LRR receptor-like serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 1635, 1967, 2933 |
| AatII | GACGTC | 1 cut(s) | 2685 |
| Acc65I | GGTACC | 1 cut(s) | 353 |
| AccB1I | GGYRCC | 1 cut(s) | 353 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2231 |
| AccI | GTMKAC | 3 cut(s) | 1438, 1767, 2685 |
| AccII | CGCG | 1 cut(s) | 2335 |
| AccIII | TCCGGA | 1 cut(s) | 793 |
| AciI | CCGC | 1 cut(s) | 2492 |
| AclWI | GGATC | 3 cut(s) | 480, 1360, 1462 |
| AcoI | YGGCCR | 1 cut(s) | 2391 |
| AcuI | CTGAAG | 4 cut(s) | 120, 960, 1176, 1592 |
| AcyI | GRCGYC | 1 cut(s) | 2682 |
| AfaI | GTAC | 5 cut(s) | 280, 355, 1934, 2360, 2729 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 414, 793, 926, 1307, 1955, 2231, 2368 |
| AflIII | ACRYGT | 2 cut(s) | 618, 1566 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 148, 814 |
| AhlI | ACTAGT | 2 cut(s) | 194, 388 |
| AjnI | CCWGG | 2 cut(s) | 1729, 1842 |
| AjuI | GAANNNNNNNTTGG | 1 cut(s) | 2970 |
| AleI | CACNNNNGTG | 1 cut(s) | 56 |
| Alw21I | GWGCWC | 1 cut(s) | 1963 |
| Alw26I | GTCTC | 3 cut(s) | 801, 822, 2502 |
| AlwI | GGATC | 3 cut(s) | 480, 1360, 1462 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 434 |
| Aor13HI | TCCGGA | 1 cut(s) | 793 |
| AoxI | GGCC | 5 cut(s) | 784, 1072, 1901, 2391, 2903 |
| ApeKI | GCWGC | 2 cut(s) | 1879, 2182 |
| Asp718I | GGTACC | 1 cut(s) | 353 |
| AspLEI | GCGC | 1 cut(s) | 115 |
| AspS9I | GGNCC | 4 cut(s) | 785, 857, 1793, 2607 |
| AsuHPI | GGTGA | 5 cut(s) | 1699, 2246, 2446, 2677, 2754 |
| AsuNHI | GCTAGC | 1 cut(s) | 2866 |
| AvaII | GGWCC | 3 cut(s) | 857, 1793, 2607 |
| BalI | TGGCCA | 1 cut(s) | 2393 |
| BanI | GGYRCC | 1 cut(s) | 353 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2199, 2231 |
| BbsI | GAAGAC | 1 cut(s) | 1164 |
| Bbv12I | GWGCWC | 1 cut(s) | 1963 |
| BbvI | GCAGC | 2 cut(s) | 1891, 2169 |
| BccI | CCATC | 8 cut(s) | 153, 581, 586, 1121, 2225, 2301, 2801, 2864 |
| BciT130I | CCWGG | 2 cut(s) | 1731, 1844 |
| BclI | TGATCA | 1 cut(s) | 243 |
| BcoDI | GTCTC | 3 cut(s) | 801, 822, 2502 |
| BcuI | ACTAGT | 2 cut(s) | 194, 388 |
| BfaI | CTAG | 9 cut(s) | 195, 389, 653, 1890, 2409, 2534, 2867, 2907, 2939 |
| BfmI | CTRYAG | 3 cut(s) | 1869, 1877, 2686 |
| BisI | GCNGC | 2 cut(s) | 1880, 2183 |
| BlpI | GCTNAGC | 1 cut(s) | 2577 |
| BlsI | GCNGC | 2 cut(s) | 1881, 2184 |
| Bme1390I | CCNGG | 2 cut(s) | 1731, 1844 |
| Bme18I | GGWCC | 3 cut(s) | 857, 1793, 2607 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 148, 814 |
| BmgT120I | GGNCC | 4 cut(s) | 785, 857, 1793, 2607 |
