Rroxscaffold_4G00289380
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000305
Physical Location & Seq
Reverse (-)
10015814 .. 10021925
6112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00289380.1

Sequence Viewer

Length: 1722 bp
ATGAATGATTCAAGTGGTTTATTCATCTCATACAAAGAGGTATTTATAGGAAAACATTTGAAGTGTGAGAGCTCACAGAGGATTGATAGTATGAGTGGTGGGATTCGTACAAGCGTGAAGGGGTACCTTATTGCCAAACGCCTGGCTTTCACTGGGGAAATTGATACAGTGCATGAGGTGAATGAGACCATCAATAGCAATTTGACTGGGCCGCAAGCTTCTGATGATTTCTTAGATAAGAGACCACAAGATCATCCGGGGACGAGTCGTGCTTCAACTTCGTATGGGCTCAGTATAGGTGATGATAGCATTCTTCACTTGCCAATGTTGAGCAATGCTGCAAAGGTGTCATATGCAGCAGTGTCTTCTTGTCCTCGGACAGAAGGGGAGATTTTGCTAAGGAGATTCTTCTTGAATGAACTTAAAATGGCGACCTGGAACTTCCATCCTGATAATATGGTGGGCGAAGGTGGTTTTGGTTCTGTTTTCAAGGGGTGGGTTGATGACTCATCAACAGCTGCCAACCCTGGTAAGGGCATGTTGATTGCTGTGAAGAGGATTAACAAAGAAGGTTTCTTGGGTCACGAGCAATGGTTGGCTGAAATTTACTACCTAGGAAGGCTGCGTCATCCAAATCTTGTGAAATTGTTGGGTTATTGCTTTGAAGATGGTCATCGGCTTCTGACTTACAATGCCAAACTCGCTGACTTTGGTTTAGCAAAGGATGGACCCGCAGGTGATAAAAGCCATGTTTCAACAGCAGTGATGGGGACACATGGGTATGCTGCTCCTGAGTATATTGCTACAGGTGTCTCTGGTTTACAGTTCTTTTCTTCTTCTTTGATTAACCATGTAAACTGCTACTTACTTGGTTTACCAGAAACTATGACAAGTTATTATGCCTTTCTGATAATTGTTTATGTTGAAGGTCATTTAACTGCCAAATGTGATGTATATGGGTTTGGAGTTGTGATGCTCGAAATGCTGTCTGGAAGACGTAGTCTGGACCAAAGCCTGCCGACTGTGCAACAGAATTTAGTCGACTGGGCCAAACCTTACCTCGCCAGCAAACGCAGAGTTCTCAAAGTTTTTGATCCTCGTTTAAAAGGCCAGTACTCTGTGGCTGGAGCTCTTAAAGCAGCTAATCTTGCAAAACAATGCTTATCAGAAGAACCTGAGTTTAGGCCAAACATGACTGAGGTGGTAAAAGCATTGGAGCAGCTTCAGGAACCTGAAGACATGAAGGGTTTGAAGATTCCTCAAAATGAGCGTTGCCAGAGTGCTCGTCATTTAACCAGCAAAAATGATGTGTATGGTTTTGGAGTTGTTATGCTTGAATTGCTGACAGGAAAACCAGTTCTTGACACTAGCCGGTCAACCAGGGAACAGGATCTAGTTGAATGGTCCAAACCATACCTTGCCAGCAAACGCAGAGTTCTTAAAATTTTTGATGCTCGTATTAAAGGCCAGTACTCTGTGGCTGCAGCTCGTAAAGCGGCTAATCTTGCAAATCAATGCTTATCAACAGAACCCGAGTTCAGGCCAAACATGAATGAGGTGGTAAAAGCATTGGAGCAGCTTCAGGAATCAGGTGACATTGAGGGCGCAGGAGTCCCTCGTCACGAGCCTCGCCGATCTCATTCCAATTCAAACAGTGCCAAATCAAGCTGGAGAAGTAGGATCAGCTTCAGGTCATCTGCTTCTTGTAGCTACATAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0035821 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0046777 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0060089 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0075136 GO:0080134 GO:0098542 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

