RLG00000027280
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000305
Physical Location & Seq
Reverse (-)
10243154 .. 10248098
4945 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027280

Sequence Viewer

Length: 1446 bp
ATGTTGTCTGGAAGACGAGCTGTGGACAAGAACCGGCCTACAGGAGAACACAGCTTAGTTGAGTGGGCCAAACCTTACCTTGCCAGCAAACGCAGAGTTCTCCAAGTTCTTGATCCTCGTATTGATGGGCAGTACTCTGTGGCTGTAGCTCTTAAAGCAGTTAACCTTGCAATTCAATGCTTATCAACAGAACCCAAGTTTAGGCCAATCATGCATGAATATGTGGTAAAAGCGTTAGAGCAGCTTCAGGAACCTGGAAGACATGGAGGGTTTAGTGGTAGCATTCCTTGCTTGCCGATATTGGGCTCTGCTTCAAAGAGCAGGGTTTCAAACGGAACTAGAATCAGAGCTGAGAGTCTGTGTCACAGAGTGCCTTCAACTCCTCGGACAGAGGTTGAGATCTTGCTAAAGAGCTTCTTTTTTAATGAACTGAAAACCGCCACCAGGAACTTTCGGCCTGATAATGTGGTGGGTGAGGGTGGTTTTGGTACTGTCTTTAAGGGGTGGATTGATCAGAATTCATTAGCAGCTGCCAAGCCTGGTAAAGGAATGGCTGTTGCTATAAAGAGGCTTAACCAAGAAAGTTATCAGGGTCGGGAGGAATGGTTTAAAGAAATTTACTACCTTGGACAGCTGCATCACCCAAATCTTGTGAAGTTGATAGGCTATTGTTCAGAGGATAACGAACTGCTTCTGGTGTATGAATTTGTGCCCCGTGGTAGTTTGGATAATCATATATTTAGAAGGAGTTCTCACTTTCAACCACTTTCATGGACTTTTCGTATGAAGATTGCCCTTGGTGCTGCGAAGGCTCTAGCATTTCTTCACAATGAAGCAAAAGTTATATATCGTGACTTTAAAACAGCTAATATCCTGTTGGATTCAACCTACAATGTCAAGCTCTCTGATTTTGGTTTGGCCATGGATGGGCGATTAATTGACAGACACCCAGGTGCCAAAACAAGGGTCATGGGCACATATGGATGTGCAGCTCCCAAGTATATAGAAACAAGGGTCATGGGCACACATGGATATGCAGCTCCTGAGTATATAGCTACAGGTCATCTAAACACCAAATGTGATGTGTATGGTTTTGGAGCTGTTATGCTCGAATTGTTGTCAGGAAAACGAGTTTTTGACCAGAGCCGACCACCCGGGGAACAGAAATTAGTTAAATGGGCCAAACCTTACCTTGTCAGCAAACAGAGAGTCCTCCAAATTTTTGATTCTCATATTAAAGGCCAGTTTTCTGTGGCCAGAGCTCTTAAAGCAGTTGACCTTACATTTCTTTGCCTATCGAGAGATCCCAAGCTTAGGCCAAACATGAATAATGTGGTAAAAACATTGGAGAAGCTTCAGGAATCCAGTGACATGGATGGCTTGGGGATCTCTCAAAATAAACGTCGCCAATATCCACATGGCAGTTTAATCAATGGTCCCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0035821 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0046777 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0060089 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0075136 GO:0080134 GO:0098542 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

482

Amino Acids

53.98

Weight (kDa)

9.72

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 154 - 447 1.9e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 155 - 449 3.4e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000305)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39660 AT3G55450 AT3G55450
fragaria_vesca FvH4_7g22540 FvH4_7g22540 FvH4_7g22540 FvH4_7g22540 FvH4_7g22550 FvH4_7g22550 FvH4_7g22590 FvH4_7g22592 FvH4_7g22592 FvH4_7g22593 FvH4_7g22593 FvH4_7g22593
