RLG00000027359

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000749
Physical Location & Seq
Reverse (-)
10895955 .. 10898672
2718 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027359

Sequence Viewer

Length: 591 bp
ATGGCTGCATCTGGATCTGGGGAGTGGCTGAGGAGTGAGTCTGGGCCAGCGTTGGATCGTGGTCGGATTAGGGAGCGTCTGACGCTGATCGGAAGATCCATGGGTTTTGGGTGTTTGGATCTACGGAGTTGTGCGAGAATAGGCTGGCGGGGGAGCGCTGGTGCTGGGGATCCGACGCTGGTGGTGGAGAGGCAGGAGTGGTGTGGATGCAACGGTGCAGATGCGATGGCTGTTAGGGATCTTCGGTGTGAAGAGAAACGGGGCCAACTCGGGAAAGGAGAAGTCGGAGGCAAGGTGGACTCCCGTGATCTTTTCCTCTCGACCGGCGGCGGTCGGCAACGGCGTTGGCAGTTCTGTCTCGCTGTTTTTATATCGTTTGCTGGACGGGAATGGTTTTGGGCCATCGACATCATCTCAAGGGAGGCGATTCACGACGTGAGGTGGGTTTCGGCGGCAGGGATGGCAACTTCGGCAGGAGTTGGTAGCGGCTTGAGACGGCGACCTAAGTTTGGTTGGCTTGCTCAGGCTGTATCAATGCTTTCTGGTGATTGTTTCAGGCATCGTGGTTCTGGCGTGCTTGCGGCGGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

21.21

Weight (kDa)

