RLG00000027285
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000305
Physical Location & Seq
Reverse (-)
10296719 .. 10311320
14602 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027285

Sequence Viewer

Length: 1284 bp
ATGATGTTACCAACTCTCTCTCCTCCTCAGCTTTCCAAGAGGTCTCTCTGTCTCTGGTTAACTCCTTCACTTGAAGAACTGAATCAGCTTCAGTACTCAGGAAAACAAGAGACGAGCACGGACTTGGATTCAACAAAGTCATATCGGCTCAATAGGTTTAGTGGTAGCATTCGTTGCTTGCCAATATTGAGCTTTGCTTCAAAGAGCAGGGTTTCAAATAGAACTAGAATCAGAGCTGAGAGTCTGCCTCACAAAGTGCCTTCAACTCCTCGGACAGAGGTTGAGATCTTGCTAAACAGCTTCTTTTTTAATGAACTGAGAACCGCCACCAGGAACTTTCGGCCTGATAATGTGGTGGGTGAGGGTGGTTTTGGTACTGTCTTTAAGGGGTGGATTGATCAGAATTCATTAGCTGCTGCCAAGCCTGGTGAAGGAATGGCTGTTGCTATAAAGAGGCTTAACCAAGAAGGTTATCAGGGTCGGGAGGAATGGTTTAAAGAAATTTACTACCTTGGACAGCTGCGTCACCCAAATCTTGTGAAGTTGATAGGCTATTGTTCAGAGGATAACGAACTGCTTCTGGTGTATGAATTTGTGCCCCGTGGCAGTTTGGAGAATCATATATTTAGAAGGGCTTCTTACTTTCAACCACTTTCATGGTCCCTTCGTATGAAGATTGCCCTTGGTGCTGCTAAGGCTCTAGCATGTCTTCACAATGAAGCAGAAGTTATATACCGTGACTTTAAAACATCTAATATCCTGTTGGATTCAACCTACAATGCCAAGCTCGCTGATTTTGGTTTGGTCGTGGATGCACGAGCATTTGACAGACACCGAGGTGCCAAAACAACGATCATGGGCACGTACGGATATTCAGCTCCCGAATATACAGCTACAGGTCGTTTGACCACCAAATGTGATGTGTATGGTTTTGGAGTTGTTATGCTCGAATTGGTTTCTGGAAAACGAGTTTTTGACCAGAACCGGCCACCGGGGGAACAGAACTTAGTTGAATGGGCCAAACCTTACCTTGTCAGCAACCACAGAGTCCTCCAAATTTTTGATCATCATATCAAAGGCCAGTTTTCTGTGGCCAGAGCTCTTAAAGCAGTTGACCTTGCATTTCTTTGCCTATCAAGAGATCCCAAGCTTAGGCCAAACATGAATAATGTGGTAAAAACATTGGAGCAGCTTCAGGCATCCAGTGACATGGACGGCTTGGGGATCTCTCAAAATCAATGTCGCCAAAATCCACATGTCAGTTCAATCAATGGTCCCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0035821 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0046777 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0060089 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0075136 GO:0080134 GO:0098542 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

428

Amino Acids

48.19

Weight (kDa)

9.36

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 116 - 393 2.2e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 117 - 395 8.1e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000305)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39660 AT3G55450 AT3G55450
fragaria_vesca FvH4_7g22540 FvH4_7g22540 FvH4_7g22540 FvH4_7g22540 FvH4_7g22550 FvH4_7g22550 FvH4_7g22590 FvH4_7g22592 FvH4_7g22592 FvH4_7g22593 FvH4_7g22593 FvH4_7g22593
malus_domestica MD01G1134700.v1.1 MD07G1199400.v1.1
prunus_persica Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1 Prupe.2G236100_v2.0.a1
