Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclI | AACGTT | 1 cut(s) | 298 |
| AclWI | GGATC | 2 cut(s) | 194, 290 |
| AcoI | YGGCCR | 2 cut(s) | 44, 150 |
| AcsI | RAATTY | 1 cut(s) | 114 |
| AcuI | CTGAAG | 1 cut(s) | 236 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 210 |
| AluBI | AGCT | 3 cut(s) | 158, 208, 250 |
| AluI | AGCT | 3 cut(s) | 158, 208, 250 |
| Alw21I | GWGCWC | 1 cut(s) | 160 |
| AlwI | GGATC | 2 cut(s) | 194, 290 |
| Ama87I | CYCGRG | 1 cut(s) | 50 |
| AoxI | GGCC | 5 cut(s) | 44, 75, 136, 150, 212 |
| ApoI | RAATTY | 1 cut(s) | 114 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 90, 122 |
| AspS9I | GGNCC | 2 cut(s) | 75, 332 |
| AsuC2I | CCSGG | 2 cut(s) | 51, 52 |
| AvaI | CYCGRG | 1 cut(s) | 50 |
| AvaII | GGWCC | 1 cut(s) | 332 |
| BalI | TGGCCA | 1 cut(s) | 152 |
| BanII | GRGCYC | 1 cut(s) | 160 |
| Bbv12I | GWGCWC | 1 cut(s) | 160 |
| BccI | CCATC | 1 cut(s) | 266 |
| BcnI | CCSGG | 2 cut(s) | 51, 52 |
| Bme1390I | CCNGG | 2 cut(s) | 51, 52 |
| Bme18I | GGWCC | 1 cut(s) | 332 |
| BmeT110I | CYCGRG | 1 cut(s) | 50 |
| BmgT120I | GGNCC | 2 cut(s) | 75, 332 |
| BmiI | GGNNCC | 2 cut(s) | 41, 334 |
| BmrFI | CCNGG | 2 cut(s) | 51, 52 |
| Bpu10I | CCTNAGC | 1 cut(s) | 209 |
| BpuMI | CCSGG | 2 cut(s) | 51, 52 |
| BsaJI | CCNNGG | 2 cut(s) | 50, 51 |
| BsaWI | WCCGGW | 1 cut(s) | 36 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 210 |
| Bse118I | RCCGGY | 1 cut(s) | 42 |
| Bse1I | ACTGG | 1 cut(s) | 261 |
| BseDI | CCNNGG | 2 cut(s) | 50, 51 |
| BseGI | GGATG | 1 cut(s) | 277 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 210 |
| BseNI | ACTGG | 1 cut(s) | 261 |
| BshFI | GGCC | 5 cut(s) | 46, 77, 138, 152, 214 |
| BsiHKAI | GWGCWC | 1 cut(s) | 160 |
| BsiHKCI | CYCGRG | 1 cut(s) | 50 |
| BsiSI | CCGG | 3 cut(s) | 37, 43, 51 |
| BslFI | GGGAC | 1 cut(s) | 318 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 210 |
| BsmFI | GGGAC | 1 cut(s) | 318 |
| BsnI | GGCC | 5 cut(s) | 46, 77, 138, 152, 214 |
| BsoBI | CYCGRG | 1 cut(s) | 50 |
| Bsp1286I | GDGCHC | 1 cut(s) | 160 |
| Bsp143I | GATC | 2 cut(s) | 199, 282 |
| BspANI | GGCC | 5 cut(s) | 46, 77, 138, 152, 214 |
| BspLI | GGNNCC | 2 cut(s) | 41, 334 |
| BspPI | GGATC | 2 cut(s) | 194, 290 |
| BsrFI | RCCGGY | 1 cut(s) | 42 |
| BsrI | ACTGG | 1 cut(s) | 261 |
| BssAI | RCCGGY | 1 cut(s) | 42 |
| BssECI | CCNNGG | 2 cut(s) | 50, 51 |
| BssMI | GATC | 2 cut(s) | 199, 282 |
| BstC8I | GCNNGC | 1 cut(s) | 44 |
