Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0000749

Physical Location & Seq

Genomic Coordinates Reverse (-)

4528468 .. 4530264

Gene Structure 1797 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1064100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1581 bp

ATGGATCGGCATCATGGTTCTGAAGATTACGAGGAGGAAGAAGGAGCTCATAGTCAACCCCGCCAGAGAAATATGACCAAATCGAAACCTGCTGGAGGAGGGTACGTAGACGAAGATGACCAAAGCCAACATCGGCAGATAAAACCAGCTTACGGCGGTTCTCGCGAAGAGTATGGCGACGAAGAAGATCGAGACCGACATGGTGAGAGAAAATCCAGCAAACCGAAATCATCAGCAGCTTACAGTTCGAGGGAAGATGAAGATGAATATGACGAGAGAAAACCCAACAAGCCGAAAGCCTCTGCGAAAGCAGCTTACGGTTCGACGGAAGATGAAGATGAGTACGGCGAGAGAAACCACAATAAGCCGAAAGCCGCTGCGAAAGCGGCTTACGGTTCGAGGGAAGATGAAGATGAATACGGCGAGACAAAACCCAACAAGCCGAAAGCCGCTCCGAAAACTGCTTACGGTTCAACGGGAGATGAAGATGAATACGGCGAGACAAAACCCAACAAACCGAAAGCCGCTCCGAAAACTGCTTACGGTTCAACGGAAGATGAAGATGAATACGATGAAGATGAATACGGCGAGACAAAACCCAACAAGCCGAAAGCCGCTGCGAAAACGGCTTACGGTTCAACGGAAGATGAAGATGAATACGGCGAGCGAAAATCCAACAAGCCGAAAACCGTTGCGAAAGCGGCGGCGACTTACGGTTCGACGGAAGATGAAGATGAATACGGCGAGAGGAAACCCAACAAGCCGAAAGCTGCTGTGAAAGCGGCTTACGGTTCAAGTGAAGAGTATGGGGGAGAAGATGATCAGGATGAATATGGCCAAAGAAAACCTGGCAAACCAAAGGCCGCTGCGAAACAAGCTTATGGTTCTGAGGGTTATGGAGATGAAGATGATCAGGAACATCGCGAAAAAAAACCTAGCTCGGGTTCTGCCGGGAAAGCAGCCAAAGGCGCGAAACCTGAAAAAGATGGGGATGTGGAGGATGATCAATACCTCGAGAAAAAAGCTGTTGCTGCTAAACCATCAAAGTCAACTTATGGGGATGAAAAGGATGAGCAGAGTGAACATCGTGAGCGGAAAGTGGGCACTGACGGGAAACATGTTGGTGGTGTGGGGGCGGAGAAAGCGAAAGCGGGAGGAGCGAAGGAGAAAGGTGAAAAGCCATCAATGGGGGAGGTGATGTCGAGTGCGAAGATTGTGGCGGGCGCTAATAAAGGGGACAAGTTCGACAAAGCTGAGGTTTGCAAGGCTGCCAGTACCATTCTAGGTGCTGCCTCTGCGTACGGCGGTTTCGACGACAAAGAAGGCATCGGGAGTTACCTCAGCAAGGCAGAGGATATGCTTCGCAAGTATGGTGGCGGCGGCCAAGAAGGTGAGGGAGGTGGCCCCAAAACTTCTGTTTCTCATTCTTCAGCTTCTGAGGAGAAGAAGAAGAAAAGTAATGATTCAAATGAGAGCGGTGGGGGCCCGAAGAAGCCCACTAAAAAGGATGACGGTGAAGAGGATGGTGGTGGTGGAGTTGGGGGCTATCTGAAGATGGCTCAGGGTTTCTGGAAGGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002831 GO:0002833 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0009266 GO:0009408 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009987 GO:0010033 GO:0023052 GO:0031347 GO:0031349 GO:0032101 GO:0032103 GO:0032870 GO:0033993 GO:0042221 GO:0043207 GO:0043900 GO:0043902 GO:0048518 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0080134 GO:0097305 GO:0097306 GO:1900424 GO:1900426 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

