Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 21 |
| AcoI | YGGCCR | 2 cut(s) | 3, 190 |
| AcuI | CTGAAG | 3 cut(s) | 192, 290, 336 |
| AcvI | CACGTG | 1 cut(s) | 464 |
| AfaI | GTAC | 1 cut(s) | 197 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 264 |
| AgsI | TTSAA | 1 cut(s) | 378 |
| AluBI | AGCT | 4 cut(s) | 83, 117, 212, 304 |
| AluI | AGCT | 4 cut(s) | 83, 117, 212, 304 |
| AoxI | GGCC | 6 cut(s) | 3, 98, 129, 190, 266, 449 |
| ApeKI | GCWGC | 2 cut(s) | 301, 458 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 436 |
| AspS9I | GGNCC | 4 cut(s) | 129, 329, 335, 386 |
| AsuC2I | CCSGG | 1 cut(s) | 333 |
| AvaII | GGWCC | 3 cut(s) | 329, 335, 386 |
| BalI | TGGCCA | 2 cut(s) | 5, 192 |
| BbrPI | CACGTG | 1 cut(s) | 464 |
| BbsI | GAAGAC | 2 cut(s) | 82, 324 |
| BbvI | GCAGC | 2 cut(s) | 313, 445 |
| BccI | CCATC | 2 cut(s) | 182, 460 |
| BcnI | CCSGG | 1 cut(s) | 333 |
| BfmI | CTRYAG | 1 cut(s) | 102 |
| BisI | GCNGC | 2 cut(s) | 302, 459 |
| BlsI | GCNGC | 2 cut(s) | 303, 460 |
| BmcAI | AGTACT | 1 cut(s) | 197 |
| Bme1390I | CCNGG | 1 cut(s) | 333 |
| Bme18I | GGWCC | 3 cut(s) | 329, 335, 386 |
| BmgT120I | GGNCC | 4 cut(s) | 129, 329, 335, 386 |
| BmiI | GGNNCC | 6 cut(s) | 312, 330, 336, 337, 388, 418 |
| BmrFI | CCNGG | 1 cut(s) | 333 |
| BmsI | GCATC | 1 cut(s) | 230 |
| BpiI | GAAGAC | 2 cut(s) | 82, 324 |
| BpuMI | CCSGG | 1 cut(s) | 333 |
| BsaAI | YACGTR | 1 cut(s) | 464 |
| BsaBI | GATNNNNATC | 1 cut(s) | 244 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 264 |
| Bse118I | RCCGGY | 1 cut(s) | 96 |
| Bse1I | ACTGG | 2 cut(s) | 193, 372 |
| Bse3DI | GCAATG | 1 cut(s) | 430 |
| Bse8I | GATNNNNATC | 1 cut(s) | 244 |
| BseJI | GATNNNNATC | 1 cut(s) | 244 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 264 |
| BseMI | GCAATG | 1 cut(s) | 430 |
| BseNI | ACTGG | 2 cut(s) | 193, 372 |
| BseXI | GCAGC | 2 cut(s) | 313, 445 |
| BsgI | GTGCAG | 1 cut(s) | 444 |
| BshFI | GGCC | 6 cut(s) | 5, 100, 131, 192, 268, 451 |
| BsiSI | CCGG | 2 cut(s) | 97, 332 |
| BslFI | GGGAC | 2 cut(s) | 348, 372 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 264 |
| BsmFI | GGGAC | 2 cut(s) | 348, 372 |
| BsnI | GGCC | 6 cut(s) | 5, 100, 131, 192, 268, 451 |
| BspACI | CCGC | 1 cut(s) | 21 |
| BspANI | GGCC | 6 cut(s) | 5, 100, 131, 192, 268, 451 |
| BspHI | TCATGA | 1 cut(s) | 273 |
