Rroxscaffold_5G00374780

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000749
Physical Location & Seq
Reverse (-)
55740359 .. 55744825
4467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00374780.1

Sequence Viewer

Length: 705 bp
ATGATTCGGTCCACGAATGAGCTCGGATCCAAGCGGCATTCCCAAAAGCGAGGACACCTAATTGGCCATCAGCAATGGTTTGAGCCTCGAAGTGATATGGGCTTTAGGCTCGGTTTGAGCACCCAATTAGAGTTGGGCCTCCCAAGCGATATGGGCACCCATCCCGCCTTATCGGCGGCGGCAGCCATTTTCACCTCCTTTAATCGAAAGATAATAGTAAGAAATATCACCGCTTGTAGGAGAACGCGCCTCACGCGCGGAGAAAAACCGAGAGGGAGATCGAGAGTGCTCTCTCCCCGCCGAACGACGCCGGCGCCCCTGCCGCTGCGTGAGTTCGGTCGGGAGAGGATGTTTACTGGGCTTGCGGCATGGTTCTGGGCGATGGTCCGCTCGAGGATGGAGGAAGGCGCGGTGGTGGTTGCATCCGGATTTGTGGCGTGGGCGAGGTTTGAGGCCGGTCTAGCGTCGGATCTTAGTCGGATTGGGGAGGTGTCTTGCATCGGTACGAAGGTGTGGCGGGATCTACGGTTTCTTTGCGCGGATCGGTGGAGCGACGGTGGTCCTTTGTTGTTTGATTTAAGTTTCGTCGTAGATCCATGGGTTTTGGATGTTTGGGTCTACGGTGGTGCGCGGGGTCGGGCGGGCGGGGGAGCGCGGTCTCGGGGATCCGACGGCGGTGGTGGAGCGGCGGGAGTGGTGGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

234

Amino Acids

25.64

Weight (kDa)

