Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000225

Physical Location & Seq

Genomic Coordinates Reverse (-)

14529803 .. 14535437

Gene Structure 5635 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1070800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1977 bp

ATGGCTTTCGTAGTGAAGGTTGAGGAATCGAGGCCAGCTGCCGACGAGAGGCCATCCGCTGGGCCGGTGTATCGGAGCATTTACGCTAAAGATGGTCTCATGGAGTTGCCGGAGGGGTTGGAATCTCCTTGGCAGTTTTTAAGTGTCTCTGCTAAGAAGTATCCAAACAACCCAATGCTTGGGCGGCGCGAAGTCACAGACAAAAAGGTGGGTCCCTATGTGTGGCTTACGTACCAAGAAGTTTATGATTCTGCCATTCGAATTGGTTCAGCCATCAGAAGCCGGGGTGTTAATCCTGGGGATCGCTGCGGTATATATGGATCAAACTGCCCCCGGTGGCTTACTGCAATGGAGGCCTGTAATAGCCAAGCCATAACATATGTTCCACTCTATGACACGCTTGGTGCAAATGCAGTTGAGTTCATCATCAATCATGCTGAAGTTTCAATAGCTTTTGTTCAAGACAACAAACTCCCTTCTGTTCTGTCATGCCTTCCAAATTGTTCTACCAATTTTAAAACAATTGTCAGTTTTGAAAATGTTTCTAGCACTCAAAAGGAGGAAGCTGAGGGACTAGGGGTATCTTGTTTTTCTTGGGAGGAATTCTCTCAGTTGGGAAATGCAGATTGTGAACTACCTCCGAAACGGAAGACTGACATTTGCACTATAATGTATACAAGTGGGACAACTGGAGAACCAAAAGGTGTAATTATTAGTAACAGAGCAATTCTGGCAGAAGTATTGTCTGTGGAGCAACTACTTTTTCTAACAGATAGAGTGTGTACAGAAGAAGATTCATATTTTTCCTTTCTTCCATTGGCCCATATATATGACCAAATAATGGAGAGCTATTGCATCTACAAAGGATCCTCAGTAGGCTTCTGGCGAGGAGATATCAGATTTCTACTCGAGGACCTTCAGGAACTAAGGCCTACTATGTTTTGTGGGGTACCTAGAGTGTATGATCGCATATACTCTGGTATTGTTAACAAAGTTTCATCTGGGGGTGTATTGAAGAAGACATTATTCCAATATGCTTATAACTACAAGTTGGCGAACCTGGAAAAGGGTCTCCCACAAGAAAAAGCAGCACCTCTCTTGGACAAACTTGTCTTTGATAAGATAAAACAAGTATTGGGGGGACGACTTCGTCTATTGTTGTCTGGTGCTGCACCCTTGCCTAGGCATGTGGAGGAATTTTTGAGGGTCACCAGCTGCAGCACTTTATCCCAAGGATATGGCCTTACTGAAAGCTGTGGTGGCTGCTTCACGTCCATTAGCAATGTTTTTCCTATGATCGGAACTGTCGGTGTCCCTGTCACAACTATTGAAGCAAGGCTTGAGTCAGTGCCGGCAATGGGATACGATGCACTTTCCAGTGTACCCCGTGGAGAAATTTGCCTGAGAGGAACAACCTTGTTTTCTGGTTACCATAAGCGAGAAGATCTAACAGAGGAAGTCCTTATTGATGGGTGGTTTCATACAGGTGACATTGGAGAATGGCAGGCAGATGGATCAATGAAAATCATAGATAGGAAAAAAAATATATATAAACTATCGCAAGGTGAATATGTTGCCGTGGAAAACATTGAAAGCAAATACTTGCAATGTCCTCTTATCACATCGATTTGGGTTTATGGGAACAGCTTCGAGTCATTTCTTGTTGCTGTGGTGGTCCCTGACAGTAAGGCAATGGAGCATTGGGCAGCAGAGTATCACTTGACTGATGATTTCAAATCATTATGTCAAAACTCGAAGGCAAGAAAGTACATTTTGGATGAGCTTAATAGTACTGGTCAGAAACAGCATCTTCGAGGTTTTGAGCTGTTAAAAGCAGTTCACTTGGAACCGAATCCCTTTGACATGGAGAGGGATCTCATAACTCCAACATTCAAACTGAAGAGACCACAGCTGCTTAAATATTACAAGGACTGTATCGATAAACTTTACAGTGAAGGAAAGGGAGCAAGGCCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001676 GO:0003674 GO:0003824 GO:0004467 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005911 GO:0006082 GO:0006629 GO:0006631 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009791 GO:0009987 GO:0010015 GO:0010101 GO:0010102 GO:0010143 GO:0010311 GO:0012505 GO:0015645 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0019752 GO:0022622 GO:0030054 GO:0031957 GO:0032501 GO:0032502 GO:0032787 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048527 GO:0048528 GO:0048646 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0071704 GO:0090696 GO:0090697 GO:0090698 GO:0098542 GO:0099402 GO:1901576 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko00061 ko00071 ko01100 ko01212 ko02024 ko03320 ko04146 ko04216 ko04714 ko04920 map00061 map00071 map01100 map01212 map02024 map03320 map04146 map04216 map04714 map04920

