RchiOBHm_Chr1g0367251
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000305
Physical Location & Seq
Forward (+)
57592296 .. 57595309
3014 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59172

Sequence Viewer

Length: 1062 bp
ATGGGTGGAGGAATTCGCACAAGCGTGAAGGGGTACCTCCTTTCGCAGCGGATCACTACGGCTTCAGACACAGTGCATGCAAACGAGAGCACCAATAGAAATTTCACTGCACCCCAAGCTGCTGATTCAGAACAAGAGACGAGCACGGACTTGGATTCATCTAACTCATATTGGCTCAGTAGGTTTAGTGGTAGCATTCCTTGCTTGCCGATATTGGGCTCTGCTTCAAAGAGCAGGGTTTCAAATAGAACTAGAATCAGAGCTGAGAGTCTGCCTCACAGAGTGCCTTCAACTCCTAGGACAGAGGTTGAGATCTTGCTAAAGAGCTTCTTTTTTAATGAACTGAAAACCGCCACCAGCAACTTTCGGCCTGATAATGTGGTGGGTGAGGGTGGTTTTGGTACCGTCTTTAAGGGGTGGCTTGATCAGAATTCATTAGCAGCTGCCAAGCCTGGTACAGGAATTGCTGTTGCTGTAAAGAGGCTTAACCAAGAACGTCATCAGGGTCGGGAGGAATGGTTTACAGAAATTTACTACCTTGGACAGCTGCGTCACCCAAATCTTGTGAAGTTGATAGGCTATTGTTCAGAGGATAATGAACTGCTTCTGGTGTATGAATTTATGCCCCGTGGCAGTTTGGAGAATCATATATTTAGAAGGAGTTCTCACTTTCAACCACTTTCGTGGACTGTTCGTATGAAGATTGCTCTTGGTGCTGCAAAGGCTCTAGCATTTCTTCACAATGAAGCAAAAGTTATACATCGTGACTTTAAAACATCTAATATCCTGTTGGATTCAACCTACAATGTTAAAATCTCTGATTTTGGTTTGGCTGTGGATGCACGAGCATTTGACAGACACCGAGGTGCTAAAACAAGGGTCAAGGGCACATATGGATATGCAGCTCCTGAGTATATAGAAACAAGGGTCATGGGCACATATGGATATGCAGCTCCTGAGTATGTAGCTACAGGTATCTTAGTTCATAAAAATATTTTTGTTCAAATACAGAAGCAATATACCCTGGAGAAAGGTACAAAAGTATATCATTCTTCAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0035821 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0046777 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0060089 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0075136 GO:0080134 GO:0098542 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

353

Amino Acids

39.52

Weight (kDa)

9.52

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 125 - 311 3.8e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 126 - 311 2.7e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000305)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39660 AT3G55450 AT3G55450
fragaria_vesca FvH4_7g22540 FvH4_7g22540 FvH4_7g22540 FvH4_7g22540 FvH4_7g22550 FvH4_7g22550 FvH4_7g22590 FvH4_7g22592 FvH4_7g22592 FvH4_7g22593 FvH4_7g22593 FvH4_7g22593
malus_domestica MD01G1134700.v1.1 MD07G1199400.v1.1
prunus_persica Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1
pyrus_communis pycom01g15540 pycom07g18630
rosa_chinensis RchiOBHm_Chr1g0367221 RchiOBHm_Chr1g0367231 RchiOBHm_Chr1g0367251 RchiOBHm_Chr1g0367261 RchiOBHm_Chr1g0367311 RchiOBHm_Chr1g0367321 RchiOBHm_Chr1g0367351 RchiOBHm_Chr1g0367401 RchiOBHm_Chr1g0367411 RchiOBHm_Chr1g0367581 RchiOBHm_Chr5g0029531
