RLG00000024858

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000749
Physical Location & Seq
Forward (+)
39835489 .. 39837225
1737 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024858

Sequence Viewer

Length: 318 bp
ATGGCTGCATCTGGATCTGTGGCGTGGCTGGGGATTGAGACCGGTCCAGCATTGGATCTTAGTCGGATTGGGGAGGCGTCTTGCGCCTGTCGGAAGGTGGGGCGGGATCTACCGTTTGCTGTTGCGGATCGGTGGATCGATGGCGATCCTTTGTTGTTTGCATTCGGGTCCTATCGTAGATCCATGGGTTTTGGATGTTTGGGTCTACGGAGGTGCGCGAGGACAGCCTTGGTGGATCGCGGTGCTGCCTTGGTGGATTGCGGCGCTGGAGTCTTGCGGGGAGTGGTGGTTGGGCCGGGCCTTTGTGTTAGGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

106

Amino Acids

10.87

Weight (kDa)

8.95

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 205
AccII CGCG 2 cut(s) 218, 240
AciI CCGC 5 cut(s) 103, 125, 240, 261, 277
AclWI GGATC 8 cut(s) 22, 63, 114, 135, 140, 143, 174, 243
AcyI GRCGYC 1 cut(s) 77
AgeI ACCGGT 1 cut(s) 41
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 8 cut(s) 22, 63, 114, 135, 140, 143, 174, 243
AoxI GGCC 3 cut(s) 293, 298, 312
ApeKI GCWGC 2 cut(s) 5, 245
AsiGI ACCGGT 1 cut(s) 41
AspLEI GCGC 3 cut(s) 86, 218, 266
AspS9I GGNCC 5 cut(s) 44, 168, 293, 298, 312
AsuC2I CCSGG 1 cut(s) 297
AvaII GGWCC 2 cut(s) 44, 168
BbvI GCAGC 1 cut(s) 232
BccI CCATC 1 cut(s) 134
BcnI CCSGG 1 cut(s) 297
BcoDI GTCTC 1 cut(s) 32
BfaI CTAG 1 cut(s) 316
BfoI RGCGCY 1 cut(s) 267
BisI GCNGC 3 cut(s) 6, 246, 262
BlsI GCNGC 3 cut(s) 7, 247, 263
Bme1390I CCNGG 1 cut(s) 297
Bme18I GGWCC 2 cut(s) 44, 168
BmgT120I GGNCC 5 cut(s) 44, 168, 293, 298, 312
BmiI GGNNCC 1 cut(s) 169
BmrFI CCNGG 1 cut(s) 297
BmsI GCATC 1 cut(s) 17
BpmI CTGGAG 1 cut(s) 288
BpuMI CCSGG 1 cut(s) 297
Bsa29I ATCGAT 1 cut(s) 138
BsaBI GATNNNNATC 1 cut(s) 144
BsaHI GRCGYC 1 cut(s) 77
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 3 cut(s) 183, 228, 249
BsaWI WCCGGW 1 cut(s) 41
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bse118I RCCGGY 1 cut(s) 41
Bse8I GATNNNNATC 1 cut(s) 144
BseCI ATCGAT 1 cut(s) 138
BseDI CCNNGG 3 cut(s) 183, 228, 249
BseGI GGATG 1 cut(s) 200
BseJI GATNNNNATC 1 cut(s) 144
BseXI GCAGC 1 cut(s) 232
BseYI CCCAGC 1 cut(s) 28
Bsh1236I CGCG 2 cut(s) 218, 240
BshFI GGCC 3 cut(s) 295, 300, 314
BshTI ACCGGT 1 cut(s) 41
BshVI ATCGAT 1 cut(s) 138
BsiSI CCGG 2 cut(s) 42, 296
BsmAI GTCTC 1 cut(s) 32
BsmI GAATGC 1 cut(s) 161
BsnI GGCC 3 cut(s) 295, 300, 314
Bso31I GGTCTC 1 cut(s) 32