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

659

Amino Acids

73.69

Weight (kDa)

5.8

Isoelectric Point (pI)

42.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	50 - 478	5.7e-81	AMP-binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0000225)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49430 AT1G49430
fragaria_vesca	FvH4_2g07450 FvH4_2g07450 FvH4_2g07450 FvH4_6g05950 FvH4_6g05960
malus_domestica	MD05G1070800.v1.1 MD10G1085200.v1.1 MD10G1085400.v1.1
prunus_persica	Prupe.8G108400_v2.0.a1 Prupe.8G108600_v2.0.a1 Prupe.8G108900_v2.0.a1
pyrus_communis	pycom10g06680
rosa_chinensis	RchiOBHm_Chr0c38g0502991 RchiOBHm_Chr0c38g0503001 RchiOBHm_Chr0c38g0503011 RchiOBHm_Chr0c38g0503031 RchiOBHm_Chr1g0351481 RchiOBHm_Chr6g0261071 RchiOBHm_Chr6g0261081 RchiOBHm_Chr6g0261101 RchiOBHm_Chr6g0261171 RchiOBHm_Chr6g0261181 RchiOBHm_Chr6g0261201 RchiOBHm_Chr6g0261211 RchiOBHm_Chr6g0261271 RchiOBHm_Chr6g0261291 RchiOBHm_Chr6g0261301 RchiOBHm_Chr6g0261311 RchiOBHm_Chr6g0261431 RchiOBHm_Chr6g0261521 RchiOBHm_Chr6g0261591 RchiOBHm_Chr6g0261601 RchiOBHm_Chr6g0261611 RchiOBHm_Chr6g0261621 RchiOBHm_Chr6g0261631 RchiOBHm_Chr6g0261651 RchiOBHm_Chr6g0261671 RchiOBHm_Chr6g0261761 RchiOBHm_Chr6g0261791 RchiOBHm_Chr6g0261801 RchiOBHm_Chr6g0261891 RchiOBHm_Chr6g0261911 RchiOBHm_Chr6g0261921 RchiOBHm_Chr6g0261931 RchiOBHm_Chr6g0261951 RchiOBHm_Chr6g0261961 RchiOBHm_Chr6g0261981 RchiOBHm_Chr6g0262641 RchiOBHm_Chr6g0262661 RchiOBHm_Chr6g0262711 RchiOBHm_Chr6g0262721 RchiOBHm_Chr6g0262801 RchiOBHm_Chr6g0262811 RchiOBHm_Chr6g0262821 RchiOBHm_Chr6g0262831 RchiOBHm_Chr6g0262871 RchiOBHm_Chr6g0262881 RchiOBHm_Chr6g0267811
rosa_laevigata	RLG00000014207 RLG00000014410 RLG00000014461 RLG00000014462 RLG00000014463 RLG00000030683
rosa_multiflora	Rmu_co8313283.1_g000002 Rmu_sc0000596.1_g000052 Rmu_sc0001833.1_g000009 Rmu_sc0003487.1_g000003 Rmu_sc0003487.1_g000007 Rmu_sc0004766.1_g000001 Rmu_sc0005237.1_g000021 Rmu_sc0006655.1_g000001 Rmu_sc0015236.1_g000003 Rmu_sc0015236.1_g000004 Rmu_sc0028652.1_g000010 Rmu_sc0031142.1_g000001 Rmu_sc0036063.1_g000001 Rmu_ssc0000359.1_g000047 Rmu_ssc0000359.1_g000054 Rmu_ssc0000359.1_g000058 Rmu_ssc0000359.1_g000060
rosa_roxburghii	Rroxscaffold_175G00432240 Rroxscaffold_177G00434140 Rroxscaffold_177G00434220 Rroxscaffold_2G00103180 Rroxscaffold_2G00103450 Rroxscaffold_4G00314540 Rroxscaffold_7G00202920 Rroxscaffold_7G00202940 Rroxscaffold_7G00207640 Rroxscaffold_7G00207650 Rroxscaffold_7G00207670 Rroxscaffold_7G00207690 Rroxscaffold_7G00207700 Rroxscaffold_7G00207720
rosa_rugosa	Rorug05G0594700 Rorug05G0594900 Rorug06G0013300 Rorug07G0072100
rosa_samantha	Rh4AG177500 Rh5AG318000 Rh6AG114200 Rh6AG120600 Rh6AG137000 Rh6AG137100 Rh6AG161100 Rh6BG107900 Rh6BG108000 Rh6BG108200 Rh6BG108300 Rh6BG108400 Rh6BG113800 Rh6BG134400 Rh6BG134500 Rh6BG134600 Rh6BG134700 Rh6BG134900 Rh6BG135100 Rh6BG135300 Rh6BG135400 Rh6CG105000 Rh6CG105100 Rh6CG105500 Rh6CG105600 Rh6CG105700 Rh6CG105800 Rh6CG111800 Rh6CG132500 Rh6CG132900 Rh6CG133000 Rh6CG133200 Rh6CG133400 Rh6CG133500 Rh6CG133600 Rh6CG133700 Rh6CG133800 Rh6CG152600 Rh6CG159000 Rh6DG120200
rosa_wichuraiana	Rw0G012740 Rw6G002860 Rw6G009790 Rw6G010330