malus_domestica MD01G1134700.v1.1 MD07G1199400.v1.1
prunus_persica Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1
pyrus_communis pycom01g15540 pycom07g18630
rosa_chinensis RchiOBHm_Chr1g0367221 RchiOBHm_Chr1g0367231 RchiOBHm_Chr1g0367251 RchiOBHm_Chr1g0367261 RchiOBHm_Chr1g0367311 RchiOBHm_Chr1g0367321 RchiOBHm_Chr1g0367351 RchiOBHm_Chr1g0367401 RchiOBHm_Chr1g0367411 RchiOBHm_Chr1g0367581 RchiOBHm_Chr5g0029531
rosa_laevigata RLG00000001400 RLG00000024338 RLG00000027271 RLG00000027274 RLG00000027277 RLG00000027278 RLG00000027280 RLG00000027281 RLG00000027282 RLG00000027285 RLG00000027286 RLG00000027288 RLG00000033176
rosa_multiflora Rmu_sc0002690.1_g000002 Rmu_sc0004181.1_g000007 Rmu_sc0008470.1_g000013 Rmu_sc0008470.1_g000015 Rmu_sc0009775.1_g000001 Rmu_sc0009775.1_g000003 Rmu_sc0009775.1_g000004 Rmu_sc0009775.1_g000005 Rmu_sc0011000.1_g000008 Rmu_sc0014291.1_g000001 Rmu_sc0014291.1_g000005 Rmu_sc0014291.1_g000008
rosa_roxburghii Rroxscaffold_1G00050440 Rroxscaffold_4G00289340 Rroxscaffold_4G00289350 Rroxscaffold_4G00289380 Rroxscaffold_4G00289400 Rroxscaffold_4G00289410 Rroxscaffold_4G00289450 Rroxscaffold_4G00289490 Rroxscaffold_4G00289500 Rroxscaffold_4G00289510 Rroxscaffold_6G00420130 Rroxscaffold_6G00421370
rosa_rugosa Rorug01G0335600.1 Rorug01G0335700 Rorug01G0335800 Rorug01G0335800 Rorug01G0335800 Rorug01G0335800 Rorug01G0335900 Rorug01G0335900 Rorug01G0336000 Rorug01G0336000 Rorug05G0114700 Rorug05G0114800
rosa_samantha Rh1AG342300 Rh1AG342500 Rh1AG342700 Rh1BG302700 Rh1BG303000 Rh1BG303100 Rh1BG303400 Rh1BG303500 Rh1BG303800 Rh1BG304100 Rh1CG319200 Rh1CG319400 Rh1CG319800 Rh1CG319900 Rh1DG335300 Rh1DG335500 Rh1DG335900 Rh1DG336200 Rh2AG106400 Rh3DG307700 Rh4CG281300 Rh5AG206700 Rh5CG227400 Rh5DG209100 Rh7CG338300
rosa_wichuraiana Rw1G030420 Rw1G030430 Rw1G030460 Rw1G030470 Rw1G030480 Rw1G030510 Rw1G030520 Rw5G018800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 953
AciI CCGC 1 cut(s) 438
AclWI GGATC 3 cut(s) 107, 1298, 1394
AcoI YGGCCR 2 cut(s) 918, 1254
AcsI RAATTY 4 cut(s) 517, 615, 704, 1218
AcuI CTGAAG 2 cut(s) 230, 1340
AdeI CACNNNGTG 1 cut(s) 370
AfaI GTAC 2 cut(s) 134, 490
AfiI CCNNNNNNNGG 7 cut(s) 201, 302, 444, 545, 927, 963, 1314
AgsI TTSAA 6 cut(s) 176, 315, 330, 378, 761, 885
AjnI CCWGG 4 cut(s) 253, 443, 538, 949
AleI CACNNNNGTG 1 cut(s) 951
Alw21I GWGCWC 1 cut(s) 1264
AlwI GGATC 3 cut(s) 107, 1298, 1394
AlwNI CAGNNNCTG 1 cut(s) 1043