527

Amino Acids

56.45

Weight (kDa)

5.17

Isoelectric Point (pI)

36.73

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000749)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13930 AT1G13930 AT1G13930 AT2G03440
fragaria_vesca	FvH4_4g30460
malus_domestica	MD13G1065600.v1.1 MD13G1065700.v1.1 MD16G1064100.v1.1 MD16G1064200.v1.1
prunus_persica	Prupe.1G282500_v2.0.a1
pyrus_communis	pycom13g05780 pycom13g05790
rosa_chinensis	RchiOBHm_Chr1g0320671 RchiOBHm_Chr2g0084691 RchiOBHm_Chr4g0391511 RchiOBHm_Chr4g0397231 RchiOBHm_Chr5g0038511 RchiOBHm_Chr7g0218611 RchiOBHm_Chr7g0243091
rosa_laevigata	RLG00000001214 RLG00000003134 RLG00000005591 RLG00000006330 RLG00000008137 RLG00000020620 RLG00000024858 RLG00000027359
rosa_multiflora	Rmu_co8437595.1_g000001 Rmu_sc0001781.1_g000012 Rmu_sc0001796.1_g000001 Rmu_sc0002245.1_g000048 Rmu_sc0002312.1_g000067 Rmu_sc0006553.1_g000012 Rmu_sc0007391.1_g000013 Rmu_ssc0000436.1_g000004
rosa_roxburghii	Rroxscaffold_1G00072700 Rroxscaffold_3G00245360 Rroxscaffold_3G00267980 Rroxscaffold_4G00319730 Rroxscaffold_5G00374780 Rroxscaffold_7G00197900
rosa_rugosa	Rorug01G0161500.1 Rorug04G0313200 Rorug04G0313200 Rorug07G0153800
rosa_samantha	Rh1BG233900 Rh1DG068600 Rh1DG091400 Rh1DG261300 Rh2AG113800 Rh2AG464700 Rh4AG364000 Rh4BG288300 Rh4BG375300 Rh4CG180100 Rh4CG389400 Rh4DG224500 Rh4DG369700 Rh5AG078000 Rh5CG365500 Rh5DG489000 Rh5DG546500 Rh6BG092900 Rh7BG230100 Rh7BG332300 Rh7DG399200
rosa_wichuraiana	Rw0G007230 Rw0G016780 Rw2G045830 Rw4G006320 Rw5G027470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	97
AccBSI	CCGCTC	4 cut(s)	452, 527, 1093, 1476
AccI	GTMKAC	1 cut(s)	108
AccII	CGCG	3 cut(s)	165, 924, 971
AclWI	GGATC	1 cut(s)	12
AcoI	YGGCCR	2 cut(s)	835, 1381
AcuI	CTGAAG	3 cut(s)	42, 1413, 1571
AfaI	GTAC	4 cut(s)	104, 344, 1276, 1301
AfiI	CCNNNNNNNGG	5 cut(s)	95, 152, 941, 1187, 1345
AflIII	ACRYGT	1 cut(s)	1117
AgsI	TTSAA	5 cut(s)	474, 549, 639, 795, 1467
AjnI	CCWGG	1 cut(s)	847
Alw21I	GWGCWC	1 cut(s)	49
Alw26I	GTCTC	4 cut(s)	186, 419, 494, 584
