Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 146 |
| Acc36I | ACCTGC | 1 cut(s) | 146 |
| AccII | CGCG | 1 cut(s) | 684 |
| AciI | CCGC | 3 cut(s) | 206, 240, 684 |
| AcoI | YGGCCR | 2 cut(s) | 222, 409 |
| AcuI | CTGAAG | 3 cut(s) | 411, 509, 555 |
| AfaI | GTAC | 1 cut(s) | 416 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 483 |
| AjiI | CACGTC | 2 cut(s) | 172, 299 |
| AjnI | CCWGG | 1 cut(s) | 550 |
| AluBI | AGCT | 3 cut(s) | 336, 431, 523 |
| AluI | AGCT | 3 cut(s) | 336, 431, 523 |
| Alw26I | GTCTC | 1 cut(s) | 178 |
| AoxI | GGCC | 5 cut(s) | 222, 348, 409, 485, 668 |
| ApeKI | GCWGC | 3 cut(s) | 20, 520, 677 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 655 |
| AspS9I | GGNCC | 5 cut(s) | 149, 348, 548, 554, 605 |
| AsuHPI | GGTGA | 1 cut(s) | 170 |
| AvaII | GGWCC | 4 cut(s) | 149, 548, 554, 605 |
| BalI | TGGCCA | 2 cut(s) | 224, 411 |
| BbsI | GAAGAC | 2 cut(s) | 301, 543 |
| BbvI | GCAGC | 3 cut(s) | 7, 532, 664 |
| BccI | CCATC | 3 cut(s) | 140, 401, 679 |
| BciT130I | CCWGG | 1 cut(s) | 552 |
| BcoDI | GTCTC | 1 cut(s) | 178 |
| BfaI | CTAG | 1 cut(s) | 32 |
| BfmI | CTRYAG | 1 cut(s) | 321 |
| BfuAI | ACCTGC | 1 cut(s) | 146 |
| BisI | GCNGC | 4 cut(s) | 21, 207, 521, 678 |
| BlsI | GCNGC | 4 cut(s) | 22, 208, 522, 679 |
| BmcAI | AGTACT | 1 cut(s) | 416 |
| Bme1390I | CCNGG | 1 cut(s) | 552 |
| Bme18I | GGWCC | 4 cut(s) | 149, 548, 554, 605 |
| BmgBI | CACGTC | 2 cut(s) | 172, 299 |
| BmgT120I | GGNCC | 5 cut(s) | 149, 348, 548, 554, 605 |
| BmiI | GGNNCC | 6 cut(s) | 210, 531, 549, 556, 607, 637 |
| BmrFI | CCNGG | 1 cut(s) | 552 |
| BmsI | GCATC | 1 cut(s) | 449 |
| BpiI | GAAGAC | 2 cut(s) | 301, 543 |
| Bpu10I | CCTNAGC | 1 cut(s) | 24 |
| BsaBI | GATNNNNATC | 1 cut(s) | 463 |
| BsaJI | CCNNGG | 2 cut(s) | 13, 141 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 483 |
| Bse1I | ACTGG | 2 cut(s) | 412, 591 |
| Bse3DI | GCAATG | 1 cut(s) | 649 |
| Bse8I | GATNNNNATC | 1 cut(s) | 463 |
| BseBI | CCWGG | 1 cut(s) | 552 |
| BseDI | CCNNGG | 2 cut(s) | 13, 141 |
| BseGI | GGATG | 1 cut(s) | 151 |
| BseJI | GATNNNNATC | 1 cut(s) | 463 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 483 |
| BseMI | GCAATG | 1 cut(s) | 649 |
| BseMII | CTCAG | 1 cut(s) | 204 |
| BseNI | ACTGG | 2 cut(s) | 412, 591 |
| BseXI | GCAGC | 3 cut(s) | 7, 532, 664 |
| BsgI | GTGCAG | 1 cut(s) | 663 |
| Bsh1236I | CGCG | 1 cut(s) | 684 |