Ama87I CYCGRG 1 cut(s) 1154
AoxI GGCC 9 cut(s) 35, 66, 203, 455, 918, 1179, 1240, 1254, 1316
ApeKI GCWGC 7 cut(s) 241, 527, 530, 634, 803, 989, 1037
ApoI RAATTY 4 cut(s) 517, 615, 704, 1218
ArsI GACNNNNNNTTYG 6 cut(s) 951, 983, 1194, 1226, 1387, 1419
AseI ATTAAT 1 cut(s) 935
Asp700I GAANNNNTTC 2 cut(s) 690, 748
AspS9I GGNCC 3 cut(s) 66, 1179, 1436
AsuC2I CCSGG 2 cut(s) 1155, 1156
AsuHPI GGTGA 2 cut(s) 485, 632
AvaI CYCGRG 1 cut(s) 1154
AvaII GGWCC 1 cut(s) 1436
BaeGI GKGCMC 3 cut(s) 714, 977, 1025
BalI TGGCCA 2 cut(s) 920, 1256
BanI GGYRCC 1 cut(s) 953
BanII GRGCYC 2 cut(s) 308, 1264
BbsI GAAGAC 2 cut(s) 19, 265
Bbv12I GWGCWC 1 cut(s) 1264
BbvI GCAGC 7 cut(s) 253, 517, 539, 621, 790, 1001, 1049
BccI CCATC 3 cut(s) 119, 920, 1370
BciT130I CCWGG 4 cut(s) 255, 445, 540, 951
BclI TGATCA 1 cut(s) 511
BcnI CCSGG 2 cut(s) 1155, 1156
BfaI CTAG 2 cut(s) 339, 815
BfmI CTRYAG 3 cut(s) 39, 144, 1056
BglII AGATCT 1 cut(s) 399
BisI GCNGC 7 cut(s) 242, 528, 531, 635, 804, 990, 1038
BlsI GCNGC 7 cut(s) 243, 529, 532, 636, 805, 991, 1039
BmcAI AGTACT 1 cut(s) 134
Bme1390I CCNGG 6 cut(s) 255, 445, 540, 951, 1155, 1156
Bme18I GGWCC 1 cut(s) 1436
BmeT110I CYCGRG 1 cut(s) 1154
BmgT120I GGNCC 3 cut(s) 66, 1179, 1436
BmiI GGNNCC 3 cut(s) 252, 955, 1438
BmrFI CCNGG 6 cut(s) 255, 445, 540, 951, 1155, 1156
BmsI GCATC 1 cut(s) 646
BpiI GAAGAC 2 cut(s) 19, 265
Bpu10I CCTNAGC 1 cut(s) 1313
BpuMI CCSGG 2 cut(s) 1155, 1156
BsaJI CCNNGG 8 cut(s) 383, 625, 715, 796, 921, 949, 1154, 1155
BsaXI ACNNNNNCTCC 2 cut(s) 590, 620
Bsc4I CCNNNNNNNGG 7 cut(s) 201, 302, 444, 545, 927, 963, 1314
Bse118I RCCGGY 1 cut(s) 33
Bse1I ACTGG 2 cut(s) 1243, 1365
BseBI CCWGG 4 cut(s) 255, 445, 540, 951
BseDI CCNNGG 8 cut(s) 383, 625, 715, 796, 921, 949, 1154, 1155
BseGI GGATG 3 cut(s) 931, 989, 1381
BseLI CCNNNNNNNGG 7 cut(s) 201, 302, 444, 545, 927, 963, 1314
BseMII CTCAG 2 cut(s) 342, 1035
BseNI ACTGG 2 cut(s) 1243, 1365
BseRI GAGGAG 1 cut(s) 372
BseSI GKGCMC 3 cut(s) 714, 977, 1025
BseXI GCAGC 7 cut(s) 253, 517, 539, 621, 790, 1001, 1049
BsgI GTGCAG 1 cut(s) 1008
BshFI GGCC 9 cut(s) 37, 68, 205, 457, 920, 1181, 1242, 1256, 1318
BshNI GGYRCC 1 cut(s) 953
BsiHKAI GWGCWC 1 cut(s) 1264
BsiHKCI CYCGRG 1 cut(s) 1154
BsiSI CCGG 2 cut(s) 34, 1155
BslFI GGGAC 1 cut(s) 1422
BslI CCNNNNNNNGG 7 cut(s) 201, 302, 444, 545, 927, 963, 1314
BsmFI GGGAC 1 cut(s) 1422
BsmI GAATGC 1 cut(s) 282
BsnI GGCC 9 cut(s) 37, 68, 205, 457, 920, 1181, 1242, 1256, 1318