573

Amino Acids

63.49

Weight (kDa)

8.82

Isoelectric Point (pI)

43.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 149 - 228 5.1e-06 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 151 - 227 6.1e-08 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 427 - 522 6.6e-06 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000305)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39660 AT3G55450 AT3G55450
fragaria_vesca FvH4_7g22540 FvH4_7g22540 FvH4_7g22540 FvH4_7g22540 FvH4_7g22550 FvH4_7g22550 FvH4_7g22590 FvH4_7g22592 FvH4_7g22592 FvH4_7g22593 FvH4_7g22593 FvH4_7g22593
malus_domestica MD01G1134700.v1.1 MD07G1199400.v1.1
prunus_persica Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1
pyrus_communis pycom01g15540 pycom07g18630
rosa_chinensis RchiOBHm_Chr1g0367221 RchiOBHm_Chr1g0367231 RchiOBHm_Chr1g0367251 RchiOBHm_Chr1g0367261 RchiOBHm_Chr1g0367311 RchiOBHm_Chr1g0367321 RchiOBHm_Chr1g0367351 RchiOBHm_Chr1g0367401 RchiOBHm_Chr1g0367411 RchiOBHm_Chr1g0367581 RchiOBHm_Chr5g0029531
rosa_laevigata RLG00000001400 RLG00000024338 RLG00000027271 RLG00000027274 RLG00000027277 RLG00000027278 RLG00000027280 RLG00000027281 RLG00000027282 RLG00000027285 RLG00000027286 RLG00000027288 RLG00000033176
rosa_multiflora Rmu_sc0002690.1_g000002 Rmu_sc0004181.1_g000007 Rmu_sc0008470.1_g000013 Rmu_sc0008470.1_g000015 Rmu_sc0009775.1_g000001 Rmu_sc0009775.1_g000003 Rmu_sc0009775.1_g000004 Rmu_sc0009775.1_g000005 Rmu_sc0011000.1_g000008 Rmu_sc0014291.1_g000001 Rmu_sc0014291.1_g000005 Rmu_sc0014291.1_g000008
rosa_roxburghii Rroxscaffold_1G00050440 Rroxscaffold_4G00289340 Rroxscaffold_4G00289350 Rroxscaffold_4G00289380 Rroxscaffold_4G00289400 Rroxscaffold_4G00289410 Rroxscaffold_4G00289450 Rroxscaffold_4G00289490 Rroxscaffold_4G00289500 Rroxscaffold_4G00289510 Rroxscaffold_6G00420130 Rroxscaffold_6G00421370
rosa_rugosa Rorug01G0335600.1 Rorug01G0335700 Rorug01G0335800 Rorug01G0335800 Rorug01G0335800 Rorug01G0335800 Rorug01G0335900 Rorug01G0335900 Rorug01G0336000 Rorug01G0336000 Rorug05G0114700 Rorug05G0114800