rosa_laevigata RLG00000001400 RLG00000024338 RLG00000027271 RLG00000027274 RLG00000027277 RLG00000027278 RLG00000027280 RLG00000027281 RLG00000027282 RLG00000027285 RLG00000027286 RLG00000027288 RLG00000033176
rosa_multiflora Rmu_sc0002690.1_g000002 Rmu_sc0004181.1_g000007 Rmu_sc0008470.1_g000013 Rmu_sc0008470.1_g000015 Rmu_sc0009775.1_g000001 Rmu_sc0009775.1_g000003 Rmu_sc0009775.1_g000004 Rmu_sc0009775.1_g000005 Rmu_sc0011000.1_g000008 Rmu_sc0014291.1_g000001 Rmu_sc0014291.1_g000005 Rmu_sc0014291.1_g000008
rosa_roxburghii Rroxscaffold_1G00050440 Rroxscaffold_4G00289340 Rroxscaffold_4G00289350 Rroxscaffold_4G00289380 Rroxscaffold_4G00289400 Rroxscaffold_4G00289410 Rroxscaffold_4G00289450 Rroxscaffold_4G00289490 Rroxscaffold_4G00289500 Rroxscaffold_4G00289510 Rroxscaffold_6G00420130 Rroxscaffold_6G00421370
rosa_rugosa Rorug01G0335600.1 Rorug01G0335700 Rorug01G0335800 Rorug01G0335800 Rorug01G0335800 Rorug01G0335800 Rorug01G0335900 Rorug01G0335900 Rorug01G0336000 Rorug01G0336000 Rorug05G0114700 Rorug05G0114800
rosa_samantha Rh1AG342300 Rh1AG342500 Rh1AG342700 Rh1BG302700 Rh1BG303000 Rh1BG303100 Rh1BG303400 Rh1BG303500 Rh1BG303800 Rh1BG304100 Rh1CG319200 Rh1CG319400 Rh1CG319800 Rh1CG319900 Rh1DG335300 Rh1DG335500 Rh1DG335900 Rh1DG336200 Rh2AG106400 Rh3DG307700 Rh4CG281300 Rh5AG206700 Rh5CG227400 Rh5DG209100 Rh7CG338300
rosa_wichuraiana Rw1G030420 Rw1G030430 Rw1G030460 Rw1G030470 Rw1G030480 Rw1G030510 Rw1G030520 Rw5G018800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 549
Acc65I GGTACC 2 cut(s) 33, 401
AccB1I GGYRCC 2 cut(s) 33, 401
AciI CCGC 2 cut(s) 49, 351
AclWI GGATC 1 cut(s) 59
AcsI RAATTY 5 cut(s) 12, 100, 430, 528, 617
AcuI CTGAAG 1 cut(s) 48
AdeI CACNNNGTG 1 cut(s) 283
AfaI GTAC 4 cut(s) 35, 403, 457, 1036
AfiI CCNNNNNNNGG 2 cut(s) 215, 458
AgsI TTSAA 7 cut(s) 228, 243, 291, 674, 798, 1004, 1056
AjnI CCWGG 2 cut(s) 451, 1023
AjuI GAANNNNNNNTTGG 2 cut(s) 86, 118
AleI CACNNNNGTG 3 cut(s) 23, 682, 864
AluBI AGCT 8 cut(s) 119, 263, 327, 443, 547, 905, 953, 968
AluI AGCT 8 cut(s) 119, 263, 327, 443, 547, 905, 953, 968
Alw21I GWGCWC 2 cut(s) 92, 146
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 1 cut(s) 59
AlwNI CAGNNNCTG 2 cut(s) 908, 956
AoxI GGCC 1 cut(s) 368
ApeKI GCWGC 8 cut(s) 46, 119, 440, 443, 547, 716, 902, 950
ApoI RAATTY 5 cut(s) 12, 100, 430, 528, 617
Asp700I GAANNNNTTC 2 cut(s) 603, 661
Asp718I GGTACC 2 cut(s) 33, 401
AspA2I CCTAGG 1 cut(s) 296
AsuHPI GGTGA 2 cut(s) 398, 545
AvrII CCTAGG 1 cut(s) 296