| BmiI | GGNNCC | 8 cut(s) | 355, 521, 571, 643, 931, 1291, 1794, 1827 |
| BmrFI | CCNGG | 2 cut(s) | 1731, 1844 |
| BmrI | ACTGGG | 1 cut(s) | 716 |
| BmsI | GCATC | 1 cut(s) | 1546 |
| BmtI | GCTAGC | 1 cut(s) | 2870 |
| BmuI | ACTGGG | 1 cut(s) | 716 |
| BpiI | GAAGAC | 1 cut(s) | 1164 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2579, 2611 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1302 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2577 |
| BpuEI | CTTGAG | 5 cut(s) | 338, 1111, 1428, 1490, 2606 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1131 |
| BsaHI | GRCGYC | 1 cut(s) | 2682 |
| BsaJI | CCNNGG | 2 cut(s) | 1522, 1843 |
| BsaWI | WCCGGW | 3 cut(s) | 266, 793, 2791 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 414, 793, 926, 1307, 1955, 2231, 2368 |
| Bse118I | RCCGGY | 1 cut(s) | 107 |
| Bse1I | ACTGG | 5 cut(s) | 722, 1003, 1147, 1291, 2609 |
| Bse3DI | GCAATG | 1 cut(s) | 128 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1131 |
| BseAI | TCCGGA | 1 cut(s) | 793 |
| BseBI | CCWGG | 2 cut(s) | 1731, 1844 |
| BseDI | CCNNGG | 2 cut(s) | 1522, 1843 |
| BseGI | GGATG | 4 cut(s) | 573, 1651, 2812, 2848 |
| BseJI | GATNNNNATC | 1 cut(s) | 1131 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 414, 793, 926, 1307, 1955, 2231, 2368 |
| BseMI | GCAATG | 1 cut(s) | 128 |
| BseNI | ACTGG | 5 cut(s) | 722, 1003, 1147, 1291, 2609 |
| BseRI | GAGGAG | 2 cut(s) | 2254, 2417 |
| BseXI | GCAGC | 2 cut(s) | 1891, 2169 |
| BsgI | GTGCAG | 3 cut(s) | 161, 1084, 1526 |
| Bsh1236I | CGCG | 1 cut(s) | 2335 |
| BshFI | GGCC | 5 cut(s) | 786, 1074, 1903, 2393, 2905 |
| BshNI | GGYRCC | 1 cut(s) | 353 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1963 |
| BsiSI | CCGG | 4 cut(s) | 108, 267, 794, 2792 |
| BslFI | GGGAC | 1 cut(s) | 2473 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 414, 793, 926, 1307, 1955, 2231, 2368 |
| BsmAI | GTCTC | 3 cut(s) | 801, 822, 2502 |
| BsmFI | GGGAC | 1 cut(s) | 2473 |
| BsmI | GAATGC | 2 cut(s) | 1420, 1838 |
| BsnI | GGCC | 5 cut(s) | 786, 1074, 1903, 2393, 2905 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1963 |
| Bsp13I | TCCGGA | 1 cut(s) | 793 |
| Bsp143I | GATC | 6 cut(s) | 243, 328, 472, 1365, 1454, 1465 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2577 |
| Bsp68I | TCGCGA | 1 cut(s) | 2335 |
| BspACI | CCGC | 1 cut(s) | 2492 |
| BspANI | GGCC | 5 cut(s) | 786, 1074, 1903, 2393, 2905 |
| BspEI | TCCGGA | 1 cut(s) | 793 |
| BspFNI | CGCG | 1 cut(s) | 2335 |
| BspHI | TCATGA | 1 cut(s) | 957 |
| BspLI | GGNNCC | 8 cut(s) | 355, 521, 571, 643, 931, 1291, 1794, 1827 |
