Rh1BG233900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000749
Physical Location & Seq
Reverse (-)
35661490 .. 35661774
285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG233900.1

Sequence Viewer

Length: 285 bp
ATGTTTGCCTGGGCCTGCGGCATGGTTCTTGGGCGATGGTCTGACTCAAGGATGGAGGAAGGCGCGGTGGTGGCTGCATCTGGATCTGTGGCGTGGCTGAGGTTTGAGACCGGTCCAGCGTCGGATCTTAGTCGGATTGGGGAGGTGTCTTGCGTCGGTCGGAAGGTGTGGCGGGATCTACGGTTTGCTGCTGCGGATCGATGGATCGACTGTGGTCTTTTGGTGTTTGATTTCAGTTTCTGTCGTAGATCCATGGGTTCTGGATGTTTGGGTCTACTGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

94

Amino Acids

10.4

Weight (kDa)

7.61

Isoelectric Point (pI)

54.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 274
AccII CGCG 1 cut(s) 65
AciI CCGC 4 cut(s) 18, 65, 172, 194
AclWI GGATC 6 cut(s) 91, 132, 183, 204, 212, 243
AgeI ACCGGT 1 cut(s) 110
AjnI CCWGG 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 101
AlwI GGATC 6 cut(s) 91, 132, 183, 204, 212, 243
AlwNI CAGNNNCTG 1 cut(s) 240
AoxI GGCC 1 cut(s) 12
ApeKI GCWGC 3 cut(s) 74, 188, 191
AsiGI ACCGGT 1 cut(s) 110
AspLEI GCGC 1 cut(s) 65
AspS9I GGNCC 2 cut(s) 12, 113
AvaII GGWCC 1 cut(s) 113
BbvCI CCTCAGC 1 cut(s) 98
BbvI GCAGC 3 cut(s) 61, 175, 178
BccI CCATC 3 cut(s) 30, 46, 195
BciT130I CCWGG 1 cut(s) 10
BcoDI GTCTC 1 cut(s) 101
BisI GCNGC 4 cut(s) 19, 75, 189, 192
BlsI GCNGC 4 cut(s) 20, 76, 190, 193
Bme1390I CCNGG 1 cut(s) 10
Bme18I GGWCC 1 cut(s) 113
BmgT120I GGNCC 2 cut(s) 12, 113
BmrFI CCNGG 1 cut(s) 10
BmsI GCATC 1 cut(s) 86
BoxI GACNNNNGTC 1 cut(s) 213
Bpu10I CCTNAGC 1 cut(s) 98
BpuEI CTTGAG 1 cut(s) 31
Bsa29I ATCGAT 1 cut(s) 199
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 2 cut(s) 9, 252
BsaWI WCCGGW 1 cut(s) 110
Bse118I RCCGGY 1 cut(s) 110
BseBI CCWGG 1 cut(s) 10
BseCI ATCGAT 1 cut(s) 199
BseDI CCNNGG 2 cut(s) 9, 252
BseGI GGATG 2 cut(s) 57, 269
BseMII CTCAG 1 cut(s) 89
BseXI GCAGC 3 cut(s) 61, 175, 178
Bsh1236I CGCG 1 cut(s) 65
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 1 cut(s) 14
BshTI ACCGGT 1 cut(s) 110
BshVI ATCGAT 1 cut(s) 199
BsiEI CGRYCG 1 cut(s) 160
BsiSI CCGG 1 cut(s) 111
BsmAI GTCTC 1 cut(s) 101
BsnI GGCC 1 cut(s) 14
Bso31I GGTCTC 1 cut(s) 101
Bsp143I GATC 6 cut(s) 83, 124, 175, 196, 204, 248
Bsp19I CCATGG 1 cut(s) 252
BspACI CCGC 4 cut(s) 18, 65, 172, 194
BspANI GGCC 1 cut(s) 14
BspCNI CTCAG 1 cut(s) 90
BspDI ATCGAT 1 cut(s) 199
BspFNI CGCG 1 cut(s) 65
BspPI GGATC 6 cut(s) 91, 132, 183, 204, 212, 243
BspTNI GGTCTC 1 cut(s) 101
BsrFI RCCGGY 1 cut(s) 110
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 2 cut(s) 9, 252
BssMI GATC 6 cut(s) 83, 124, 175, 196, 204, 248
BssT1I CCWWGG 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 10
Bst4CI ACNGT 3 cut(s) 183, 212, 279
BstC8I GCNNGC 1 cut(s) 16
BstDEI CTNAG 2 cut(s) 98, 128
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 2 cut(s) 57, 269
BstFNI CGCG 1 cut(s) 65
BstHHI GCGC 1 cut(s) 65
BstKTI GATC 6 cut(s) 86, 127, 178, 199, 207, 251
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 6 cut(s) 83, 124, 175, 196, 204, 248
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstNI CCWGG 1 cut(s) 10
BstPAI GACNNNNGTC 1 cut(s) 213
BstSCI CCNGG 1 cut(s) 8
BstUI CGCG 1 cut(s) 65
BstV1I GCAGC 3 cut(s) 61, 175, 178
BstX2I RGATCY 4 cut(s) 83, 124, 175, 248