rosa_samantha Rh1AG342300 Rh1AG342500 Rh1AG342700 Rh1BG302700 Rh1BG303000 Rh1BG303100 Rh1BG303400 Rh1BG303500 Rh1BG303800 Rh1BG304100 Rh1CG319200 Rh1CG319400 Rh1CG319800 Rh1CG319900 Rh1DG335300 Rh1DG335500 Rh1DG335900 Rh1DG336200 Rh2AG106400 Rh3DG307700 Rh4CG281300 Rh5AG206700 Rh5CG227400 Rh5DG209100 Rh7CG338300
rosa_wichuraiana Rw1G030420 Rw1G030430 Rw1G030460 Rw1G030470 Rw1G030480 Rw1G030510 Rw1G030520 Rw5G018800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 725
Acc36I ACCTGC 1 cut(s) 725
Acc65I GGTACC 1 cut(s) 123
AccB1I GGYRCC 1 cut(s) 123
AccI GTMKAC 1 cut(s) 1041
AciI CCGC 3 cut(s) 212, 732, 1496
AclWI GGATC 3 cut(s) 1088, 1398, 1688
AcsI RAATTY 3 cut(s) 603, 1033, 1443
AcuI CTGAAG 4 cut(s) 1208, 1254, 1565, 1672
AfaI GTAC 4 cut(s) 109, 125, 1115, 1472
AfiI CCNNNNNNNGG 3 cut(s) 532, 533, 1539
AjnI CCWGG 4 cut(s) 141, 434, 526, 1379
AjuI GAANNNNNNNTTGG 2 cut(s) 459, 491
AloI GAACNNNNNNTCC 2 cut(s) 1341, 1373
Alw21I GWGCWC 3 cut(s) 74, 1132, 1285
Alw26I GTCTC 3 cut(s) 179, 235, 817
AlwI GGATC 3 cut(s) 1088, 1398, 1688
Ama87I CYCGRG 1 cut(s) 1532
AoxI GGCC 6 cut(s) 209, 1047, 1108, 1184, 1465, 1541
ApoI RAATTY 3 cut(s) 603, 1033, 1443
Asp718I GGTACC 1 cut(s) 123
AspA2I CCTAGG 1 cut(s) 613
AspLEI GCGC 1 cut(s) 1607
AspS9I GGNCC 5 cut(s) 209, 728, 1006, 1047, 1404
AsuC2I CCSGG 1 cut(s) 258
AsuHPI GGTGA 4 cut(s) 190, 311, 749, 1604
AvaI CYCGRG 1 cut(s) 1532
AvaII GGWCC 3 cut(s) 728, 1006, 1404
AvrII CCTAGG 1 cut(s) 613
BanI GGYRCC 1 cut(s) 123
BanII GRGCYC 3 cut(s) 74, 291, 1132
BauI CACGAG 2 cut(s) 584, 1622
BbsI GAAGAC 3 cut(s) 357, 1000, 1242
Bbv12I GWGCWC 3 cut(s) 74, 1132, 1285
BccI CCATC 5 cut(s) 197, 453, 662, 719, 760
BciT130I CCWGG 4 cut(s) 143, 436, 528, 1381
BcnI CCSGG 1 cut(s) 258
BcoDI GTCTC 3 cut(s) 179, 235, 817
BfaI CTAG 3 cut(s) 614, 1368, 1394
BfmI CTRYAG 2 cut(s) 804, 1482
BfuAI ACCTGC 1 cut(s) 725
BlnI CCTAGG 1 cut(s) 613
BmcAI AGTACT 2 cut(s) 1115, 1472
Bme1390I CCNGG 5 cut(s) 143, 258, 436, 528, 1381
Bme18I GGWCC 3 cut(s) 728, 1006, 1404
BmeT110I CYCGRG 1 cut(s) 1532
BmgT120I GGNCC 5 cut(s) 209, 728, 1006, 1047, 1404
BmiI GGNNCC 3 cut(s) 125, 730, 1230
BmrFI CCNGG 5 cut(s) 143, 258, 436, 528, 1381
BmrI ACTGGG 3 cut(s) 162, 216, 1054
BmsI GCATC 2 cut(s) 963, 1441
BmuI ACTGGG 3 cut(s) 162, 216, 1054