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1041
AasI	GACNNNNNNGTC	2 cut(s)	1109, 1149
Acc65I	GGTACC	1 cut(s)	949
AccB1I	GGYRCC	1 cut(s)	949
AccB7I	CCANNNNNTGG	3 cut(s)	59, 179, 841
AccI	GTMKAC	1 cut(s)	674
AccII	CGCG	1 cut(s)	189
AciI	CCGC	3 cut(s)	57, 184, 309
AclWI	GGATC	6 cut(s)	309, 328, 861, 874, 1522, 1881
AcsI	RAATTY	3 cut(s)	602, 1196, 1395
AcuI	CTGAAG	3 cut(s)	459, 902, 1919
AfaI	GTAC	6 cut(s)	233, 784, 951, 1383, 1769, 1792
AfiI	CCNNNNNNNGG	9 cut(s)	48, 59, 179, 222, 841, 1066, 1182, 1298, 1358
AgsI	TTSAA	8 cut(s)	447, 461, 536, 1015, 1331, 1592, 1735, 1894
AjiI	CACGTC	1 cut(s)	1272
AjnI	CCWGG	2 cut(s)	295, 1059
Alw26I	GTCTC	4 cut(s)	101, 151, 1076, 1897
AlwI	GGATC	6 cut(s)	309, 328, 861, 874, 1522, 1881
Ama87I	CYCGRG	1 cut(s)	908
AoxI	GGCC	8 cut(s)	32, 50, 62, 354, 819, 929, 1240, 1970
ApeKI	GCWGC	9 cut(s)	38, 306, 1088, 1169, 1215, 1218, 1263, 1706, 1912
ApoI	RAATTY	3 cut(s)	602, 1196, 1395
Asp700I	GAANNNNTTC	2 cut(s)	265, 1646
Asp718I	GGTACC	1 cut(s)	949
AspA2I	CCTAGG	1 cut(s)	1181
AspLEI	GCGC	1 cut(s)	189
AspS9I	GGNCC	5 cut(s)	62, 212, 820, 913, 1675
AsuC2I	CCSGG	2 cut(s)	284, 334
AsuHPI	GGTGA	3 cut(s)	1201, 1499, 1577
AsuII	TTCGAA	1 cut(s)	259
AvaI	CYCGRG	1 cut(s)	908
AvaII	GGWCC	3 cut(s)	212, 913, 1675
AvrII	CCTAGG	1 cut(s)	1181
BamHI	GGATCC	1 cut(s)	866
BanI	GGYRCC	1 cut(s)	949
BbsI	GAAGAC	2 cut(s)	656, 1025
BbvCI	CCTCAGC	1 cut(s)	567
BbvI	GCAGC	9 cut(s)	25, 293, 1100, 1156, 1202, 1230, 1250, 1718, 1899
BccI	CCATC	5 cut(s)	61, 86, 281, 1463, 1505
BceAI	ACGGC	2 cut(s)	1562, 1957
BciT130I	CCWGG	2 cut(s)	297, 1061
BciVI	GTATCC	2 cut(s)	171, 1355
BcnI	CCSGG	2 cut(s)	284, 334
BcoDI	GTCTC	4 cut(s)	101, 151, 1076, 1897
BfaI	CTAG	4 cut(s)	546, 575, 954, 1182
BfmI	CTRYAG	1 cut(s)	1216
BfuI	GTATCC	2 cut(s)	171, 1355
BglII	AGATCT	1 cut(s)	1444
BlnI	CCTAGG	1 cut(s)	1181
BmcAI	AGTACT	1 cut(s)	1792
Bme1390I	CCNGG	4 cut(s)	284, 297, 334, 1061
Bme18I	GGWCC	3 cut(s)	212, 913, 1675
BmeT110I	CYCGRG	1 cut(s)	908
BmgBI	CACGTC	1 cut(s)	1272
BmgT120I	GGNCC	5 cut(s)	62, 212, 820, 913, 1675
BmiI	GGNNCC	6 cut(s)	213, 214, 868, 951, 1677, 1848
BmrFI	CCNGG	4 cut(s)	284, 297, 334, 1061
BmsI	GCATC	3 cut(s)	864, 1357, 1816
BpiI	GAAGAC	2 cut(s)	656, 1025
BplI	GAGNNNNNCTC	2 cut(s)	590, 622
