RLG00000027281
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000305
Physical Location & Seq
Reverse (-)
10248129 .. 10250205
2077 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027281

Sequence Viewer

Length: 663 bp
ATGTCATCTGTCTCAGTGTCTTCAACTCCTCGAACAGAGGGTGAAATCTTGATGTCTTCCAACCTGATGAACTTCTTCTTTAATGAACTGAAAACAGCCACAAGGAACTTTCGTCCTGACAGTATGATGGGTGAAGGCGGATTTGGTTGTGTTTTTAAGGGCTGGGTTGATGAGAATGCATTAACAGCTGCGAAGCCTGGTACTGGAATGGTTATTGCTGTGAAGAGGCTTAACCAAGAGGGTCTCCAGGGTCACAAGGAATGGTTGACAGAAACCAACTATCTTGGACAGCTCCGACACCCAAATCTTGTGAAGCTGATTGGTTATTGCTTGGAGGATGACCACCGGCTTCTGGTGTATGAATTCATGCCTAGTGGCAGTGTGGACAATCATCTATTTAGAAGGGCTTCTTACTTTCAACCACTGTCTTGGCCCCTTCATATGAAGATTGCCCTCGGTACTGCTGAGGGTCTAGCATTTCTTCACAGTGATGGGGCAAAAGTGATCTATCAGGACTTGAAAACTTTTAATATCCTGTTGGATTCAGCCTGCAATGCCAAACGCTCTGGTTTTGGTTTAGCCAAGGATGGACCAGCAGGTGATAAAAGCCACGTCTCAACAAGGGTCATGGGGACATATAGGTCATTTAACCGCCAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0035821 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0046777 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0060089 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0075136 GO:0080134 GO:0098542 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

221

Amino Acids

24.5

Weight (kDa)

