Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 869 |
| Acc36I | ACCTGC | 2 cut(s) | 726, 869 |
| Acc65I | GGTACC | 1 cut(s) | 33 |
| AccB1I | GGYRCC | 1 cut(s) | 33 |
| AccI | GTMKAC | 1 cut(s) | 1065 |
| AciI | CCGC | 3 cut(s) | 122, 660, 876 |
| AclWI | GGATC | 1 cut(s) | 1112 |
| AcsI | RAATTY | 3 cut(s) | 555, 644, 1057 |
| AcuI | CTGAAG | 2 cut(s) | 1232, 1278 |
| AfaI | GTAC | 3 cut(s) | 19, 35, 1139 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 484, 485 |
| AgsI | TTSAA | 9 cut(s) | 186, 267, 367, 442, 617, 644, 701, 828, 900 |
| AjnI | CCWGG | 2 cut(s) | 51, 478 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 411, 443 |
| AleI | CACNNNNGTG | 1 cut(s) | 771 |
| AluBI | AGCT | 6 cut(s) | 128, 290, 470, 1154, 1166, 1246 |
| AluI | AGCT | 6 cut(s) | 128, 290, 470, 1154, 1166, 1246 |
| Alw21I | GWGCWC | 1 cut(s) | 1156 |
| Alw26I | GTCTC | 3 cut(s) | 89, 145, 657 |
| AlwI | GGATC | 1 cut(s) | 1112 |
| AoxI | GGCC | 4 cut(s) | 119, 1071, 1132, 1208 |
| ApeKI | GCWGC | 7 cut(s) | 248, 470, 574, 743, 929, 1163, 1243 |
| ApoI | RAATTY | 3 cut(s) | 555, 644, 1057 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 688 |
| Asp718I | GGTACC | 1 cut(s) | 33 |
| AspA2I | CCTAGG | 1 cut(s) | 565 |
| AspS9I | GGNCC | 3 cut(s) | 119, 1030, 1071 |
| AsuC2I | CCSGG | 1 cut(s) | 168 |
| AsuHPI | GGTGA | 4 cut(s) | 100, 221, 785, 893 |
| AvaII | GGWCC | 1 cut(s) | 1030 |
| AvrII | CCTAGG | 1 cut(s) | 565 |
| BanI | GGYRCC | 1 cut(s) | 33 |
| BanII | GRGCYC | 2 cut(s) | 201, 1156 |
| BauI | CACGAG | 2 cut(s) | 536, 654 |
| BbsI | GAAGAC | 4 cut(s) | 309, 689, 1024, 1266 |
| Bbv12I | GWGCWC | 1 cut(s) | 1156 |
| BbvI | GCAGC | 7 cut(s) | 235, 457, 561, 730, 916, 1175, 1255 |
| BccI | CCATC | 5 cut(s) | 107, 405, 798, 863, 904 |
| BciT130I | CCWGG | 2 cut(s) | 53, 480 |
| BclI | TGATCA | 1 cut(s) | 622 |
| BcnI | CCSGG | 1 cut(s) | 168 |
| BcoDI | GTCTC | 3 cut(s) | 89, 145, 657 |
| BfaI | CTAG | 3 cut(s) | 566, 755, 1272 |
| BfmI | CTRYAG | 2 cut(s) | 283, 948 |
| BfuAI | ACCTGC | 2 cut(s) | 726, 869 |
| BlnI | CCTAGG | 1 cut(s) | 565 |
| BmcAI | AGTACT | 1 cut(s) | 1139 |
| Bme1390I | CCNGG | 3 cut(s) | 53, 168, 480 |
| Bme18I | GGWCC | 1 cut(s) | 1030 |
| BmgT120I | GGNCC | 3 cut(s) | 119, 1030, 1071 |
| BmiI | GGNNCC | 4 cut(s) | 35, 716, 874, 1254 |
| BmrFI | CCNGG | 3 cut(s) | 53, 168, 480 |
| BmrI | ACTGGG | 3 cut(s) | 72, 126, 1078 |
| BmsI | GCATC | 2 cut(s) | 298, 987 |
| BmuI | ACTGGG | 3 cut(s) | 72, 126, 1078 |
| BpiI | GAAGAC | 4 cut(s) | 309, 689, 1024, 1266 |
| BpmI | CTGGAG | 1 cut(s) | 1170 |