BpiI GAAGAC 3 cut(s) 357, 1000, 1242
BpmI CTGGAG 2 cut(s) 1146, 1690
Bpu10I CCTNAGC 1 cut(s) 398
BpuMI CCSGG 1 cut(s) 258
BsaBI GATNNNNATC 1 cut(s) 672
BsaI GGTCTC 2 cut(s) 179, 235
BsaJI CCNNGG 5 cut(s) 257, 374, 526, 613, 1380
Bsc4I CCNNNNNNNGG 3 cut(s) 532, 533, 1539
Bse118I RCCGGY 1 cut(s) 1371
Bse1I ACTGG 6 cut(s) 157, 211, 1049, 1111, 1355, 1468
Bse3DI GCAATG 2 cut(s) 340, 596
Bse8I GATNNNNATC 1 cut(s) 672
BseBI CCWGG 4 cut(s) 143, 436, 528, 1381
BseDI CCNNGG 5 cut(s) 257, 374, 526, 613, 1380
BseGI GGATG 4 cut(s) 253, 445, 628, 730
BseJI GATNNNNATC 1 cut(s) 672
BseLI CCNNNNNNNGG 3 cut(s) 532, 533, 1539
BseMI GCAATG 2 cut(s) 340, 596
BseMII CTCAG 4 cut(s) 304, 783, 1167, 1188
BseNI ACTGG 6 cut(s) 157, 211, 1049, 1111, 1355, 1468
BshFI GGCC 6 cut(s) 211, 1049, 1110, 1186, 1467, 1543
BshNI GGYRCC 1 cut(s) 123
BsiHKAI GWGCWC 3 cut(s) 74, 1132, 1285
BsiHKCI CYCGRG 1 cut(s) 1532
BsiSI CCGG 2 cut(s) 257, 1372
BslFI GGGAC 3 cut(s) 274, 784, 1598
BslI CCNNNNNNNGG 3 cut(s) 532, 533, 1539
BsmAI GTCTC 3 cut(s) 179, 235, 817
BsmFI GGGAC 3 cut(s) 274, 784, 1598
BsmI GAATGC 1 cut(s) 309
BsnI GGCC 6 cut(s) 211, 1049, 1110, 1186, 1467, 1543
Bso31I GGTCTC 2 cut(s) 179, 235
BsoBI CYCGRG 1 cut(s) 1532
Bsp1286I GDGCHC 4 cut(s) 74, 291, 1132, 1285
Bsp143I GATC 5 cut(s) 250, 1093, 1390, 1634, 1680
BspACI CCGC 3 cut(s) 212, 732, 1496
BspANI GGCC 6 cut(s) 211, 1049, 1110, 1186, 1467, 1543
BspCNI CTCAG 4 cut(s) 303, 784, 1168, 1189
BspLI GGNNCC 3 cut(s) 125, 730, 1230
BspMAI CTGCAG 1 cut(s) 1486
BspMI ACCTGC 1 cut(s) 725
BspPI GGATC 3 cut(s) 1088, 1398, 1688
BspT107I GGYRCC 1 cut(s) 123
BspTNI GGTCTC 2 cut(s) 179, 235
BsrDI GCAATG 2 cut(s) 340, 596
BsrFI RCCGGY 1 cut(s) 1371
BsrI ACTGG 6 cut(s) 157, 211, 1049, 1111, 1355, 1468
BssAI RCCGGY 1 cut(s) 1371
BssECI CCNNGG 5 cut(s) 257, 374, 526, 613, 1380
BssMI GATC 5 cut(s) 250, 1093, 1390, 1634, 1680
BssSI CACGAG 2 cut(s) 584, 1622
BssT1I CCWWGG 1 cut(s) 613
Bst2BI CACGAG 2 cut(s) 584, 1622
Bst2UI CCWGG 4 cut(s) 143, 436, 528, 1381
Bst4CI ACNGT 4 cut(s) 169, 825, 1024, 1655
Bst6I CTCTTC 1 cut(s) 548
BstC8I GCNNGC 4 cut(s) 216, 1016, 1066, 1423
BstDEI CTNAG 6 cut(s) 232, 290, 398, 792, 1176, 1197
BstF5I GGATG 4 cut(s) 253, 445, 628, 730