Bsp143I GATC 8 cut(s) 14, 55, 106, 127, 135, 145, 179, 235
Bsp19I CCATGG 1 cut(s) 183
BspACI CCGC 5 cut(s) 103, 125, 240, 261, 277
BspANI GGCC 3 cut(s) 295, 300, 314
BspDI ATCGAT 1 cut(s) 138
BspFNI CGCG 2 cut(s) 218, 240
BspLI GGNNCC 1 cut(s) 169
BspPI GGATC 8 cut(s) 22, 63, 114, 135, 140, 143, 174, 243
BspTNI GGTCTC 1 cut(s) 32
BsrFI RCCGGY 1 cut(s) 41
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 3 cut(s) 183, 228, 249
BssMI GATC 8 cut(s) 14, 55, 106, 127, 135, 145, 179, 235
BssNI GRCGYC 1 cut(s) 77
BssT1I CCWWGG 3 cut(s) 183, 228, 249
Bst4CI ACNGT 1 cut(s) 114
BstACI GRCGYC 1 cut(s) 77
BstDEI CTNAG 1 cut(s) 59
BstDSI CCRYGG 1 cut(s) 183
BstF5I GGATG 1 cut(s) 200
BstFNI CGCG 2 cut(s) 218, 240
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 3 cut(s) 86, 218, 266
BstKTI GATC 8 cut(s) 17, 58, 109, 130, 138, 148, 182, 238
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 8 cut(s) 14, 55, 106, 127, 135, 145, 179, 235
BstMWI GCNNNNNNNGC 2 cut(s) 83, 224
BstSCI CCNGG 1 cut(s) 295
BstUI CGCG 2 cut(s) 218, 240
BstV1I GCAGC 1 cut(s) 232
BstX2I RGATCY 4 cut(s) 14, 55, 106, 179
BstYI RGATCY 4 cut(s) 14, 55, 106, 179
Bsu15I ATCGAT 1 cut(s) 138
BsuRI GGCC 3 cut(s) 295, 300, 314
BsuTUI ATCGAT 1 cut(s) 138
BtgI CCRYGG 1 cut(s) 183
BtsCI GGATG 1 cut(s) 200
CfoI GCGC 3 cut(s) 86, 218, 266
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 5 cut(s) 44, 168, 293, 298, 312
ClaI ATCGAT 1 cut(s) 138
CseI GACGC 1 cut(s) 66
CspAI ACCGGT 1 cut(s) 41
CviAII CATG 1 cut(s) 184
CviJI RGCY 6 cut(s) 5, 28, 227, 295, 300, 314
CviKI_1 RGCY 6 cut(s) 5, 28, 227, 295, 300, 314
DdeI CTNAG 1 cut(s) 59
DpnI GATC 8 cut(s) 16, 57, 108, 129, 137, 147, 181, 237
DpnII GATC 8 cut(s) 14, 55, 106, 127, 135, 145, 179, 235
Eco130I CCWWGG 3 cut(s) 183, 228, 249
Eco31I GGTCTC 1 cut(s) 32
Eco47I GGWCC 2 cut(s) 44, 168
EcoO109I RGGNCCY 2 cut(s) 168, 312
EcoT14I CCWWGG 3 cut(s) 183, 228, 249
ErhI CCWWGG 3 cut(s) 183, 228, 249
FaeI CATG 1 cut(s) 187
FaiI YATR 1 cut(s) 185
FatI CATG 1 cut(s) 183
FauI CCCGC 2 cut(s) 96, 270
FblI GTMKAC 1 cut(s) 205
Fnu4HI GCNGC 3 cut(s) 6, 246, 262
FokI GGATG 1 cut(s) 207
Fsp4HI GCNGC 3 cut(s) 6, 246, 262
FspBI CTAG 1 cut(s) 316
GlaI GCGC 3 cut(s) 85, 217, 265
GluI GCNGC 3 cut(s) 6, 246, 262
GsaI CCCAGC 1 cut(s) 32
GsuI CTGGAG 1 cut(s) 288
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 3 cut(s) 295, 300, 314