| Bpu10I | CCTNAGC | 1 cut(s) | 350 |
| BpuEI | CTTGAG | 1 cut(s) | 758 |
| BpuMI | CCSGG | 1 cut(s) | 168 |
| BsaBI | GATNNNNATC | 1 cut(s) | 624 |
| BsaI | GGTCTC | 2 cut(s) | 89, 145 |
| BsaJI | CCNNGG | 4 cut(s) | 167, 326, 478, 565 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 484, 485 |
| Bse1I | ACTGG | 5 cut(s) | 67, 121, 392, 1073, 1135 |
| Bse3DI | GCAATG | 2 cut(s) | 250, 548 |
| Bse8I | GATNNNNATC | 1 cut(s) | 624 |
| BseBI | CCWGG | 2 cut(s) | 53, 480 |
| BseDI | CCNNGG | 4 cut(s) | 167, 326, 478, 565 |
| BseGI | GGATG | 4 cut(s) | 163, 397, 580, 874 |
| BseJI | GATNNNNATC | 1 cut(s) | 624 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 484, 485 |
| BseMI | GCAATG | 2 cut(s) | 250, 548 |
| BseMII | CTCAG | 4 cut(s) | 214, 927, 1191, 1212 |
| BseNI | ACTGG | 5 cut(s) | 67, 121, 392, 1073, 1135 |
| BseXI | GCAGC | 7 cut(s) | 235, 457, 561, 730, 916, 1175, 1255 |
| BshFI | GGCC | 4 cut(s) | 121, 1073, 1134, 1210 |
| BshNI | GGYRCC | 1 cut(s) | 33 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1156 |
| BsiSI | CCGG | 1 cut(s) | 167 |
| BslFI | GGGAC | 3 cut(s) | 184, 809, 928 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 484, 485 |
| BsmAI | GTCTC | 3 cut(s) | 89, 145, 657 |
| BsmFI | GGGAC | 3 cut(s) | 184, 809, 928 |
| BsmI | GAATGC | 1 cut(s) | 219 |
| BsnI | GGCC | 4 cut(s) | 121, 1073, 1134, 1210 |
| Bso31I | GGTCTC | 2 cut(s) | 89, 145 |
| Bsp1286I | GDGCHC | 2 cut(s) | 201, 1156 |
| Bsp143I | GATC | 3 cut(s) | 160, 622, 1117 |
| BspACI | CCGC | 3 cut(s) | 122, 660, 876 |
| BspANI | GGCC | 4 cut(s) | 121, 1073, 1134, 1210 |
| BspCNI | CTCAG | 4 cut(s) | 213, 928, 1192, 1213 |
| BspLI | GGNNCC | 4 cut(s) | 35, 716, 874, 1254 |
| BspMI | ACCTGC | 2 cut(s) | 726, 869 |
| BspPI | GGATC | 1 cut(s) | 1112 |
| BspT107I | GGYRCC | 1 cut(s) | 33 |
| BspTNI | GGTCTC | 2 cut(s) | 89, 145 |
| BsrDI | GCAATG | 2 cut(s) | 250, 548 |
| BsrI | ACTGG | 5 cut(s) | 67, 121, 392, 1073, 1135 |
| BssECI | CCNNGG | 4 cut(s) | 167, 326, 478, 565 |
| BssMI | GATC | 3 cut(s) | 160, 622, 1117 |
| BssSI | CACGAG | 2 cut(s) | 536, 654 |
| BssT1I | CCWWGG | 1 cut(s) | 565 |
| Bst2BI | CACGAG | 2 cut(s) | 536, 654 |
| Bst2UI | CCWGG | 2 cut(s) | 53, 480 |
| Bst4CI | ACNGT | 3 cut(s) | 79, 784, 1048 |
| Bst6I | CTCTTC | 1 cut(s) | 500 |
| BstC8I | GCNNGC | 3 cut(s) | 126, 1040, 1090 |
| BstDEI | CTNAG | 5 cut(s) | 200, 350, 936, 1200, 1221 |
| BstF5I | GGATG | 4 cut(s) | 163, 397, 580, 874 |
| BstKTI | GATC | 3 cut(s) | 163, 625, 1120 |
| BstMAI | GTCTC | 3 cut(s) | 89, 145, 657 |
