 342
Tru9I TTAA 2 cut(s) 134, 342
TscAI CASTG 1 cut(s) 439
TseFI GTSAC 2 cut(s) 146, 434
TseI GCWGC 2 cut(s) 104, 545
Tsp45I GTSAC 2 cut(s) 146, 434
TspDTI ATGAA 2 cut(s) 339, 480
TspGWI ACGGA 1 cut(s) 546
TspRI CASTG 1 cut(s) 439
XapI RAATTY 2 cut(s) 285, 490
XcmI CCANNNNNNNNNTGG 1 cut(s) 397
XspI CTAG 2 cut(s) 35, 381
ZrmI AGTACT 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.