BaeGI GKGCMC 2 cut(s) 890, 938
BaeI ACNNNNGTAYC 2 cut(s) 1026, 1059
BanI GGYRCC 2 cut(s) 33, 401
BanII GRGCYC 1 cut(s) 221
BauI CACGAG 1 cut(s) 843
Bbv12I GWGCWC 2 cut(s) 92, 146
BbvI GCAGC 8 cut(s) 58, 106, 430, 452, 534, 703, 914, 962
BceAI ACGGC 1 cut(s) 75
BciT130I CCWGG 2 cut(s) 453, 1025
BclI TGATCA 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 3 cut(s) 252, 297, 728
BfmI CTRYAG 1 cut(s) 969
BglII AGATCT 1 cut(s) 312
BisI GCNGC 8 cut(s) 47, 120, 441, 444, 548, 717, 903, 951
BlnI CCTAGG 1 cut(s) 296
BlsI GCNGC 8 cut(s) 48, 121, 442, 445, 549, 718, 904, 952
Bme1390I CCNGG 2 cut(s) 453, 1025
BmiI GGNNCC 2 cut(s) 35, 403
BmrFI CCNGG 2 cut(s) 453, 1025
BmsI GCATC 1 cut(s) 829
BplI GAGNNNNNCTC 2 cut(s) 259, 291
BpmI CTGGAG 1 cut(s) 1046
BsaJI CCNNGG 5 cut(s) 296, 538, 628, 862, 1023
BsaXI ACNNNNNCTCC 2 cut(s) 503, 533
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 458
BseBI CCWGG 2 cut(s) 453, 1025
BseDI CCNNGG 5 cut(s) 296, 538, 628, 862, 1023
BseGI GGATG 1 cut(s) 844
BseLI CCNNNNNNNGG 2 cut(s) 215, 458
BseMII CTCAG 4 cut(s) 190, 255, 900, 948
BseSI GKGCMC 2 cut(s) 890, 938
BseXI GCAGC 8 cut(s) 58, 106, 430, 452, 534, 703, 914, 962
BsgI GTGCAG 1 cut(s) 93
BshFI GGCC 1 cut(s) 370
BshNI GGYRCC 2 cut(s) 33, 401
BsiHKAI GWGCWC 2 cut(s) 92, 146
BslI CCNNNNNNNGG 2 cut(s) 215, 458
BsmAI GTCTC 1 cut(s) 131
BsmBI CGTCTC 1 cut(s) 131
BsmI GAATGC 1 cut(s) 195
BsnI GGCC 1 cut(s) 370
Bsp1286I GDGCHC 5 cut(s) 92, 146, 221, 890, 938
Bsp143I GATC 3 cut(s) 51, 312, 424
BspACI CCGC 2 cut(s) 49, 351
BspANI GGCC 1 cut(s) 370
BspCNI CTCAG 4 cut(s) 189, 256, 901, 949
BspLI GGNNCC 2 cut(s) 35, 403
BspPI GGATC 1 cut(s) 59
BspT107I GGYRCC 2 cut(s) 33, 401
BssECI CCNNGG 5 cut(s) 296, 538, 628, 862, 1023
BssMI GATC 3 cut(s) 51, 312, 424
BssSI CACGAG 1 cut(s) 843
BssT1I CCWWGG 2 cut(s) 296, 538
Bst2BI CACGAG 1 cut(s) 843
Bst2UI CCWGG 2 cut(s) 453, 1025
Bst4CI ACNGT 3 cut(s) 73, 406, 691
BstAPI GCANNNNNTGC 1 cut(s) 201
BstC8I GCNNGC 2 cut(s) 78, 206
BstDEI CTNAG 5 cut(s) 176, 264, 909, 957, 979
BstDSI CCRYGG 1 cut(s) 628
BstENI CCTNNNNNAGG 1 cut(s) 456
BstF5I GGATG 1 cut(s) 844
BstKTI GATC 3 cut(s) 54, 315, 427
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 3 cut(s) 51, 312, 424
BstMWI GCNNNNNNNGC 5 cut(s) 116, 201, 713, 722, 839
BstNI CCWGG 2 cut(s) 453, 1025
BstNSI RCATGY 1 cut(s) 80
BstSCI CCNGG 2 cut(s) 451, 1023
BstSFI CTRYAG 1 cut(s) 969
BstSLI GKGCMC 2 cut(s) 890, 938
BstV1I GCAGC 8 cut(s) 58, 106, 430, 452, 534, 703, 914, 962
BstX2I RGATCY 1 cut(s) 312