pyrus_communis pycom01g15540 pycom07g18630
rosa_chinensis RchiOBHm_Chr1g0367221 RchiOBHm_Chr1g0367231 RchiOBHm_Chr1g0367251 RchiOBHm_Chr1g0367261 RchiOBHm_Chr1g0367311 RchiOBHm_Chr1g0367321 RchiOBHm_Chr1g0367351 RchiOBHm_Chr1g0367401 RchiOBHm_Chr1g0367411 RchiOBHm_Chr1g0367581 RchiOBHm_Chr5g0029531
rosa_laevigata RLG00000001400 RLG00000024338 RLG00000027271 RLG00000027274 RLG00000027277 RLG00000027278 RLG00000027280 RLG00000027281 RLG00000027282 RLG00000027285 RLG00000027286 RLG00000027288 RLG00000033176
rosa_multiflora Rmu_sc0002690.1_g000002 Rmu_sc0004181.1_g000007 Rmu_sc0008470.1_g000013 Rmu_sc0008470.1_g000015 Rmu_sc0009775.1_g000001 Rmu_sc0009775.1_g000003 Rmu_sc0009775.1_g000004 Rmu_sc0009775.1_g000005 Rmu_sc0011000.1_g000008 Rmu_sc0014291.1_g000001 Rmu_sc0014291.1_g000005 Rmu_sc0014291.1_g000008
rosa_roxburghii Rroxscaffold_1G00050440 Rroxscaffold_4G00289340 Rroxscaffold_4G00289350 Rroxscaffold_4G00289380 Rroxscaffold_4G00289400 Rroxscaffold_4G00289410 Rroxscaffold_4G00289450 Rroxscaffold_4G00289490 Rroxscaffold_4G00289500 Rroxscaffold_4G00289510 Rroxscaffold_6G00420130 Rroxscaffold_6G00421370
rosa_rugosa Rorug01G0335600.1 Rorug01G0335700 Rorug01G0335800 Rorug01G0335800 Rorug01G0335800 Rorug01G0335800 Rorug01G0335900 Rorug01G0335900 Rorug01G0336000 Rorug01G0336000 Rorug05G0114700 Rorug05G0114800
rosa_samantha Rh1AG342300 Rh1AG342500 Rh1AG342700 Rh1BG302700 Rh1BG303000 Rh1BG303100 Rh1BG303400 Rh1BG303500 Rh1BG303800 Rh1BG304100 Rh1CG319200 Rh1CG319400 Rh1CG319800 Rh1CG319900 Rh1DG335300 Rh1DG335500 Rh1DG335900 Rh1DG336200 Rh2AG106400 Rh3DG307700 Rh4CG281300 Rh5AG206700 Rh5CG227400 Rh5DG209100 Rh7CG338300
rosa_wichuraiana Rw1G030420 Rw1G030430 Rw1G030460 Rw1G030470 Rw1G030480 Rw1G030510 Rw1G030520 Rw5G018800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 522
AccB1I GGYRCC 1 cut(s) 839
AciI CCGC 1 cut(s) 324
AclWI GGATC 2 cut(s) 1136, 1232
AcoI YGGCCR 2 cut(s) 986, 1092
AcsI RAATTY 4 cut(s) 403, 501, 590, 1056
AcuI CTGAAG 2 cut(s) 74, 1178
AdeI CACNNNGTG 1 cut(s) 256
AfaI GTAC 3 cut(s) 95, 376, 866
AfiI CCNNNNNNNGG 4 cut(s) 330, 431, 991, 1152
AflIII ACRYGT 1 cut(s) 1255
AgsI TTSAA 9 cut(s) 74, 132, 201, 216, 264, 647, 771, 1013, 1266
AjnI CCWGG 2 cut(s) 329, 424
AleI CACNNNNGTG 1 cut(s) 837
Alw21I GWGCWC 2 cut(s) 119, 1102
Alw26I GTCTC 3 cut(s) 48, 56, 104
AlwI GGATC 2 cut(s) 1136, 1232
AoxI GGCC 6 cut(s) 341, 986, 1017, 1078, 1092, 1154