| BshFI | GGCC | 5 cut(s) | 224, 350, 411, 487, 670 |
| BslFI | GGGAC | 3 cut(s) | 86, 205, 591 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 483 |
| BsmAI | GTCTC | 1 cut(s) | 178 |
| BsmBI | CGTCTC | 1 cut(s) | 178 |
| BsmFI | GGGAC | 3 cut(s) | 86, 205, 591 |
| BsnI | GGCC | 5 cut(s) | 224, 350, 411, 487, 670 |
| Bsp143I | GATC | 1 cut(s) | 63 |
| BspACI | CCGC | 3 cut(s) | 206, 240, 684 |
| BspANI | GGCC | 5 cut(s) | 224, 350, 411, 487, 670 |
| BspCNI | CTCAG | 1 cut(s) | 205 |
| BspFNI | CGCG | 1 cut(s) | 684 |
| BspHI | TCATGA | 1 cut(s) | 492 |
| BspLI | GGNNCC | 6 cut(s) | 210, 531, 549, 556, 607, 637 |
| BspMI | ACCTGC | 1 cut(s) | 146 |
| BsrDI | GCAATG | 1 cut(s) | 649 |
| BsrI | ACTGG | 2 cut(s) | 412, 591 |
| BssECI | CCNNGG | 2 cut(s) | 13, 141 |
| BssMI | GATC | 1 cut(s) | 63 |
| BssT1I | CCWWGG | 2 cut(s) | 13, 141 |
| Bst2UI | CCWGG | 1 cut(s) | 552 |
| Bst4CI | ACNGT | 2 cut(s) | 47, 317 |
| Bst6I | CTCTTC | 1 cut(s) | 666 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 653 |
| BstC8I | GCNNGC | 2 cut(s) | 367, 682 |
| BstDEI | CTNAG | 3 cut(s) | 24, 213, 337 |
| BstF5I | GGATG | 1 cut(s) | 151 |
| BstFNI | CGCG | 1 cut(s) | 684 |
| BstKTI | GATC | 1 cut(s) | 66 |
| BstMAI | GTCTC | 1 cut(s) | 178 |
| BstMBI | GATC | 1 cut(s) | 63 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 17, 113, 437, 529, 653 |
| BstNI | CCWGG | 1 cut(s) | 552 |
| BstSCI | CCNGG | 1 cut(s) | 550 |
| BstSFI | CTRYAG | 1 cut(s) | 321 |
| BstUI | CGCG | 1 cut(s) | 684 |
| BstV1I | GCAGC | 3 cut(s) | 7, 532, 664 |
| BstV2I | GAAGAC | 2 cut(s) | 301, 543 |
| BsuRI | GGCC | 5 cut(s) | 224, 350, 411, 487, 670 |
| BtrI | CACGTC | 2 cut(s) | 172, 299 |
| BtsCI | GGATG | 1 cut(s) | 151 |
| BtsIMutI | CAGTG | 2 cut(s) | 52, 598 |
| BveI | ACCTGC | 1 cut(s) | 146 |
| Cac8I | GCNNGC | 2 cut(s) | 367, 682 |
| CciI | TCATGA | 1 cut(s) | 492 |
| Cfr13I | GGNCC | 5 cut(s) | 149, 348, 548, 554, 605 |
| CseI | GACGC | 1 cut(s) | 404 |
| Csp6I | GTAC | 1 cut(s) | 415 |
| CviAII | CATG | 4 cut(s) | 187, 493, 541, 587 |
| CviQI | GTAC | 1 cut(s) | 415 |
| DdeI | CTNAG | 3 cut(s) | 24, 213, 337 |
| DpnI | GATC | 1 cut(s) | 65 |
| DpnII | GATC | 1 cut(s) | 63 |
| DraI | TTTAAA | 1 cut(s) | 79 |
| EaeI | YGGCCR | 2 cut(s) | 222, 409 |
| Eam1104I | CTCTTC | 1 cut(s) | 666 |
| EarI | CTCTTC | 1 cut(s) | 666 |
| Eco130I | CCWWGG | 2 cut(s) | 13, 141 |