BstHHI GCGC 1 cut(s) 1607
BstKTI GATC 5 cut(s) 253, 1096, 1393, 1637, 1683
BstMAI GTCTC 3 cut(s) 179, 235, 817
BstMBI GATC 5 cut(s) 250, 1093, 1390, 1634, 1680
BstMWI GCNNNNNNNGC 8 cut(s) 701, 982, 1024, 1136, 1148, 1339, 1493, 1505
BstNI CCWGG 4 cut(s) 143, 436, 528, 1381
BstNSI RCATGY 1 cut(s) 541
BstSCI CCNGG 5 cut(s) 141, 256, 434, 526, 1379
BstSFI CTRYAG 2 cut(s) 804, 1482
BstV2I GAAGAC 3 cut(s) 357, 1000, 1242
BstX2I RGATCY 1 cut(s) 1390
BstXI CCANNNNNNTGG 1 cut(s) 142
BstYI RGATCY 1 cut(s) 1390
BsuRI GGCC 6 cut(s) 211, 1049, 1110, 1186, 1467, 1543
BtsCI GGATG 4 cut(s) 253, 445, 628, 730
BtsI GCAGTG 2 cut(s) 366, 768
BtsIMutI CAGTG 5 cut(s) 150, 174, 366, 768, 1660
BveI ACCTGC 1 cut(s) 725
Cac8I GCNNGC 4 cut(s) 216, 1016, 1066, 1423
CfoI GCGC 1 cut(s) 1607
Cfr10I RCCGGY 1 cut(s) 1371
Cfr13I GGNCC 5 cut(s) 209, 728, 1006, 1047, 1404
CseI GACGC 1 cut(s) 614
Csp6I GTAC 4 cut(s) 108, 124, 1114, 1471
CviAII CATG 8 cut(s) 173, 538, 749, 776, 851, 1192, 1240, 1549
CviQI GTAC 4 cut(s) 108, 124, 1114, 1471
DdeI CTNAG 6 cut(s) 232, 290, 398, 792, 1176, 1197
DpnI GATC 5 cut(s) 252, 1095, 1392, 1636, 1682
DpnII GATC 5 cut(s) 250, 1093, 1390, 1634, 1680
DraI TTTAAA 1 cut(s) 1104
Eam1104I CTCTTC 1 cut(s) 548
EarI CTCTTC 1 cut(s) 548
Ecl136II GAGCTC 2 cut(s) 72, 1130
Eco130I CCWWGG 1 cut(s) 613
Eco24I GRGCYC 3 cut(s) 74, 291, 1132
Eco31I GGTCTC 2 cut(s) 179, 235
Eco47I GGWCC 3 cut(s) 728, 1006, 1404
Eco53kI GAGCTC 2 cut(s) 72, 1130
Eco57I CTGAAG 4 cut(s) 1208, 1254, 1565, 1672
Eco88I CYCGRG 1 cut(s) 1532
EcoICRI GAGCTC 2 cut(s) 72, 1130
EcoRII CCWGG 4 cut(s) 141, 434, 526, 1379
EcoT14I CCWWGG 1 cut(s) 613
EcoT38I GRGCYC 3 cut(s) 74, 291, 1132
ErhI CCWWGG 1 cut(s) 613
FaeI CATG 8 cut(s) 176, 541, 752, 779, 854, 1195, 1243, 1552
FaqI GGGAC 3 cut(s) 274, 784, 1598
FatI CATG 8 cut(s) 172, 537, 748, 775, 850, 1191, 1239, 1548
FauI CCCGC 1 cut(s) 739
FauNDI CATATG 1 cut(s) 352
FblI GTMKAC 1 cut(s) 1041
FokI GGATG 4 cut(s) 240, 432, 615, 737
FriOI GRGCYC 3 cut(s) 74, 291, 1132
FspBI CTAG 3 cut(s) 614, 1368, 1394
GlaI GCGC 1 cut(s) 1606
GsuI CTGGAG 2 cut(s) 1146, 1690
HaeIII GGCC 6 cut(s) 211, 1049, 1110, 1186, 1467, 1543
HapII CCGG 2 cut(s) 257, 1372