BpmI	CTGGAG	1 cut(s)	711
Bpu10I	CCTNAGC	1 cut(s)	567
Bpu14I	TTCGAA	1 cut(s)	259
BpuEI	CTTGAG	1 cut(s)	1361
BpuMI	CCSGG	2 cut(s)	284, 334
Bsa29I	ATCGAT	2 cut(s)	1625, 1938
BsaAI	YACGTR	1 cut(s)	231
BsaI	GGTCTC	3 cut(s)	101, 1076, 1897
BsaJI	CCNNGG	8 cut(s)	128, 283, 296, 332, 1181, 1231, 1387, 1578
Bsc4I	CCNNNNNNNGG	9 cut(s)	48, 59, 179, 222, 841, 1066, 1182, 1298, 1358
Bse118I	RCCGGY	2 cut(s)	64, 1351
Bse1I	ACTGG	3 cut(s)	694, 1377, 1798
Bse3DI	GCAATG	5 cut(s)	354, 1288, 1362, 1613, 1698
BseBI	CCWGG	2 cut(s)	297, 1061
BseCI	ATCGAT	2 cut(s)	1625, 1938
BseDI	CCNNGG	8 cut(s)	128, 283, 296, 332, 1181, 1231, 1387, 1578
BseGI	GGATG	2 cut(s)	53, 1783
BseLI	CCNNNNNNNGG	9 cut(s)	48, 59, 179, 222, 841, 1066, 1182, 1298, 1358
BseMI	GCAATG	5 cut(s)	354, 1288, 1362, 1613, 1698
BseMII	CTCAG	4 cut(s)	558, 623, 885, 1394
BseNI	ACTGG	3 cut(s)	694, 1377, 1798
BseRI	GAGGAG	1 cut(s)	903
BseXI	GCAGC	9 cut(s)	25, 293, 1100, 1156, 1202, 1230, 1250, 1718, 1899
BseYI	CCCAGC	1 cut(s)	59
BsgI	GTGCAG	1 cut(s)	1155
Bsh1236I	CGCG	1 cut(s)	189
BshFI	GGCC	8 cut(s)	34, 52, 64, 356, 821, 931, 1242, 1972
BshNI	GGYRCC	1 cut(s)	949
BshVI	ATCGAT	2 cut(s)	1625, 1938
BsiHKCI	CYCGRG	1 cut(s)	908
BsiSI	CCGG	5 cut(s)	65, 110, 283, 334, 1352
BslFI	GGGAC	6 cut(s)	198, 585, 697, 1155, 1298, 1661
BslI	CCNNNNNNNGG	9 cut(s)	48, 59, 179, 222, 841, 1066, 1182, 1298, 1358
BsmAI	GTCTC	4 cut(s)	101, 151, 1076, 1897
BsmFI	GGGAC	6 cut(s)	198, 585, 697, 1155, 1298, 1661
BsnI	GGCC	8 cut(s)	34, 52, 64, 356, 821, 931, 1242, 1972
Bso31I	GGTCTC	3 cut(s)	101, 1076, 1897
BsoBI	CYCGRG	1 cut(s)	908
Bsp119I	TTCGAA	1 cut(s)	259
Bsp1407I	TGTACA	1 cut(s)	782
Bsp143I	GATC	8 cut(s)	301, 320, 866, 964, 1296, 1444, 1514, 1873
BspACI	CCGC	3 cut(s)	57, 184, 309
BspANI	GGCC	8 cut(s)	34, 52, 64, 356, 821, 931, 1242, 1972
BspCNI	CTCAG	4 cut(s)	559, 622, 884, 1395
BspDI	ATCGAT	2 cut(s)	1625, 1938
BspFNI	CGCG	1 cut(s)	189
BspLI	GGNNCC	6 cut(s)	213, 214, 868, 951, 1677, 1848
BspMAI	CTGCAG	1 cut(s)	1220
BspPI	GGATC	6 cut(s)	309, 328, 861, 874, 1522, 1881
BspT104I	TTCGAA	1 cut(s)	259
BspT107I	GGYRCC	1 cut(s)	949
BspTNI	GGTCTC	3 cut(s)	101, 1076, 1897
BsrDI	GCAATG	5 cut(s)	354, 1288, 1362, 1613, 1698
BsrFI	RCCGGY	2 cut(s)	64, 1351
BsrGI	TGTACA	1 cut(s)	782
BsrI	ACTGG	3 cut(s)	694, 1377, 1798