AlwI	GGATC	1 cut(s)	12
AlwNI	CAGNNNCTG	1 cut(s)	1436
Ama87I	CYCGRG	2 cut(s)	940, 1013
AoxI	GGCC	5 cut(s)	835, 861, 1381, 1402, 1483
ApaI	GGGCCC	1 cut(s)	1487
ArsI	GACNNNNNNTTYG	2 cut(s)	700, 732
AspLEI	GCGC	2 cut(s)	971, 1226
AspS9I	GGNCC	3 cut(s)	1403, 1483, 1484
AsuC2I	CCSGG	1 cut(s)	952
AsuHPI	GGTGA	5 cut(s)	215, 1184, 1207, 1403, 1526
AvaI	CYCGRG	2 cut(s)	940, 1013
BaeGI	GKGCMC	2 cut(s)	1106, 1487
BalI	TGGCCA	1 cut(s)	837
BanII	GRGCYC	2 cut(s)	49, 1487
Bbv12I	GWGCWC	1 cut(s)	49
BbvCI	CCTCAGC	2 cut(s)	1254, 1340
BccI	CCATC	5 cut(s)	980, 1048, 1189, 1517, 1549
BceAI	ACGGC	9 cut(s)	169, 361, 436, 511, 601, 642, 676, 757, 1318
BciT130I	CCWGG	1 cut(s)	849
BclI	TGATCA	3 cut(s)	820, 910, 1003
BcnI	CCSGG	1 cut(s)	952
BcoDI	GTCTC	4 cut(s)	186, 419, 494, 584
BfaI	CTAG	2 cut(s)	936, 1283
BfoI	RGCGCY	1 cut(s)	1227
BfuAI	ACCTGC	1 cut(s)	97
Bme1390I	CCNGG	2 cut(s)	849, 952
BmeT110I	CYCGRG	2 cut(s)	940, 1013
BmgT120I	GGNCC	3 cut(s)	1403, 1483, 1484
BmiI	GGNNCC	3 cut(s)	1405, 1484, 1485
BmrFI	CCNGG	2 cut(s)	849, 952
BmsI	GCATC	2 cut(s)	19, 1335
BpmI	CTGGAG	1 cut(s)	114
Bpu10I	CCTNAGC	3 cut(s)	1254, 1340, 1560
BpuMI	CCSGG	1 cut(s)	952
BsaAI	YACGTR	1 cut(s)	106
BsaBI	GATNNNNATC	1 cut(s)	9
BsaI	GGTCTC	1 cut(s)	186
BsaXI	ACNNNNNCTCC	4 cut(s)	90, 120, 1184, 1214
Bsc4I	CCNNNNNNNGG	5 cut(s)	95, 152, 941, 1187, 1345
Bse1I	ACTGG	1 cut(s)	1272
Bse8I	GATNNNNATC	1 cut(s)	9
BseBI	CCWGG	1 cut(s)	849
BseGI	GGATG	7 cut(s)	832, 997, 1006, 1066, 1075, 1513, 1528
BseJI	GATNNNNATC	1 cut(s)	9
BseLI	CCNNNNNNNGG	5 cut(s)	95, 152, 941, 1187, 1345
BseMII	CTCAG	5 cut(s)	879, 1245, 1354, 1428, 1574
BseNI	ACTGG	1 cut(s)	1272
BseRI	GAGGAG	4 cut(s)	47, 111, 1170, 1454
BseSI	GKGCMC	2 cut(s)	1106, 1487
Bsh1236I	CGCG	3 cut(s)	165, 924, 971
BshFI	GGCC	5 cut(s)	837, 863, 1383, 1404, 1485
BsiHKAI	GWGCWC	1 cut(s)	49
BsiHKCI	CYCGRG	2 cut(s)	940, 1013
BsiSI	CCGG	1 cut(s)	951
BsiWI	CGTACG	1 cut(s)	1299
BslFI	GGGAC	1 cut(s)	1250
BslI	CCNNNNNNNGG	5 cut(s)	95, 152, 941, 1187, 1345
BsmAI	GTCTC	4 cut(s)	186, 419, 494, 584
BsmFI	GGGAC	1 cut(s)	1250