HapII CCGG 2 cut(s) 42, 296
HgaI GACGC 1 cut(s) 66
HhaI GCGC 3 cut(s) 86, 218, 266
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 1 cut(s) 187
Hin6I GCGC 3 cut(s) 84, 216, 264
HinP1I GCGC 3 cut(s) 84, 216, 264
HinfI GANTC 1 cut(s) 270
HpaII CCGG 2 cut(s) 42, 296
Hpy166II GTNNAC 1 cut(s) 206
Hpy188I TCNGA 2 cut(s) 66, 93
Hpy188III TCNNGA 1 cut(s) 12
Hpy8I GTNNAC 1 cut(s) 206
HpyAV CCTTC 1 cut(s) 88
HpyCH4III ACNGT 1 cut(s) 114
HpyCH4V TGCA 2 cut(s) 8, 161
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 224
HpyF3I CTNAG 1 cut(s) 59
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 1 cut(s) 187
HspAI GCGC 3 cut(s) 84, 216, 264
Kzo9I GATC 8 cut(s) 14, 55, 106, 127, 135, 145, 179, 235
LpnPI CCDG 6 cut(s) 14, 55, 60, 100, 252, 309
Lsp1109I GCAGC 1 cut(s) 232
LweI GCATC 1 cut(s) 17
MaeI CTAG 1 cut(s) 316
MalI GATC 8 cut(s) 16, 57, 108, 129, 137, 147, 181, 237
MboI GATC 8 cut(s) 14, 55, 106, 127, 135, 145, 179, 235
MflI RGATCY 4 cut(s) 14, 55, 106, 179
MlyI GAGTC 1 cut(s) 279
MmeI TCCRAC 2 cut(s) 44, 71
MnlI CCTC 3 cut(s) 67, 204, 213
MspI CCGG 2 cut(s) 42, 296
MspR9I CCNGG 1 cut(s) 297
Mva1269I GAATGC 1 cut(s) 161
MvnI CGCG 2 cut(s) 218, 240
MwoI GCNNNNNNNGC 2 cut(s) 83, 224
NciI CCSGG 1 cut(s) 297
NcoI CCATGG 1 cut(s) 183
NdeII GATC 8 cut(s) 14, 55, 106, 127, 135, 145, 179, 235
NlaIII CATG 1 cut(s) 187
NlaIV GGNNCC 1 cut(s) 169
PctI GAATGC 1 cut(s) 161
PinAI ACCGGT 1 cut(s) 41
PkrI GCNGC 3 cut(s) 7, 247, 263
PleI GAGTC 1 cut(s) 278
PpsI GAGTC 1 cut(s) 278
PpuMI RGGWCCY 1 cut(s) 168
Psp5II RGGWCCY 1 cut(s) 168
PspFI CCCAGC 1 cut(s) 28
PspN4I GGNNCC 1 cut(s) 169
PspPI GGNCC 5 cut(s) 44, 168, 293, 298, 312
PspPPI RGGWCCY 1 cut(s) 168
PsuI RGATCY 4 cut(s) 14, 55, 106, 179
SatI GCNGC 3 cut(s) 6, 246, 262
Sau3AI GATC 8 cut(s) 14, 55, 106, 127, 135, 145, 179, 235
Sau96I GGNCC 5 cut(s) 44, 168, 293, 298, 312
SchI GAGTC 1 cut(s) 279
ScrFI CCNGG 1 cut(s) 297
SetI ASST 2 cut(s) 99, 215
SfaNI GCATC 1 cut(s) 17
SinI GGWCC 2 cut(s) 44, 168
SsiI CCGC 5 cut(s) 103, 125, 240, 261, 277
SspMI CTAG 1 cut(s) 316
StyD4I CCNGG 1 cut(s) 295
StyI CCWWGG 3 cut(s) 183, 228, 249
TaaI ACNGT 1 cut(s) 114
TaqI TCGA 1 cut(s) 138
TauI GCSGC 1 cut(s) 264
TseI GCWGC 2 cut(s) 5, 245
TspGWI ACGGA 1 cut(s) 223
VpaK11BI GGWCC 2 cut(s) 44, 168
XmiI GTMKAC 1 cut(s) 205
XspI CTAG 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.