pycom13g05790

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000749
Physical Location & Seq
Reverse (-)
3806657 .. 3806952
296 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g05790.2

Sequence Viewer

Length: 219 bp
ATGGATCGGCTTTCATCAGCTTTCTCTTCCGGGGGTTACGGGGACAAAGACTACGATGATCAATACGATGGCCAAAGAAAATCCGGGACCGAGGGTCACCACCGGTCCGGAGGTTACGGGGACAAAGACCAAGACGATCAATATGGTGGCGGGAGAAAATCCGCGACTGAGGGGTCACCACCGGTCCGGAGGATACGGGGACAAATACCAGGACGATGA

Protein Analysis

73

Amino Acids

7.83

Weight (kDa)

6.71

Isoelectric Point (pI)

48.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 172
AccII CGCG 1 cut(s) 164
AccIII TCCGGA 2 cut(s) 107, 186
AciI CCGC 2 cut(s) 150, 162
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 70
AgeI ACCGGT 2 cut(s) 102, 181
AhdI GACNNNNNGTC 1 cut(s) 93
AjnI CCWGG 1 cut(s) 208
AluBI AGCT 1 cut(s) 20
AluI AGCT 1 cut(s) 20
AlwI GGATC 1 cut(s) 12
Aor13HI TCCGGA 2 cut(s) 107, 186
AoxI GGCC 1 cut(s) 70
AsiGI ACCGGT 2 cut(s) 102, 181
AspS9I GGNCC 3 cut(s) 87, 105, 184
AsuC2I CCSGG 2 cut(s) 31, 85
AsuHPI GGTGA 2 cut(s) 89, 168
AvaII GGWCC 3 cut(s) 87, 105, 184
BalI TGGCCA 1 cut(s) 72
BccI CCATC 1 cut(s) 62
BciT130I CCWGG 1 cut(s) 210
BciVI GTATCC 1 cut(s) 186
BclI TGATCA 1 cut(s) 58
BcnI CCSGG 2 cut(s) 31, 85
BfuI GTATCC 1 cut(s) 186
Bme1390I CCNGG 3 cut(s) 31, 85, 210
Bme18I GGWCC 3 cut(s) 87, 105, 184
BmeRI GACNNNNNGTC 1 cut(s) 93
BmgT120I GGNCC 3 cut(s) 87, 105, 184
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 3 cut(s) 31, 85, 210
BpuMI CCSGG 2 cut(s) 31, 85
BsaJI CCNNGG 2 cut(s) 30, 90
BsaWI WCCGGW 4 cut(s) 102, 107, 181, 186
Bse118I RCCGGY 2 cut(s) 102, 181
BseAI TCCGGA 2 cut(s) 107, 186
BseBI CCWGG 1 cut(s) 210
BseDI CCNNGG 2 cut(s) 30, 90
BseMII CTCAG 1 cut(s) 159
Bsh1236I CGCG 1 cut(s) 164
BshFI GGCC 1 cut(s) 72
BshTI ACCGGT 2 cut(s) 102, 181
BsiSI CCGG 6 cut(s) 30, 84, 103, 108, 182, 187
BslFI GGGAC 4 cut(s) 56, 100, 134, 213
BsmFI GGGAC 4 cut(s) 56, 100, 134, 213
BsnI GGCC 1 cut(s) 72
Bsp13I TCCGGA 2 cut(s) 107, 186
Bsp143I GATC 3 cut(s) 4, 58, 136
BspACI CCGC 2 cut(s) 150, 162
BspANI GGCC 1 cut(s) 72
BspCNI CTCAG 1 cut(s) 160
BspEI TCCGGA 2 cut(s) 107, 186
BspFNI CGCG 1 cut(s) 164
BspLI GGNNCC 1 cut(s) 88
BspPI GGATC 1 cut(s) 12
BsrFI RCCGGY 2 cut(s) 102, 181
BssAI RCCGGY 2 cut(s) 102, 181
BssECI CCNNGG 2 cut(s) 30, 90
BssMI GATC 3 cut(s) 4, 58, 136
Bst2UI CCWGG 1 cut(s) 210
Bst6I CTCTTC 1 cut(s) 31
BstDEI CTNAG 1 cut(s) 168
BstEII GGTNACC 2 cut(s) 95, 174
BstFNI CGCG 1 cut(s) 164
BstKTI GATC 3 cut(s) 7, 61, 139