BsnI	GGCC	5 cut(s)	837, 863, 1383, 1404, 1485
Bso31I	GGTCTC	1 cut(s)	186
BsoBI	CYCGRG	2 cut(s)	940, 1013
Bsp120I	GGGCCC	1 cut(s)	1483
Bsp1286I	GDGCHC	3 cut(s)	49, 1106, 1487
Bsp143I	GATC	5 cut(s)	4, 187, 820, 910, 1003
Bsp68I	TCGCGA	2 cut(s)	165, 924
BspANI	GGCC	5 cut(s)	837, 863, 1383, 1404, 1485
BspCNI	CTCAG	5 cut(s)	880, 1246, 1353, 1429, 1573
BspFNI	CGCG	3 cut(s)	165, 924, 971
BspLI	GGNNCC	3 cut(s)	1405, 1484, 1485
BspMI	ACCTGC	1 cut(s)	97
BspPI	GGATC	1 cut(s)	12
BspTNI	GGTCTC	1 cut(s)	186
BsrBI	CCGCTC	4 cut(s)	452, 527, 1093, 1476
BsrI	ACTGG	1 cut(s)	1272
BssMI	GATC	5 cut(s)	4, 187, 820, 910, 1003
Bst2UI	CCWGG	1 cut(s)	849
Bst6I	CTCTTC	3 cut(s)	162, 795, 1512
BstBAI	YACGTR	1 cut(s)	106
BstC8I	GCNNGC	2 cut(s)	665, 1222
BstDEI	CTNAG	5 cut(s)	888, 1254, 1340, 1437, 1560
BstENI	CCTNNNNNAGG	2 cut(s)	93, 1343
BstF5I	GGATG	7 cut(s)	832, 997, 1006, 1066, 1075, 1513, 1528
BstFNI	CGCG	3 cut(s)	165, 924, 971
BstH2I	RGCGCY	1 cut(s)	1227
BstHHI	GCGC	2 cut(s)	971, 1226
BstKTI	GATC	5 cut(s)	7, 190, 823, 913, 1006
BstMAI	GTCTC	4 cut(s)	186, 419, 494, 584
BstMBI	GATC	5 cut(s)	4, 187, 820, 910, 1003
BstNI	CCWGG	1 cut(s)	849
BstNSI	RCATGY	1 cut(s)	1121
BstSCI	CCNGG	2 cut(s)	847, 950
BstSLI	GKGCMC	2 cut(s)	1106, 1487
BstSNI	TACGTA	1 cut(s)	106
BstUI	CGCG	3 cut(s)	165, 924, 971
BsuRI	GGCC	5 cut(s)	837, 863, 1383, 1404, 1485
BtgZI	GCGATG	1 cut(s)	905
BtsCI	GGATG	7 cut(s)	832, 997, 1006, 1066, 1075, 1513, 1528
BtsIMutI	CAGTG	1 cut(s)	1104
BtuMI	TCGCGA	2 cut(s)	165, 924
BveI	ACCTGC	1 cut(s)	97
Cac8I	GCNNGC	2 cut(s)	665, 1222
CaiI	CAGNNNCTG	1 cut(s)	1436
CfoI	GCGC	2 cut(s)	971, 1226
Cfr13I	GGNCC	3 cut(s)	1403, 1483, 1484
Csp6I	GTAC	4 cut(s)	103, 343, 1275, 1300
CspCI	CAANNNNNGTGG	2 cut(s)	1354, 1389
CviAII	CATG	3 cut(s)	14, 200, 1118
CviQI	GTAC	4 cut(s)	103, 343, 1275, 1300
DdeI	CTNAG	5 cut(s)	888, 1254, 1340, 1437, 1560
DpnI	GATC	5 cut(s)	6, 189, 822, 912, 1005
DpnII	GATC	5 cut(s)	4, 187, 820, 910, 1003
EaeI	YGGCCR	2 cut(s)	835, 1381
Eam1104I	CTCTTC	3 cut(s)	162, 795, 1512
EarI	CTCTTC	3 cut(s)	162, 795, 1512