BstYI RGATCY 4 cut(s) 83, 124, 175, 248
Bsu15I ATCGAT 1 cut(s) 199
BsuRI GGCC 1 cut(s) 14
BsuTUI ATCGAT 1 cut(s) 199
BtgI CCRYGG 1 cut(s) 252
BtgZI GCGATG 1 cut(s) 49
BtsCI GGATG 2 cut(s) 57, 269
Cac8I GCNNGC 1 cut(s) 16
CaiI CAGNNNCTG 1 cut(s) 240
CfoI GCGC 1 cut(s) 65
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 2 cut(s) 12, 113
ClaI ATCGAT 1 cut(s) 199
CseI GACGC 2 cut(s) 108, 142
CspAI ACCGGT 1 cut(s) 110
CviAII CATG 2 cut(s) 22, 253
CviJI RGCY 3 cut(s) 14, 74, 97
CviKI_1 RGCY 3 cut(s) 14, 74, 97
DdeI CTNAG 2 cut(s) 98, 128
DpnI GATC 6 cut(s) 85, 126, 177, 198, 206, 250
DpnII GATC 6 cut(s) 83, 124, 175, 196, 204, 248
Eco130I CCWWGG 1 cut(s) 252
Eco31I GGTCTC 1 cut(s) 101
Eco47I GGWCC 1 cut(s) 113
EcoRII CCWGG 1 cut(s) 8
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 2 cut(s) 25, 256
FaiI YATR 2 cut(s) 23, 254
FatI CATG 2 cut(s) 21, 252
FauI CCCGC 1 cut(s) 165
FblI GTMKAC 1 cut(s) 274
Fnu4HI GCNGC 4 cut(s) 19, 75, 189, 192
FokI GGATG 2 cut(s) 64, 276
Fsp4HI GCNGC 4 cut(s) 19, 75, 189, 192
GlaI GCGC 1 cut(s) 64
GluI GCNGC 4 cut(s) 19, 75, 189, 192
HaeIII GGCC 1 cut(s) 14
HapII CCGG 1 cut(s) 111
HgaI GACGC 2 cut(s) 108, 142
HhaI GCGC 1 cut(s) 65
Hin1II CATG 2 cut(s) 25, 256
Hin6I GCGC 1 cut(s) 63
HinP1I GCGC 1 cut(s) 63
HinfI GANTC 1 cut(s) 44
HpaII CCGG 1 cut(s) 111
Hpy166II GTNNAC 1 cut(s) 275
Hpy188I TCNGA 4 cut(s) 43, 124, 135, 162
Hpy188III TCNNGA 2 cut(s) 81, 261
Hpy8I GTNNAC 1 cut(s) 275
Hpy99I CGWCG 2 cut(s) 124, 158
HpyAV CCTTC 2 cut(s) 53, 157
HpyCH4III ACNGT 3 cut(s) 183, 212, 279
HpyCH4V TGCA 1 cut(s) 77
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpyF3I CTNAG 2 cut(s) 98, 128
Hsp92II CATG 2 cut(s) 25, 256
HspAI GCGC 1 cut(s) 63
Kzo9I GATC 6 cut(s) 83, 124, 175, 196, 204, 248
LpnPI CCDG 6 cut(s) 22, 28, 66, 124, 129, 246
Lsp1109I GCAGC 3 cut(s) 61, 175, 178
LweI GCATC 1 cut(s) 86
MalI GATC 6 cut(s) 85, 126, 177, 198, 206, 250
MboI GATC 6 cut(s) 83, 124, 175, 196, 204, 248
MflI RGATCY 4 cut(s) 83, 124, 175, 248
MlyI GAGTC 1 cut(s) 38
MmeI TCCRAC 3 cut(s) 102, 113, 140
MnlI CCTC 3 cut(s) 49, 93, 136
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 1 cut(s) 10
MvaI CCWGG 1 cut(s) 10
MvnI CGCG 1 cut(s) 65
MwoI GCNNNNNNNGC 1 cut(s) 71
NcoI CCATGG 1 cut(s) 252
NdeII GATC 6 cut(s) 83, 124, 175, 196, 204, 248
NlaIII CATG 2 cut(s) 25, 256
PinAI ACCGGT 1 cut(s) 110
PkrI GCNGC 4 cut(s) 20, 76, 190, 193
PleI GAGTC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 38
PshAI GACNNNNGTC 1 cut(s) 213
Psp6I CCWGG 1 cut(s) 8
PspGI CCWGG 1 cut(s) 8
PspPI GGNCC 2 cut(s) 12, 113
PstNI CAGNNNCTG 1 cut(s) 240
PsuI RGATCY 4 cut(s) 83, 124, 175, 248
SatI GCNGC 4 cut(s) 19, 75, 189, 192
Sau3AI GATC 6 cut(s) 83, 124, 175, 196, 204, 248
Sau96I GGNCC 2 cut(s) 12, 113
SchI GAGTC 1 cut(s) 38
ScrFI CCNGG 1 cut(s) 10
SetI ASST 3 cut(s) 104, 147, 168
SfaNI GCATC 1 cut(s) 86
SinI GGWCC 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
SsiI CCGC 4 cut(s) 18, 65, 172, 194
StyD4I CCNGG 1 cut(s) 8
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 3 cut(s) 183, 212, 279
TaqI TCGA 2 cut(s) 199, 207
TaqII GACCGA 1 cut(s) 146
TauI GCSGC 1 cut(s) 21
TseI GCWGC 3 cut(s) 74, 188, 191
VpaK11BI GGWCC 1 cut(s) 113
XmiI GTMKAC 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.