11.33

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 155, 313
AccBSI CCGCTC 2 cut(s) 390, 686
AccI GTMKAC 1 cut(s) 618
AccII CGCG 7 cut(s) 247, 256, 258, 410, 539, 631, 655
AccIII TCCGGA 1 cut(s) 425
AclWI GGATC 8 cut(s) 21, 34, 477, 528, 549, 587, 660, 673
AcoI YGGCCR 1 cut(s) 64
AcyI GRCGYC 2 cut(s) 308, 314
AfaI GTAC 1 cut(s) 505
AfiI CCNNNNNNNGG 1 cut(s) 237
AluBI AGCT 1 cut(s) 22
AluI AGCT 1 cut(s) 22
Alw21I GWGCWC 3 cut(s) 24, 122, 291
Alw26I GTCTC 1 cut(s) 663
AlwI GGATC 8 cut(s) 21, 34, 477, 528, 549, 587, 660, 673
Ama87I CYCGRG 2 cut(s) 391, 660
Aor13HI TCCGGA 1 cut(s) 425
AoxI GGCC 3 cut(s) 64, 136, 453
ApeKI GCWGC 2 cut(s) 182, 325
AspLEI GCGC 7 cut(s) 249, 258, 316, 410, 539, 631, 655
AspS9I GGNCC 4 cut(s) 9, 136, 385, 560
AsuHPI GGTGA 2 cut(s) 184, 220
AvaI CYCGRG 2 cut(s) 391, 660
AvaII GGWCC 3 cut(s) 9, 385, 560
BaeGI GKGCMC 1 cut(s) 158
BaeI ACNNNNGTAYC 2 cut(s) 495, 528
BalI TGGCCA 1 cut(s) 66
BamHI GGATCC 2 cut(s) 26, 665
BanI GGYRCC 2 cut(s) 155, 313
BanII GRGCYC 1 cut(s) 24
Bbv12I GWGCWC 3 cut(s) 24, 122, 291
BbvI GCAGC 2 cut(s) 194, 312
BccI CCATC 4 cut(s) 75, 168, 376, 391
BceAI ACGGC 1 cut(s) 688
BcoDI GTCTC 1 cut(s) 663
BfaI CTAG 1 cut(s) 461
BfoI RGCGCY 1 cut(s) 317
BglI GCCNNNNNGGC 1 cut(s) 173
BisI GCNGC 8 cut(s) 35, 177, 180, 183, 323, 326, 366, 687
BlsI GCNGC 8 cut(s) 36, 178, 181, 184, 324, 327, 367, 688
Bme18I GGWCC 3 cut(s) 9, 385, 560
BmeT110I CYCGRG 2 cut(s) 391, 660
BmgT120I GGNCC 4 cut(s) 9, 136, 385, 560
BmiI GGNNCC 4 cut(s) 28, 157, 315, 667
BmrI ACTGGG 1 cut(s) 366
BmsI GCATC 2 cut(s) 431, 507
BmuI ACTGGG 1 cut(s) 366
BoxI GACNNNNGTC 1 cut(s) 558
BsaHI GRCGYC 2 cut(s) 308, 314
BsaI GGTCTC 1 cut(s) 663
BsaJI CCNNGG 1 cut(s) 596
BsaWI WCCGGW 1 cut(s) 425
BsaXI ACNNNNNCTCC 4 cut(s) 335, 365, 541, 571
Bsc4I CCNNNNNNNGG 1 cut(s) 237
Bse118I RCCGGY 2 cut(s) 310, 455
Bse1I ACTGG 1 cut(s) 361
Bse3DI GCAATG 1 cut(s) 80
BseAI TCCGGA 1 cut(s) 425
BseDI CCNNGG 1 cut(s) 596
BseGI GGATG 5 cut(s) 160, 354, 402, 422, 613
BseLI CCNNNNNNNGG 1 cut(s) 237
BseMI GCAATG 1 cut(s) 80
BseNI ACTGG 1 cut(s) 361
BseSI GKGCMC 1 cut(s) 158
BseXI GCAGC 2 cut(s) 194, 312
Bsh1236I CGCG 7 cut(s) 247, 256, 258, 410, 539, 631, 655
Bsh1285I CGRYCG 1 cut(s) 340
BshFI GGCC 3 cut(s) 66, 138, 455
BshNI GGYRCC 2 cut(s) 155, 313
BsiEI CGRYCG 1 cut(s) 340
BsiHKAI GWGCWC 3 cut(s) 24, 122, 291
BsiHKCI CYCGRG 2 cut(s) 391, 660
