RLG00000027277

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000305
Physical Location & Seq
Reverse (-)
10224349 .. 10233017
8669 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027277

Sequence Viewer

Length: 1263 bp
ATGTGCTTGAGCGATCTTGGGCCTAGGCCCATCACATCGAACATTCGTCTTCCGCTTCTTCTCCGTTCTTCATTTGGAGAAGAAGCCAAATTAACGACGCTAATTCATGGCAGCCAACACCGTTATGACTCCAACCACCGGCTTCTACCGCCGTCCGAACCATTACTCCGGCAGTTGGCCGCCGACTTTAGTCAGGGGGAGATTTTTCCAAGAAGATTCTTCTTTAAAGAACTGAAAACAGCAACCTGGAACTTTCATCCTGATAATATGGTGGGTGAAGGTTGTTATGGTCGTGTTTTTAAGGGTTGGATTGATGATAACACATCAACATCTGCCAAACCCGGTAGTGGCATGTTAATTGCTGTGAAGAAAATTAACCGAGAAGGTTCACAGGGTCACAAGGAATGGTTGACAGAAATTTACTACCTAGGAAGGCTGCATCATCCAAATGTTGTGAAATTGTTGGGTTATTGCTTCGAGGATGACCACCGGCTTCTGGTTTATGAATTTATGGCTAAAGGAAGCTTGGAGTTTCATCTATTTAGATTTGAAATTGGGTTTTCTTACCTTCAACCACTTTCGTGGATCCTGCGTATGAAGATTGCCCTTGGTGCTGCCAAGGGTCTAGCATTTATTCATAACAGTGAGGGAAAAGTGATCCATCGGGACTTCAAAAGTTCTAATATCCTACTGGATTCAAACTACAATGCCAAACTCTCTGATTTTGGTTTTGCTAAGGACGGACCAGCAGGTGATAGTAGCCATGTTTCGACAAGGGTGATGGGGACATATGGATATGCAGCTCCAGAGTATGTTGCTACAGGTCATTTAACCAGCAAAAATGATGTGTATGGTTTCGGAGTTGTTATGCTCGAATTGCTGACAGGAAAACCAGTTCTTGACACTAGCCGGCCAACCGGGGAACAAGATCTAGTTGAATGGGCCAAACCATACCTTGCCAGCAAACGCAGAGTTCTTAAAATTTTTGATGCTCGTATTAAAGGCCAGTACTCTGTGGCTGCAGCTCGTAAAGCAGCTAACCTTGCAAATCAATGCTTATCAGCAGAACCCGAGTTGAGGCCAAACATGAATGAGGTGGTAAAAGAATTAGAGCAGCTTCAGGAATCAGGTGACATTGAGGGCGCAGGAGTCTCTCATAACGAGCCTTGCCAATCTCCTTATTCCAATTCAAGCAGTGTCAGATCAAGCTGGAGAATTTTGATCAGCTTCAGGTCATCTGCTTCTCGCAGCTACATAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

421

Amino Acids

47.12

Weight (kDa)

8.99

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 87 - 368 1.9e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 88 - 370 1.1e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000305)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39660 AT3G55450 AT3G55450
fragaria_vesca FvH4_7g22540 FvH4_7g22540 FvH4_7g22540 FvH4_7g22540 FvH4_7g22550 FvH4_7g22550 FvH4_7g22590 FvH4_7g22592 FvH4_7g22592 FvH4_7g22593 FvH4_7g22593 FvH4_7g22593
malus_domestica MD01G1134700.v1.1 MD07G1199400.v1.1
prunus_persica Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1
