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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 483 |
| Acc65I | GGTACC | 1 cut(s) | 293 |
| AccB1I | GGYRCC | 2 cut(s) | 293, 674 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 583 |
| AccBSI | CCGCTC | 1 cut(s) | 306 |
| AccII | CGCG | 2 cut(s) | 338, 453 |
| AccIII | TCCGGA | 1 cut(s) | 663 |
| AciI | CCGC | 8 cut(s) | 90, 268, 271, 291, 304, 338, 387, 672 |
| AclWI | GGATC | 4 cut(s) | 213, 214, 655, 668 |
| AcoI | YGGCCR | 2 cut(s) | 107, 301 |
| AcsI | RAATTY | 2 cut(s) | 712, 785 |
| AfaI | GTAC | 2 cut(s) | 295, 581 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 280, 350, 409, 583 |
| AgsI | TTSAA | 3 cut(s) | 556, 703, 783 |
| AjiI | CACGTC | 1 cut(s) | 382 |
| AluBI | AGCT | 3 cut(s) | 362, 422, 448 |
| AluI | AGCT | 3 cut(s) | 362, 422, 448 |
| Alw21I | GWGCWC | 1 cut(s) | 710 |
| Alw26I | GTCTC | 1 cut(s) | 727 |
| AlwI | GGATC | 4 cut(s) | 213, 214, 655, 668 |
| Aor13HI | TCCGGA | 1 cut(s) | 663 |
| AoxI | GGCC | 3 cut(s) | 107, 301, 472 |
| ApeKI | GCWGC | 1 cut(s) | 448 |
| ApoI | RAATTY | 2 cut(s) | 712, 785 |
| Asp718I | GGTACC | 1 cut(s) | 293 |
| AspLEI | GCGC | 3 cut(s) | 266, 453, 455 |
| AspS9I | GGNCC | 3 cut(s) | 8, 341, 473 |
| AsuC2I | CCSGG | 2 cut(s) | 6, 70 |
| AsuII | TTCGAA | 1 cut(s) | 189 |
| AsuNHI | GCTAGC | 1 cut(s) | 418 |
| AvaII | GGWCC | 2 cut(s) | 8, 341 |
| BamHI | GGATCC | 1 cut(s) | 660 |
| BanI | GGYRCC | 2 cut(s) | 293, 674 |
| BanII | GRGCYC | 1 cut(s) | 233 |
| BauI | CACGAG | 1 cut(s) | 94 |
| Bbv12I | GWGCWC | 1 cut(s) | 710 |
| BbvI | GCAGC | 1 cut(s) | 435 |
| BccI | CCATC | 1 cut(s) | 121 |
| BceAI | ACGGC | 2 cut(s) | 87, 685 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 183, 217 |
| BcnI | CCSGG | 2 cut(s) | 6, 70 |
| BcoDI | GTCTC | 1 cut(s) | 727 |
| BfaI | CTAG | 2 cut(s) | 84, 419 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 683 |
| BglII | AGATCT | 2 cut(s) | 535, 776 |
| BisI | GCNGC | 4 cut(s) | 271, 304, 387, 449 |
| BlsI | GCNGC | 4 cut(s) | 272, 305, 388, 450 |
| BmcAI | AGTACT | 1 cut(s) | 581 |
| Bme1390I | CCNGG | 2 cut(s) | 6, 70 |
| Bme18I | GGWCC | 2 cut(s) | 8, 341 |
| BmgBI | CACGTC | 1 cut(s) | 382 |
| BmgT120I | GGNCC | 3 cut(s) | 8, 341, 473 |
| BmiI | GGNNCC | 6 cut(s) | 10, 295, 342, 475, 662, 676 |
| BmrFI | CCNGG | 2 cut(s) | 6, 70 |