ApeKI GCWGC 5 cut(s) 413, 416, 520, 689, 1189
ApoI RAATTY 4 cut(s) 403, 501, 590, 1056
ArsI GACNNNNNNTTYG 2 cut(s) 1225, 1257
Asp700I GAANNNNTTC 2 cut(s) 576, 634
AspS9I GGNCC 3 cut(s) 660, 1017, 1274
AsuC2I CCSGG 1 cut(s) 993
AsuHPI GGTGA 3 cut(s) 371, 440, 518
AvaII GGWCC 2 cut(s) 660, 1274
BaeGI GKGCMC 2 cut(s) 600, 863
BalI TGGCCA 1 cut(s) 1094
BanI GGYRCC 1 cut(s) 839
BanII GRGCYC 1 cut(s) 1102
BauI CACGAG 1 cut(s) 816
BbsI GAAGAC 1 cut(s) 701
Bbv12I GWGCWC 2 cut(s) 119, 1102
BbvCI CCTCAGC 1 cut(s) 27
BbvI GCAGC 5 cut(s) 400, 403, 507, 676, 1201
BceAI ACGGC 1 cut(s) 1231
BciT130I CCWGG 2 cut(s) 331, 426
BclI TGATCA 2 cut(s) 397, 1063
BcnI CCSGG 1 cut(s) 993
BcoDI GTCTC 3 cut(s) 48, 56, 104
BfaI CTAG 2 cut(s) 225, 701
BfmI CTRYAG 1 cut(s) 894
BglII AGATCT 1 cut(s) 285
BisI GCNGC 5 cut(s) 414, 417, 521, 690, 1190
BlsI GCNGC 5 cut(s) 415, 418, 522, 691, 1191
BmcAI AGTACT 1 cut(s) 95
Bme1390I CCNGG 3 cut(s) 331, 426, 993
Bme18I GGWCC 2 cut(s) 660, 1274
BmgT120I GGNCC 3 cut(s) 660, 1017, 1274
BmiI GGNNCC 3 cut(s) 662, 841, 1276
BmrFI CCNGG 3 cut(s) 331, 426, 993
BmsI GCATC 2 cut(s) 802, 1208
BpiI GAAGAC 1 cut(s) 701
BplI GAGNNNNNCTC 2 cut(s) 232, 264
Bpu10I CCTNAGC 3 cut(s) 27, 693, 1151
BpuMI CCSGG 1 cut(s) 993
BsaAI YACGTR 1 cut(s) 864
BsaI GGTCTC 1 cut(s) 48
BsaJI CCNNGG 6 cut(s) 269, 511, 601, 682, 835, 992
BsaXI ACNNNNNCTCC 3 cut(s) 34, 476, 506
Bsc4I CCNNNNNNNGG 4 cut(s) 330, 431, 991, 1152
Bse118I RCCGGY 1 cut(s) 984
Bse1I ACTGG 2 cut(s) 1081, 1203
BseBI CCWGG 2 cut(s) 331, 426
BseDI CCNNGG 6 cut(s) 269, 511, 601, 682, 835, 992
BseGI GGATG 2 cut(s) 817, 1199
BseLI CCNNNNNNNGG 4 cut(s) 330, 431, 991, 1152
BseMII CTCAG 4 cut(s) 41, 111, 228, 308
BseNI ACTGG 2 cut(s) 1081, 1203
BseRI GAGGAG 3 cut(s) 12, 15, 258
BseSI GKGCMC 2 cut(s) 600, 863
BseXI GCAGC 5 cut(s) 400, 403, 507, 676, 1201
BshFI GGCC 6 cut(s) 343, 988, 1019, 1080, 1094, 1156
BshNI GGYRCC 1 cut(s) 839
BsiHKAI GWGCWC 2 cut(s) 119, 1102
BsiSI CCGG 2 cut(s) 985, 992
BsiWI CGTACG 1 cut(s) 864
BslFI GGGAC 2 cut(s) 646, 1260
BslI CCNNNNNNNGG 4 cut(s) 330, 431, 991, 1152
BsmAI GTCTC 3 cut(s) 48, 56, 104
BsmBI CGTCTC 1 cut(s) 104
BsmFI GGGAC 2 cut(s) 646, 1260
BsmI GAATGC 1 cut(s) 168
BsnI GGCC 6 cut(s) 343, 988, 1019, 1080, 1094, 1156
Bso31I GGTCTC 1 cut(s) 48
Bsp1286I GDGCHC 4 cut(s) 119, 600, 863, 1102
Bsp143I GATC 6 cut(s) 285, 397, 852, 1063, 1141, 1224
BspACI CCGC 1 cut(s) 324