| BstDEI | CTNAG | 1 cut(s) | 209 |
| BstF5I | GGATG | 1 cut(s) | 277 |
| BstKTI | GATC | 2 cut(s) | 202, 285 |
| BstMBI | GATC | 2 cut(s) | 199, 282 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 164 |
| BstSCI | CCNGG | 2 cut(s) | 49, 50 |
| BstX2I | RGATCY | 2 cut(s) | 199, 282 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 268 |
| BstYI | RGATCY | 2 cut(s) | 199, 282 |
| BsuRI | GGCC | 5 cut(s) | 46, 77, 138, 152, 214 |
| BtsCI | GGATG | 1 cut(s) | 277 |
| BtsIMutI | CAGTG | 1 cut(s) | 268 |
| Cac8I | GCNNGC | 1 cut(s) | 44 |
| Cfr10I | RCCGGY | 1 cut(s) | 42 |
| Cfr13I | GGNCC | 2 cut(s) | 75, 332 |
| Cfr9I | CCCGGG | 1 cut(s) | 50 |
| CviAII | CATG | 3 cut(s) | 220, 268, 314 |
| DdeI | CTNAG | 1 cut(s) | 209 |
| DpnI | GATC | 2 cut(s) | 201, 284 |
| DpnII | GATC | 2 cut(s) | 199, 282 |
| EaeI | YGGCCR | 2 cut(s) | 44, 150 |
| Ecl136II | GAGCTC | 1 cut(s) | 158 |
| Eco147I | AGGCCT | 1 cut(s) | 138 |
| Eco24I | GRGCYC | 1 cut(s) | 160 |
| Eco47I | GGWCC | 1 cut(s) | 332 |
| Eco53kI | GAGCTC | 1 cut(s) | 158 |
| Eco57I | CTGAAG | 1 cut(s) | 236 |
| Eco88I | CYCGRG | 1 cut(s) | 50 |
| EcoICRI | GAGCTC | 1 cut(s) | 158 |
| EcoT38I | GRGCYC | 1 cut(s) | 160 |
| FaeI | CATG | 3 cut(s) | 223, 271, 317 |
| FaiI | YATR | 4 cut(s) | 129, 221, 269, 315 |
| FaqI | GGGAC | 1 cut(s) | 318 |
| FatI | CATG | 3 cut(s) | 219, 267, 313 |
| FokI | GGATG | 1 cut(s) | 284 |
| FriOI | GRGCYC | 1 cut(s) | 160 |
| HaeIII | GGCC | 5 cut(s) | 46, 77, 138, 152, 214 |
| HapII | CCGG | 3 cut(s) | 37, 43, 51 |
| Hin1II | CATG | 3 cut(s) | 223, 271, 317 |
| HincII | GTYRAC | 1 cut(s) | 172 |
| HindII | GTYRAC | 1 cut(s) | 172 |
| HindIII | AAGCTT | 2 cut(s) | 206, 248 |
| HinfI | GANTC | 3 cut(s) | 105, 122, 257 |
| HpaII | CCGG | 3 cut(s) | 37, 43, 51 |
| Hpy166II | GTNNAC | 1 cut(s) | 172 |
| Hpy188III | TCNNGA | 3 cut(s) | 18, 195, 254 |
| Hpy8I | GTNNAC | 1 cut(s) | 172 |
| HpyCH4IV | ACGT | 1 cut(s) | 298 |
| HpyCH4V | TGCA | 1 cut(s) | 179 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 164 |
| HpyF3I | CTNAG | 1 cut(s) | 209 |
| HpySE526I | ACGT | 1 cut(s) | 298 |
| Hsp92II | CATG | 3 cut(s) | 223, 271, 317 |
| KroI | GCCGGC | 1 cut(s) | 42 |
| KroNI | GCCGGC | 1 cut(s) | 44 |
| Kzo9I | GATC | 2 cut(s) | 199, 282 |
| LmnI | GCTCC | 1 cut(s) | 39 |
| LpnPI | CCDG | 8 cut(s) | 3, 50, 56, 64, 152, 166, 239, 274 |
| MaeII | ACGT | 1 cut(s) | 298 |
| MaeIII | GTNAC | 1 cut(s) | 263 |
| MalI | GATC | 2 cut(s) | 201, 284 |