pyrus_communis pycom01g15540 pycom07g18630
rosa_chinensis RchiOBHm_Chr1g0367221 RchiOBHm_Chr1g0367231 RchiOBHm_Chr1g0367251 RchiOBHm_Chr1g0367261 RchiOBHm_Chr1g0367311 RchiOBHm_Chr1g0367321 RchiOBHm_Chr1g0367351 RchiOBHm_Chr1g0367401 RchiOBHm_Chr1g0367411 RchiOBHm_Chr1g0367581 RchiOBHm_Chr5g0029531
rosa_laevigata RLG00000001400 RLG00000024338 RLG00000027271 RLG00000027274 RLG00000027277 RLG00000027278 RLG00000027280 RLG00000027281 RLG00000027282 RLG00000027285 RLG00000027286 RLG00000027288 RLG00000033176
rosa_multiflora Rmu_sc0002690.1_g000002 Rmu_sc0004181.1_g000007 Rmu_sc0008470.1_g000013 Rmu_sc0008470.1_g000015 Rmu_sc0009775.1_g000001 Rmu_sc0009775.1_g000003 Rmu_sc0009775.1_g000004 Rmu_sc0009775.1_g000005 Rmu_sc0011000.1_g000008 Rmu_sc0014291.1_g000001 Rmu_sc0014291.1_g000005 Rmu_sc0014291.1_g000008
rosa_roxburghii Rroxscaffold_1G00050440 Rroxscaffold_4G00289340 Rroxscaffold_4G00289350 Rroxscaffold_4G00289380 Rroxscaffold_4G00289400 Rroxscaffold_4G00289410 Rroxscaffold_4G00289450 Rroxscaffold_4G00289490 Rroxscaffold_4G00289500 Rroxscaffold_4G00289510 Rroxscaffold_6G00420130 Rroxscaffold_6G00421370
rosa_rugosa Rorug01G0335600.1 Rorug01G0335700 Rorug01G0335800 Rorug01G0335800 Rorug01G0335800 Rorug01G0335800 Rorug01G0335900 Rorug01G0335900 Rorug01G0336000 Rorug01G0336000 Rorug05G0114700 Rorug05G0114800
rosa_samantha Rh1AG342300 Rh1AG342500 Rh1AG342700 Rh1BG302700 Rh1BG303000 Rh1BG303100 Rh1BG303400 Rh1BG303500 Rh1BG303800 Rh1BG304100 Rh1CG319200 Rh1CG319400 Rh1CG319800 Rh1CG319900 Rh1DG335300 Rh1DG335500 Rh1DG335900 Rh1DG336200 Rh2AG106400 Rh3DG307700 Rh4CG281300 Rh5AG206700 Rh5CG227400 Rh5DG209100 Rh7CG338300
rosa_wichuraiana Rw1G030420 Rw1G030430 Rw1G030460 Rw1G030470 Rw1G030480 Rw1G030510 Rw1G030520 Rw5G018800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 740
Acc36I ACCTGC 1 cut(s) 740
AciI CCGC 3 cut(s) 53, 149, 180
AclWI GGATC 3 cut(s) 580, 593, 652
AcoI YGGCCR 2 cut(s) 177, 911
AcsI RAATTY 4 cut(s) 417, 506, 981, 1215
AcuI CTGAAG 2 cut(s) 1103, 1213
AfaI GTAC 1 cut(s) 1010
AfiI CCNNNNNNNGG 5 cut(s) 138, 175, 347, 496, 1077
AgsI TTSAA 6 cut(s) 551, 572, 673, 699, 938, 1191
AjnI CCWGG 1 cut(s) 245