BspANI GGCC 6 cut(s) 343, 988, 1019, 1080, 1094, 1156
BspCNI CTCAG 4 cut(s) 40, 110, 229, 309
BspLI GGNNCC 3 cut(s) 662, 841, 1276
BspPI GGATC 2 cut(s) 1136, 1232
BspT107I GGYRCC 1 cut(s) 839
BspTNI GGTCTC 1 cut(s) 48
BsrFI RCCGGY 1 cut(s) 984
BsrI ACTGG 2 cut(s) 1081, 1203
BssAI RCCGGY 1 cut(s) 984
BssECI CCNNGG 6 cut(s) 269, 511, 601, 682, 835, 992
BssMI GATC 6 cut(s) 285, 397, 852, 1063, 1141, 1224
BssSI CACGAG 1 cut(s) 816
BssT1I CCWWGG 2 cut(s) 511, 682
Bst2BI CACGAG 1 cut(s) 816
Bst2UI CCWGG 2 cut(s) 331, 426
Bst4CI ACNGT 2 cut(s) 379, 737
BstAPI GCANNNNNTGC 1 cut(s) 174
BstBAI YACGTR 1 cut(s) 864
BstC8I GCNNGC 2 cut(s) 179, 789
BstDEI CTNAG 7 cut(s) 27, 97, 237, 317, 693, 1006, 1151
BstDSI CCRYGG 1 cut(s) 601
BstENI CCTNNNNNAGG 1 cut(s) 429
BstF5I GGATG 2 cut(s) 817, 1199
BstKTI GATC 6 cut(s) 288, 400, 855, 1066, 1144, 1227
BstMAI GTCTC 3 cut(s) 48, 56, 104
BstMBI GATC 6 cut(s) 285, 397, 852, 1063, 1141, 1224
BstMWI GCNNNNNNNGC 5 cut(s) 174, 686, 695, 788, 1106
BstNI CCWGG 2 cut(s) 331, 426
BstNSI RCATGY 2 cut(s) 708, 1259
BstSCI CCNGG 3 cut(s) 329, 424, 991
BstSFI CTRYAG 1 cut(s) 894
BstSLI GKGCMC 2 cut(s) 600, 863
BstV1I GCAGC 5 cut(s) 400, 403, 507, 676, 1201
BstV2I GAAGAC 1 cut(s) 701
BstX2I RGATCY 3 cut(s) 285, 1141, 1224
BstXI CCANNNNNNTGG 2 cut(s) 657, 1210
BstYI RGATCY 3 cut(s) 285, 1141, 1224
BsuRI GGCC 6 cut(s) 343, 988, 1019, 1080, 1094, 1156
BtgI CCRYGG 1 cut(s) 601
BtsCI GGATG 2 cut(s) 817, 1199
BtsIMutI CAGTG 1 cut(s) 1210
Cac8I GCNNGC 2 cut(s) 179, 789
Cfr10I RCCGGY 1 cut(s) 984
Cfr13I GGNCC 3 cut(s) 660, 1017, 1274
CseI GACGC 1 cut(s) 512
Csp6I GTAC 3 cut(s) 94, 375, 865
CviAII CATG 6 cut(s) 657, 705, 856, 1162, 1210, 1256
CviQI GTAC 3 cut(s) 94, 375, 865
DdeI CTNAG 7 cut(s) 27, 97, 237, 317, 693, 1006, 1151
DpnI GATC 6 cut(s) 287, 399, 854, 1065, 1143, 1226
DpnII GATC 6 cut(s) 285, 397, 852, 1063, 1141, 1224
DraI TTTAAA 2 cut(s) 496, 745
DraIII CACNNNGTG 1 cut(s) 256
DrdI GACNNNNNNGTC 1 cut(s) 522
DseDI GACNNNNNNGTC 1 cut(s) 522
EaeI YGGCCR 2 cut(s) 986, 1092
Ecl136II GAGCTC 1 cut(s) 1100
Eco130I CCWWGG 2 cut(s) 511, 682
Eco24I GRGCYC 1 cut(s) 1102
Eco31I GGTCTC 1 cut(s) 48
Eco47I GGWCC 2 cut(s) 660, 1274
Eco53kI GAGCTC 1 cut(s) 1100
Eco57I CTGAAG 2 cut(s) 74, 1178
EcoICRI GAGCTC 1 cut(s) 1100
EcoNI CCTNNNNNAGG 1 cut(s) 429
EcoRI GAATTC 1 cut(s) 403
EcoRII CCWGG 2 cut(s) 329, 424
EcoT14I CCWWGG 2 cut(s) 511, 682
EcoT38I GRGCYC 1 cut(s) 1102
ErhI CCWWGG 2 cut(s) 511, 682