| BspLI | GGNNCC | 6 cut(s) | 312, 330, 336, 337, 388, 418 |
| BsrDI | GCAATG | 1 cut(s) | 430 |
| BsrFI | RCCGGY | 1 cut(s) | 96 |
| BsrI | ACTGG | 2 cut(s) | 193, 372 |
| BssAI | RCCGGY | 1 cut(s) | 96 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 434 |
| BstBAI | YACGTR | 1 cut(s) | 464 |
| BstC8I | GCNNGC | 1 cut(s) | 148 |
| BstDEI | CTNAG | 1 cut(s) | 118 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 218, 434 |
| BstSCI | CCNGG | 1 cut(s) | 331 |
| BstSFI | CTRYAG | 1 cut(s) | 102 |
| BstV1I | GCAGC | 2 cut(s) | 313, 445 |
| BstV2I | GAAGAC | 2 cut(s) | 82, 324 |
| BsuRI | GGCC | 6 cut(s) | 5, 100, 131, 192, 268, 451 |
| Cac8I | GCNNGC | 1 cut(s) | 148 |
| CciI | TCATGA | 1 cut(s) | 273 |
| Cfr10I | RCCGGY | 1 cut(s) | 96 |
| Cfr13I | GGNCC | 4 cut(s) | 129, 329, 335, 386 |
| CseI | GACGC | 1 cut(s) | 185 |
| Csp6I | GTAC | 1 cut(s) | 196 |
| CviAII | CATG | 3 cut(s) | 274, 322, 368 |
| CviQI | GTAC | 1 cut(s) | 196 |
| DdeI | CTNAG | 1 cut(s) | 118 |
| EaeI | YGGCCR | 2 cut(s) | 3, 190 |
| Eco47I | GGWCC | 3 cut(s) | 329, 335, 386 |
| Eco57I | CTGAAG | 3 cut(s) | 192, 290, 336 |
| Eco72I | CACGTG | 1 cut(s) | 464 |
| EcoO109I | RGGNCCY | 1 cut(s) | 335 |
| FaeI | CATG | 3 cut(s) | 277, 325, 371 |
| FaiI | YATR | 7 cut(s) | 39, 56, 275, 323, 369, 410, 475 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 421, 453 |
| FaqI | GGGAC | 2 cut(s) | 348, 372 |
| FatI | CATG | 3 cut(s) | 273, 321, 367 |
| Fnu4HI | GCNGC | 2 cut(s) | 302, 459 |
| Fsp4HI | GCNGC | 2 cut(s) | 302, 459 |
| GluI | GCNGC | 2 cut(s) | 302, 459 |
| HaeIII | GGCC | 6 cut(s) | 5, 100, 131, 192, 268, 451 |
| HapII | CCGG | 2 cut(s) | 97, 332 |
| HgaI | GACGC | 1 cut(s) | 185 |
| Hin1II | CATG | 3 cut(s) | 277, 325, 371 |
| HincII | GTYRAC | 1 cut(s) | 226 |
| HindII | GTYRAC | 1 cut(s) | 226 |
| HpaI | GTTAAC | 1 cut(s) | 226 |
| HpaII | CCGG | 2 cut(s) | 97, 332 |
| Hpy166II | GTNNAC | 2 cut(s) | 88, 226 |
| Hpy188III | TCNNGA | 3 cut(s) | 72, 274, 308 |
| Hpy8I | GTNNAC | 2 cut(s) | 88, 226 |
| HpyAV | CCTTC | 1 cut(s) | 393 |
| HpyCH4IV | ACGT | 1 cut(s) | 463 |
| HpyCH4V | TGCA | 3 cut(s) | 233, 428, 461 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 218, 434 |
| HpyF3I | CTNAG | 1 cut(s) | 118 |
| HpySE526I | ACGT | 1 cut(s) | 463 |
| Hsp92II | CATG | 3 cut(s) | 277, 325, 371 |