EciI	GGCGGA	1 cut(s)	1151
Ecl136II	GAGCTC	1 cut(s)	47
Eco105I	TACGTA	1 cut(s)	106
Eco24I	GRGCYC	2 cut(s)	49, 1487
Eco31I	GGTCTC	1 cut(s)	186
Eco53kI	GAGCTC	1 cut(s)	47
Eco57I	CTGAAG	3 cut(s)	42, 1413, 1571
Eco88I	CYCGRG	2 cut(s)	940, 1013
EcoICRI	GAGCTC	1 cut(s)	47
EcoNI	CCTNNNNNAGG	2 cut(s)	93, 1343
EcoO109I	RGGNCCY	1 cut(s)	1483
EcoRII	CCWGG	1 cut(s)	847
EcoT38I	GRGCYC	2 cut(s)	49, 1487
FaeI	CATG	3 cut(s)	17, 203, 1121
FaqI	GGGAC	1 cut(s)	1250
FatI	CATG	3 cut(s)	13, 199, 1117
FauI	CCCGC	3 cut(s)	68, 1144, 1213
FbaI	TGATCA	3 cut(s)	820, 910, 1003
FblI	GTMKAC	1 cut(s)	108
FokI	GGATG	7 cut(s)	839, 1004, 1013, 1073, 1082, 1520, 1535
FriOI	GRGCYC	2 cut(s)	49, 1487
FspBI	CTAG	2 cut(s)	936, 1283
GlaI	GCGC	2 cut(s)	970, 1225
GsuI	CTGGAG	1 cut(s)	114
HaeII	RGCGCY	1 cut(s)	1227
HaeIII	GGCC	5 cut(s)	837, 863, 1383, 1404, 1485
HapII	CCGG	1 cut(s)	951
HhaI	GCGC	2 cut(s)	971, 1226
Hin1II	CATG	3 cut(s)	17, 203, 1121
Hin6I	GCGC	2 cut(s)	969, 1224
HinP1I	GCGC	2 cut(s)	969, 1224
HincII	GTYRAC	2 cut(s)	56, 1050
HindII	GTYRAC	2 cut(s)	56, 1050
HindIII	AAGCTT	1 cut(s)	876
HinfI	GANTC	1 cut(s)	1463
HpaII	CCGG	1 cut(s)	951
HphI	GGTGA	5 cut(s)	215, 1184, 1207, 1403, 1526
Hpy166II	GTNNAC	4 cut(s)	56, 109, 1050, 1082
Hpy188I	TCNGA	6 cut(s)	22, 456, 531, 889, 1438, 1551
Hpy188III	TCNNGA	9 cut(s)	164, 191, 824, 914, 923, 1015, 1088, 1330, 1570
Hpy8I	GTNNAC	4 cut(s)	56, 109, 1050, 1082
Hpy99I	CGWCG	4 cut(s)	182, 328, 724, 1316
HpyAV	CCTTC	5 cut(s)	35, 1156, 1316, 1382, 1567
HpyCH4IV	ACGT	1 cut(s)	105
HpyCH4V	TGCA	1 cut(s)	1263
HpyF3I	CTNAG	5 cut(s)	888, 1254, 1340, 1437, 1560
HpySE526I	ACGT	1 cut(s)	105
Hsp92II	CATG	3 cut(s)	17, 203, 1121
HspAI	GCGC	2 cut(s)	969, 1224
Ksp22I	TGATCA	3 cut(s)	820, 910, 1003
Kzo9I	GATC	5 cut(s)	4, 187, 820, 910, 1003
LmnI	GCTCC	4 cut(s)	44, 457, 532, 1157
LweI	GCATC	2 cut(s)	19, 1335
MaeI	CTAG	2 cut(s)	936, 1283
MaeII	ACGT	1 cut(s)	105
MaeIII	GTNAC	1 cut(s)	1334
MalI	GATC	5 cut(s)	6, 189, 822, 912, 1005
MbiI	CCGCTC	4 cut(s)	452, 527, 1093, 1476