HgaI GACGC 1 cut(s) 614
HhaI GCGC 1 cut(s) 1607
Hin1II CATG 8 cut(s) 176, 541, 752, 779, 854, 1195, 1243, 1552
Hin6I GCGC 1 cut(s) 1605
HinP1I GCGC 1 cut(s) 1605
HincII GTYRAC 2 cut(s) 1042, 1377
HindII GTYRAC 2 cut(s) 1042, 1377
HindIII AAGCTT 1 cut(s) 216
HinfI GANTC 8 cut(s) 8, 103, 265, 405, 506, 1255, 1586, 1611
HpaII CCGG 2 cut(s) 257, 1372
HphI GGTGA 4 cut(s) 190, 311, 749, 1604
Hpy166II GTNNAC 5 cut(s) 821, 856, 875, 1042, 1377
Hpy188I TCNGA 5 cut(s) 223, 378, 684, 909, 1168
Hpy8I GTNNAC 5 cut(s) 821, 856, 875, 1042, 1377
HpyAV CCTTC 7 cut(s) 112, 377, 461, 563, 612, 920, 1237
HpyCH4III ACNGT 4 cut(s) 169, 825, 1024, 1655
HpyCH4IV ACGT 1 cut(s) 997
HpyCH4V TGCA 7 cut(s) 172, 341, 356, 1027, 1151, 1484, 1508
HpyF10VI GCNNNNNNNGC 8 cut(s) 701, 982, 1024, 1136, 1148, 1339, 1493, 1505
HpyF3I CTNAG 6 cut(s) 232, 290, 398, 792, 1176, 1197
HpySE526I ACGT 1 cut(s) 997
Hsp92II CATG 8 cut(s) 176, 541, 752, 779, 854, 1195, 1243, 1552
HspAI GCGC 1 cut(s) 1605
KpnI GGTACC 1 cut(s) 127
Kzo9I GATC 5 cut(s) 250, 1093, 1390, 1634, 1680
LmnI GCTCC 4 cut(s) 793, 1127, 1216, 1573
LweI GCATC 2 cut(s) 963, 1441
MaeI CTAG 3 cut(s) 614, 1368, 1394
MaeII ACGT 1 cut(s) 997
MaeIII GTNAC 3 cut(s) 581, 1592, 1619
MalI GATC 5 cut(s) 252, 1095, 1392, 1636, 1682
MboI GATC 5 cut(s) 250, 1093, 1390, 1634, 1680
MflI RGATCY 1 cut(s) 1390
MhlI GDGCHC 4 cut(s) 74, 291, 1132, 1285
MluCI AATT 9 cut(s) 159, 199, 603, 644, 912, 1033, 1337, 1443, 1645
MlyI GAGTC 3 cut(s) 274, 500, 1620
MseI TTAA 9 cut(s) 423, 561, 846, 935, 1103, 1134, 1292, 1440, 1461
MslI CAYNNNNRTG 1 cut(s) 780
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 2 cut(s) 257, 1372
MspR9I CCNGG 5 cut(s) 143, 258, 436, 528, 1381
Mva1269I GAATGC 1 cut(s) 309
MvaI CCWGG 4 cut(s) 143, 436, 528, 1381
MwoI GCNNNNNNNGC 8 cut(s) 701, 982, 1024, 1136, 1148, 1339, 1493, 1505
NciI CCSGG 1 cut(s) 258
NdeI CATATG 1 cut(s) 352
NdeII GATC 5 cut(s) 250, 1093, 1390, 1634, 1680
NlaIII CATG 8 cut(s) 176, 541, 752, 779, 854, 1195, 1243, 1552
NlaIV GGNNCC 3 cut(s) 125, 730, 1230
NmuCI GTSAC 3 cut(s) 581, 1592, 1619
NspI RCATGY 1 cut(s) 541
PaqCI CACCTGC 1 cut(s) 725
PctI GAATGC 1 cut(s) 309
PfeI GAWTC 5 cut(s) 8, 103, 405, 1255, 1586
