Long chain acyl-CoA synthetase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 67 |
| AcuI | CTGAAG | 1 cut(s) | 60 |
| AgsI | TTSAA | 3 cut(s) | 48, 62, 119 |
| AluBI | AGCT | 2 cut(s) | 53, 133 |
| AluI | AGCT | 2 cut(s) | 53, 133 |
| AlwI | GGATC | 1 cut(s) | 67 |
| BfaI | CTAG | 1 cut(s) | 148 |
| Bsp143I | GATC | 1 cut(s) | 72 |
| BspPI | GGATC | 1 cut(s) | 67 |
| BssMI | GATC | 1 cut(s) | 72 |
| BstKTI | GATC | 1 cut(s) | 75 |
| BstMBI | GATC | 1 cut(s) | 72 |
| BstX2I | RGATCY | 1 cut(s) | 72 |
| BstYI | RGATCY | 1 cut(s) | 72 |
| CviAII | CATG | 2 cut(s) | 35, 90 |
| CviJI | RGCY | 2 cut(s) | 53, 133 |
| CviKI_1 | RGCY | 2 cut(s) | 53, 133 |
| DpnI | GATC | 1 cut(s) | 74 |
| DpnII | GATC | 1 cut(s) | 72 |
| Eco57I | CTGAAG | 1 cut(s) | 60 |
| FaeI | CATG | 2 cut(s) | 38, 93 |
| FaiI | YATR | 3 cut(s) | 36, 86, 91 |
| FatI | CATG | 2 cut(s) | 34, 89 |
| FspBI | CTAG | 1 cut(s) | 148 |
| FspEI | CC | 5 cut(s) | 47, 89, 90, 107, 111 |
| Hin1II | CATG | 2 cut(s) | 38, 93 |
| Hpy188III | TCNNGA | 1 cut(s) | 62 |
| HpyAV | CCTTC | 1 cut(s) | 104 |
| HpyCH4V | TGCA | 1 cut(s) | 14 |
| Hsp92II | CATG | 2 cut(s) | 38, 93 |
| Kzo9I | GATC | 1 cut(s) | 72 |
| LpnPI | CCDG | 1 cut(s) | 90 |
| MaeI | CTAG | 1 cut(s) | 148 |
| MalI | GATC | 1 cut(s) | 74 |
| MboI | GATC | 1 cut(s) | 72 |
| MfeI | CAATTG | 1 cut(s) | 124 |
| MflI | RGATCY | 1 cut(s) | 72 |
| MluCI | AATT | 2 cut(s) | 101, 124 |
| MunI | CAATTG | 1 cut(s) | 124 |
| NdeII | GATC | 1 cut(s) | 72 |
| NlaIII | CATG | 2 cut(s) | 38, 93 |
| PsuI | RGATCY | 1 cut(s) | 72 |
| Sau3AI | GATC | 1 cut(s) | 72 |
| SetI | ASST | 2 cut(s) | 55, 135 |
| SgeI | CNNG | 5 cut(s) | 47, 74, 82, 89, 102 |
| Sse9I | AATT | 2 cut(s) | 101, 124 |
| SspMI | CTAG | 1 cut(s) | 148 |
| TasI | AATT | 2 cut(s) | 101, 124 |
| TspDTI | ATGAA | 1 cut(s) | 13 |
| XspI | CTAG | 1 cut(s) | 148 |