Esp3I CGTCTC 1 cut(s) 104
FaeI CATG 6 cut(s) 660, 708, 859, 1165, 1213, 1259
FalI AAGNNNNNCTT 2 cut(s) 622, 654
FaqI GGGAC 2 cut(s) 646, 1260
FatI CATG 6 cut(s) 656, 704, 855, 1161, 1209, 1255
FbaI TGATCA 2 cut(s) 397, 1063
Fnu4HI GCNGC 5 cut(s) 414, 417, 521, 690, 1190
FokI GGATG 2 cut(s) 824, 1186
FriOI GRGCYC 1 cut(s) 1102
Fsp4HI GCNGC 5 cut(s) 414, 417, 521, 690, 1190
FspBI CTAG 2 cut(s) 225, 701
GluI GCNGC 5 cut(s) 414, 417, 521, 690, 1190
HaeIII GGCC 6 cut(s) 343, 988, 1019, 1080, 1094, 1156
HapII CCGG 2 cut(s) 985, 992
HgaI GACGC 1 cut(s) 512
Hin1II CATG 6 cut(s) 660, 708, 859, 1165, 1213, 1259
HincII GTYRAC 2 cut(s) 60, 1114
HindII GTYRAC 2 cut(s) 60, 1114
HindIII AAGCTT 1 cut(s) 1148
HinfI GANTC 7 cut(s) 82, 128, 228, 241, 616, 767, 1047
HpaI GTTAAC 1 cut(s) 60
HpaII CCGG 2 cut(s) 985, 992
HphI GGTGA 3 cut(s) 371, 440, 518
Hpy166II GTNNAC 2 cut(s) 60, 1114
Hpy188I TCNGA 4 cut(s) 233, 273, 402, 562
Hpy188III TCNNGA 5 cut(s) 99, 482, 881, 960, 1137
Hpy8I GTNNAC 2 cut(s) 60, 1114
HpyAV CCTTC 6 cut(s) 75, 270, 425, 461, 624, 674
HpyCH4III ACNGT 2 cut(s) 379, 737
HpyCH4IV ACGT 1 cut(s) 863
HpyCH4V TGCA 2 cut(s) 815, 1121
HpyF10VI GCNNNNNNNGC 5 cut(s) 174, 686, 695, 788, 1106
HpyF3I CTNAG 7 cut(s) 27, 97, 237, 317, 693, 1006, 1151
HpySE526I ACGT 1 cut(s) 863
Hsp92II CATG 6 cut(s) 660, 708, 859, 1165, 1213, 1259
Ksp22I TGATCA 2 cut(s) 397, 1063
KspAI GTTAAC 1 cut(s) 60
Kzo9I GATC 6 cut(s) 285, 397, 852, 1063, 1141, 1224
LmnI GCTCC 2 cut(s) 883, 1186
Lsp1109I GCAGC 5 cut(s) 400, 403, 507, 676, 1201
LweI GCATC 2 cut(s) 802, 1208
MaeI CTAG 2 cut(s) 225, 701
MaeII ACGT 1 cut(s) 863
MaeIII GTNAC 4 cut(s) 6, 524, 737, 1205
MalI GATC 6 cut(s) 287, 399, 854, 1065, 1143, 1226
MboI GATC 6 cut(s) 285, 397, 852, 1063, 1141, 1224
MboII GAAGA 3 cut(s) 86, 685, 701
MflI RGATCY 3 cut(s) 285, 1141, 1224
MhlI GDGCHC 4 cut(s) 119, 600, 863, 1102
MlsI TGGCCA 1 cut(s) 1094
MluCI AATT 5 cut(s) 403, 501, 590, 950, 1056
MluNI TGGCCA 1 cut(s) 1094
MlyI GAGTC 2 cut(s) 250, 1056
MmeI TCCRAC 1 cut(s) 744
Mox20I TGGCCA 1 cut(s) 1094
MroXI GAANNNNTTC 2 cut(s) 576, 634
MscI TGGCCA 1 cut(s) 1094
MseI TTAA 7 cut(s) 59, 309, 384, 459, 495, 744, 1104
MslI CAYNNNNRTG 2 cut(s) 655, 837
Msp20I TGGCCA 1 cut(s) 1094
MspA1I CMGCKG 1 cut(s) 520
MspI CCGG 2 cut(s) 985, 992
MspR9I CCNGG 3 cut(s) 331, 426, 993
Mva1269I GAATGC 1 cut(s) 168
MvaI CCWGG 2 cut(s) 331, 426
MwoI GCNNNNNNNGC 5 cut(s) 174, 686, 695, 788, 1106
