LRR receptor-like serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 914 |
| AarI | CACCTGC | 1 cut(s) | 852 |
| Acc36I | ACCTGC | 3 cut(s) | 184, 852, 2186 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 2017, 2033 |
| AccI | GTMKAC | 4 cut(s) | 753, 996, 1398, 1902 |
| AccIII | TCCGGA | 1 cut(s) | 1066 |
| AciI | CCGC | 4 cut(s) | 1044, 1341, 1783, 1862 |
| AclI | AACGTT | 1 cut(s) | 764 |
| AclWI | GGATC | 5 cut(s) | 892, 1130, 2081, 2137, 2150 |
| AcsI | RAATTY | 6 cut(s) | 443, 516, 587, 599, 1158, 1316 |
| AcuI | CTGAAG | 2 cut(s) | 80, 1116 |
| AdeI | CACNNNGTG | 1 cut(s) | 199 |
| AfaI | GTAC | 5 cut(s) | 205, 851, 1367, 1586, 2077 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 470, 542, 1043, 1187, 1569, 1575, 1848, 2017, 2033 |
| AflIII | ACRYGT | 1 cut(s) | 206 |
| AjiI | CACGTC | 1 cut(s) | 209 |
| AjnI | CCWGG | 4 cut(s) | 1568, 1948, 2089, 2138 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1047, 1079 |
| AleI | CACNNNNGTG | 1 cut(s) | 1676 |
| Alw21I | GWGCWC | 3 cut(s) | 1583, 1740, 1835 |
| Alw26I | GTCTC | 2 cut(s) | 650, 940 |
| AlwI | GGATC | 5 cut(s) | 892, 1130, 2081, 2137, 2150 |
| Ama87I | CYCGRG | 2 cut(s) | 880, 1674 |
| Aor13HI | TCCGGA | 1 cut(s) | 1066 |
| AoxI | GGCC | 2 cut(s) | 2070, 2099 |
| ApeKI | GCWGC | 2 cut(s) | 275, 867 |
| ApoI | RAATTY | 6 cut(s) | 443, 516, 587, 599, 1158, 1316 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2114, 2146 |
| AseI | ATTAAT | 1 cut(s) | 422 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 126, 334, 1485 |
| AspA2I | CCTAGG | 1 cut(s) | 574 |
| AspS9I | GGNCC | 4 cut(s) | 859, 1028, 1560, 2100 |
| AsuC2I | CCSGG | 3 cut(s) | 955, 1675, 1676 |
| AsuHPI | GGTGA | 7 cut(s) | 367, 453, 464, 1330, 1658, 1690, 2016 |
| AvaI | CYCGRG | 2 cut(s) | 880, 1674 |
| AvaII | GGWCC | 3 cut(s) | 859, 1028, 1560 |
| AvrII | CCTAGG | 1 cut(s) | 574 |
| BamHI | GGATCC | 1 cut(s) | 2142 |
| BanII | GRGCYC | 2 cut(s) | 1740, 1835 |
| Bbv12I | GWGCWC | 3 cut(s) | 1583, 1740, 1835 |
| BbvI | GCAGC | 2 cut(s) | 262, 879 |
| BccI | CCATC | 7 cut(s) | 179, 414, 462, 973, 1567, 1930, 2035 |
| BceAI | ACGGC | 2 cut(s) | 2114, 2149 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 236, 270 |
| BciT130I | CCWGG | 4 cut(s) | 1570, 1950, 2091, 2140 |
| BcnI | CCSGG | 3 cut(s) | 955, 1675, 1676 |
| BcoDI | GTCTC | 2 cut(s) | 650, 940 |
| BfaI | CTAG | 8 cut(s) | 348, 575, 759, 1250, 1464, 1478, 2006, 2208 |
| BfmI | CTRYAG | 3 cut(s) | 845, 1778, 1857 |
| BfuAI | ACCTGC | 3 cut(s) | 184, 852, 2186 |
| BglII | AGATCT | 1 cut(s) | 1514 |
| BisI | GCNGC | 3 cut(s) | 276, 868, 1045 |
| BlnI | CCTAGG | 1 cut(s) | 574 |
| BlsI | GCNGC | 3 cut(s) | 277, 869, 1046 |
| BmcAI | AGTACT | 1 cut(s) | 851 |
| Bme1390I | CCNGG | 7 cut(s) | 955, 1570, 1675, 1676, 1950, 2091, 2140 |
| Bme18I | GGWCC | 3 cut(s) | 859, 1028, 1560 |
| BmeT110I | CYCGRG | 2 cut(s) | 880, 1674 |
| BmgBI | CACGTC | 1 cut(s) | 209 |
| BmgT120I | GGNCC | 4 cut(s) | 859, 1028, 1560, 2100 |
| BmiI | GGNNCC | 4 cut(s) | 580, 1561, 1884, 2144 |