BsoBI CYCGRG 1 cut(s) 1154
Bsp1286I GDGCHC 5 cut(s) 308, 714, 977, 1025, 1264
Bsp143I GATC 5 cut(s) 112, 399, 511, 1303, 1386
Bsp19I CCATGG 1 cut(s) 921
BspACI CCGC 1 cut(s) 438
BspANI GGCC 9 cut(s) 37, 68, 205, 457, 920, 1181, 1242, 1256, 1318
BspCNI CTCAG 2 cut(s) 343, 1036
BspLI GGNNCC 3 cut(s) 252, 955, 1438
BspPI GGATC 3 cut(s) 107, 1298, 1394
BspT107I GGYRCC 1 cut(s) 953
BsrFI RCCGGY 1 cut(s) 33
BsrI ACTGG 2 cut(s) 1243, 1365
BssAI RCCGGY 1 cut(s) 33
BssECI CCNNGG 8 cut(s) 383, 625, 715, 796, 921, 949, 1154, 1155
BssMI GATC 5 cut(s) 112, 399, 511, 1303, 1386
BssT1I CCWWGG 3 cut(s) 625, 796, 921
Bst2UI CCWGG 4 cut(s) 255, 445, 540, 951
Bst4CI ACNGT 1 cut(s) 493
BstAPI GCANNNNNTGC 1 cut(s) 288
BstC8I GCNNGC 2 cut(s) 85, 293
BstDEI CTNAG 4 cut(s) 55, 351, 1044, 1313
BstDSI CCRYGG 2 cut(s) 715, 921
BstENI CCTNNNNNAGG 1 cut(s) 543
BstF5I GGATG 3 cut(s) 931, 989, 1381
BstKTI GATC 5 cut(s) 115, 402, 514, 1306, 1389
BstMBI GATC 5 cut(s) 112, 399, 511, 1303, 1386
BstMWI GCNNNNNNNGC 6 cut(s) 155, 211, 288, 800, 809, 1268
BstNI CCWGG 4 cut(s) 255, 445, 540, 951
BstSCI CCNGG 6 cut(s) 253, 443, 538, 949, 1153, 1154
BstSFI CTRYAG 3 cut(s) 39, 144, 1056
BstSLI GKGCMC 3 cut(s) 714, 977, 1025
BstV1I GCAGC 7 cut(s) 253, 517, 539, 621, 790, 1001, 1049
BstV2I GAAGAC 2 cut(s) 19, 265
BstX2I RGATCY 3 cut(s) 399, 1303, 1386
BstXI CCANNNNNNTGG 2 cut(s) 771, 1372
BstYI RGATCY 3 cut(s) 399, 1303, 1386
BsuRI GGCC 9 cut(s) 37, 68, 205, 457, 920, 1181, 1242, 1256, 1318
BtgI CCRYGG 2 cut(s) 715, 921
BtsCI GGATG 3 cut(s) 931, 989, 1381
BtsIMutI CAGTG 1 cut(s) 1372
Cac8I GCNNGC 2 cut(s) 85, 293
CaiI CAGNNNCTG 1 cut(s) 1043
Cfr10I RCCGGY 1 cut(s) 33
Cfr13I GGNCC 3 cut(s) 66, 1179, 1436
Cfr9I CCCGGG 1 cut(s) 1154
Csp6I GTAC 2 cut(s) 133, 489
CviQI GTAC 2 cut(s) 133, 489
DdeI CTNAG 4 cut(s) 55, 351, 1044, 1313
DpnI GATC 5 cut(s) 114, 401, 513, 1305, 1388
DpnII GATC 5 cut(s) 112, 399, 511, 1303, 1386
DraI TTTAAA 2 cut(s) 610, 859
DraIII CACNNNGTG 1 cut(s) 370
EaeI YGGCCR 2 cut(s) 918, 1254
Ecl136II GAGCTC 1 cut(s) 1262
Eco130I CCWWGG 3 cut(s) 625, 796, 921
Eco24I GRGCYC 2 cut(s) 308, 1264
Eco47I GGWCC 1 cut(s) 1436
Eco53kI GAGCTC 1 cut(s) 1262
Eco57I CTGAAG 2 cut(s) 230, 1340
Eco88I CYCGRG 1 cut(s) 1154
EcoICRI GAGCTC 1 cut(s) 1262
EcoNI CCTNNNNNAGG 1 cut(s) 543
EcoRI GAATTC 1 cut(s) 517
EcoRII CCWGG 4 cut(s) 253, 443, 538, 949
EcoT14I CCWWGG 3 cut(s) 625, 796, 921
EcoT22I ATGCAT 1 cut(s) 216
EcoT38I GRGCYC 2 cut(s) 308, 1264