8.96

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 40 - 209 2.8e-24 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 41 - 204 2.5e-26 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000305)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39660 AT3G55450 AT3G55450
fragaria_vesca FvH4_7g22540 FvH4_7g22540 FvH4_7g22540 FvH4_7g22540 FvH4_7g22550 FvH4_7g22550 FvH4_7g22590 FvH4_7g22592 FvH4_7g22592 FvH4_7g22593 FvH4_7g22593 FvH4_7g22593
malus_domestica MD01G1134700.v1.1 MD07G1199400.v1.1
prunus_persica Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1
pyrus_communis pycom01g15540 pycom07g18630
rosa_chinensis RchiOBHm_Chr1g0367221 RchiOBHm_Chr1g0367231 RchiOBHm_Chr1g0367251 RchiOBHm_Chr1g0367261 RchiOBHm_Chr1g0367311 RchiOBHm_Chr1g0367321 RchiOBHm_Chr1g0367351 RchiOBHm_Chr1g0367401 RchiOBHm_Chr1g0367411 RchiOBHm_Chr1g0367581 RchiOBHm_Chr5g0029531
rosa_laevigata RLG00000001400 RLG00000024338 RLG00000027271 RLG00000027274 RLG00000027277 RLG00000027278 RLG00000027280 RLG00000027281 RLG00000027282 RLG00000027285 RLG00000027286 RLG00000027288 RLG00000033176
rosa_multiflora Rmu_sc0002690.1_g000002 Rmu_sc0004181.1_g000007 Rmu_sc0008470.1_g000013 Rmu_sc0008470.1_g000015 Rmu_sc0009775.1_g000001 Rmu_sc0009775.1_g000003 Rmu_sc0009775.1_g000004 Rmu_sc0009775.1_g000005 Rmu_sc0011000.1_g000008 Rmu_sc0014291.1_g000001 Rmu_sc0014291.1_g000005 Rmu_sc0014291.1_g000008
rosa_roxburghii Rroxscaffold_1G00050440 Rroxscaffold_4G00289340 Rroxscaffold_4G00289350 Rroxscaffold_4G00289380 Rroxscaffold_4G00289400 Rroxscaffold_4G00289410 Rroxscaffold_4G00289450 Rroxscaffold_4G00289490 Rroxscaffold_4G00289500 Rroxscaffold_4G00289510 Rroxscaffold_6G00420130 Rroxscaffold_6G00421370
rosa_rugosa Rorug01G0335600.1 Rorug01G0335700 Rorug01G0335800 Rorug01G0335800 Rorug01G0335800 Rorug01G0335800 Rorug01G0335900 Rorug01G0335900 Rorug01G0336000 Rorug01G0336000 Rorug05G0114700 Rorug05G0114800
rosa_samantha Rh1AG342300 Rh1AG342500 Rh1AG342700 Rh1BG302700 Rh1BG303000 Rh1BG303100 Rh1BG303400 Rh1BG303500 Rh1BG303800 Rh1BG304100 Rh1CG319200 Rh1CG319400 Rh1CG319800 Rh1CG319900 Rh1DG335300 Rh1DG335500 Rh1DG335900 Rh1DG336200 Rh2AG106400 Rh3DG307700 Rh4CG281300 Rh5AG206700 Rh5CG227400 Rh5DG209100 Rh7CG338300
rosa_wichuraiana Rw1G030420 Rw1G030430 Rw1G030460 Rw1G030470 Rw1G030480 Rw1G030510 Rw1G030520 Rw5G018800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 587
AasI GACNNNNNNGTC 1 cut(s) 640
Acc36I ACCTGC 1 cut(s) 587
AciI CCGC 2 cut(s) 138, 652
AcsI RAATTY 1 cut(s) 362
AfaI GTAC 2 cut(s) 202, 460
AfiI CCNNNNNNNGG 2 cut(s) 203, 352
AgsI TTSAA 3 cut(s) 24, 419, 520
AjiI CACGTC 1 cut(s) 613
AjnI CCWGG 2 cut(s) 196, 246
AjuI GAANNNNNNNTTGG 2 cut(s) 126, 158
AluBI AGCT 3 cut(s) 188, 292, 316
AluI AGCT 3 cut(s) 188, 292, 316
Alw26I GTCTC 3 cut(s) 16, 248, 619
AoxI GGCC 1 cut(s) 431
ApeKI GCWGC 1 cut(s) 188
ApoI RAATTY 1 cut(s) 362
Asp700I GAANNNNTTC 2 cut(s) 74, 406
AspS9I GGNCC 2 cut(s) 432, 590
AsuHPI GGTGA 3 cut(s) 53, 143, 611
AvaII GGWCC 1 cut(s) 590
BbsI GAAGAC 2 cut(s) 12, 48
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 1 cut(s) 175