BsiSI CCGG 3 cut(s) 311, 426, 456
BslI CCNNNNNNNGG 1 cut(s) 237
BsmAI GTCTC 1 cut(s) 663
BsmI GAATGC 1 cut(s) 37
BsnI GGCC 3 cut(s) 66, 138, 455
Bso31I GGTCTC 1 cut(s) 663
BsoBI CYCGRG 2 cut(s) 391, 660
Bsp1286I GDGCHC 4 cut(s) 24, 122, 158, 291
Bsp13I TCCGGA 1 cut(s) 425
Bsp143I GATC 7 cut(s) 26, 278, 469, 520, 541, 592, 665
Bsp19I CCATGG 1 cut(s) 596
BspANI GGCC 3 cut(s) 66, 138, 455
BspEI TCCGGA 1 cut(s) 425
BspFNI CGCG 7 cut(s) 247, 256, 258, 410, 539, 631, 655
BspLI GGNNCC 4 cut(s) 28, 157, 315, 667
BspPI GGATC 8 cut(s) 21, 34, 477, 528, 549, 587, 660, 673
BspT107I GGYRCC 2 cut(s) 155, 313
BspTNI GGTCTC 1 cut(s) 663
BsrBI CCGCTC 2 cut(s) 390, 686
BsrDI GCAATG 1 cut(s) 80
BsrFI RCCGGY 2 cut(s) 310, 455
BsrI ACTGG 1 cut(s) 361
BssAI RCCGGY 2 cut(s) 310, 455
BssECI CCNNGG 1 cut(s) 596
BssMI GATC 7 cut(s) 26, 278, 469, 520, 541, 592, 665
BssNI GRCGYC 2 cut(s) 308, 314
BssT1I CCWWGG 1 cut(s) 596
Bst4CI ACNGT 3 cut(s) 528, 557, 623
BstACI GRCGYC 2 cut(s) 308, 314
BstC8I GCNNGC 3 cut(s) 312, 363, 643
BstDEI CTNAG 1 cut(s) 473
BstDSI CCRYGG 1 cut(s) 596
BstF5I GGATG 5 cut(s) 160, 354, 402, 422, 613
BstFNI CGCG 7 cut(s) 247, 256, 258, 410, 539, 631, 655
BstH2I RGCGCY 1 cut(s) 317
BstHHI GCGC 7 cut(s) 249, 258, 316, 410, 539, 631, 655
BstKTI GATC 7 cut(s) 29, 281, 472, 523, 544, 595, 668
BstMAI GTCTC 1 cut(s) 663
BstMBI GATC 7 cut(s) 26, 278, 469, 520, 541, 592, 665
BstMCI CGRYCG 1 cut(s) 340
BstMWI GCNNNNNNNGC 8 cut(s) 144, 153, 173, 182, 253, 255, 322, 461
BstPAI GACNNNNGTC 1 cut(s) 558
BstSLI GKGCMC 1 cut(s) 158
BstUI CGCG 7 cut(s) 247, 256, 258, 410, 539, 631, 655
BstV1I GCAGC 2 cut(s) 194, 312
BstX2I RGATCY 5 cut(s) 26, 469, 520, 592, 665
BstYI RGATCY 5 cut(s) 26, 469, 520, 592, 665
BsuRI GGCC 3 cut(s) 66, 138, 455
BtgI CCRYGG 1 cut(s) 596
BtgZI GCGATG 1 cut(s) 395
BtsCI GGATG 5 cut(s) 160, 354, 402, 422, 613
Cac8I GCNNGC 3 cut(s) 312, 363, 643
CfoI GCGC 7 cut(s) 249, 258, 316, 410, 539, 631, 655
Cfr10I RCCGGY 2 cut(s) 310, 455
Cfr13I GGNCC 4 cut(s) 9, 136, 385, 560
CseI GACGC 2 cut(s) 316, 453
Csp6I GTAC 1 cut(s) 504
CviAII CATG 2 cut(s) 369, 597
CviJI RGCY 9 cut(s) 22, 66, 85, 102, 109, 138, 185, 361, 455
CviKI_1 RGCY 9 cut(s) 22, 66, 85, 102, 109, 138, 185, 361, 455
CviQI GTAC 1 cut(s) 504
DdeI CTNAG 1 cut(s) 473
DinI GGCGCC 1 cut(s) 315
DpnI GATC 7 cut(s) 28, 280, 471, 522, 543, 594, 667
DpnII GATC 7 cut(s) 26, 278, 469, 520, 541, 592, 665