| BmrI | ACTGGG | 1 cut(s) | 571 |
| BmsI | GCATC | 1 cut(s) | 585 |
| BmtI | GCTAGC | 1 cut(s) | 422 |
| BmuI | ACTGGG | 1 cut(s) | 571 |
| Bpu10I | CCTNAGC | 2 cut(s) | 28, 444 |
| Bpu14I | TTCGAA | 1 cut(s) | 189 |
| BpuMI | CCSGG | 2 cut(s) | 6, 70 |
| BsaJI | CCNNGG | 1 cut(s) | 68 |
| BsaWI | WCCGGW | 1 cut(s) | 663 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 46, 76 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 280, 350, 409, 583 |
| Bse118I | RCCGGY | 1 cut(s) | 299 |
| Bse1I | ACTGG | 1 cut(s) | 577 |
| BseAI | TCCGGA | 1 cut(s) | 663 |
| BseDI | CCNNGG | 1 cut(s) | 68 |
| BseGI | GGATG | 1 cut(s) | 113 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 280, 350, 409, 583 |
| BseNI | ACTGG | 1 cut(s) | 577 |
| BsePI | GCGCGC | 1 cut(s) | 451 |
| BseRI | GAGGAG | 1 cut(s) | 111 |
| BseX3I | CGGCCG | 1 cut(s) | 301 |
| BseXI | GCAGC | 1 cut(s) | 435 |
| Bsh1236I | CGCG | 2 cut(s) | 338, 453 |
| Bsh1285I | CGRYCG | 2 cut(s) | 304, 329 |
| BshFI | GGCC | 3 cut(s) | 109, 303, 474 |
| BshNI | GGYRCC | 2 cut(s) | 293, 674 |
| BsiEI | CGRYCG | 2 cut(s) | 304, 329 |
| BsiHKAI | GWGCWC | 1 cut(s) | 710 |
| BsiSI | CCGG | 4 cut(s) | 6, 70, 300, 664 |
| BslFI | GGGAC | 3 cut(s) | 296, 354, 544 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 280, 350, 409, 583 |
| BsmAI | GTCTC | 1 cut(s) | 727 |
| BsmFI | GGGAC | 3 cut(s) | 296, 354, 544 |
| BsmI | GAATGC | 1 cut(s) | 293 |
| BsnI | GGCC | 3 cut(s) | 109, 303, 474 |
| Bsp119I | TTCGAA | 1 cut(s) | 189 |
| Bsp1286I | GDGCHC | 2 cut(s) | 233, 710 |
| Bsp13I | TCCGGA | 1 cut(s) | 663 |
| Bsp143I | GATC | 7 cut(s) | 205, 219, 275, 326, 535, 660, 776 |
| BspACI | CCGC | 8 cut(s) | 90, 268, 271, 291, 304, 338, 387, 672 |
| BspANI | GGCC | 3 cut(s) | 109, 303, 474 |
| BspEI | TCCGGA | 1 cut(s) | 663 |
| BspFNI | CGCG | 2 cut(s) | 338, 453 |
| BspLI | GGNNCC | 6 cut(s) | 10, 295, 342, 475, 662, 676 |
| BspOI | GCTAGC | 1 cut(s) | 422 |
| BspPI | GGATC | 4 cut(s) | 213, 214, 655, 668 |
| BspT104I | TTCGAA | 1 cut(s) | 189 |
| BspT107I | GGYRCC | 2 cut(s) | 293, 674 |
| BsrBI | CCGCTC | 1 cut(s) | 306 |
| BsrFI | RCCGGY | 1 cut(s) | 299 |
| BsrI | ACTGG | 1 cut(s) | 577 |
| BssAI | RCCGGY | 1 cut(s) | 299 |
| BssECI | CCNNGG | 1 cut(s) | 68 |
| BssHII | GCGCGC | 1 cut(s) | 451 |
| BssMI | GATC | 7 cut(s) | 205, 219, 275, 326, 535, 660, 776 |