BssAI	RCCGGY	2 cut(s)	64, 1351
BssECI	CCNNGG	8 cut(s)	128, 283, 296, 332, 1181, 1231, 1387, 1578
BssMI	GATC	8 cut(s)	301, 320, 866, 964, 1296, 1444, 1514, 1873
BssNAI	GTATAC	1 cut(s)	675
BssT1I	CCWWGG	3 cut(s)	128, 1181, 1231
Bst1107I	GTATAC	1 cut(s)	675
Bst2UI	CCWGG	2 cut(s)	297, 1061
Bst4CI	ACNGT	4 cut(s)	1306, 1685, 1934, 1952
Bst6I	CTCTTC	1 cut(s)	1895
BstAUI	TGTACA	1 cut(s)	782
BstBAI	YACGTR	1 cut(s)	231
BstBI	TTCGAA	1 cut(s)	259
BstC8I	GCNNGC	3 cut(s)	36, 1353, 1506
BstDEI	CTNAG	6 cut(s)	153, 567, 609, 871, 926, 1403
BstDSI	CCRYGG	2 cut(s)	1387, 1578
BstEII	GGTNACC	2 cut(s)	1207, 1427
BstENI	CCTNNNNNAGG	2 cut(s)	1064, 1180
BstF5I	GGATG	2 cut(s)	53, 1783
BstFNI	CGCG	1 cut(s)	189
BstHHI	GCGC	1 cut(s)	189
BstKTI	GATC	8 cut(s)	304, 323, 869, 967, 1299, 1447, 1517, 1876
BstMAI	GTCTC	4 cut(s)	101, 151, 1076, 1897
BstMBI	GATC	8 cut(s)	301, 320, 866, 964, 1296, 1444, 1514, 1873
BstMWI	GCNNNNNNNGC	4 cut(s)	184, 353, 731, 1260
BstNI	CCWGG	2 cut(s)	297, 1061
BstNSI	RCATGY	1 cut(s)	1190
BstPI	GGTNACC	2 cut(s)	1207, 1427
BstSCI	CCNGG	4 cut(s)	282, 295, 332, 1059
BstSFI	CTRYAG	1 cut(s)	1216
BstSNI	TACGTA	1 cut(s)	231
BstUI	CGCG	1 cut(s)	189
BstV1I	GCAGC	9 cut(s)	25, 293, 1100, 1156, 1202, 1230, 1250, 1718, 1899
BstV2I	GAAGAC	2 cut(s)	656, 1025
BstX2I	RGATCY	3 cut(s)	866, 1444, 1873
BstXI	CCANNNNNNTGG	1 cut(s)	1238
BstYI	RGATCY	3 cut(s)	866, 1444, 1873
BstZ17I	GTATAC	1 cut(s)	675
Bsu15I	ATCGAT	2 cut(s)	1625, 1938
BsuI	GTATCC	2 cut(s)	171, 1355
BsuRI	GGCC	8 cut(s)	34, 52, 64, 356, 821, 931, 1242, 1972
BsuTUI	ATCGAT	2 cut(s)	1625, 1938
BtgI	CCRYGG	2 cut(s)	1387, 1578
BtrI	CACGTC	1 cut(s)	1272
BtsCI	GGATG	2 cut(s)	53, 1783
BtsIMutI	CAGTG	3 cut(s)	1353, 1384, 1957
Cac8I	GCNNGC	3 cut(s)	36, 1353, 1506
CfoI	GCGC	1 cut(s)	189
Cfr10I	RCCGGY	2 cut(s)	64, 1351
Cfr13I	GGNCC	5 cut(s)	62, 212, 820, 913, 1675
ClaI	ATCGAT	2 cut(s)	1625, 1938
Csp6I	GTAC	6 cut(s)	232, 783, 950, 1382, 1768, 1791
CviAII	CATG	5 cut(s)	100, 434, 489, 1187, 1864
CviQI	GTAC	6 cut(s)	232, 783, 950, 1382, 1768, 1791
DdeI	CTNAG	6 cut(s)	153, 567, 609, 871, 926, 1403
DpnI	GATC	8 cut(s)	303, 322, 868, 966, 1298, 1446, 1516, 1875
DpnII	GATC	8 cut(s)	301, 320, 866, 964, 1296, 1444, 1514, 1873
DraI	TTTAAA	1 cut(s)	517
DrdI	GACNNNNNNGTC	2 cut(s)	1109, 1149