EaeI YGGCCR 1 cut(s) 64
Ecl136II GAGCTC 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 596
Eco24I GRGCYC 1 cut(s) 24
Eco31I GGTCTC 1 cut(s) 663
Eco47I GGWCC 3 cut(s) 9, 385, 560
Eco53kI GAGCTC 1 cut(s) 22
Eco88I CYCGRG 2 cut(s) 391, 660
EcoICRI GAGCTC 1 cut(s) 22
EcoT14I CCWWGG 1 cut(s) 596
EcoT38I GRGCYC 1 cut(s) 24
EgeI GGCGCC 1 cut(s) 315
EheI GGCGCC 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 596
FaeI CATG 2 cut(s) 372, 600
FaiI YATR 4 cut(s) 98, 152, 370, 598
FatI CATG 2 cut(s) 368, 596
FauI CCCGC 7 cut(s) 172, 305, 510, 624, 634, 638, 682
FblI GTMKAC 1 cut(s) 618
Fnu4HI GCNGC 8 cut(s) 35, 177, 180, 183, 323, 326, 366, 687
FokI GGATG 5 cut(s) 147, 361, 409, 409, 620
FriOI GRGCYC 1 cut(s) 24
Fsp4HI GCNGC 8 cut(s) 35, 177, 180, 183, 323, 326, 366, 687
FspBI CTAG 1 cut(s) 461
GlaI GCGC 7 cut(s) 248, 257, 315, 409, 538, 630, 654
GluI GCNGC 8 cut(s) 35, 177, 180, 183, 323, 326, 366, 687
HaeII RGCGCY 1 cut(s) 317
HaeIII GGCC 3 cut(s) 66, 138, 455
HapII CCGG 3 cut(s) 311, 426, 456
HgaI GACGC 2 cut(s) 316, 453
HhaI GCGC 7 cut(s) 249, 258, 316, 410, 539, 631, 655
Hin1I GRCGYC 2 cut(s) 308, 314
Hin1II CATG 2 cut(s) 372, 600
Hin6I GCGC 7 cut(s) 247, 256, 314, 408, 537, 629, 653
HinP1I GCGC 7 cut(s) 247, 256, 314, 408, 537, 629, 653
HinfI GANTC 1 cut(s) 4
HpaII CCGG 3 cut(s) 311, 426, 456
HphI GGTGA 2 cut(s) 184, 220
Hpy166II GTNNAC 3 cut(s) 12, 354, 619
Hpy188I TCNGA 4 cut(s) 26, 469, 480, 670
Hpy188III TCNNGA 3 cut(s) 282, 341, 426
Hpy8I GTNNAC 3 cut(s) 12, 354, 619
Hpy99I CGWCG 5 cut(s) 310, 469, 557, 590, 674
HpyAV CCTTC 2 cut(s) 398, 502
HpyCH4III ACNGT 3 cut(s) 528, 557, 623
HpyCH4V TGCA 2 cut(s) 422, 498
HpyF10VI GCNNNNNNNGC 8 cut(s) 144, 153, 173, 182, 253, 255, 322, 461
HpyF3I CTNAG 1 cut(s) 473
Hsp92I GRCGYC 2 cut(s) 308, 314
Hsp92II CATG 2 cut(s) 372, 600
HspAI GCGC 7 cut(s) 247, 256, 314, 408, 537, 629, 653
KasI GGCGCC 1 cut(s) 313
Kpn2I TCCGGA 1 cut(s) 425
KroI GCCGGC 1 cut(s) 310
KroNI GCCGGC 1 cut(s) 312
Kzo9I GATC 7 cut(s) 26, 278, 469, 520, 541, 592, 665
LmnI GCTCC 3 cut(s) 549, 650, 683
LpnPI CCDG 6 cut(s) 324, 332, 342, 361, 439, 469
Lsp1109I GCAGC 2 cut(s) 194, 312
LweI GCATC 2 cut(s) 431, 507
MaeI CTAG 1 cut(s) 461
MalI GATC 7 cut(s) 28, 280, 471, 522, 543, 594, 667
MbiI CCGCTC 2 cut(s) 390, 686
MboI GATC 7 cut(s) 26, 278, 469, 520, 541, 592, 665
MflI RGATCY 5 cut(s) 26, 469, 520, 592, 665