| Eco47I | GGWCC | 4 cut(s) | 149, 548, 554, 605 |
| Eco57I | CTGAAG | 3 cut(s) | 411, 509, 555 |
| EcoO109I | RGGNCCY | 1 cut(s) | 554 |
| EcoRII | CCWGG | 1 cut(s) | 550 |
| EcoT14I | CCWWGG | 2 cut(s) | 13, 141 |
| ErhI | CCWWGG | 2 cut(s) | 13, 141 |
| Esp3I | CGTCTC | 1 cut(s) | 178 |
| FaeI | CATG | 4 cut(s) | 190, 496, 544, 590 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 640, 672 |
| FaqI | GGGAC | 3 cut(s) | 86, 205, 591 |
| FatI | CATG | 4 cut(s) | 186, 492, 540, 586 |
| FauNDI | CATATG | 1 cut(s) | 196 |
| Fnu4HI | GCNGC | 4 cut(s) | 21, 207, 521, 678 |
| FokI | GGATG | 1 cut(s) | 158 |
| Fsp4HI | GCNGC | 4 cut(s) | 21, 207, 521, 678 |
| FspBI | CTAG | 1 cut(s) | 32 |
| GluI | GCNGC | 4 cut(s) | 21, 207, 521, 678 |
| HaeIII | GGCC | 5 cut(s) | 224, 350, 411, 487, 670 |
| HgaI | GACGC | 1 cut(s) | 404 |
| Hin1II | CATG | 4 cut(s) | 190, 496, 544, 590 |
| HincII | GTYRAC | 1 cut(s) | 445 |
| HindII | GTYRAC | 1 cut(s) | 445 |
| HinfI | GANTC | 1 cut(s) | 101 |
| HpaI | GTTAAC | 1 cut(s) | 445 |
| HphI | GGTGA | 1 cut(s) | 170 |
| Hpy166II | GTNNAC | 2 cut(s) | 307, 445 |
| Hpy188I | TCNGA | 1 cut(s) | 127 |
| Hpy188III | TCNNGA | 5 cut(s) | 71, 212, 291, 493, 527 |
| Hpy8I | GTNNAC | 2 cut(s) | 307, 445 |
| HpyAV | CCTTC | 1 cut(s) | 612 |
| HpyCH4III | ACNGT | 2 cut(s) | 47, 317 |
| HpyCH4IV | ACGT | 2 cut(s) | 171, 298 |
| HpyCH4V | TGCA | 4 cut(s) | 452, 596, 647, 680 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 17, 113, 437, 529, 653 |
| HpyF3I | CTNAG | 3 cut(s) | 24, 213, 337 |
| HpySE526I | ACGT | 2 cut(s) | 171, 298 |
| Hsp92II | CATG | 4 cut(s) | 190, 496, 544, 590 |
| KspAI | GTTAAC | 1 cut(s) | 445 |
| Kzo9I | GATC | 1 cut(s) | 63 |
| LmnI | GCTCC | 2 cut(s) | 214, 529 |
| Lsp1109I | GCAGC | 3 cut(s) | 7, 532, 664 |
| LweI | GCATC | 1 cut(s) | 449 |
| MaeI | CTAG | 1 cut(s) | 32 |
| MaeII | ACGT | 2 cut(s) | 171, 298 |
| MalI | GATC | 1 cut(s) | 65 |
| MboI | GATC | 1 cut(s) | 63 |
| MboII | GAAGA | 6 cut(s) | 16, 32, 306, 548, 575, 653 |
| MlsI | TGGCCA | 2 cut(s) | 224, 411 |
| MluCI | AATT | 1 cut(s) | 453 |
| MluNI | TGGCCA | 2 cut(s) | 224, 411 |
| MmeI | TCCRAC | 1 cut(s) | 78 |
| MnlI | CCTC | 4 cut(s) | 46, 538, 568, 582 |
| Mox20I | TGGCCA | 2 cut(s) | 224, 411 |
| MroXI | GAANNNNTTC | 1 cut(s) | 655 |
| MscI | TGGCCA | 2 cut(s) | 224, 411 |
| MseI | TTAA | 4 cut(s) | 78, 236, 435, 444 |
| MslI | CAYNNNNRTG | 2 cut(s) | 48, 591 |