DseDI	GACNNNNNNGTC	2 cut(s)	1109, 1149
Eam1104I	CTCTTC	1 cut(s)	1895
EarI	CTCTTC	1 cut(s)	1895
Eco105I	TACGTA	1 cut(s)	231
Eco130I	CCWWGG	3 cut(s)	128, 1181, 1231
Eco147I	AGGCCT	2 cut(s)	356, 931
Eco31I	GGTCTC	3 cut(s)	101, 1076, 1897
Eco32I	GATATC	1 cut(s)	895
Eco47I	GGWCC	3 cut(s)	212, 913, 1675
Eco57I	CTGAAG	3 cut(s)	459, 902, 1919
Eco88I	CYCGRG	1 cut(s)	908
Eco91I	GGTNACC	2 cut(s)	1207, 1427
EcoNI	CCTNNNNNAGG	2 cut(s)	1064, 1180
EcoO109I	RGGNCCY	2 cut(s)	212, 913
EcoO65I	GGTNACC	2 cut(s)	1207, 1427
EcoRI	GAATTC	1 cut(s)	602
EcoRII	CCWGG	2 cut(s)	295, 1059
EcoRV	GATATC	1 cut(s)	895
EcoT14I	CCWWGG	3 cut(s)	128, 1181, 1231
ErhI	CCWWGG	3 cut(s)	128, 1181, 1231
FaeI	CATG	5 cut(s)	103, 437, 492, 1190, 1867
FalI	AAGNNNNNCTT	2 cut(s)	1323, 1355
FaqI	GGGAC	6 cut(s)	198, 585, 697, 1155, 1298, 1661
FatI	CATG	5 cut(s)	99, 433, 488, 1186, 1863
FauNDI	CATATG	1 cut(s)	379
FblI	GTMKAC	1 cut(s)	674
FokI	GGATG	2 cut(s)	40, 1790
FspBI	CTAG	4 cut(s)	546, 575, 954, 1182
GlaI	GCGC	1 cut(s)	188
GsaI	CCCAGC	1 cut(s)	63
GsuI	CTGGAG	1 cut(s)	711
HaeIII	GGCC	8 cut(s)	34, 52, 64, 356, 821, 931, 1242, 1972
HapII	CCGG	5 cut(s)	65, 110, 283, 334, 1352
HhaI	GCGC	1 cut(s)	189
Hin1II	CATG	5 cut(s)	103, 437, 492, 1190, 1867
Hin6I	GCGC	1 cut(s)	187
HinP1I	GCGC	1 cut(s)	187
HincII	GTYRAC	1 cut(s)	988
HindII	GTYRAC	1 cut(s)	988
HinfI	GANTC	7 cut(s)	26, 122, 248, 794, 1343, 1652, 1852
HpaI	GTTAAC	1 cut(s)	988
HpaII	CCGG	5 cut(s)	65, 110, 283, 334, 1352
HphI	GGTGA	3 cut(s)	1201, 1499, 1577
Hpy166II	GTNNAC	6 cut(s)	632, 675, 783, 988, 1382, 1840
Hpy188I	TCNGA	6 cut(s)	75, 278, 642, 899, 1301, 1800
Hpy188III	TCNNGA	2 cut(s)	461, 920
Hpy8I	GTNNAC	6 cut(s)	632, 675, 783, 988, 1382, 1840
Hpy99I	CGWCG	1 cut(s)	47
HpyAV	CCTTC	6 cut(s)	10, 486, 503, 926, 1750, 1949
HpyCH4III	ACNGT	4 cut(s)	1306, 1685, 1934, 1952
HpyCH4IV	ACGT	2 cut(s)	230, 1271
HpyF10VI	GCNNNNNNNGC	4 cut(s)	184, 353, 731, 1260
HpyF3I	CTNAG	6 cut(s)	153, 567, 609, 871, 926, 1403
HpySE526I	ACGT	2 cut(s)	230, 1271
Hsp92II	CATG	5 cut(s)	103, 437, 492, 1190, 1867
HspAI	GCGC	1 cut(s)	187
KflI	GGGWCCC	1 cut(s)	212
KpnI	GGTACC	1 cut(s)	953
KroI	GCCGGC	1 cut(s)	1351
KroNI	GCCGGC	1 cut(s)	1353
KspAI	GTTAAC	1 cut(s)	988
Kzo9I	GATC	8 cut(s)	301, 320, 866, 964, 1296, 1444, 1514, 1873