ErhI CCWWGG 3 cut(s) 625, 796, 921
FalI AAGNNNNNCTT 2 cut(s) 401, 433
FaqI GGGAC 1 cut(s) 1422
FauNDI CATATG 1 cut(s) 979
FbaI TGATCA 1 cut(s) 511
Fnu4HI GCNGC 7 cut(s) 242, 528, 531, 635, 804, 990, 1038
FokI GGATG 3 cut(s) 938, 996, 1388
FriOI GRGCYC 2 cut(s) 308, 1264
Fsp4HI GCNGC 7 cut(s) 242, 528, 531, 635, 804, 990, 1038
FspBI CTAG 2 cut(s) 339, 815
GluI GCNGC 7 cut(s) 242, 528, 531, 635, 804, 990, 1038
HaeIII GGCC 9 cut(s) 37, 68, 205, 457, 920, 1181, 1242, 1256, 1318
HapII CCGG 2 cut(s) 34, 1155
HincII GTYRAC 2 cut(s) 163, 1276
HindII GTYRAC 2 cut(s) 163, 1276
HindIII AAGCTT 2 cut(s) 1310, 1352
HinfI GANTC 6 cut(s) 342, 355, 881, 1209, 1226, 1361
HpaI GTTAAC 1 cut(s) 163
HpaII CCGG 2 cut(s) 34, 1155
HphI GGTGA 2 cut(s) 485, 632
Hpy166II GTNNAC 3 cut(s) 25, 163, 1276
Hpy188I TCNGA 5 cut(s) 347, 387, 516, 676, 907
Hpy188III TCNNGA 9 cut(s) 9, 110, 248, 596, 851, 1043, 1122, 1299, 1358
Hpy8I GTNNAC 3 cut(s) 25, 163, 1276
Hpy99I CGWCG 1 cut(s) 1407
HpyAV CCTTC 3 cut(s) 384, 738, 802
HpyCH4III ACNGT 1 cut(s) 493
HpyCH4IV ACGT 1 cut(s) 1402
HpyCH4V TGCA 5 cut(s) 170, 214, 637, 989, 1037
HpyF10VI GCNNNNNNNGC 6 cut(s) 155, 211, 288, 800, 809, 1268
HpyF3I CTNAG 4 cut(s) 55, 351, 1044, 1313
HpySE526I ACGT 1 cut(s) 1402
Ksp22I TGATCA 1 cut(s) 511
KspAI GTTAAC 1 cut(s) 163
Kzo9I GATC 5 cut(s) 112, 399, 511, 1303, 1386
LmnI GCTCC 3 cut(s) 997, 1045, 1097
Lsp1109I GCAGC 7 cut(s) 253, 517, 539, 621, 790, 1001, 1049
LweI GCATC 1 cut(s) 646
MaeI CTAG 2 cut(s) 339, 815
MaeII ACGT 1 cut(s) 1402
MaeIII GTNAC 3 cut(s) 362, 851, 1367
MalI GATC 5 cut(s) 114, 401, 513, 1305, 1388
MboI GATC 5 cut(s) 112, 399, 511, 1303, 1386
MboII GAAGA 4 cut(s) 24, 270, 799, 815
MflI RGATCY 3 cut(s) 399, 1303, 1386
MhlI GDGCHC 5 cut(s) 308, 714, 977, 1025, 1264
MlsI TGGCCA 2 cut(s) 920, 1256
MluCI AATT 8 cut(s) 171, 517, 615, 704, 936, 1112, 1166, 1218
MluNI TGGCCA 2 cut(s) 920, 1256
MlyI GAGTC 2 cut(s) 364, 1218
MmeI TCCRAC 1 cut(s) 858
MnlI CCTC 9 cut(s) 126, 260, 385, 393, 469, 561, 592, 670, 1223
Mox20I TGGCCA 2 cut(s) 920, 1256
Mph1103I ATGCAT 1 cut(s) 216
MroXI GAANNNNTTC 2 cut(s) 690, 748
MscI TGGCCA 2 cut(s) 920, 1256
MslI CAYNNNNRTG 5 cut(s) 219, 769, 951, 982, 1032
Msp20I TGGCCA 2 cut(s) 920, 1256
MspA1I CMGCKG 2 cut(s) 530, 634
MspI CCGG 2 cut(s) 34, 1155
MspR9I CCNGG 6 cut(s) 255, 445, 540, 951, 1155, 1156
Mva1269I GAATGC 1 cut(s) 282
MvaI CCWGG 4 cut(s) 255, 445, 540, 951
MwoI GCNNNNNNNGC 6 cut(s) 155, 211, 288, 800, 809, 1268