AleI CACNNNNGTG 1 cut(s) 580
AloI GAACNNNNNNTCC 4 cut(s) 150, 182, 879, 911
AluBI AGCT 8 cut(s) 525, 803, 1025, 1037, 1117, 1209, 1227, 1251
AluI AGCT 8 cut(s) 525, 803, 1025, 1037, 1117, 1209, 1227, 1251
Alw26I GTCTC 1 cut(s) 1156
AlwI GGATC 3 cut(s) 580, 593, 652
Ama87I CYCGRG 1 cut(s) 1070
AoxI GGCC 7 cut(s) 20, 26, 177, 911, 942, 1003, 1079
ApeKI GCWGC 9 cut(s) 111, 436, 614, 800, 1019, 1022, 1034, 1114, 1248
ApoI RAATTY 4 cut(s) 417, 506, 981, 1215
AspA2I CCTAGG 2 cut(s) 23, 427
AspLEI GCGC 1 cut(s) 1145
AspS9I GGNCC 4 cut(s) 20, 27, 743, 942
AsuC2I CCSGG 2 cut(s) 342, 919
AsuHPI GGTGA 4 cut(s) 287, 764, 790, 1142
AvaI CYCGRG 1 cut(s) 1070
AvaII GGWCC 1 cut(s) 743
AvrII CCTAGG 2 cut(s) 23, 427
BamHI GGATCC 1 cut(s) 585
BbsI GAAGAC 1 cut(s) 41
BbvI GCAGC 8 cut(s) 123, 423, 601, 812, 1006, 1034, 1046, 1126
BccI CCATC 3 cut(s) 38, 669, 775
BceAI ACGGC 1 cut(s) 136
BciT130I CCWGG 1 cut(s) 247
BclI TGATCA 1 cut(s) 1221
BcnI CCSGG 2 cut(s) 342, 919
BcoDI GTCTC 1 cut(s) 1156
BfaI CTAG 5 cut(s) 24, 428, 626, 906, 932
BfmI CTRYAG 2 cut(s) 819, 1020
BfuAI ACCTGC 1 cut(s) 740
BglII AGATCT 1 cut(s) 928
BlnI CCTAGG 2 cut(s) 23, 427
BmcAI AGTACT 1 cut(s) 1010
Bme1390I CCNGG 3 cut(s) 247, 342, 919
Bme18I GGWCC 1 cut(s) 743
BmeT110I CYCGRG 1 cut(s) 1070
BmgT120I GGNCC 4 cut(s) 20, 27, 743, 942
BmiI GGNNCC 1 cut(s) 587
BmrFI CCNGG 3 cut(s) 247, 342, 919
BmsI GCATC 2 cut(s) 448, 979
BoxI GACNNNNGTC 1 cut(s) 189
BpiI GAAGAC 1 cut(s) 41
BpmI CTGGAG 2 cut(s) 789, 1231
Bpu10I CCTNAGC 1 cut(s) 735
BpuEI CTTGAG 1 cut(s) 28
BpuMI CCSGG 2 cut(s) 342, 919
BsaJI CCNNGG 5 cut(s) 23, 427, 607, 618, 918
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 5 cut(s) 138, 175, 347, 496, 1077
Bse118I RCCGGY 3 cut(s) 138, 489, 909
Bse1I ACTGG 3 cut(s) 696, 893, 1006
BseBI CCWGG 1 cut(s) 247
BseDI CCNNGG 5 cut(s) 23, 427, 607, 618, 918
BseGI GGATG 3 cut(s) 256, 442, 487
BseLI CCNNNNNNNGG 5 cut(s) 138, 175, 347, 496, 1077
BseNI ACTGG 3 cut(s) 696, 893, 1006
BseXI GCAGC 8 cut(s) 123, 423, 601, 812, 1006, 1034, 1046, 1126
BshFI GGCC 7 cut(s) 22, 28, 179, 913, 944, 1005, 1081
BsiHKCI CYCGRG 1 cut(s) 1070
BsiSI CCGG 6 cut(s) 139, 169, 342, 490, 910, 918