9.86

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 7 cut(s) 148, 327, 330, 452, 486, 581, 584
AclWI GGATC 7 cut(s) 22, 63, 90, 126, 164, 177, 246
AdeI CACNNNGTG 1 cut(s) 436
AfeI AGCGCT 1 cut(s) 157
AfiI CCNNNNNNNGG 1 cut(s) 509
AjiI CACGTC 1 cut(s) 436
Alw26I GTCTC 2 cut(s) 362, 487
AlwI GGATC 7 cut(s) 22, 63, 90, 126, 164, 177, 246
Ama87I CYCGRG 1 cut(s) 269
Aor51HI AGCGCT 1 cut(s) 157
AoxI GGCC 3 cut(s) 44, 262, 399
ApeKI GCWGC 1 cut(s) 5
AspLEI GCGC 1 cut(s) 158
AspS9I GGNCC 3 cut(s) 44, 262, 399
AsuHPI GGTGA 1 cut(s) 557
AvaI CYCGRG 1 cut(s) 269
BamHI GGATCC 1 cut(s) 169
BbvCI CCTCAGC 1 cut(s) 29
BccI CCATC 3 cut(s) 220, 410, 454
BceAI ACGGC 2 cut(s) 356, 512
BcoDI GTCTC 2 cut(s) 362, 487
BfoI RGCGCY 1 cut(s) 159
BisI GCNGC 6 cut(s) 6, 328, 453, 487, 582, 585
BlsI GCNGC 6 cut(s) 7, 329, 454, 488, 583, 586
BmeT110I CYCGRG 1 cut(s) 269
BmgBI CACGTC 1 cut(s) 436
BmgT120I GGNCC 3 cut(s) 44, 262, 399
BmiI GGNNCC 2 cut(s) 171, 263
BmsI GCATC 4 cut(s) 17, 197, 211, 568
Bpu10I CCTNAGC 2 cut(s) 29, 522
BpuEI CTTGAG 2 cut(s) 400, 511
BsaJI CCNNGG 1 cut(s) 99
BsaXI ACNNNNNCTCC 4 cut(s) 145, 175, 279, 309
Bsc4I CCNNNNNNNGG 1 cut(s) 509
Bse118I RCCGGY 1 cut(s) 323
BseDI CCNNGG 1 cut(s) 99
BseGI GGATG 2 cut(s) 212, 465
BseLI CCNNNNNNNGG 1 cut(s) 509
BseMII CTCAG 2 cut(s) 20, 536
BseRI GAGGAG 1 cut(s) 46
BseYI CCCAGC 1 cut(s) 164
BsgI GTGCAG 1 cut(s) 237
Bsh1285I CGRYCG 2 cut(s) 324, 334
BshFI GGCC 3 cut(s) 46, 264, 401
BsiEI CGRYCG 2 cut(s) 324, 334
BsiHKCI CYCGRG 1 cut(s) 269
BsiSI CCGG 1 cut(s) 324
BslI CCNNNNNNNGG 1 cut(s) 509
BsmAI GTCTC 2 cut(s) 362, 487
BsmBI CGTCTC 1 cut(s) 487
BsnI GGCC 3 cut(s) 46, 264, 401
BsoBI CYCGRG 1 cut(s) 269
Bsp143I GATC 8 cut(s) 14, 55, 87, 95, 118, 169, 238, 307
Bsp19I CCATGG 1 cut(s) 99
BspACI CCGC 7 cut(s) 148, 327, 330, 452, 486, 581, 584
BspANI GGCC 3 cut(s) 46, 264, 401
BspCNI CTCAG 2 cut(s) 21, 535
BspLI GGNNCC 2 cut(s) 171, 263
BspPI GGATC 7 cut(s) 22, 63, 90, 126, 164, 177, 246
BsrFI RCCGGY 1 cut(s) 323
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 8 cut(s) 14, 55, 87, 95, 118, 169, 238, 307
BssT1I CCWWGG 1 cut(s) 99
Bst4CI ACNGT 1 cut(s) 215
Bst6I CTCTTC 1 cut(s) 246
BstC8I GCNNGC 5 cut(s) 48, 146, 519, 575, 579
BstDEI CTNAG 3 cut(s) 29, 504, 522
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 2 cut(s) 212, 465
BstH2I RGCGCY 1 cut(s) 159
BstHHI GCGC 1 cut(s) 158
BstKTI GATC 8 cut(s) 17, 58, 90, 98, 121, 172, 241, 310
BstMAI GTCTC 2 cut(s) 362, 487
BstMBI GATC 8 cut(s) 14, 55, 87, 95, 118, 169, 238, 307
BstMCI CGRYCG 2 cut(s) 324, 334
BstMWI GCNNNNNNNGC 3 cut(s) 82, 461, 470
BstX2I RGATCY 5 cut(s) 14, 95, 118, 169, 238
BstYI RGATCY 5 cut(s) 14, 95, 118, 169, 238
BsuRI GGCC 3 cut(s) 46, 264, 401
BtgI CCRYGG 1 cut(s) 99
BtgZI GCGATG 1 cut(s) 239
BtrI CACGTC 1 cut(s) 436
BtsCI GGATG 2 cut(s) 212, 465
Cac8I GCNNGC 5 cut(s) 48, 146, 519, 575, 579
CfoI GCGC 1 cut(s) 158
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 3 cut(s) 44, 262, 399
CseI GACGC 3 cut(s) 65, 91, 184
CviAII CATG 1 cut(s) 100