PflFI GACNNNGTC 1 cut(s) 999
PleI GAGTC 3 cut(s) 273, 500, 1619
PpsI GAGTC 3 cut(s) 273, 500, 1619
Psp124BI GAGCTC 2 cut(s) 74, 1132
Psp6I CCWGG 4 cut(s) 141, 434, 526, 1379
PspGI CCWGG 4 cut(s) 141, 434, 526, 1379
PspN4I GGNNCC 3 cut(s) 125, 730, 1230
PspPI GGNCC 5 cut(s) 209, 728, 1006, 1047, 1404
PstI CTGCAG 1 cut(s) 1486
PsuI RGATCY 1 cut(s) 1390
PsyI GACNNNGTC 1 cut(s) 999
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 4 cut(s) 109, 125, 1115, 1472
RsaNI GTAC 4 cut(s) 108, 124, 1114, 1471
RseI CAYNNNNRTG 1 cut(s) 780
SacI GAGCTC 2 cut(s) 74, 1132
SalI GTCGAC 1 cut(s) 1040
SaqAI TTAA 9 cut(s) 423, 561, 846, 935, 1103, 1134, 1292, 1440, 1461
Sau3AI GATC 5 cut(s) 250, 1093, 1390, 1634, 1680
Sau96I GGNCC 5 cut(s) 209, 728, 1006, 1047, 1404
ScaI AGTACT 2 cut(s) 1115, 1472
SchI GAGTC 3 cut(s) 274, 500, 1620
ScrFI CCNGG 5 cut(s) 143, 258, 436, 528, 1381
SduI GDGCHC 4 cut(s) 74, 291, 1132, 1285
SfaNI GCATC 2 cut(s) 963, 1441
SfcI CTRYAG 2 cut(s) 804, 1482
SinI GGWCC 3 cut(s) 728, 1006, 1404
SmiMI CAYNNNNRTG 1 cut(s) 780
Sse9I AATT 9 cut(s) 159, 199, 603, 644, 912, 1033, 1337, 1443, 1645
SsiI CCGC 3 cut(s) 212, 732, 1496
SspMI CTAG 3 cut(s) 614, 1368, 1394
SstI GAGCTC 2 cut(s) 74, 1132
StyD4I CCNGG 5 cut(s) 141, 256, 434, 526, 1379
StyI CCWWGG 1 cut(s) 613
TaaI ACNGT 4 cut(s) 169, 825, 1024, 1655
TaiI ACGT 1 cut(s) 1000
TaqI TCGA 2 cut(s) 978, 1041
TasI AATT 9 cut(s) 159, 199, 603, 644, 912, 1033, 1337, 1443, 1645
TatI WGTACW 2 cut(s) 1113, 1470
TauI GCSGC 2 cut(s) 214, 1499
TfiI GAWTC 5 cut(s) 8, 103, 405, 1255, 1586
Tru1I TTAA 9 cut(s) 423, 561, 846, 935, 1103, 1134, 1292, 1440, 1461
Tru9I TTAA 9 cut(s) 423, 561, 846, 935, 1103, 1134, 1292, 1440, 1461
TscAI CASTG 5 cut(s) 157, 174, 366, 768, 1660
TseFI GTSAC 3 cut(s) 581, 1592, 1619
Tsp45I GTSAC 3 cut(s) 581, 1592, 1619
TspDTI ATGAA 5 cut(s) 13, 17, 432, 1256, 1565
TspRI CASTG 5 cut(s) 157, 174, 366, 768, 1660
Tth111I GACNNNGTC 1 cut(s) 999
VpaK11BI GGWCC 3 cut(s) 728, 1006, 1404
XapI RAATTY 3 cut(s) 603, 1033, 1443
XceI RCATGY 1 cut(s) 541
XmaJI CCTAGG 1 cut(s) 613
XmiI GTMKAC 1 cut(s) 1041
XspI CTAG 3 cut(s) 614, 1368, 1394
ZrmI AGTACT 2 cut(s) 1115, 1472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.