BslFI GGGAC 2 cut(s) 680, 799
BslI CCNNNNNNNGG 5 cut(s) 138, 175, 347, 496, 1077
BsmAI GTCTC 1 cut(s) 1156
BsmFI GGGAC 2 cut(s) 680, 799
BsnI GGCC 7 cut(s) 22, 28, 179, 913, 944, 1005, 1081
BsoBI CYCGRG 1 cut(s) 1070
Bsp143I GATC 6 cut(s) 13, 585, 657, 928, 1202, 1221
BspACI CCGC 3 cut(s) 53, 149, 180
BspANI GGCC 7 cut(s) 22, 28, 179, 913, 944, 1005, 1081
BspLI GGNNCC 1 cut(s) 587
BspMAI CTGCAG 1 cut(s) 1024
BspMI ACCTGC 1 cut(s) 740
BspPI GGATC 3 cut(s) 580, 593, 652
BsrFI RCCGGY 3 cut(s) 138, 489, 909
BsrI ACTGG 3 cut(s) 696, 893, 1006
BssAI RCCGGY 3 cut(s) 138, 489, 909
BssECI CCNNGG 5 cut(s) 23, 427, 607, 618, 918
BssMI GATC 6 cut(s) 13, 585, 657, 928, 1202, 1221
BssT1I CCWWGG 4 cut(s) 23, 427, 607, 618
Bst2UI CCWGG 1 cut(s) 247
Bst4CI ACNGT 2 cut(s) 122, 644
BstC8I GCNNGC 2 cut(s) 911, 961
BstDEI CTNAG 1 cut(s) 735
BstF5I GGATG 3 cut(s) 256, 442, 487
BstHHI GCGC 1 cut(s) 1145
BstKTI GATC 6 cut(s) 16, 588, 660, 931, 1205, 1224
BstMAI GTCTC 1 cut(s) 1156
BstMBI GATC 6 cut(s) 13, 585, 657, 928, 1202, 1221
BstMWI GCNNNNNNNGC 5 cut(s) 148, 611, 877, 1031, 1043
BstNI CCWGG 1 cut(s) 247
BstNSI RCATGY 1 cut(s) 355
BstPAI GACNNNNGTC 1 cut(s) 189
BstSCI CCNGG 3 cut(s) 245, 340, 917
BstSFI CTRYAG 2 cut(s) 819, 1020
BstV1I GCAGC 8 cut(s) 123, 423, 601, 812, 1006, 1034, 1046, 1126
BstV2I GAAGAC 1 cut(s) 41
BstX2I RGATCY 2 cut(s) 585, 928
BstXI CCANNNNNNTGG 1 cut(s) 582
BstYI RGATCY 2 cut(s) 585, 928
BsuRI GGCC 7 cut(s) 22, 28, 179, 913, 944, 1005, 1081
BtsCI GGATG 3 cut(s) 256, 442, 487
BtsI GCAGTG 1 cut(s) 1201
BtsIMutI CAGTG 2 cut(s) 649, 1201
BveI ACCTGC 1 cut(s) 740
Cac8I GCNNGC 2 cut(s) 911, 961
CfoI GCGC 1 cut(s) 1145
Cfr10I RCCGGY 3 cut(s) 138, 489, 909
Cfr13I GGNCC 4 cut(s) 20, 27, 743, 942
CseI GACGC 1 cut(s) 106
Csp6I GTAC 1 cut(s) 1009
CviAII CATG 4 cut(s) 107, 352, 764, 1087
CviQI GTAC 1 cut(s) 1009
DdeI CTNAG 1 cut(s) 735
DpnI GATC 6 cut(s) 15, 587, 659, 930, 1204, 1223
DpnII GATC 6 cut(s) 13, 585, 657, 928, 1202, 1221
DraI TTTAAA 1 cut(s) 226
EaeI YGGCCR 2 cut(s) 177, 911
Eco130I CCWWGG 4 cut(s) 23, 427, 607, 618
Eco47I GGWCC 1 cut(s) 743
Eco57I CTGAAG 2 cut(s) 1103, 1213
Eco88I CYCGRG 1 cut(s) 1070
EcoRII CCWGG 1 cut(s) 245