NciI CCSGG 1 cut(s) 993
NdeII GATC 6 cut(s) 285, 397, 852, 1063, 1141, 1224
NlaIII CATG 6 cut(s) 660, 708, 859, 1165, 1213, 1259
NlaIV GGNNCC 3 cut(s) 662, 841, 1276
NmuCI GTSAC 3 cut(s) 524, 737, 1205
NspI RCATGY 2 cut(s) 708, 1259
OliI CACNNNNGTG 1 cut(s) 837
PciI ACATGT 1 cut(s) 1255
PctI GAATGC 1 cut(s) 168
PdmI GAANNNNTTC 2 cut(s) 576, 634
PfeI GAWTC 5 cut(s) 82, 128, 228, 616, 767
Pfl23II CGTACG 1 cut(s) 864
PkrI GCNGC 5 cut(s) 415, 418, 522, 691, 1191
PleI GAGTC 2 cut(s) 249, 1055
PpsI GAGTC 2 cut(s) 249, 1055
Ppu21I YACGTR 1 cut(s) 864
PscI ACATGT 1 cut(s) 1255
Psp124BI GAGCTC 1 cut(s) 1102
Psp6I CCWGG 2 cut(s) 329, 424
PspGI CCWGG 2 cut(s) 329, 424
PspLI CGTACG 1 cut(s) 864
PspN4I GGNNCC 3 cut(s) 662, 841, 1276
PspPI GGNCC 3 cut(s) 660, 1017, 1274
PsuI RGATCY 3 cut(s) 285, 1141, 1224
PvuII CAGCTG 1 cut(s) 520
RsaI GTAC 3 cut(s) 95, 376, 866
RsaNI GTAC 3 cut(s) 94, 375, 865
RseI CAYNNNNRTG 2 cut(s) 655, 837
SacI GAGCTC 1 cut(s) 1102
SaqAI TTAA 7 cut(s) 59, 309, 384, 459, 495, 744, 1104
SatI GCNGC 5 cut(s) 414, 417, 521, 690, 1190
Sau3AI GATC 6 cut(s) 285, 397, 852, 1063, 1141, 1224
Sau96I GGNCC 3 cut(s) 660, 1017, 1274
ScaI AGTACT 1 cut(s) 95
SchI GAGTC 2 cut(s) 250, 1056
ScrFI CCNGG 3 cut(s) 331, 426, 993
SduI GDGCHC 4 cut(s) 119, 600, 863, 1102
SfaNI GCATC 2 cut(s) 802, 1208
SfcI CTRYAG 1 cut(s) 894
SinI GGWCC 2 cut(s) 660, 1274
SmiMI CAYNNNNRTG 2 cut(s) 655, 837
Sse9I AATT 5 cut(s) 403, 501, 590, 950, 1056
SsiI CCGC 1 cut(s) 324
SspI AATATT 1 cut(s) 186
SspMI CTAG 2 cut(s) 225, 701
SstI GAGCTC 1 cut(s) 1102
StyD4I CCNGG 3 cut(s) 329, 424, 991
StyI CCWWGG 2 cut(s) 511, 682
TaaI ACNGT 2 cut(s) 379, 737
TaiI ACGT 1 cut(s) 866
TaqI TCGA 1 cut(s) 948
TasI AATT 5 cut(s) 403, 501, 590, 950, 1056
TatI WGTACW 1 cut(s) 93
TfiI GAWTC 5 cut(s) 82, 128, 228, 616, 767
Tru1I TTAA 7 cut(s) 59, 309, 384, 459, 495, 744, 1104
Tru9I TTAA 7 cut(s) 59, 309, 384, 459, 495, 744, 1104
TscAI CASTG 1 cut(s) 1210
TseFI GTSAC 3 cut(s) 524, 737, 1205
TseI GCWGC 5 cut(s) 413, 416, 520, 689, 1189
Tsp45I GTSAC 3 cut(s) 524, 737, 1205
TspDTI ATGAA 7 cut(s) 327, 396, 603, 645, 686, 732, 1178
TspGWI ACGGA 2 cut(s) 134, 882
TspRI CASTG 1 cut(s) 1210
VpaK11BI GGWCC 2 cut(s) 660, 1274
XagI CCTNNNNNAGG 1 cut(s) 429
XapI RAATTY 4 cut(s) 403, 501, 590, 1056
XceI RCATGY 2 cut(s) 708, 1259
XmnI GAANNNNTTC 2 cut(s) 576, 634
XspI CTAG 2 cut(s) 225, 701
ZrmI AGTACT 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.