DdeI CTNAG 3 cut(s) 29, 504, 522
DpnI GATC 8 cut(s) 16, 57, 89, 97, 120, 171, 240, 309
DpnII GATC 8 cut(s) 14, 55, 87, 95, 118, 169, 238, 307
DraIII CACNNNGTG 1 cut(s) 436
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 99
Eco47III AGCGCT 1 cut(s) 157
Eco88I CYCGRG 1 cut(s) 269
EcoT14I CCWWGG 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 99
Esp3I CGTCTC 1 cut(s) 487
FaeI CATG 1 cut(s) 103
FaiI YATR 2 cut(s) 101, 371
FatI CATG 1 cut(s) 99
FauI CCCGC 1 cut(s) 141
Fnu4HI GCNGC 6 cut(s) 6, 328, 453, 487, 582, 585
FokI GGATG 2 cut(s) 219, 472
Fsp4HI GCNGC 6 cut(s) 6, 328, 453, 487, 582, 585
GlaI GCGC 1 cut(s) 157
GluI GCNGC 6 cut(s) 6, 328, 453, 487, 582, 585
GsaI CCCAGC 1 cut(s) 168
HaeII RGCGCY 1 cut(s) 159
HaeIII GGCC 3 cut(s) 46, 264, 401
HapII CCGG 1 cut(s) 324
HgaI GACGC 3 cut(s) 65, 91, 184
HhaI GCGC 1 cut(s) 158
Hin1II CATG 1 cut(s) 103
Hin6I GCGC 1 cut(s) 156
HinP1I GCGC 1 cut(s) 156
HinfI GANTC 3 cut(s) 38, 299, 427
HpaII CCGG 1 cut(s) 324
HphI GGTGA 1 cut(s) 557
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 5 cut(s) 66, 81, 92, 174, 287
Hpy188III TCNNGA 4 cut(s) 12, 271, 319, 431
Hpy8I GTNNAC 1 cut(s) 298
Hpy99I CGWCG 2 cut(s) 178, 437
HpyCH4III ACNGT 1 cut(s) 215
HpyCH4IV ACGT 1 cut(s) 435
HpyCH4V TGCA 3 cut(s) 8, 210, 218
HpyF10VI GCNNNNNNNGC 3 cut(s) 82, 461, 470
HpyF3I CTNAG 3 cut(s) 29, 504, 522
HpySE526I ACGT 1 cut(s) 435
Hsp92II CATG 1 cut(s) 103
HspAI GCGC 1 cut(s) 156
Kzo9I GATC 8 cut(s) 14, 55, 87, 95, 118, 169, 238, 307
LmnI GCTCC 2 cut(s) 73, 153
LweI GCATC 4 cut(s) 17, 197, 211, 568
MaeII ACGT 1 cut(s) 435
MalI GATC 8 cut(s) 16, 57, 89, 97, 120, 171, 240, 309
MboI GATC 8 cut(s) 14, 55, 87, 95, 118, 169, 238, 307
MboII GAAGA 3 cut(s) 105, 233, 263
MflI RGATCY 5 cut(s) 14, 95, 118, 169, 238
MlyI GAGTC 2 cut(s) 47, 293
MmeI TCCRAC 4 cut(s) 33, 44, 197, 265
MnlI CCTC 6 cut(s) 24, 183, 281, 326, 415, 432
MspI CCGG 1 cut(s) 324
MwoI GCNNNNNNNGC 3 cut(s) 82, 461, 470
NcoI CCATGG 1 cut(s) 99
NdeII GATC 8 cut(s) 14, 55, 87, 95, 118, 169, 238, 307
NlaIII CATG 1 cut(s) 103
NlaIV GGNNCC 2 cut(s) 171, 263
PcsI WCGNNNNNNNCGW 1 cut(s) 340
PfeI GAWTC 1 cut(s) 427
PkrI GCNGC 6 cut(s) 7, 329, 454, 488, 583, 586
PleI GAGTC 2 cut(s) 46, 293
PpsI GAGTC 2 cut(s) 46, 293
PspFI CCCAGC 1 cut(s) 164
PspN4I GGNNCC 2 cut(s) 171, 263
PspPI GGNCC 3 cut(s) 44, 262, 399
PsuI RGATCY 5 cut(s) 14, 95, 118, 169, 238
SatI GCNGC 6 cut(s) 6, 328, 453, 487, 582, 585
Sau3AI GATC 8 cut(s) 14, 55, 87, 95, 118, 169, 238, 307
Sau96I GGNCC 3 cut(s) 44, 262, 399
SchI GAGTC 2 cut(s) 47, 293
SetI ASST 4 cut(s) 297, 438, 443, 505
SfaNI GCATC 4 cut(s) 17, 197, 211, 568
SmlI CTYRAG 2 cut(s) 415, 490
SmoI CTYRAG 2 cut(s) 415, 490
SsiI CCGC 7 cut(s) 148, 327, 330, 452, 486, 581, 584
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 1 cut(s) 215
TaiI ACGT 1 cut(s) 438
TaqI TCGA 2 cut(s) 320, 405
TauI GCSGC 5 cut(s) 330, 455, 489, 584, 587
TfiI GAWTC 1 cut(s) 427
TseI GCWGC 1 cut(s) 5
TspGWI ACGGA 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.