EcoT14I CCWWGG 4 cut(s) 23, 427, 607, 618
ErhI CCWWGG 4 cut(s) 23, 427, 607, 618
FaeI CATG 4 cut(s) 110, 355, 767, 1090
FaqI GGGAC 2 cut(s) 680, 799
FatI CATG 4 cut(s) 106, 351, 763, 1086
FauNDI CATATG 1 cut(s) 790
FbaI TGATCA 1 cut(s) 1221
FokI GGATG 3 cut(s) 243, 429, 494
FspBI CTAG 5 cut(s) 24, 428, 626, 906, 932
GlaI GCGC 1 cut(s) 1144
GsuI CTGGAG 2 cut(s) 789, 1231
HaeIII GGCC 7 cut(s) 22, 28, 179, 913, 944, 1005, 1081
HapII CCGG 6 cut(s) 139, 169, 342, 490, 910, 918
HgaI GACGC 1 cut(s) 106
HhaI GCGC 1 cut(s) 1145
Hin1II CATG 4 cut(s) 110, 355, 767, 1090
Hin6I GCGC 1 cut(s) 1143
HinP1I GCGC 1 cut(s) 1143
HincII GTYRAC 1 cut(s) 411
HindII GTYRAC 1 cut(s) 411
HindIII AAGCTT 1 cut(s) 523
HinfI GANTC 5 cut(s) 128, 216, 695, 1124, 1149
HpaII CCGG 6 cut(s) 139, 169, 342, 490, 910, 918
HphI GGTGA 4 cut(s) 287, 764, 790, 1142
Hpy166II GTNNAC 2 cut(s) 389, 411
Hpy188I TCNGA 4 cut(s) 157, 721, 860, 1202
Hpy188III TCNNGA 5 cut(s) 260, 665, 806, 899, 1121
Hpy8I GTNNAC 2 cut(s) 389, 411
Hpy99I CGWCG 1 cut(s) 100
HpyAV CCTTC 4 cut(s) 272, 377, 426, 578
HpyCH4III ACNGT 2 cut(s) 122, 644
HpyCH4V TGCA 4 cut(s) 439, 800, 1022, 1046
HpyF10VI GCNNNNNNNGC 5 cut(s) 148, 611, 877, 1031, 1043
HpyF3I CTNAG 1 cut(s) 735
Hsp92II CATG 4 cut(s) 110, 355, 767, 1090
HspAI GCGC 1 cut(s) 1143
KroI GCCGGC 1 cut(s) 909
KroNI GCCGGC 1 cut(s) 911
Ksp22I TGATCA 1 cut(s) 1221
Kzo9I GATC 6 cut(s) 13, 585, 657, 928, 1202, 1221
LmnI GCTCC 1 cut(s) 808
Lsp1109I GCAGC 8 cut(s) 123, 423, 601, 812, 1006, 1034, 1046, 1126
LweI GCATC 2 cut(s) 448, 979
MaeI CTAG 5 cut(s) 24, 428, 626, 906, 932
MaeIII GTNAC 2 cut(s) 395, 1130
MalI GATC 6 cut(s) 15, 587, 659, 930, 1204, 1223
MboI GATC 6 cut(s) 13, 585, 657, 928, 1202, 1221
MboII GAAGA 8 cut(s) 41, 50, 60, 92, 211, 225, 379, 610
MflI RGATCY 2 cut(s) 585, 928
MlyI GAGTC 2 cut(s) 122, 1158
MmeI TCCRAC 2 cut(s) 156, 287
MnlI CCTC 5 cut(s) 472, 640, 1071, 1087, 1132
MroNI GCCGGC 1 cut(s) 909
MseI TTAA 8 cut(s) 92, 225, 300, 356, 375, 830, 978, 999
MslI CAYNNNNRTG 4 cut(s) 123, 447, 580, 642
MspI CCGG 6 cut(s) 139, 169, 342, 490, 910, 918
MspR9I CCNGG 3 cut(s) 247, 342, 919
MvaI CCWGG 1 cut(s) 247