| BspMAI | CTGCAG | 1 cut(s) | 1881 |
| BspOI | GCTAGC | 1 cut(s) | 2870 |
| BspPI | GGATC | 3 cut(s) | 480, 1360, 1462 |
| BspT107I | GGYRCC | 1 cut(s) | 353 |
| BsrDI | GCAATG | 1 cut(s) | 128 |
| BsrFI | RCCGGY | 1 cut(s) | 107 |
| BsrI | ACTGG | 5 cut(s) | 722, 1003, 1147, 1291, 2609 |
| BssAI | RCCGGY | 1 cut(s) | 107 |
| BssECI | CCNNGG | 2 cut(s) | 1522, 1843 |
| BssMI | GATC | 6 cut(s) | 243, 328, 472, 1365, 1454, 1465 |
| BssNAI | GTATAC | 1 cut(s) | 1439 |
| BssNI | GRCGYC | 1 cut(s) | 2682 |
| BssT1I | CCWWGG | 1 cut(s) | 1522 |
| Bst1107I | GTATAC | 1 cut(s) | 1439 |
| Bst2UI | CCWGG | 2 cut(s) | 1731, 1844 |
| Bst4CI | ACNGT | 5 cut(s) | 425, 924, 2222, 2614, 2727 |
| Bst6I | CTCTTC | 4 cut(s) | 187, 825, 1644, 2143 |
| BstACI | GRCGYC | 1 cut(s) | 2682 |
| BstC8I | GCNNGC | 7 cut(s) | 636, 640, 1505, 2554, 2620, 2868, 2912 |
| BstF5I | GGATG | 4 cut(s) | 573, 1651, 2812, 2848 |
| BstFNI | CGCG | 1 cut(s) | 2335 |
| BstHHI | GCGC | 1 cut(s) | 115 |
| BstKTI | GATC | 6 cut(s) | 246, 331, 475, 1368, 1457, 1468 |
| BstMAI | GTCTC | 3 cut(s) | 801, 822, 2502 |
| BstMBI | GATC | 6 cut(s) | 243, 328, 472, 1365, 1454, 1465 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 104, 1393, 1402, 1618, 2587, 2781, 2911 |
| BstNI | CCWGG | 2 cut(s) | 1731, 1844 |
| BstNSI | RCATGY | 6 cut(s) | 622, 1570, 2041, 2476, 2888, 2914 |
| BstSCI | CCNGG | 2 cut(s) | 1729, 1842 |
| BstSFI | CTRYAG | 3 cut(s) | 1869, 1877, 2686 |
| BstUI | CGCG | 1 cut(s) | 2335 |
| BstV1I | GCAGC | 2 cut(s) | 1891, 2169 |
| BstV2I | GAAGAC | 1 cut(s) | 1164 |
| BstX2I | RGATCY | 2 cut(s) | 472, 1365 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 207 |
| BstYI | RGATCY | 2 cut(s) | 472, 1365 |
| BstZ17I | GTATAC | 1 cut(s) | 1439 |
| BsuRI | GGCC | 5 cut(s) | 786, 1074, 1903, 2393, 2905 |
| BtsCI | GGATG | 4 cut(s) | 573, 1651, 2812, 2848 |
| BtsI | GCAGTG | 3 cut(s) | 228, 1047, 2571 |
| BtsIMutI | CAGTG | 7 cut(s) | 228, 859, 920, 1047, 1140, 1284, 2571 |
| BtuMI | TCGCGA | 1 cut(s) | 2335 |
| Cac8I | GCNNGC | 7 cut(s) | 636, 640, 1505, 2554, 2620, 2868, 2912 |
| CaiI | CAGNNNCTG | 1 cut(s) | 434 |
| CciI | TCATGA | 1 cut(s) | 957 |
| CfoI | GCGC | 1 cut(s) | 115 |
| Cfr10I | RCCGGY | 1 cut(s) | 107 |
| Cfr13I | GGNCC | 4 cut(s) | 785, 857, 1793, 2607 |
| CseI | GACGC | 2 cut(s) | 2546, 2927 |
| Csp6I | GTAC | 5 cut(s) | 279, 354, 1933, 2359, 2728 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 516, 551, 1732, 1767 |
| CviQI | GTAC | 5 cut(s) | 279, 354, 1933, 2359, 2728 |
| DpnI | GATC | 6 cut(s) | 245, 330, 474, 1367, 1456, 1467 |