| MboI | GATC | 2 cut(s) | 199, 282 |
| MflI | RGATCY | 2 cut(s) | 199, 282 |
| MhlI | GDGCHC | 1 cut(s) | 160 |
| MlsI | TGGCCA | 1 cut(s) | 152 |
| MluCI | AATT | 2 cut(s) | 8, 114 |
| MluNI | TGGCCA | 1 cut(s) | 152 |
| MlyI | GAGTC | 1 cut(s) | 114 |
| MnlI | CCTC | 1 cut(s) | 119 |
| Mox20I | TGGCCA | 1 cut(s) | 152 |
| MroNI | GCCGGC | 1 cut(s) | 42 |
| MscI | TGGCCA | 1 cut(s) | 152 |
| MseI | TTAA | 4 cut(s) | 69, 132, 162, 323 |
| Msp20I | TGGCCA | 1 cut(s) | 152 |
| MspI | CCGG | 3 cut(s) | 37, 43, 51 |
| MspR9I | CCNGG | 2 cut(s) | 51, 52 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 164 |
| NaeI | GCCGGC | 1 cut(s) | 44 |
| NciI | CCSGG | 2 cut(s) | 51, 52 |
| NdeII | GATC | 2 cut(s) | 199, 282 |
| NgoMIV | GCCGGC | 1 cut(s) | 42 |
| NlaIII | CATG | 3 cut(s) | 223, 271, 317 |
| NlaIV | GGNNCC | 2 cut(s) | 41, 334 |
| NmuCI | GTSAC | 1 cut(s) | 263 |
| PceI | AGGCCT | 1 cut(s) | 138 |
| PdiI | GCCGGC | 1 cut(s) | 44 |
| PfeI | GAWTC | 2 cut(s) | 122, 257 |
| PleI | GAGTC | 1 cut(s) | 113 |
| PpsI | GAGTC | 1 cut(s) | 113 |
| Psp124BI | GAGCTC | 1 cut(s) | 160 |
| Psp1406I | AACGTT | 1 cut(s) | 298 |
| PspN4I | GGNNCC | 2 cut(s) | 41, 334 |
| PspPI | GGNCC | 2 cut(s) | 75, 332 |
| PsuI | RGATCY | 2 cut(s) | 199, 282 |
| SacI | GAGCTC | 1 cut(s) | 160 |
| SaqAI | TTAA | 4 cut(s) | 69, 132, 162, 323 |
| Sau3AI | GATC | 2 cut(s) | 199, 282 |
| Sau96I | GGNCC | 2 cut(s) | 75, 332 |
| SchI | GAGTC | 1 cut(s) | 114 |
| ScrFI | CCNGG | 2 cut(s) | 51, 52 |
| SduI | GDGCHC | 1 cut(s) | 160 |
| SetI | ASST | 6 cut(s) | 85, 160, 177, 210, 252, 301 |
| SinI | GGWCC | 1 cut(s) | 332 |
| SmaI | CCCGGG | 1 cut(s) | 52 |
| Sse9I | AATT | 2 cut(s) | 8, 114 |
| SseBI | AGGCCT | 1 cut(s) | 138 |
| SstI | GAGCTC | 1 cut(s) | 160 |
| StuI | AGGCCT | 1 cut(s) | 138 |
| StyD4I | CCNGG | 2 cut(s) | 49, 50 |
| TaiI | ACGT | 1 cut(s) | 301 |
| TaqI | TCGA | 1 cut(s) | 6 |
| TasI | AATT | 2 cut(s) | 8, 114 |
| TfiI | GAWTC | 2 cut(s) | 122, 257 |
| Tru1I | TTAA | 4 cut(s) | 69, 132, 162, 323 |
| Tru9I | TTAA | 4 cut(s) | 69, 132, 162, 323 |
| TscAI | CASTG | 1 cut(s) | 268 |
| TseFI | GTSAC | 1 cut(s) | 263 |
| Tsp45I | GTSAC | 1 cut(s) | 263 |
| TspDTI | ATGAA | 1 cut(s) | 236 |
| TspMI | CCCGGG | 1 cut(s) | 50 |
| TspRI | CASTG | 1 cut(s) | 268 |
| VpaK11BI | GGWCC | 1 cut(s) | 332 |
| XapI | RAATTY | 1 cut(s) | 114 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 311 |
| XmaI | CCCGGG | 1 cut(s) | 50 |