BccI CCATC 3 cut(s) 121, 485, 581
BciT130I CCWGG 2 cut(s) 198, 248
BcoDI GTCTC 3 cut(s) 16, 248, 619
BfaI CTAG 2 cut(s) 372, 473
BfuAI ACCTGC 1 cut(s) 587
BisI GCNGC 1 cut(s) 189
BlsI GCNGC 1 cut(s) 190
Bme1390I CCNGG 2 cut(s) 198, 248
Bme18I GGWCC 1 cut(s) 590
BmgBI CACGTC 1 cut(s) 613
BmgT120I GGNCC 2 cut(s) 432, 590
BmiI GGNNCC 1 cut(s) 434
BmrFI CCNGG 2 cut(s) 198, 248
BpiI GAAGAC 2 cut(s) 12, 48
BpmI CTGGAG 1 cut(s) 230
Bpu10I CCTNAGC 1 cut(s) 465
BsaI GGTCTC 1 cut(s) 248
BsaJI CCNNGG 3 cut(s) 247, 454, 582
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 352
Bse118I RCCGGY 1 cut(s) 345
Bse1I ACTGG 1 cut(s) 208
Bse3DI GCAATG 1 cut(s) 559
BseBI CCWGG 2 cut(s) 198, 248
BseDI CCNNGG 3 cut(s) 247, 454, 582
BseGI GGATG 2 cut(s) 343, 592
BseLI CCNNNNNNNGG 2 cut(s) 203, 352
BseMI GCAATG 1 cut(s) 559
BseMII CTCAG 2 cut(s) 27, 456
BseNI ACTGG 1 cut(s) 208
BseRI GAGGAG 1 cut(s) 18
BseXI GCAGC 1 cut(s) 175
BseYI CCCAGC 1 cut(s) 162
BshFI GGCC 1 cut(s) 433
BsiSI CCGG 1 cut(s) 346
BslFI GGGAC 1 cut(s) 646
BslI CCNNNNNNNGG 2 cut(s) 203, 352
BsmAI GTCTC 3 cut(s) 16, 248, 619
BsmBI CGTCTC 1 cut(s) 619
BsmFI GGGAC 1 cut(s) 646
BsmI GAATGC 1 cut(s) 181
BsnI GGCC 1 cut(s) 433
Bso31I GGTCTC 1 cut(s) 248
Bsp143I GATC 1 cut(s) 504
BspACI CCGC 2 cut(s) 138, 652
BspANI GGCC 1 cut(s) 433
BspCNI CTCAG 2 cut(s) 26, 457
BspLI GGNNCC 1 cut(s) 434
BspMI ACCTGC 1 cut(s) 587
BspTNI GGTCTC 1 cut(s) 248
BsrDI GCAATG 1 cut(s) 559
BsrFI RCCGGY 1 cut(s) 345
BsrI ACTGG 1 cut(s) 208
BssAI RCCGGY 1 cut(s) 345
BssECI CCNNGG 3 cut(s) 247, 454, 582
BssMI GATC 1 cut(s) 504
BssT1I CCWWGG 1 cut(s) 582
Bst2UI CCWGG 2 cut(s) 198, 248
Bst4CI ACNGT 3 cut(s) 122, 426, 488
Bst6I CTCTTC 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 550
BstDEI CTNAG 2 cut(s) 13, 465
BstF5I GGATG 2 cut(s) 343, 592
BstKTI GATC 1 cut(s) 507
BstMAI GTCTC 3 cut(s) 16, 248, 619
BstMBI GATC 1 cut(s) 504
BstMWI GCNNNNNNNGC 2 cut(s) 185, 554
BstNI CCWGG 2 cut(s) 198, 248
BstSCI CCNGG 2 cut(s) 196, 246
BstV1I GCAGC 1 cut(s) 175
BstV2I GAAGAC 2 cut(s) 12, 48
BstXI CCANNNNNNTGG 1 cut(s) 429
BsuRI GGCC 1 cut(s) 433
BtrI CACGTC 1 cut(s) 613
BtsCI GGATG 2 cut(s) 343, 592
BtsI GCAGTG 1 cut(s) 385
BtsIMutI CAGTG 4 cut(s) 21, 385, 422, 493
BveI ACCTGC 1 cut(s) 587
Cac8I GCNNGC 1 cut(s) 550
Cfr10I RCCGGY 1 cut(s) 345
Cfr13I GGNCC 2 cut(s) 432, 590
Csp6I GTAC 2 cut(s) 201, 459
CviAII CATG 2 cut(s) 367, 628
CviQI GTAC 2 cut(s) 201, 459
DdeI CTNAG 2 cut(s) 13, 465
DpnI GATC 1 cut(s) 506
DpnII GATC 1 cut(s) 504
DrdI GACNNNNNNGTC 1 cut(s) 640
DseDI GACNNNNNNGTC 1 cut(s) 640
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
EciI GGCGGA 1 cut(s) 153
Eco130I CCWWGG 1 cut(s) 582
Eco31I GGTCTC 1 cut(s) 248
Eco47I GGWCC 1 cut(s) 590
EcoRI GAATTC 1 cut(s) 362
EcoRII CCWGG 2 cut(s) 196, 246
EcoT14I CCWWGG 1 cut(s) 582
EcoT22I ATGCAT 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 582
Esp3I CGTCTC 1 cut(s) 619
FaeI CATG 2 cut(s) 370, 631
FaiI YATR 8 cut(s) 125, 360, 368, 441, 443, 629, 637, 639
FalI AAGNNNNNCTT 2 cut(s) 394, 426
FaqI GGGAC 1 cut(s) 646
FatI CATG 2 cut(s) 366, 627