LmnI	GCTCC	4 cut(s)	75, 751, 1696, 1964
Lsp1109I	GCAGC	9 cut(s)	25, 293, 1100, 1156, 1202, 1230, 1250, 1718, 1899
LweI	GCATC	3 cut(s)	864, 1357, 1816
MaeI	CTAG	4 cut(s)	546, 575, 954, 1182
MaeII	ACGT	2 cut(s)	230, 1271
MaeIII	GTNAC	6 cut(s)	193, 716, 1207, 1318, 1427, 1487
MalI	GATC	8 cut(s)	303, 322, 868, 966, 1298, 1446, 1516, 1875
MboI	GATC	8 cut(s)	301, 320, 866, 964, 1296, 1444, 1514, 1873
MboII	GAAGA	9 cut(s)	661, 800, 803, 803, 1027, 1030, 1454, 1802, 1912
MfeI	CAATTG	1 cut(s)	522
MflI	RGATCY	3 cut(s)	866, 1444, 1873
MluCI	AATT	9 cut(s)	261, 499, 511, 522, 602, 708, 726, 1196, 1395
MlyI	GAGTC	2 cut(s)	1352, 1661
MmeI	TCCRAC	2 cut(s)	99, 1910
MroNI	GCCGGC	1 cut(s)	1351
MroXI	GAANNNNTTC	2 cut(s)	265, 1646
MseI	TTAA	7 cut(s)	140, 291, 516, 987, 1785, 1829, 1917
MslI	CAYNNNNRTG	3 cut(s)	668, 828, 1485
MspA1I	CMGCKG	4 cut(s)	38, 59, 1215, 1912
MspI	CCGG	5 cut(s)	65, 110, 283, 334, 1352
MspR9I	CCNGG	4 cut(s)	284, 297, 334, 1061
MunI	CAATTG	1 cut(s)	522
MvaI	CCWGG	2 cut(s)	297, 1061
MvnI	CGCG	1 cut(s)	189
MwoI	GCNNNNNNNGC	4 cut(s)	184, 353, 731, 1260
NaeI	GCCGGC	1 cut(s)	1353
NciI	CCSGG	2 cut(s)	284, 334
NdeI	CATATG	1 cut(s)	379
NdeII	GATC	8 cut(s)	301, 320, 866, 964, 1296, 1444, 1514, 1873
NgoMIV	GCCGGC	1 cut(s)	1351
NlaIII	CATG	5 cut(s)	103, 437, 492, 1190, 1867
NlaIV	GGNNCC	6 cut(s)	213, 214, 868, 951, 1677, 1848
NmuCI	GTSAC	4 cut(s)	193, 1207, 1318, 1487
NspI	RCATGY	1 cut(s)	1190
NspV	TTCGAA	1 cut(s)	259
PaeR7I	CTCGAG	1 cut(s)	908
PceI	AGGCCT	2 cut(s)	356, 931
PdiI	GCCGGC	1 cut(s)	1353
PdmI	GAANNNNTTC	2 cut(s)	265, 1646
PfeI	GAWTC	5 cut(s)	26, 122, 248, 794, 1852
PflFI	GACNNNGTC	1 cut(s)	1149
PflMI	CCANNNNNTGG	3 cut(s)	59, 179, 841
PleI	GAGTC	2 cut(s)	1351, 1660
PpsI	GAGTC	2 cut(s)	1351, 1660
Ppu21I	YACGTR	1 cut(s)	231
PpuMI	RGGWCCY	2 cut(s)	212, 913
PsiI	TTATAA	1 cut(s)	1041
Psp5II	RGGWCCY	2 cut(s)	212, 913
Psp6I	CCWGG	2 cut(s)	295, 1059
PspEI	GGTNACC	2 cut(s)	1207, 1427
PspFI	CCCAGC	1 cut(s)	59
PspGI	CCWGG	2 cut(s)	295, 1059
PspN4I	GGNNCC	6 cut(s)	213, 214, 868, 951, 1677, 1848
PspPI	GGNCC	5 cut(s)	62, 212, 820, 913, 1675
PspPPI	RGGWCCY	2 cut(s)	212, 913
PspXI	VCTCGAGB	1 cut(s)	908
PstI	CTGCAG	1 cut(s)	1220
PsuI	RGATCY	3 cut(s)	866, 1444, 1873
PsyI	GACNNNGTC	1 cut(s)	1149