| Msp20I | TGGCCA | 2 cut(s) | 224, 411 |
| MspR9I | CCNGG | 1 cut(s) | 552 |
| MvaI | CCWGG | 1 cut(s) | 552 |
| MvnI | CGCG | 1 cut(s) | 684 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 17, 113, 437, 529, 653 |
| NdeI | CATATG | 1 cut(s) | 196 |
| NdeII | GATC | 1 cut(s) | 63 |
| NlaIII | CATG | 4 cut(s) | 190, 496, 544, 590 |
| NlaIV | GGNNCC | 6 cut(s) | 210, 531, 549, 556, 607, 637 |
| PagI | TCATGA | 1 cut(s) | 492 |
| PaqCI | CACCTGC | 1 cut(s) | 146 |
| PdmI | GAANNNNTTC | 1 cut(s) | 655 |
| PfeI | GAWTC | 1 cut(s) | 101 |
| PfoI | TCCNGGA | 1 cut(s) | 550 |
| PkrI | GCNGC | 4 cut(s) | 22, 208, 522, 679 |
| PpuMI | RGGWCCY | 1 cut(s) | 554 |
| Psp5II | RGGWCCY | 1 cut(s) | 554 |
| Psp6I | CCWGG | 1 cut(s) | 550 |
| PspGI | CCWGG | 1 cut(s) | 550 |
| PspN4I | GGNNCC | 6 cut(s) | 210, 531, 549, 556, 607, 637 |
| PspPI | GGNCC | 5 cut(s) | 149, 348, 548, 554, 605 |
| PspPPI | RGGWCCY | 1 cut(s) | 554 |
| RsaI | GTAC | 1 cut(s) | 416 |
| RsaNI | GTAC | 1 cut(s) | 415 |
| RseI | CAYNNNNRTG | 2 cut(s) | 48, 591 |
| SaqAI | TTAA | 4 cut(s) | 78, 236, 435, 444 |
| SatI | GCNGC | 4 cut(s) | 21, 207, 521, 678 |
| Sau3AI | GATC | 1 cut(s) | 63 |
| Sau96I | GGNCC | 5 cut(s) | 149, 348, 548, 554, 605 |
| ScaI | AGTACT | 1 cut(s) | 416 |
| ScrFI | CCNGG | 1 cut(s) | 552 |
| SfaNI | GCATC | 1 cut(s) | 449 |
| SfcI | CTRYAG | 1 cut(s) | 321 |
| SinI | GGWCC | 4 cut(s) | 149, 548, 554, 605 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 48, 591 |
| Sse9I | AATT | 1 cut(s) | 453 |
| SsiI | CCGC | 3 cut(s) | 206, 240, 684 |
| SspMI | CTAG | 1 cut(s) | 32 |
| StyD4I | CCNGG | 1 cut(s) | 550 |
| StyI | CCWWGG | 2 cut(s) | 13, 141 |
| TaaI | ACNGT | 2 cut(s) | 47, 317 |
| TaiI | ACGT | 2 cut(s) | 174, 301 |
| TaqI | TCGA | 3 cut(s) | 279, 457, 616 |
| TasI | AATT | 1 cut(s) | 453 |
| TatI | WGTACW | 1 cut(s) | 414 |
| TauI | GCSGC | 1 cut(s) | 209 |
| TfiI | GAWTC | 1 cut(s) | 101 |
| Tru1I | TTAA | 4 cut(s) | 78, 236, 435, 444 |
| Tru9I | TTAA | 4 cut(s) | 78, 236, 435, 444 |
| TscAI | CASTG | 2 cut(s) | 52, 598 |
| TseI | GCWGC | 3 cut(s) | 20, 520, 677 |
| TspDTI | ATGAA | 5 cut(s) | 17, 509, 575, 582, 644 |
| TspGWI | ACGGA | 1 cut(s) | 648 |
| TspRI | CASTG | 2 cut(s) | 52, 598 |
| VpaK11BI | GGWCC | 4 cut(s) | 149, 548, 554, 605 |
| XmnI | GAANNNNTTC | 1 cut(s) | 655 |
| XspI | CTAG | 1 cut(s) | 32 |
| ZrmI | AGTACT | 1 cut(s) | 416 |