MhlI GDGCHC 4 cut(s) 24, 122, 158, 291
MlsI TGGCCA 1 cut(s) 66
MluCI AATT 2 cut(s) 60, 125
MluNI TGGCCA 1 cut(s) 66
Mly113I GGCGCC 1 cut(s) 314
MmeI TCCRAC 3 cut(s) 447, 458, 693
Mox20I TGGCCA 1 cut(s) 66
MreI CGCCGGCG 1 cut(s) 310
MroI TCCGGA 1 cut(s) 425
MroNI GCCGGC 1 cut(s) 310
MscI TGGCCA 1 cut(s) 66
MseI TTAA 2 cut(s) 201, 578
Msp20I TGGCCA 1 cut(s) 66
MspA1I CMGCKG 1 cut(s) 325
MspI CCGG 3 cut(s) 311, 426, 456
Mva1269I GAATGC 1 cut(s) 37
MvnI CGCG 7 cut(s) 247, 256, 258, 410, 539, 631, 655
MwoI GCNNNNNNNGC 8 cut(s) 144, 153, 173, 182, 253, 255, 322, 461
NaeI GCCGGC 1 cut(s) 312
NarI GGCGCC 1 cut(s) 314
NcoI CCATGG 1 cut(s) 596
NdeII GATC 7 cut(s) 26, 278, 469, 520, 541, 592, 665
NgoMIV GCCGGC 1 cut(s) 310
NlaIII CATG 2 cut(s) 372, 600
NlaIV GGNNCC 4 cut(s) 28, 157, 315, 667
PaeR7I CTCGAG 1 cut(s) 391
PctI GAATGC 1 cut(s) 37
PdiI GCCGGC 1 cut(s) 312
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 8 cut(s) 36, 178, 181, 184, 324, 327, 367, 688
PluTI GGCGCC 1 cut(s) 317
PshAI GACNNNNGTC 1 cut(s) 558
Psp124BI GAGCTC 1 cut(s) 24
PspN4I GGNNCC 4 cut(s) 28, 157, 315, 667
PspPI GGNCC 4 cut(s) 9, 136, 385, 560
PspXI VCTCGAGB 1 cut(s) 391
PsuI RGATCY 5 cut(s) 26, 469, 520, 592, 665
RsaI GTAC 1 cut(s) 505
RsaNI GTAC 1 cut(s) 504
SacI GAGCTC 1 cut(s) 24
SaqAI TTAA 2 cut(s) 201, 578
SatI GCNGC 8 cut(s) 35, 177, 180, 183, 323, 326, 366, 687
Sau3AI GATC 7 cut(s) 26, 278, 469, 520, 541, 592, 665
Sau96I GGNCC 4 cut(s) 9, 136, 385, 560
SduI GDGCHC 4 cut(s) 24, 122, 158, 291
SetI ASST 6 cut(s) 24, 60, 197, 449, 492, 513
SfaNI GCATC 2 cut(s) 431, 507
SfoI GGCGCC 1 cut(s) 315
Sfr274I CTCGAG 1 cut(s) 391
SgrAI CRCCGGYG 1 cut(s) 310
SinI GGWCC 3 cut(s) 9, 385, 560
SlaI CTCGAG 1 cut(s) 391
SmlI CTYRAG 1 cut(s) 391
SmoI CTYRAG 1 cut(s) 391
Sse9I AATT 2 cut(s) 60, 125
SspDI GGCGCC 1 cut(s) 313
SspMI CTAG 1 cut(s) 461
SstI GAGCTC 1 cut(s) 24
StyI CCWWGG 1 cut(s) 596
TaaI ACNGT 3 cut(s) 528, 557, 623
TaqI TCGA 4 cut(s) 88, 205, 281, 392
TaqII GACCGA 1 cut(s) 326
TasI AATT 2 cut(s) 60, 125
TauI GCSGC 6 cut(s) 37, 179, 182, 325, 368, 689
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 2 cut(s) 201, 578
Tru9I TTAA 2 cut(s) 201, 578
TseI GCWGC 2 cut(s) 182, 325
VpaK11BI GGWCC 3 cut(s) 9, 385, 560
XhoI CTCGAG 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 618
XspI CTAG 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.