| DpnII | GATC | 6 cut(s) | 243, 328, 472, 1365, 1454, 1465 |
| DriI | GACNNNNNGTC | 2 cut(s) | 148, 814 |
| EaeI | YGGCCR | 1 cut(s) | 2391 |
| Eam1104I | CTCTTC | 4 cut(s) | 187, 825, 1644, 2143 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 148, 814 |
| EarI | CTCTTC | 4 cut(s) | 187, 825, 1644, 2143 |
| Eco130I | CCWWGG | 1 cut(s) | 1522 |
| Eco147I | AGGCCT | 2 cut(s) | 1903, 2905 |
| Eco47I | GGWCC | 3 cut(s) | 857, 1793, 2607 |
| Eco57I | CTGAAG | 4 cut(s) | 120, 960, 1176, 1592 |
| EcoO109I | RGGNCCY | 1 cut(s) | 785 |
| EcoRI | GAATTC | 1 cut(s) | 2942 |
| EcoRII | CCWGG | 2 cut(s) | 1729, 1842 |
| EcoT14I | CCWWGG | 1 cut(s) | 1522 |
| EcoT22I | ATGCAT | 1 cut(s) | 1539 |
| ErhI | CCWWGG | 1 cut(s) | 1522 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1375, 1407, 2243, 2275 |
| FaqI | GGGAC | 1 cut(s) | 2473 |
| FauI | CCCGC | 1 cut(s) | 2499 |
| FbaI | TGATCA | 1 cut(s) | 243 |
| FblI | GTMKAC | 3 cut(s) | 1438, 1767, 2685 |
| Fnu4HI | GCNGC | 2 cut(s) | 1880, 2183 |
| FokI | GGATG | 4 cut(s) | 560, 1658, 2819, 2855 |
| Fsp4HI | GCNGC | 2 cut(s) | 1880, 2183 |
| FspBI | CTAG | 9 cut(s) | 195, 389, 653, 1890, 2409, 2534, 2867, 2907, 2939 |
| GlaI | GCGC | 1 cut(s) | 114 |
| GluI | GCNGC | 2 cut(s) | 1880, 2183 |
| HaeIII | GGCC | 5 cut(s) | 786, 1074, 1903, 2393, 2905 |
| HapII | CCGG | 4 cut(s) | 108, 267, 794, 2792 |
| HgaI | GACGC | 2 cut(s) | 2546, 2927 |
| HhaI | GCGC | 1 cut(s) | 115 |
| Hin1I | GRCGYC | 1 cut(s) | 2682 |
| Hin6I | GCGC | 1 cut(s) | 113 |
| HinP1I | GCGC | 1 cut(s) | 113 |
| HincII | GTYRAC | 2 cut(s) | 1768, 2018 |
| HindII | GTYRAC | 2 cut(s) | 1768, 2018 |
| HindIII | AAGCTT | 5 cut(s) | 1034, 1087, 1403, 2309, 2579 |
| HinfI | GANTC | 5 cut(s) | 313, 1116, 1318, 2132, 2594 |
| HpaII | CCGG | 4 cut(s) | 108, 267, 794, 2792 |
| HphI | GGTGA | 5 cut(s) | 1699, 2246, 2446, 2677, 2754 |
| Hpy99I | CGWCG | 2 cut(s) | 307, 1769 |
| HpyAV | CCTTC | 6 cut(s) | 146, 701, 1377, 2656, 2774, 2825 |
| HpyCH4III | ACNGT | 5 cut(s) | 425, 924, 2222, 2614, 2727 |
| HpyCH4IV | ACGT | 5 cut(s) | 53, 87, 630, 2014, 2682 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 104, 1393, 1402, 1618, 2587, 2781, 2911 |
| HpySE526I | ACGT | 5 cut(s) | 53, 87, 630, 2014, 2682 |
| Hsp92I | GRCGYC | 1 cut(s) | 2682 |
| HspAI | GCGC | 1 cut(s) | 113 |
| Kpn2I | TCCGGA | 1 cut(s) | 793 |
| KpnI | GGTACC | 1 cut(s) | 357 |
| Ksp22I | TGATCA | 1 cut(s) | 243 |
| Kzo9I | GATC | 6 cut(s) | 243, 328, 472, 1365, 1454, 1465 |
| LmnI | GCTCC | 5 cut(s) | 538, 647, 1831, 1958, 2241 |