MboI	GATC	5 cut(s)	4, 187, 820, 910, 1003
MhlI	GDGCHC	3 cut(s)	49, 1106, 1487
MlsI	TGGCCA	1 cut(s)	837
MluNI	TGGCCA	1 cut(s)	837
MmeI	TCCRAC	1 cut(s)	699
Mox20I	TGGCCA	1 cut(s)	837
MscI	TGGCCA	1 cut(s)	837
MslI	CAYNNNNRTG	1 cut(s)	1122
Msp20I	TGGCCA	1 cut(s)	837
MspA1I	CMGCKG	3 cut(s)	377, 617, 866
MspI	CCGG	1 cut(s)	951
MspR9I	CCNGG	2 cut(s)	849, 952
MvaI	CCWGG	1 cut(s)	849
MvnI	CGCG	3 cut(s)	165, 924, 971
NciI	CCSGG	1 cut(s)	952
NdeII	GATC	5 cut(s)	4, 187, 820, 910, 1003
NlaIII	CATG	3 cut(s)	17, 203, 1121
NlaIV	GGNNCC	3 cut(s)	1405, 1484, 1485
NruI	TCGCGA	2 cut(s)	165, 924
NspI	RCATGY	1 cut(s)	1121
PaeR7I	CTCGAG	1 cut(s)	1013
PciI	ACATGT	1 cut(s)	1117
PcsI	WCGNNNNNNNCGW	1 cut(s)	1308
PfeI	GAWTC	1 cut(s)	1463
Pfl23II	CGTACG	1 cut(s)	1299
Ppu21I	YACGTR	1 cut(s)	106
PscI	ACATGT	1 cut(s)	1117
Psp124BI	GAGCTC	1 cut(s)	49
Psp6I	CCWGG	1 cut(s)	847
PspGI	CCWGG	1 cut(s)	847
PspLI	CGTACG	1 cut(s)	1299
PspN4I	GGNNCC	3 cut(s)	1405, 1484, 1485
PspOMI	GGGCCC	1 cut(s)	1483
PspPI	GGNCC	3 cut(s)	1403, 1483, 1484
PstNI	CAGNNNCTG	1 cut(s)	1436
RruI	TCGCGA	2 cut(s)	165, 924
RsaI	GTAC	4 cut(s)	104, 344, 1276, 1301
RsaNI	GTAC	4 cut(s)	103, 343, 1275, 1300
RseI	CAYNNNNRTG	1 cut(s)	1122
SacI	GAGCTC	1 cut(s)	49
Sau3AI	GATC	5 cut(s)	4, 187, 820, 910, 1003
Sau96I	GGNCC	3 cut(s)	1403, 1483, 1484
ScrFI	CCNGG	2 cut(s)	849, 952
SduI	GDGCHC	3 cut(s)	49, 1106, 1487
SfaNI	GCATC	2 cut(s)	19, 1335
Sfr274I	CTCGAG	1 cut(s)	1013
SlaI	CTCGAG	1 cut(s)	1013
SmiMI	CAYNNNNRTG	1 cut(s)	1122
SmlI	CTYRAG	1 cut(s)	1013
SmoI	CTYRAG	1 cut(s)	1013
SnaBI	TACGTA	1 cut(s)	106
SspMI	CTAG	2 cut(s)	936, 1283
SstI	GAGCTC	1 cut(s)	49
StyD4I	CCNGG	2 cut(s)	847, 950
TaiI	ACGT	1 cut(s)	108
TaqII	GACCGA	1 cut(s)	210
TfiI	GAWTC	1 cut(s)	1463
TscAI	CASTG	1 cut(s)	1111
TspGWI	ACGGA	4 cut(s)	341, 566, 656, 737
TspRI	CASTG	1 cut(s)	1111
XagI	CCTNNNNNAGG	2 cut(s)	93, 1343
XceI	RCATGY	1 cut(s)	1121
XcmI	CCANNNNNNNNNTGG	1 cut(s)	845
XhoI	CTCGAG	1 cut(s)	1013
XmiI	GTMKAC	1 cut(s)	108
XspI	CTAG	2 cut(s)	936, 1283

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.