| BstMBI | GATC | 3 cut(s) | 160, 622, 1117 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 845, 1006, 1048, 1160, 1172 |
| BstNI | CCWGG | 2 cut(s) | 53, 480 |
| BstNSI | RCATGY | 1 cut(s) | 493 |
| BstSCI | CCNGG | 3 cut(s) | 51, 166, 478 |
| BstSFI | CTRYAG | 2 cut(s) | 283, 948 |
| BstV1I | GCAGC | 7 cut(s) | 235, 457, 561, 730, 916, 1175, 1255 |
| BstV2I | GAAGAC | 4 cut(s) | 309, 689, 1024, 1266 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 52, 711 |
| BsuRI | GGCC | 4 cut(s) | 121, 1073, 1134, 1210 |
| BtsCI | GGATG | 4 cut(s) | 163, 397, 580, 874 |
| BtsI | GCAGTG | 2 cut(s) | 318, 912 |
| BtsIMutI | CAGTG | 4 cut(s) | 60, 84, 318, 912 |
| BveI | ACCTGC | 2 cut(s) | 726, 869 |
| Cac8I | GCNNGC | 3 cut(s) | 126, 1040, 1090 |
| Cfr13I | GGNCC | 3 cut(s) | 119, 1030, 1071 |
| CseI | GACGC | 1 cut(s) | 566 |
| Csp6I | GTAC | 3 cut(s) | 18, 34, 1138 |
| CviAII | CATG | 9 cut(s) | 83, 259, 305, 490, 711, 893, 920, 1216, 1264 |
| CviQI | GTAC | 3 cut(s) | 18, 34, 1138 |
| DdeI | CTNAG | 5 cut(s) | 200, 350, 936, 1200, 1221 |
| DpnI | GATC | 3 cut(s) | 162, 624, 1119 |
| DpnII | GATC | 3 cut(s) | 160, 622, 1117 |
| DraI | TTTAAA | 1 cut(s) | 1128 |
| Eam1104I | CTCTTC | 1 cut(s) | 500 |
| EarI | CTCTTC | 1 cut(s) | 500 |
| Ecl136II | GAGCTC | 1 cut(s) | 1154 |
| Eco130I | CCWWGG | 1 cut(s) | 565 |
| Eco24I | GRGCYC | 2 cut(s) | 201, 1156 |
| Eco31I | GGTCTC | 2 cut(s) | 89, 145 |
| Eco47I | GGWCC | 1 cut(s) | 1030 |
| Eco53kI | GAGCTC | 1 cut(s) | 1154 |
| Eco57I | CTGAAG | 2 cut(s) | 1232, 1278 |
| EcoICRI | GAGCTC | 1 cut(s) | 1154 |
| EcoRII | CCWGG | 2 cut(s) | 51, 478 |
| EcoT14I | CCWWGG | 1 cut(s) | 565 |
| EcoT38I | GRGCYC | 2 cut(s) | 201, 1156 |
| ErhI | CCWWGG | 1 cut(s) | 565 |
| FaeI | CATG | 9 cut(s) | 86, 262, 308, 493, 714, 896, 923, 1219, 1267 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 676, 708 |
| FaqI | GGGAC | 3 cut(s) | 184, 809, 928 |
| FatI | CATG | 9 cut(s) | 82, 258, 304, 489, 710, 892, 919, 1215, 1263 |
| FauNDI | CATATG | 1 cut(s) | 193 |
| FbaI | TGATCA | 1 cut(s) | 622 |
| FblI | GTMKAC | 1 cut(s) | 1065 |
| FokI | GGATG | 4 cut(s) | 150, 384, 567, 881 |
| FriOI | GRGCYC | 2 cut(s) | 201, 1156 |
| FspBI | CTAG | 3 cut(s) | 566, 755, 1272 |
| GsuI | CTGGAG | 1 cut(s) | 1170 |
| HaeIII | GGCC | 4 cut(s) | 121, 1073, 1134, 1210 |
| HapII | CCGG | 1 cut(s) | 167 |
| HgaI | GACGC | 1 cut(s) | 566 |
| Hin1II | CATG | 9 cut(s) | 86, 262, 308, 493, 714, 896, 923, 1219, 1267 |
| HincII | GTYRAC | 1 cut(s) | 1066 |
| HindII | GTYRAC | 1 cut(s) | 1066 |