BstXI CCANNNNNNTGG 1 cut(s) 684
BstYI RGATCY 1 cut(s) 312
BsuRI GGCC 1 cut(s) 370
BtgI CCRYGG 1 cut(s) 628
BtsCI GGATG 1 cut(s) 844
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 2 cut(s) 78, 105
Cac8I GCNNGC 2 cut(s) 78, 206
CaiI CAGNNNCTG 2 cut(s) 908, 956
CseI GACGC 1 cut(s) 539
Csp6I GTAC 4 cut(s) 34, 402, 456, 1035
CviAII CATG 2 cut(s) 77, 931
CviQI GTAC 4 cut(s) 34, 402, 456, 1035
DdeI CTNAG 5 cut(s) 176, 264, 909, 957, 979
DpnI GATC 3 cut(s) 53, 314, 426
DpnII GATC 3 cut(s) 51, 312, 424
DraI TTTAAA 1 cut(s) 772
DraIII CACNNNGTG 1 cut(s) 283
DrdI GACNNNNNNGTC 1 cut(s) 549
DseDI GACNNNNNNGTC 1 cut(s) 549
Eco130I CCWWGG 2 cut(s) 296, 538
Eco24I GRGCYC 1 cut(s) 221
Eco57I CTGAAG 1 cut(s) 48
EcoNI CCTNNNNNAGG 1 cut(s) 456
EcoRI GAATTC 2 cut(s) 12, 430
EcoRII CCWGG 2 cut(s) 451, 1023
EcoT14I CCWWGG 2 cut(s) 296, 538
EcoT38I GRGCYC 1 cut(s) 221
ErhI CCWWGG 2 cut(s) 296, 538
Esp3I CGTCTC 1 cut(s) 131
FaeI CATG 2 cut(s) 80, 934
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FatI CATG 2 cut(s) 76, 930
FauNDI CATATG 2 cut(s) 892, 940
FbaI TGATCA 1 cut(s) 424
Fnu4HI GCNGC 8 cut(s) 47, 120, 441, 444, 548, 717, 903, 951
FokI GGATG 1 cut(s) 851
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 8 cut(s) 47, 120, 441, 444, 548, 717, 903, 951
FspBI CTAG 3 cut(s) 252, 297, 728
GluI GCNGC 8 cut(s) 47, 120, 441, 444, 548, 717, 903, 951
GsuI CTGGAG 1 cut(s) 1046
HaeIII GGCC 1 cut(s) 370
HgaI GACGC 1 cut(s) 539
Hin1II CATG 2 cut(s) 80, 934
HinfI GANTC 6 cut(s) 125, 155, 255, 268, 643, 794
HphI GGTGA 2 cut(s) 398, 545
Hpy166II GTNNAC 2 cut(s) 522, 687
Hpy188I TCNGA 6 cut(s) 67, 130, 260, 429, 589, 820
Hpy188III TCNNGA 4 cut(s) 509, 764, 908, 956
Hpy8I GTNNAC 2 cut(s) 522, 687
HpyAV CCTTC 3 cut(s) 22, 297, 651
HpyCH4III ACNGT 3 cut(s) 73, 406, 691
HpyCH4IV ACGT 1 cut(s) 496
HpyCH4V TGCA 7 cut(s) 76, 80, 110, 719, 842, 902, 950
HpyF10VI GCNNNNNNNGC 5 cut(s) 116, 201, 713, 722, 839
HpyF3I CTNAG 5 cut(s) 176, 264, 909, 957, 979
HpySE526I ACGT 1 cut(s) 496
Hsp92II CATG 2 cut(s) 80, 934
KpnI GGTACC 2 cut(s) 37, 405
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 3 cut(s) 51, 312, 424
LmnI GCTCC 2 cut(s) 910, 958
Lsp1109I GCAGC 8 cut(s) 58, 106, 430, 452, 534, 703, 914, 962
LweI GCATC 1 cut(s) 829
MaeI CTAG 3 cut(s) 252, 297, 728
MaeII ACGT 1 cut(s) 496
MaeIII GTNAC 2 cut(s) 551, 764
MalI GATC 3 cut(s) 53, 314, 426
MboI GATC 3 cut(s) 51, 312, 424
MboII GAAGA 3 cut(s) 712, 728, 1044
MflI RGATCY 1 cut(s) 312
MhlI GDGCHC 5 cut(s) 92, 146, 221, 890, 938