MwoI GCNNNNNNNGC 5 cut(s) 148, 611, 877, 1031, 1043
NaeI GCCGGC 1 cut(s) 911
NciI CCSGG 2 cut(s) 342, 919
NdeI CATATG 1 cut(s) 790
NdeII GATC 6 cut(s) 13, 585, 657, 928, 1202, 1221
NgoMIV GCCGGC 1 cut(s) 909
NlaIII CATG 4 cut(s) 110, 355, 767, 1090
NlaIV GGNNCC 1 cut(s) 587
NmuCI GTSAC 2 cut(s) 395, 1130
NspI RCATGY 1 cut(s) 355
OliI CACNNNNGTG 1 cut(s) 580
PaqCI CACCTGC 1 cut(s) 740
PdiI GCCGGC 1 cut(s) 911
PfeI GAWTC 3 cut(s) 216, 695, 1124
PleI GAGTC 2 cut(s) 122, 1157
PpsI GAGTC 2 cut(s) 122, 1157
PshAI GACNNNNGTC 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 245
PspGI CCWGG 1 cut(s) 245
PspN4I GGNNCC 1 cut(s) 587
PspPI GGNCC 4 cut(s) 20, 27, 743, 942
PstI CTGCAG 1 cut(s) 1024
PsuI RGATCY 2 cut(s) 585, 928
RsaI GTAC 1 cut(s) 1010
RsaNI GTAC 1 cut(s) 1009
RseI CAYNNNNRTG 4 cut(s) 123, 447, 580, 642
SaqAI TTAA 8 cut(s) 92, 225, 300, 356, 375, 830, 978, 999
Sau3AI GATC 6 cut(s) 13, 585, 657, 928, 1202, 1221
Sau96I GGNCC 4 cut(s) 20, 27, 743, 942
ScaI AGTACT 1 cut(s) 1010
SchI GAGTC 2 cut(s) 122, 1158
ScrFI CCNGG 3 cut(s) 247, 342, 919
SfaNI GCATC 2 cut(s) 448, 979
SfcI CTRYAG 2 cut(s) 819, 1020
SinI GGWCC 1 cut(s) 743
SmiMI CAYNNNNRTG 4 cut(s) 123, 447, 580, 642
SmlI CTYRAG 1 cut(s) 7
SmoI CTYRAG 1 cut(s) 7
SsiI CCGC 3 cut(s) 53, 149, 180
SspMI CTAG 5 cut(s) 24, 428, 626, 906, 932
StyD4I CCNGG 3 cut(s) 245, 340, 917
StyI CCWWGG 4 cut(s) 23, 427, 607, 618
TaaI ACNGT 2 cut(s) 122, 644
TaqI TCGA 4 cut(s) 38, 477, 770, 873
TatI WGTACW 1 cut(s) 1008
TauI GCSGC 1 cut(s) 182
TfiI GAWTC 3 cut(s) 216, 695, 1124
Tru1I TTAA 8 cut(s) 92, 225, 300, 356, 375, 830, 978, 999
Tru9I TTAA 8 cut(s) 92, 225, 300, 356, 375, 830, 978, 999
TscAI CASTG 2 cut(s) 649, 1201
TseFI GTSAC 2 cut(s) 395, 1130
TseI GCWGC 9 cut(s) 111, 436, 614, 800, 1019, 1022, 1034, 1114, 1248
Tsp45I GTSAC 2 cut(s) 395, 1130
TspDTI ATGAA 8 cut(s) 60, 95, 245, 519, 524, 611, 626, 1103
TspGWI ACGGA 2 cut(s) 53, 756
TspRI CASTG 2 cut(s) 649, 1201
VpaK11BI GGWCC 1 cut(s) 743
XapI RAATTY 4 cut(s) 417, 506, 981, 1215
XceI RCATGY 1 cut(s) 355
XmaJI CCTAGG 2 cut(s) 23, 427
XspI CTAG 5 cut(s) 24, 428, 626, 906, 932
ZrmI AGTACT 1 cut(s) 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.