| HindIII | AAGCTT | 1 cut(s) | 126 |
| HinfI | GANTC | 9 cut(s) | 13, 175, 263, 279, 295, 357, 458, 689, 824 |
| HpaII | CCGG | 1 cut(s) | 167 |
| HphI | GGTGA | 4 cut(s) | 100, 221, 785, 893 |
| Hpy166II | GTNNAC | 1 cut(s) | 1066 |
| Hpy188I | TCNGA | 3 cut(s) | 133, 330, 1192 |
| Hpy188III | TCNNGA | 8 cut(s) | 364, 401, 536, 794, 935, 1014, 1028, 1250 |
| Hpy8I | GTNNAC | 1 cut(s) | 1066 |
| HpyAV | CCTTC | 8 cut(s) | 22, 329, 413, 515, 564, 678, 859, 1261 |
| HpyCH4III | ACNGT | 3 cut(s) | 79, 784, 1048 |
| HpyCH4IV | ACGT | 1 cut(s) | 1021 |
| HpyCH4V | TGCA | 5 cut(s) | 82, 251, 311, 1051, 1175 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 845, 1006, 1048, 1160, 1172 |
| HpyF3I | CTNAG | 5 cut(s) | 200, 350, 936, 1200, 1221 |
| HpySE526I | ACGT | 1 cut(s) | 1021 |
| Hsp92II | CATG | 9 cut(s) | 86, 262, 308, 493, 714, 896, 923, 1219, 1267 |
| KpnI | GGTACC | 1 cut(s) | 37 |
| Ksp22I | TGATCA | 1 cut(s) | 622 |
| Kzo9I | GATC | 3 cut(s) | 160, 622, 1117 |
| LmnI | GCTCC | 4 cut(s) | 872, 937, 1151, 1240 |
| Lsp1109I | GCAGC | 7 cut(s) | 235, 457, 561, 730, 916, 1175, 1255 |
| LweI | GCATC | 2 cut(s) | 298, 987 |
| MaeI | CTAG | 3 cut(s) | 566, 755, 1272 |
| MaeII | ACGT | 1 cut(s) | 1021 |
| MaeIII | GTNAC | 1 cut(s) | 533 |
| MalI | GATC | 3 cut(s) | 162, 624, 1119 |
| MboI | GATC | 3 cut(s) | 160, 622, 1117 |
| MhlI | GDGCHC | 2 cut(s) | 201, 1156 |
| MluCI | AATT | 7 cut(s) | 69, 109, 268, 555, 596, 644, 1057 |
| MlyI | GAGTC | 3 cut(s) | 184, 304, 452 |
| MmeI | TCCRAC | 1 cut(s) | 801 |
| MnlI | CCTC | 8 cut(s) | 79, 336, 501, 733, 769, 1094, 1131, 1216 |
| MroXI | GAANNNNTTC | 1 cut(s) | 688 |
| MseI | TTAA | 5 cut(s) | 375, 513, 959, 1127, 1158 |
| MslI | CAYNNNNRTG | 3 cut(s) | 709, 771, 924 |
| MspA1I | CMGCKG | 1 cut(s) | 470 |
| MspI | CCGG | 1 cut(s) | 167 |
| MspR9I | CCNGG | 3 cut(s) | 53, 168, 480 |
| Mva1269I | GAATGC | 1 cut(s) | 219 |
| MvaI | CCWGG | 2 cut(s) | 53, 480 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 845, 1006, 1048, 1160, 1172 |
| NciI | CCSGG | 1 cut(s) | 168 |
| NdeI | CATATG | 1 cut(s) | 193 |
| NdeII | GATC | 3 cut(s) | 160, 622, 1117 |
| NlaIII | CATG | 9 cut(s) | 86, 262, 308, 493, 714, 896, 923, 1219, 1267 |
| NlaIV | GGNNCC | 4 cut(s) | 35, 716, 874, 1254 |
| NmuCI | GTSAC | 1 cut(s) | 533 |
| NspI | RCATGY | 1 cut(s) | 493 |
| OliI | CACNNNNGTG | 1 cut(s) | 771 |
| PaqCI | CACCTGC | 1 cut(s) | 869 |
| PctI | GAATGC | 1 cut(s) | 219 |
| PdmI | GAANNNNTTC | 1 cut(s) | 688 |
| PfeI | GAWTC | 6 cut(s) | 13, 263, 279, 357, 689, 824 |