BstMBI GATC 3 cut(s) 4, 58, 136
BstNI CCWGG 1 cut(s) 210
BstPI GGTNACC 2 cut(s) 95, 174
BstSCI CCNGG 3 cut(s) 29, 83, 208
BstUI CGCG 1 cut(s) 164
BsuI GTATCC 1 cut(s) 186
BsuRI GGCC 1 cut(s) 72
Cfr10I RCCGGY 2 cut(s) 102, 181
Cfr13I GGNCC 3 cut(s) 87, 105, 184
CpoI CGGWCCG 2 cut(s) 105, 184
CspAI ACCGGT 2 cut(s) 102, 181
CspI CGGWCCG 2 cut(s) 105, 184
CviJI RGCY 3 cut(s) 10, 20, 72
CviKI_1 RGCY 3 cut(s) 10, 20, 72
DdeI CTNAG 1 cut(s) 168
DpnI GATC 3 cut(s) 6, 60, 138
DpnII GATC 3 cut(s) 4, 58, 136
DrdI GACNNNNNNGTC 1 cut(s) 172
DriI GACNNNNNGTC 1 cut(s) 93
DseDI GACNNNNNNGTC 1 cut(s) 172
EaeI YGGCCR 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 31
Eam1105I GACNNNNNGTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 31
Eco47I GGWCC 3 cut(s) 87, 105, 184
Eco91I GGTNACC 2 cut(s) 95, 174
EcoO65I GGTNACC 2 cut(s) 95, 174
EcoRII CCWGG 1 cut(s) 208
FaiI YATR 1 cut(s) 144
FaqI GGGAC 4 cut(s) 56, 100, 134, 213
FauI CCCGC 1 cut(s) 143
FbaI TGATCA 1 cut(s) 58
HaeIII GGCC 1 cut(s) 72
HapII CCGG 6 cut(s) 30, 84, 103, 108, 182, 187
HpaII CCGG 6 cut(s) 30, 84, 103, 108, 182, 187
HphI GGTGA 2 cut(s) 89, 168
Hpy188III TCNNGA 2 cut(s) 108, 187
HpyF3I CTNAG 1 cut(s) 168
Kpn2I TCCGGA 2 cut(s) 107, 186
Ksp22I TGATCA 1 cut(s) 58
Kzo9I GATC 3 cut(s) 4, 58, 136
LpnPI CCDG 7 cut(s) 43, 97, 116, 121, 195, 195, 200
MaeIII GTNAC 4 cut(s) 35, 95, 113, 174
MalI GATC 3 cut(s) 6, 60, 138
MboI GATC 3 cut(s) 4, 58, 136
MboII GAAGA 1 cut(s) 18
MlsI TGGCCA 1 cut(s) 72
MluNI TGGCCA 1 cut(s) 72
MnlI CCTC 4 cut(s) 85, 104, 163, 183
Mox20I TGGCCA 1 cut(s) 72
MroI TCCGGA 2 cut(s) 107, 186
MscI TGGCCA 1 cut(s) 72
Msp20I TGGCCA 1 cut(s) 72
MspI CCGG 6 cut(s) 30, 84, 103, 108, 182, 187
MspR9I CCNGG 3 cut(s) 31, 85, 210
MvaI CCWGG 1 cut(s) 210
MvnI CGCG 1 cut(s) 164
NciI CCSGG 2 cut(s) 31, 85
NdeII GATC 3 cut(s) 4, 58, 136
NlaIV GGNNCC 1 cut(s) 88
NmuCI GTSAC 2 cut(s) 95, 174
PfoI TCCNGGA 1 cut(s) 83
PinAI ACCGGT 2 cut(s) 102, 181
Psp6I CCWGG 1 cut(s) 208
PspEI GGTNACC 2 cut(s) 95, 174
PspGI CCWGG 1 cut(s) 208
PspN4I GGNNCC 1 cut(s) 88
PspPI GGNCC 3 cut(s) 87, 105, 184
Rsr2I CGGWCCG 2 cut(s) 105, 184
RsrII CGGWCCG 2 cut(s) 105, 184
Sau3AI GATC 3 cut(s) 4, 58, 136
Sau96I GGNCC 3 cut(s) 87, 105, 184
ScrFI CCNGG 3 cut(s) 31, 85, 210
SetI ASST 2 cut(s) 22, 115
SinI GGWCC 3 cut(s) 87, 105, 184
SsiI CCGC 2 cut(s) 150, 162
StyD4I CCNGG 3 cut(s) 29, 83, 208
TaqII GACCGA 1 cut(s) 104
TseFI GTSAC 2 cut(s) 95, 174
Tsp45I GTSAC 2 cut(s) 95, 174
VpaK11BI GGWCC 3 cut(s) 87, 105, 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.