PvuII	CAGCTG	3 cut(s)	38, 1215, 1912
RsaI	GTAC	6 cut(s)	233, 784, 951, 1383, 1769, 1792
RsaNI	GTAC	6 cut(s)	232, 783, 950, 1382, 1768, 1791
RseI	CAYNNNNRTG	3 cut(s)	668, 828, 1485
SaqAI	TTAA	7 cut(s)	140, 291, 516, 987, 1785, 1829, 1917
Sau3AI	GATC	8 cut(s)	301, 320, 866, 964, 1296, 1444, 1514, 1873
Sau96I	GGNCC	5 cut(s)	62, 212, 820, 913, 1675
ScaI	AGTACT	1 cut(s)	1792
SchI	GAGTC	2 cut(s)	1352, 1661
ScrFI	CCNGG	4 cut(s)	284, 297, 334, 1061
SfaNI	GCATC	3 cut(s)	864, 1357, 1816
SfcI	CTRYAG	1 cut(s)	1216
Sfr274I	CTCGAG	1 cut(s)	908
SfuI	TTCGAA	1 cut(s)	259
SinI	GGWCC	3 cut(s)	212, 913, 1675
SlaI	CTCGAG	1 cut(s)	908
SmiMI	CAYNNNNRTG	3 cut(s)	668, 828, 1485
SmlI	CTYRAG	2 cut(s)	908, 1340
SmoI	CTYRAG	2 cut(s)	908, 1340
SnaBI	TACGTA	1 cut(s)	231
Sse9I	AATT	9 cut(s)	261, 499, 511, 522, 602, 708, 726, 1196, 1395
SseBI	AGGCCT	2 cut(s)	356, 931
SsiI	CCGC	3 cut(s)	57, 184, 309
SspI	AATATT	1 cut(s)	1922
SspMI	CTAG	4 cut(s)	546, 575, 954, 1182
StuI	AGGCCT	2 cut(s)	356, 931
StyD4I	CCNGG	4 cut(s)	282, 295, 332, 1059
StyI	CCWWGG	3 cut(s)	128, 1181, 1231
TaaI	ACNGT	4 cut(s)	1306, 1685, 1934, 1952
TaiI	ACGT	2 cut(s)	233, 1274
TaqI	TCGA	8 cut(s)	29, 259, 909, 1625, 1650, 1754, 1813, 1938
TasI	AATT	9 cut(s)	261, 499, 511, 522, 602, 708, 726, 1196, 1395
TatI	WGTACW	3 cut(s)	782, 1767, 1790
TauI	GCSGC	1 cut(s)	187
TfiI	GAWTC	5 cut(s)	26, 122, 248, 794, 1852
Tru1I	TTAA	7 cut(s)	140, 291, 516, 987, 1785, 1829, 1917
Tru9I	TTAA	7 cut(s)	140, 291, 516, 987, 1785, 1829, 1917
TscAI	CASTG	3 cut(s)	1353, 1384, 1957
TseFI	GTSAC	4 cut(s)	193, 1207, 1318, 1487
TseI	GCWGC	9 cut(s)	38, 306, 1088, 1169, 1215, 1218, 1263, 1706, 1912
Tsp45I	GTSAC	4 cut(s)	193, 1207, 1318, 1487
TspDTI	ATGAA	5 cut(s)	412, 786, 987, 1469, 1535
TspGWI	ACGGA	1 cut(s)	661
TspRI	CASTG	3 cut(s)	1353, 1384, 1957
Tth111I	GACNNNGTC	1 cut(s)	1149
Van91I	CCANNNNNTGG	3 cut(s)	59, 179, 841
VpaK11BI	GGWCC	3 cut(s)	212, 913, 1675
XagI	CCTNNNNNAGG	2 cut(s)	1064, 1180
XapI	RAATTY	3 cut(s)	602, 1196, 1395
XceI	RCATGY	1 cut(s)	1190
XhoI	CTCGAG	1 cut(s)	908
XmaJI	CCTAGG	1 cut(s)	1181
XmiI	GTMKAC	1 cut(s)	674
XmnI	GAANNNNTTC	2 cut(s)	265, 1646
XspI	CTAG	4 cut(s)	546, 575, 954, 1182
ZrmI	AGTACT	1 cut(s)	1792

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.