| PflFI | GACNNNGTC | 1 cut(s) | 1023 |
| PleI | GAGTC | 3 cut(s) | 183, 303, 452 |
| PpsI | GAGTC | 3 cut(s) | 183, 303, 452 |
| Psp124BI | GAGCTC | 1 cut(s) | 1156 |
| Psp6I | CCWGG | 2 cut(s) | 51, 478 |
| PspGI | CCWGG | 2 cut(s) | 51, 478 |
| PspN4I | GGNNCC | 4 cut(s) | 35, 716, 874, 1254 |
| PspPI | GGNCC | 3 cut(s) | 119, 1030, 1071 |
| PsyI | GACNNNGTC | 1 cut(s) | 1023 |
| PvuII | CAGCTG | 1 cut(s) | 470 |
| RsaI | GTAC | 3 cut(s) | 19, 35, 1139 |
| RsaNI | GTAC | 3 cut(s) | 18, 34, 1138 |
| RseI | CAYNNNNRTG | 3 cut(s) | 709, 771, 924 |
| SacI | GAGCTC | 1 cut(s) | 1156 |
| SalI | GTCGAC | 1 cut(s) | 1064 |
| SaqAI | TTAA | 5 cut(s) | 375, 513, 959, 1127, 1158 |
| Sau3AI | GATC | 3 cut(s) | 160, 622, 1117 |
| Sau96I | GGNCC | 3 cut(s) | 119, 1030, 1071 |
| ScaI | AGTACT | 1 cut(s) | 1139 |
| SchI | GAGTC | 3 cut(s) | 184, 304, 452 |
| ScrFI | CCNGG | 3 cut(s) | 53, 168, 480 |
| SduI | GDGCHC | 2 cut(s) | 201, 1156 |
| SfaNI | GCATC | 2 cut(s) | 298, 987 |
| SfcI | CTRYAG | 2 cut(s) | 283, 948 |
| SinI | GGWCC | 1 cut(s) | 1030 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 709, 771, 924 |
| SmlI | CTYRAG | 1 cut(s) | 737 |
| SmoI | CTYRAG | 1 cut(s) | 737 |
| Sse9I | AATT | 7 cut(s) | 69, 109, 268, 555, 596, 644, 1057 |
| SsiI | CCGC | 3 cut(s) | 122, 660, 876 |
| SspMI | CTAG | 3 cut(s) | 566, 755, 1272 |
| SstI | GAGCTC | 1 cut(s) | 1156 |
| StyD4I | CCNGG | 3 cut(s) | 51, 166, 478 |
| StyI | CCWWGG | 1 cut(s) | 565 |
| TaaI | ACNGT | 3 cut(s) | 79, 784, 1048 |
| TaiI | ACGT | 1 cut(s) | 1024 |
| TaqI | TCGA | 2 cut(s) | 1002, 1065 |
| TasI | AATT | 7 cut(s) | 69, 109, 268, 555, 596, 644, 1057 |
| TatI | WGTACW | 1 cut(s) | 1137 |
| TauI | GCSGC | 3 cut(s) | 124, 663, 878 |
| TfiI | GAWTC | 6 cut(s) | 13, 263, 279, 357, 689, 824 |
| Tru1I | TTAA | 5 cut(s) | 375, 513, 959, 1127, 1158 |
| Tru9I | TTAA | 5 cut(s) | 375, 513, 959, 1127, 1158 |
| TscAI | CASTG | 4 cut(s) | 67, 84, 318, 912 |
| TseFI | GTSAC | 1 cut(s) | 533 |
| TseI | GCWGC | 7 cut(s) | 248, 470, 574, 743, 929, 1163, 1243 |
| Tsp45I | GTSAC | 1 cut(s) | 533 |
| TspDTI | ATGAA | 5 cut(s) | 180, 384, 699, 740, 1280 |
| TspRI | CASTG | 4 cut(s) | 67, 84, 318, 912 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1023 |
| VpaK11BI | GGWCC | 1 cut(s) | 1030 |
| XapI | RAATTY | 3 cut(s) | 555, 644, 1057 |
| XceI | RCATGY | 1 cut(s) | 493 |
| XmaJI | CCTAGG | 1 cut(s) | 565 |
| XmiI | GTMKAC | 1 cut(s) | 1065 |
| XmnI | GAANNNNTTC | 1 cut(s) | 688 |
| XspI | CTAG | 3 cut(s) | 566, 755, 1272 |
| ZrmI | AGTACT | 1 cut(s) | 1139 |