| Lsp1109I | GCAGC | 2 cut(s) | 1891, 2169 |
| LweI | GCATC | 1 cut(s) | 1546 |
| MaeI | CTAG | 9 cut(s) | 195, 389, 653, 1890, 2409, 2534, 2867, 2907, 2939 |
| MaeII | ACGT | 5 cut(s) | 53, 87, 630, 2014, 2682 |
| MalI | GATC | 6 cut(s) | 245, 330, 474, 1367, 1456, 1467 |
| MboI | GATC | 6 cut(s) | 243, 328, 472, 1365, 1454, 1465 |
| MfeI | CAATTG | 1 cut(s) | 2496 |
| MflI | RGATCY | 2 cut(s) | 472, 1365 |
| MhlI | GDGCHC | 1 cut(s) | 1963 |
| MlsI | TGGCCA | 1 cut(s) | 2393 |
| MluNI | TGGCCA | 1 cut(s) | 2393 |
| MlyI | GAGTC | 3 cut(s) | 1125, 1312, 2588 |
| MmeI | TCCRAC | 3 cut(s) | 210, 1144, 2140 |
| Mox20I | TGGCCA | 1 cut(s) | 2393 |
| Mph1103I | ATGCAT | 1 cut(s) | 1539 |
| MroI | TCCGGA | 1 cut(s) | 793 |
| MscI | TGGCCA | 1 cut(s) | 2393 |
| MseI | TTAA | 9 cut(s) | 596, 605, 995, 1335, 2261, 2279, 2445, 2718, 2771 |
| MslI | CAYNNNNRTG | 3 cut(s) | 56, 233, 2481 |
| Msp20I | TGGCCA | 1 cut(s) | 2393 |
| MspI | CCGG | 4 cut(s) | 108, 267, 794, 2792 |
| MspR9I | CCNGG | 2 cut(s) | 1731, 1844 |
| MunI | CAATTG | 1 cut(s) | 2496 |
| Mva1269I | GAATGC | 2 cut(s) | 1420, 1838 |
| MvaI | CCWGG | 2 cut(s) | 1731, 1844 |
| MvnI | CGCG | 1 cut(s) | 2335 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 104, 1393, 1402, 1618, 2587, 2781, 2911 |
| NdeII | GATC | 6 cut(s) | 243, 328, 472, 1365, 1454, 1465 |
| NheI | GCTAGC | 1 cut(s) | 2866 |
| NlaIV | GGNNCC | 8 cut(s) | 355, 521, 571, 643, 931, 1291, 1794, 1827 |
| NmuCI | GTSAC | 8 cut(s) | 150, 734, 911, 1687, 2033, 2665, 2678, 2760 |
| NruI | TCGCGA | 1 cut(s) | 2335 |
| NsiI | ATGCAT | 1 cut(s) | 1539 |
| NspI | RCATGY | 6 cut(s) | 622, 1570, 2041, 2476, 2888, 2914 |
| OliI | CACNNNNGTG | 1 cut(s) | 56 |
| PaeI | GCATGC | 1 cut(s) | 2914 |
| PagI | TCATGA | 1 cut(s) | 957 |
| PceI | AGGCCT | 2 cut(s) | 1903, 2905 |
| PciI | ACATGT | 2 cut(s) | 618, 1566 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 302, 308 |
| PctI | GAATGC | 2 cut(s) | 1420, 1838 |
| PfeI | GAWTC | 2 cut(s) | 313, 2132 |
| PflFI | GACNNNGTC | 1 cut(s) | 262 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2231 |
| PkrI | GCNGC | 2 cut(s) | 1881, 2184 |
| PleI | GAGTC | 3 cut(s) | 1124, 1312, 2588 |
| PpsI | GAGTC | 3 cut(s) | 1124, 1312, 2588 |
| PscI | ACATGT | 2 cut(s) | 618, 1566 |
| PsiI | TTATAA | 3 cut(s) | 1635, 1967, 2933 |
| Psp6I | CCWGG | 2 cut(s) | 1729, 1842 |
| PspGI | CCWGG | 2 cut(s) | 1729, 1842 |
| PspN4I | GGNNCC | 8 cut(s) | 355, 521, 571, 643, 931, 1291, 1794, 1827 |
| PspPI | GGNCC | 4 cut(s) | 785, 857, 1793, 2607 |
| PstI | CTGCAG | 1 cut(s) | 1881 |
| PstNI | CAGNNNCTG | 1 cut(s) | 434 |