| BssSI | CACGAG | 1 cut(s) | 94 |
| Bst2BI | CACGAG | 1 cut(s) | 94 |
| Bst4CI | ACNGT | 3 cut(s) | 432, 506, 756 |
| BstBI | TTCGAA | 1 cut(s) | 189 |
| BstC8I | GCNNGC | 6 cut(s) | 268, 356, 360, 420, 453, 472 |
| BstDEI | CTNAG | 2 cut(s) | 28, 444 |
| BstF5I | GGATG | 1 cut(s) | 113 |
| BstFNI | CGCG | 2 cut(s) | 338, 453 |
| BstHHI | GCGC | 3 cut(s) | 266, 453, 455 |
| BstKTI | GATC | 7 cut(s) | 208, 222, 278, 329, 538, 663, 779 |
| BstMAI | GTCTC | 1 cut(s) | 727 |
| BstMBI | GATC | 7 cut(s) | 205, 219, 275, 326, 535, 660, 776 |
| BstMCI | CGRYCG | 2 cut(s) | 304, 329 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 270, 395, 419, 683 |
| BstSCI | CCNGG | 2 cut(s) | 4, 68 |
| BstUI | CGCG | 2 cut(s) | 338, 453 |
| BstV1I | GCAGC | 1 cut(s) | 435 |
| BstX2I | RGATCY | 3 cut(s) | 535, 660, 776 |
| BstYI | RGATCY | 3 cut(s) | 535, 660, 776 |
| BstZI | CGGCCG | 1 cut(s) | 301 |
| BsuRI | GGCC | 3 cut(s) | 109, 303, 474 |
| BtrI | CACGTC | 1 cut(s) | 382 |
| BtsCI | GGATG | 1 cut(s) | 113 |
| BtsIMutI | CAGTG | 1 cut(s) | 428 |
| Cac8I | GCNNGC | 6 cut(s) | 268, 356, 360, 420, 453, 472 |
| CfoI | GCGC | 3 cut(s) | 266, 453, 455 |
| Cfr10I | RCCGGY | 1 cut(s) | 299 |
| Cfr13I | GGNCC | 3 cut(s) | 8, 341, 473 |
| CseI | GACGC | 1 cut(s) | 344 |
| Csp6I | GTAC | 2 cut(s) | 294, 580 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 336, 371 |
| CviAII | CATG | 2 cut(s) | 183, 467 |
| CviQI | GTAC | 2 cut(s) | 294, 580 |
| DdeI | CTNAG | 2 cut(s) | 28, 444 |
| DpnI | GATC | 7 cut(s) | 207, 221, 277, 328, 537, 662, 778 |
| DpnII | GATC | 7 cut(s) | 205, 219, 275, 326, 535, 660, 776 |
| EaeI | YGGCCR | 2 cut(s) | 107, 301 |
| EagI | CGGCCG | 1 cut(s) | 301 |
| EclXI | CGGCCG | 1 cut(s) | 301 |
| Eco24I | GRGCYC | 1 cut(s) | 233 |
| Eco32I | GATATC | 1 cut(s) | 694 |
| Eco47I | GGWCC | 2 cut(s) | 8, 341 |
| Eco52I | CGGCCG | 1 cut(s) | 301 |
| EcoO109I | RGGNCCY | 1 cut(s) | 473 |
| EcoRV | GATATC | 1 cut(s) | 694 |
| EcoT38I | GRGCYC | 1 cut(s) | 233 |
| FaeI | CATG | 2 cut(s) | 186, 470 |
| FaqI | GGGAC | 3 cut(s) | 296, 354, 544 |
| FatI | CATG | 2 cut(s) | 182, 466 |
| FauI | CCCGC | 2 cut(s) | 275, 331 |
| Fnu4HI | GCNGC | 4 cut(s) | 271, 304, 387, 449 |
| FokI | GGATG | 1 cut(s) | 100 |
| FriOI | GRGCYC | 1 cut(s) | 233 |
| Fsp4HI | GCNGC | 4 cut(s) | 271, 304, 387, 449 |
| FspBI | CTAG | 2 cut(s) | 84, 419 |