MluCI AATT 7 cut(s) 12, 100, 430, 462, 528, 617, 1056
MlyI GAGTC 1 cut(s) 277
MmeI TCCRAC 1 cut(s) 771
MnlI CCTC 8 cut(s) 47, 285, 298, 382, 474, 505, 583, 857
MroXI GAANNNNTTC 2 cut(s) 603, 661
MseI TTAA 5 cut(s) 336, 411, 486, 771, 810
MslI CAYNNNNRTG 3 cut(s) 23, 682, 864
MspA1I CMGCKG 3 cut(s) 49, 443, 547
MspR9I CCNGG 2 cut(s) 453, 1025
Mva1269I GAATGC 1 cut(s) 195
MvaI CCWGG 2 cut(s) 453, 1025
MwoI GCNNNNNNNGC 5 cut(s) 116, 201, 713, 722, 839
NdeI CATATG 2 cut(s) 892, 940
NdeII GATC 3 cut(s) 51, 312, 424
NlaIII CATG 2 cut(s) 80, 934
NlaIV GGNNCC 2 cut(s) 35, 403
NmuCI GTSAC 2 cut(s) 551, 764
NspI RCATGY 1 cut(s) 80
OliI CACNNNNGTG 3 cut(s) 23, 682, 864
PaeI GCATGC 1 cut(s) 80
PctI GAATGC 1 cut(s) 195
PdmI GAANNNNTTC 2 cut(s) 603, 661
PfeI GAWTC 5 cut(s) 125, 155, 255, 643, 794
PkrI GCNGC 8 cut(s) 48, 121, 442, 445, 549, 718, 904, 952
PleI GAGTC 1 cut(s) 276
PpsI GAGTC 1 cut(s) 276
Psp6I CCWGG 2 cut(s) 451, 1023
PspGI CCWGG 2 cut(s) 451, 1023
PspN4I GGNNCC 2 cut(s) 35, 403
PstNI CAGNNNCTG 2 cut(s) 908, 956
PsuI RGATCY 1 cut(s) 312
PvuII CAGCTG 2 cut(s) 443, 547
RsaI GTAC 4 cut(s) 35, 403, 457, 1036
RsaNI GTAC 4 cut(s) 34, 402, 456, 1035
RseI CAYNNNNRTG 3 cut(s) 23, 682, 864
SaqAI TTAA 5 cut(s) 336, 411, 486, 771, 810
SatI GCNGC 8 cut(s) 47, 120, 441, 444, 548, 717, 903, 951
Sau3AI GATC 3 cut(s) 51, 312, 424
SchI GAGTC 1 cut(s) 277
ScrFI CCNGG 2 cut(s) 453, 1025
SduI GDGCHC 5 cut(s) 92, 146, 221, 890, 938
SfaNI GCATC 1 cut(s) 829
SfcI CTRYAG 1 cut(s) 969
SmiMI CAYNNNNRTG 3 cut(s) 23, 682, 864
SphI GCATGC 1 cut(s) 80
Sse9I AATT 7 cut(s) 12, 100, 430, 462, 528, 617, 1056
SsiI CCGC 2 cut(s) 49, 351
SspI AATATT 1 cut(s) 994
SspMI CTAG 3 cut(s) 252, 297, 728
StyD4I CCNGG 2 cut(s) 451, 1023
StyI CCWWGG 2 cut(s) 296, 538
TaaI ACNGT 3 cut(s) 73, 406, 691
TaiI ACGT 1 cut(s) 499
TasI AATT 7 cut(s) 12, 100, 430, 462, 528, 617, 1056
TfiI GAWTC 5 cut(s) 125, 155, 255, 643, 794
Tru1I TTAA 5 cut(s) 336, 411, 486, 771, 810
Tru9I TTAA 5 cut(s) 336, 411, 486, 771, 810
TscAI CASTG 2 cut(s) 78, 112
TseFI GTSAC 2 cut(s) 551, 764
TseI GCWGC 8 cut(s) 46, 119, 440, 443, 547, 716, 902, 950
Tsp45I GTSAC 2 cut(s) 551, 764
TspDTI ATGAA 8 cut(s) 147, 354, 423, 612, 630, 713, 759, 974
TspGWI ACGGA 1 cut(s) 161
TspRI CASTG 2 cut(s) 78, 112
XagI CCTNNNNNAGG 1 cut(s) 456
XapI RAATTY 5 cut(s) 12, 100, 430, 528, 617
XceI RCATGY 1 cut(s) 80
XmaJI CCTAGG 1 cut(s) 296
XmnI GAANNNNTTC 2 cut(s) 603, 661
XspI CTAG 3 cut(s) 252, 297, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.