| KflI | GGGWCCC | 1 cut(s) | 335 |
| KspAI | GTTAAC | 1 cut(s) | 226 |
| Lsp1109I | GCAGC | 2 cut(s) | 313, 445 |
| LweI | GCATC | 1 cut(s) | 230 |
| MaeII | ACGT | 1 cut(s) | 463 |
| MboII | GAAGA | 3 cut(s) | 87, 329, 356 |
| MlsI | TGGCCA | 2 cut(s) | 5, 192 |
| MluCI | AATT | 1 cut(s) | 234 |
| MluNI | TGGCCA | 2 cut(s) | 5, 192 |
| MnlI | CCTC | 3 cut(s) | 319, 349, 363 |
| Mox20I | TGGCCA | 2 cut(s) | 5, 192 |
| MroXI | GAANNNNTTC | 1 cut(s) | 436 |
| MscI | TGGCCA | 2 cut(s) | 5, 192 |
| MseI | TTAA | 3 cut(s) | 17, 216, 225 |
| Msp20I | TGGCCA | 2 cut(s) | 5, 192 |
| MspI | CCGG | 2 cut(s) | 97, 332 |
| MspR9I | CCNGG | 1 cut(s) | 333 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 218, 434 |
| NciI | CCSGG | 1 cut(s) | 333 |
| NlaIII | CATG | 3 cut(s) | 277, 325, 371 |
| NlaIV | GGNNCC | 6 cut(s) | 312, 330, 336, 337, 388, 418 |
| PagI | TCATGA | 1 cut(s) | 273 |
| PdmI | GAANNNNTTC | 1 cut(s) | 436 |
| PfoI | TCCNGGA | 1 cut(s) | 331 |
| PkrI | GCNGC | 2 cut(s) | 303, 460 |
| PmaCI | CACGTG | 1 cut(s) | 464 |
| PmlI | CACGTG | 1 cut(s) | 464 |
| Ppu21I | YACGTR | 1 cut(s) | 464 |
| PpuMI | RGGWCCY | 1 cut(s) | 335 |
| Psp5II | RGGWCCY | 1 cut(s) | 335 |
| PspCI | CACGTG | 1 cut(s) | 464 |
| PspN4I | GGNNCC | 6 cut(s) | 312, 330, 336, 337, 388, 418 |
| PspPI | GGNCC | 4 cut(s) | 129, 329, 335, 386 |
| PspPPI | RGGWCCY | 1 cut(s) | 335 |
| RsaI | GTAC | 1 cut(s) | 197 |
| RsaNI | GTAC | 1 cut(s) | 196 |
| SaqAI | TTAA | 3 cut(s) | 17, 216, 225 |
| SatI | GCNGC | 2 cut(s) | 302, 459 |
| Sau96I | GGNCC | 4 cut(s) | 129, 329, 335, 386 |
| ScaI | AGTACT | 1 cut(s) | 197 |
| ScrFI | CCNGG | 1 cut(s) | 333 |
| SfaNI | GCATC | 1 cut(s) | 230 |
| SfcI | CTRYAG | 1 cut(s) | 102 |
| SinI | GGWCC | 3 cut(s) | 329, 335, 386 |
| Sse9I | AATT | 1 cut(s) | 234 |
| SsiI | CCGC | 1 cut(s) | 21 |
| StyD4I | CCNGG | 1 cut(s) | 331 |
| TaiI | ACGT | 1 cut(s) | 466 |
| TaqI | TCGA | 3 cut(s) | 60, 238, 397 |
| TasI | AATT | 1 cut(s) | 234 |
| TatI | WGTACW | 1 cut(s) | 195 |
| Tru1I | TTAA | 3 cut(s) | 17, 216, 225 |
| Tru9I | TTAA | 3 cut(s) | 17, 216, 225 |
| TseI | GCWGC | 2 cut(s) | 301, 458 |
| TspDTI | ATGAA | 4 cut(s) | 290, 356, 363, 425 |
| TspGWI | ACGGA | 1 cut(s) | 429 |
| VpaK11BI | GGWCC | 3 cut(s) | 329, 335, 386 |
| XmnI | GAANNNNTTC | 1 cut(s) | 436 |
| ZrmI | AGTACT | 1 cut(s) | 197 |