| GlaI | GCGC | 3 cut(s) | 265, 452, 454 |
| GluI | GCNGC | 4 cut(s) | 271, 304, 387, 449 |
| HaeIII | GGCC | 3 cut(s) | 109, 303, 474 |
| HapII | CCGG | 4 cut(s) | 6, 70, 300, 664 |
| HgaI | GACGC | 1 cut(s) | 344 |
| HhaI | GCGC | 3 cut(s) | 266, 453, 455 |
| Hin1II | CATG | 2 cut(s) | 186, 470 |
| Hin6I | GCGC | 3 cut(s) | 264, 451, 453 |
| HinP1I | GCGC | 3 cut(s) | 264, 451, 453 |
| HincII | GTYRAC | 1 cut(s) | 435 |
| HindII | GTYRAC | 1 cut(s) | 435 |
| HinfI | GANTC | 3 cut(s) | 80, 315, 616 |
| HpaII | CCGG | 4 cut(s) | 6, 70, 300, 664 |
| Hpy166II | GTNNAC | 1 cut(s) | 435 |
| Hpy188I | TCNGA | 2 cut(s) | 205, 210 |
| Hpy188III | TCNNGA | 1 cut(s) | 664 |
| Hpy8I | GTNNAC | 1 cut(s) | 435 |
| Hpy99I | CGWCG | 2 cut(s) | 108, 386 |
| HpyAV | CCTTC | 1 cut(s) | 609 |
| HpyCH4III | ACNGT | 3 cut(s) | 432, 506, 756 |
| HpyCH4IV | ACGT | 1 cut(s) | 381 |
| HpyCH4V | TGCA | 3 cut(s) | 358, 470, 498 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 270, 395, 419, 683 |
| HpyF3I | CTNAG | 2 cut(s) | 28, 444 |
| HpySE526I | ACGT | 1 cut(s) | 381 |
| Hsp92II | CATG | 2 cut(s) | 186, 470 |
| HspAI | GCGC | 3 cut(s) | 264, 451, 453 |
| Kpn2I | TCCGGA | 1 cut(s) | 663 |
| KpnI | GGTACC | 1 cut(s) | 297 |
| Kzo9I | GATC | 7 cut(s) | 205, 219, 275, 326, 535, 660, 776 |
| LmnI | GCTCC | 1 cut(s) | 311 |
| Lsp1109I | GCAGC | 1 cut(s) | 435 |
| LweI | GCATC | 1 cut(s) | 585 |
| MaeI | CTAG | 2 cut(s) | 84, 419 |
| MaeII | ACGT | 1 cut(s) | 381 |
| MaeIII | GTNAC | 2 cut(s) | 72, 608 |
| MalI | GATC | 7 cut(s) | 207, 221, 277, 328, 537, 662, 778 |
| MbiI | CCGCTC | 1 cut(s) | 306 |
| MboI | GATC | 7 cut(s) | 205, 219, 275, 326, 535, 660, 776 |
| MboII | GAAGA | 5 cut(s) | 530, 541, 544, 737, 771 |
| MflI | RGATCY | 3 cut(s) | 535, 660, 776 |
| MhlI | GDGCHC | 2 cut(s) | 233, 710 |
| MluCI | AATT | 4 cut(s) | 478, 712, 767, 785 |
| MlyI | GAGTC | 2 cut(s) | 74, 324 |
| MnlI | CCTC | 8 cut(s) | 72, 132, 168, 257, 268, 580, 634, 662 |
| MroI | TCCGGA | 1 cut(s) | 663 |
| MseI | TTAA | 1 cut(s) | 321 |
| MslI | CAYNNNNRTG | 1 cut(s) | 642 |
| MspI | CCGG | 4 cut(s) | 6, 70, 300, 664 |
| MspR9I | CCNGG | 2 cut(s) | 6, 70 |
| Mva1269I | GAATGC | 1 cut(s) | 293 |
| MvnI | CGCG | 2 cut(s) | 338, 453 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 270, 395, 419, 683 |
| NciI | CCSGG | 2 cut(s) | 6, 70 |