FauNDI CATATG 1 cut(s) 441
Fnu4HI GCNGC 1 cut(s) 189
FokI GGATG 2 cut(s) 350, 599
Fsp4HI GCNGC 1 cut(s) 189
FspBI CTAG 2 cut(s) 372, 473
GluI GCNGC 1 cut(s) 189
GsaI CCCAGC 1 cut(s) 166
GsuI CTGGAG 1 cut(s) 230
HaeIII GGCC 1 cut(s) 433
HapII CCGG 1 cut(s) 346
Hin1II CATG 2 cut(s) 370, 631
HincII GTYRAC 1 cut(s) 267
HindII GTYRAC 1 cut(s) 267
HinfI GANTC 1 cut(s) 542
HpaII CCGG 1 cut(s) 346
HphI GGTGA 3 cut(s) 53, 143, 611
Hpy166II GTNNAC 2 cut(s) 267, 385
Hpy188I TCNGA 1 cut(s) 296
Hpy188III TCNNGA 3 cut(s) 49, 116, 512
Hpy8I GTNNAC 2 cut(s) 267, 385
HpyAV CCTTC 3 cut(s) 128, 396, 446
HpyCH4III ACNGT 3 cut(s) 122, 426, 488
HpyCH4IV ACGT 1 cut(s) 612
HpyCH4V TGCA 2 cut(s) 179, 552
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 554
HpyF3I CTNAG 2 cut(s) 13, 465
HpySE526I ACGT 1 cut(s) 612
Hsp92II CATG 2 cut(s) 370, 631
Kzo9I GATC 1 cut(s) 504
LmnI GCTCC 1 cut(s) 297
Lsp1109I GCAGC 1 cut(s) 175
MaeI CTAG 2 cut(s) 372, 473
MaeII ACGT 1 cut(s) 612
MaeIII GTNAC 1 cut(s) 251
MalI GATC 1 cut(s) 506
MboI GATC 1 cut(s) 504
MboII GAAGA 6 cut(s) 12, 48, 67, 235, 457, 473
MluCI AATT 1 cut(s) 362
MmeI TCCRAC 3 cut(s) 84, 319, 519
MnlI CCTC 7 cut(s) 31, 39, 219, 232, 328, 460, 464
Mph1103I ATGCAT 1 cut(s) 181
MroXI GAANNNNTTC 2 cut(s) 74, 406
MseI TTAA 6 cut(s) 81, 156, 182, 231, 528, 648
MslI CAYNNNNRTG 1 cut(s) 489
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 1 cut(s) 346
MspR9I CCNGG 2 cut(s) 198, 248
Mva1269I GAATGC 1 cut(s) 181
MvaI CCWGG 2 cut(s) 198, 248
MwoI GCNNNNNNNGC 2 cut(s) 185, 554
NdeI CATATG 1 cut(s) 441
NdeII GATC 1 cut(s) 504
NlaIII CATG 2 cut(s) 370, 631
NlaIV GGNNCC 1 cut(s) 434
NmuCI GTSAC 1 cut(s) 251
NsiI ATGCAT 1 cut(s) 181
PaqCI CACCTGC 1 cut(s) 587
PctI GAATGC 1 cut(s) 181
PdmI GAANNNNTTC 2 cut(s) 74, 406
PfeI GAWTC 1 cut(s) 542
PkrI GCNGC 1 cut(s) 190
Psp6I CCWGG 2 cut(s) 196, 246
PspFI CCCAGC 1 cut(s) 162
PspGI CCWGG 2 cut(s) 196, 246
PspN4I GGNNCC 1 cut(s) 434
PspPI GGNCC 2 cut(s) 432, 590
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 2 cut(s) 202, 460
RsaNI GTAC 2 cut(s) 201, 459
RseI CAYNNNNRTG 1 cut(s) 489
SaqAI TTAA 6 cut(s) 81, 156, 182, 231, 528, 648
SatI GCNGC 1 cut(s) 189
Sau3AI GATC 1 cut(s) 504
Sau96I GGNCC 2 cut(s) 432, 590
ScrFI CCNGG 2 cut(s) 198, 248
SetI ASST 7 cut(s) 66, 190, 294, 318, 601, 615, 644
SinI GGWCC 1 cut(s) 590
SmiMI CAYNNNNRTG 1 cut(s) 489
Sse9I AATT 1 cut(s) 362
SsiI CCGC 2 cut(s) 138, 652
SspMI CTAG 2 cut(s) 372, 473
StyD4I CCNGG 2 cut(s) 196, 246
StyI CCWWGG 1 cut(s) 582
TaaI ACNGT 3 cut(s) 122, 426, 488
TaiI ACGT 1 cut(s) 615
TaqI TCGA 1 cut(s) 31
TasI AATT 1 cut(s) 362
TfiI GAWTC 1 cut(s) 542
Tru1I TTAA 6 cut(s) 81, 156, 182, 231, 528, 648
Tru9I TTAA 6 cut(s) 81, 156, 182, 231, 528, 648
TscAI CASTG 4 cut(s) 21, 385, 429, 493
TseFI GTSAC 1 cut(s) 251
TseI GCWGC 1 cut(s) 188
Tsp45I GTSAC 1 cut(s) 251
TspDTI ATGAA 6 cut(s) 83, 99, 355, 375, 428, 458
TspRI CASTG 4 cut(s) 21, 385, 429, 493
VpaK11BI GGWCC 1 cut(s) 590
XapI RAATTY 1 cut(s) 362
XmnI GAANNNNTTC 2 cut(s) 74, 406
XspI CTAG 2 cut(s) 372, 473
Zsp2I ATGCAT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.