NciI CCSGG 2 cut(s) 1155, 1156
NcoI CCATGG 1 cut(s) 921
NdeI CATATG 1 cut(s) 979
NdeII GATC 5 cut(s) 112, 399, 511, 1303, 1386
NlaIV GGNNCC 3 cut(s) 252, 955, 1438
NmuCI GTSAC 3 cut(s) 362, 851, 1367
NsiI ATGCAT 1 cut(s) 216
OliI CACNNNNGTG 1 cut(s) 951
PctI GAATGC 1 cut(s) 282
PdmI GAANNNNTTC 2 cut(s) 690, 748
PfeI GAWTC 4 cut(s) 342, 881, 1226, 1361
PkrI GCNGC 7 cut(s) 243, 529, 532, 636, 805, 991, 1039
PleI GAGTC 2 cut(s) 363, 1217
PpsI GAGTC 2 cut(s) 363, 1217
PshBI ATTAAT 1 cut(s) 935
Psp124BI GAGCTC 1 cut(s) 1264
Psp6I CCWGG 4 cut(s) 253, 443, 538, 949
PspGI CCWGG 4 cut(s) 253, 443, 538, 949
PspN4I GGNNCC 3 cut(s) 252, 955, 1438
PspPI GGNCC 3 cut(s) 66, 1179, 1436
PstNI CAGNNNCTG 1 cut(s) 1043
PsuI RGATCY 3 cut(s) 399, 1303, 1386
PvuII CAGCTG 2 cut(s) 530, 634
RsaI GTAC 2 cut(s) 134, 490
RsaNI GTAC 2 cut(s) 133, 489
RseI CAYNNNNRTG 5 cut(s) 219, 769, 951, 982, 1032
SacI GAGCTC 1 cut(s) 1264
SatI GCNGC 7 cut(s) 242, 528, 531, 635, 804, 990, 1038
Sau3AI GATC 5 cut(s) 112, 399, 511, 1303, 1386
Sau96I GGNCC 3 cut(s) 66, 1179, 1436
ScaI AGTACT 1 cut(s) 134
SchI GAGTC 2 cut(s) 364, 1218
ScrFI CCNGG 6 cut(s) 255, 445, 540, 951, 1155, 1156
SduI GDGCHC 5 cut(s) 308, 714, 977, 1025, 1264
SfaNI GCATC 1 cut(s) 646
SfcI CTRYAG 3 cut(s) 39, 144, 1056
SinI GGWCC 1 cut(s) 1436
SmaI CCCGGG 1 cut(s) 1156
SmiMI CAYNNNNRTG 5 cut(s) 219, 769, 951, 982, 1032
Sse9I AATT 8 cut(s) 171, 517, 615, 704, 936, 1112, 1166, 1218
SsiI CCGC 1 cut(s) 438
SspMI CTAG 2 cut(s) 339, 815
SstI GAGCTC 1 cut(s) 1264
StyD4I CCNGG 6 cut(s) 253, 443, 538, 949, 1153, 1154
StyI CCWWGG 3 cut(s) 625, 796, 921
TaaI ACNGT 1 cut(s) 493
TaiI ACGT 1 cut(s) 1405
TaqI TCGA 2 cut(s) 1110, 1298
TasI AATT 8 cut(s) 171, 517, 615, 704, 936, 1112, 1166, 1218
TatI WGTACW 1 cut(s) 132
TfiI GAWTC 4 cut(s) 342, 881, 1226, 1361
TscAI CASTG 1 cut(s) 1372
TseFI GTSAC 3 cut(s) 362, 851, 1367
TseI GCWGC 7 cut(s) 241, 527, 530, 634, 803, 989, 1037
Tsp45I GTSAC 3 cut(s) 362, 851, 1367
TspDTI ATGAA 8 cut(s) 231, 441, 510, 717, 759, 800, 846, 1340
TspGWI ACGGA 1 cut(s) 348
TspMI CCCGGG 1 cut(s) 1154
TspRI CASTG 1 cut(s) 1372
VpaK11BI GGWCC 1 cut(s) 1436
VspI ATTAAT 1 cut(s) 935
XagI CCTNNNNNAGG 1 cut(s) 543
XapI RAATTY 4 cut(s) 517, 615, 704, 1218
XcmI CCANNNNNNNNNTGG 1 cut(s) 1415
XmaI CCCGGG 1 cut(s) 1154
XmnI GAANNNNTTC 2 cut(s) 690, 748
XspI CTAG 2 cut(s) 339, 815
ZrmI AGTACT 1 cut(s) 134
Zsp2I ATGCAT 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.