| NdeII | GATC | 7 cut(s) | 205, 219, 275, 326, 535, 660, 776 |
| NheI | GCTAGC | 1 cut(s) | 418 |
| NlaIII | CATG | 2 cut(s) | 186, 470 |
| NlaIV | GGNNCC | 6 cut(s) | 10, 295, 342, 475, 662, 676 |
| NspV | TTCGAA | 1 cut(s) | 189 |
| PauI | GCGCGC | 1 cut(s) | 451 |
| PctI | GAATGC | 1 cut(s) | 293 |
| PfeI | GAWTC | 1 cut(s) | 616 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 583 |
| PkrI | GCNGC | 4 cut(s) | 272, 305, 388, 450 |
| Ple19I | CGATCG | 1 cut(s) | 329 |
| PleI | GAGTC | 2 cut(s) | 74, 323 |
| PpsI | GAGTC | 2 cut(s) | 74, 323 |
| PsiI | TTATAA | 1 cut(s) | 483 |
| PspN4I | GGNNCC | 6 cut(s) | 10, 295, 342, 475, 662, 676 |
| PspPI | GGNCC | 3 cut(s) | 8, 341, 473 |
| PsuI | RGATCY | 3 cut(s) | 535, 660, 776 |
| PteI | GCGCGC | 1 cut(s) | 451 |
| PvuI | CGATCG | 1 cut(s) | 329 |
| RsaI | GTAC | 2 cut(s) | 295, 581 |
| RsaNI | GTAC | 2 cut(s) | 294, 580 |
| RseI | CAYNNNNRTG | 1 cut(s) | 642 |
| SaqAI | TTAA | 1 cut(s) | 321 |
| SatI | GCNGC | 4 cut(s) | 271, 304, 387, 449 |
| Sau3AI | GATC | 7 cut(s) | 205, 219, 275, 326, 535, 660, 776 |
| Sau96I | GGNCC | 3 cut(s) | 8, 341, 473 |
| ScaI | AGTACT | 1 cut(s) | 581 |
| SchI | GAGTC | 2 cut(s) | 74, 324 |
| ScrFI | CCNGG | 2 cut(s) | 6, 70 |
| SduI | GDGCHC | 2 cut(s) | 233, 710 |
| SfaNI | GCATC | 1 cut(s) | 585 |
| SfuI | TTCGAA | 1 cut(s) | 189 |
| SinI | GGWCC | 2 cut(s) | 8, 341 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 642 |
| Sse9I | AATT | 4 cut(s) | 478, 712, 767, 785 |
| SsiI | CCGC | 8 cut(s) | 90, 268, 271, 291, 304, 338, 387, 672 |
| SspMI | CTAG | 2 cut(s) | 84, 419 |
| StyD4I | CCNGG | 2 cut(s) | 4, 68 |
| TaaI | ACNGT | 3 cut(s) | 432, 506, 756 |
| TaiI | ACGT | 1 cut(s) | 384 |
| TaqI | TCGA | 5 cut(s) | 189, 218, 329, 374, 619 |
| TasI | AATT | 4 cut(s) | 478, 712, 767, 785 |
| TatI | WGTACW | 1 cut(s) | 579 |
| TauI | GCSGC | 3 cut(s) | 273, 306, 389 |
| TfiI | GAWTC | 1 cut(s) | 616 |
| Tru1I | TTAA | 1 cut(s) | 321 |
| Tru9I | TTAA | 1 cut(s) | 321 |
| TscAI | CASTG | 1 cut(s) | 435 |
| TseI | GCWGC | 1 cut(s) | 448 |
| TspDTI | ATGAA | 1 cut(s) | 530 |
| TspRI | CASTG | 1 cut(s) | 435 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 583 |
| VpaK11BI | GGWCC | 2 cut(s) | 8, 341 |
| XapI | RAATTY | 2 cut(s) | 712, 785 |
| XspI | CTAG | 2 cut(s) | 84, 419 |
| ZrmI | AGTACT | 1 cut(s) | 581 |