| PsuI | RGATCY | 2 cut(s) | 472, 1365 |
| PsyI | GACNNNGTC | 1 cut(s) | 262 |
| RruI | TCGCGA | 1 cut(s) | 2335 |
| RsaI | GTAC | 5 cut(s) | 280, 355, 1934, 2360, 2729 |
| RsaNI | GTAC | 5 cut(s) | 279, 354, 1933, 2359, 2728 |
| RseI | CAYNNNNRTG | 3 cut(s) | 56, 233, 2481 |
| SalI | GTCGAC | 1 cut(s) | 1766 |
| SaqAI | TTAA | 9 cut(s) | 596, 605, 995, 1335, 2261, 2279, 2445, 2718, 2771 |
| SatI | GCNGC | 2 cut(s) | 1880, 2183 |
| Sau3AI | GATC | 6 cut(s) | 243, 328, 472, 1365, 1454, 1465 |
| Sau96I | GGNCC | 4 cut(s) | 785, 857, 1793, 2607 |
| SchI | GAGTC | 3 cut(s) | 1125, 1312, 2588 |
| ScrFI | CCNGG | 2 cut(s) | 1731, 1844 |
| SduI | GDGCHC | 1 cut(s) | 1963 |
| SfaNI | GCATC | 1 cut(s) | 1546 |
| SfcI | CTRYAG | 3 cut(s) | 1869, 1877, 2686 |
| SinI | GGWCC | 3 cut(s) | 857, 1793, 2607 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 56, 233, 2481 |
| SmlI | CTYRAG | 5 cut(s) | 317, 1090, 1443, 1469, 2585 |
| SmoI | CTYRAG | 5 cut(s) | 317, 1090, 1443, 1469, 2585 |
| SpeI | ACTAGT | 2 cut(s) | 194, 388 |
| SphI | GCATGC | 1 cut(s) | 2914 |
| SseBI | AGGCCT | 2 cut(s) | 1903, 2905 |
| SsiI | CCGC | 1 cut(s) | 2492 |
| SspI | AATATT | 2 cut(s) | 802, 2740 |
| SspMI | CTAG | 9 cut(s) | 195, 389, 653, 1890, 2409, 2534, 2867, 2907, 2939 |
| StuI | AGGCCT | 2 cut(s) | 1903, 2905 |
| StyD4I | CCNGG | 2 cut(s) | 1729, 1842 |
| StyI | CCWWGG | 1 cut(s) | 1522 |
| TaaI | ACNGT | 5 cut(s) | 425, 924, 2222, 2614, 2727 |
| TaiI | ACGT | 5 cut(s) | 56, 90, 633, 2017, 2685 |
| TaqI | TCGA | 5 cut(s) | 1767, 1910, 2072, 2296, 2506 |
| TaqII | GACCGA | 1 cut(s) | 138 |
| TatI | WGTACW | 1 cut(s) | 1932 |
| TfiI | GAWTC | 2 cut(s) | 313, 2132 |
| Tru1I | TTAA | 9 cut(s) | 596, 605, 995, 1335, 2261, 2279, 2445, 2718, 2771 |
| Tru9I | TTAA | 9 cut(s) | 596, 605, 995, 1335, 2261, 2279, 2445, 2718, 2771 |
| TscAI | CASTG | 7 cut(s) | 235, 859, 927, 1054, 1147, 1291, 2578 |
| TseFI | GTSAC | 8 cut(s) | 150, 734, 911, 1687, 2033, 2665, 2678, 2760 |
| TseI | GCWGC | 2 cut(s) | 1879, 2182 |
| Tsp45I | GTSAC | 8 cut(s) | 150, 734, 911, 1687, 2033, 2665, 2678, 2760 |
| TspRI | CASTG | 7 cut(s) | 235, 859, 927, 1054, 1147, 1291, 2578 |
| Tth111I | GACNNNGTC | 1 cut(s) | 262 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2231 |
| VpaK11BI | GGWCC | 3 cut(s) | 857, 1793, 2607 |
| XceI | RCATGY | 6 cut(s) | 622, 1570, 2041, 2476, 2888, 2914 |
| XmiI | GTMKAC | 3 cut(s) | 1438, 1767, 2685 |
| XspI | CTAG | 9 cut(s) | 195, 389, 653, 1890, 2409, 2534, 2867, 2907, 2939 |
| ZraI | GACGTC | 1 cut(s) | 2683 |
| Zsp2I | ATGCAT | 1 cut(s) | 1539 |