| BmrFI | CCNGG | 7 cut(s) | 955, 1570, 1675, 1676, 1950, 2091, 2140 |
| BmrI | ACTGGG | 2 cut(s) | 405, 2027 |
| BmsI | GCATC | 2 cut(s) | 256, 1954 |
| BmuI | ACTGGG | 2 cut(s) | 405, 2027 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2020 |
| BpmI | CTGGAG | 2 cut(s) | 941, 1869 |
| Bpu10I | CCTNAGC | 3 cut(s) | 537, 863, 1269 |
| BpuEI | CTTGAG | 2 cut(s) | 30, 725 |
| BpuMI | CCSGG | 3 cut(s) | 955, 1675, 1676 |
| BsaJI | CCNNGG | 3 cut(s) | 574, 1674, 1949 |
| BsaWI | WCCGGW | 2 cut(s) | 377, 1066 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 563, 593, 935, 965 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 470, 542, 1043, 1187, 1569, 1575, 1848, 2017, 2033 |
| Bse1I | ACTGG | 7 cut(s) | 78, 411, 605, 749, 1853, 1965, 2022 |
| Bse3DI | GCAATG | 2 cut(s) | 186, 311 |
| BseAI | TCCGGA | 1 cut(s) | 1066 |
| BseBI | CCWGG | 4 cut(s) | 1570, 1950, 2091, 2140 |
| BseDI | CCNNGG | 3 cut(s) | 574, 1674, 1949 |
| BseGI | GGATG | 5 cut(s) | 190, 406, 1264, 1578, 1945 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 470, 542, 1043, 1187, 1569, 1575, 1848, 2017, 2033 |
| BseMI | GCAATG | 2 cut(s) | 186, 311 |
| BseMII | CTCAG | 7 cut(s) | 309, 528, 655, 661, 1260, 1338, 2151 |
| BseNI | ACTGG | 7 cut(s) | 78, 411, 605, 749, 1853, 1965, 2022 |
| BseRI | GAGGAG | 2 cut(s) | 839, 1395 |
| BseXI | GCAGC | 2 cut(s) | 262, 879 |
| BseYI | CCCAGC | 1 cut(s) | 1665 |
| BshFI | GGCC | 2 cut(s) | 2072, 2101 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1583, 1740, 1835 |
| BsiHKCI | CYCGRG | 2 cut(s) | 880, 1674 |
| BsiSI | CCGG | 5 cut(s) | 355, 378, 954, 1067, 1675 |
| BslFI | GGGAC | 5 cut(s) | 18, 322, 1128, 1682, 1766 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 470, 542, 1043, 1187, 1569, 1575, 1848, 2017, 2033 |
| BsmAI | GTCTC | 2 cut(s) | 650, 940 |
| BsmFI | GGGAC | 5 cut(s) | 18, 322, 1128, 1682, 1766 |
| BsmI | GAATGC | 2 cut(s) | 64, 482 |
| BsnI | GGCC | 2 cut(s) | 2072, 2101 |
| BsoBI | CYCGRG | 2 cut(s) | 880, 1674 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1583, 1740, 1835 |
| Bsp13I | TCCGGA | 1 cut(s) | 1066 |
| Bsp143I | GATC | 7 cut(s) | 884, 1135, 1241, 1261, 1514, 2086, 2142 |
| BspACI | CCGC | 4 cut(s) | 1044, 1341, 1783, 1862 |
| BspANI | GGCC | 2 cut(s) | 2072, 2101 |
| BspCNI | CTCAG | 7 cut(s) | 310, 529, 654, 660, 1261, 1339, 2152 |
| BspEI | TCCGGA | 1 cut(s) | 1066 |
| BspHI | TCATGA | 1 cut(s) | 1932 |
| BspLI | GGNNCC | 4 cut(s) | 580, 1561, 1884, 2144 |
| BspMAI | CTGCAG | 1 cut(s) | 849 |
| BspMI | ACCTGC | 3 cut(s) | 184, 852, 2186 |
| BspPI | GGATC | 5 cut(s) | 892, 1130, 2081, 2137, 2150 |
| BsrDI | GCAATG | 2 cut(s) | 186, 311 |
| BsrI | ACTGG | 7 cut(s) | 78, 411, 605, 749, 1853, 1965, 2022 |
| BssECI | CCNNGG | 3 cut(s) | 574, 1674, 1949 |
| BssMI | GATC | 7 cut(s) | 884, 1135, 1241, 1261, 1514, 2086, 2142 |
| BssNAI | GTATAC | 1 cut(s) | 1903 |
| BssT1I | CCWWGG | 1 cut(s) | 574 |
| Bst1107I | GTATAC | 1 cut(s) | 1903 |
| Bst2UI | CCWGG | 4 cut(s) | 1570, 1950, 2091, 2140 |
| Bst4CI | ACNGT | 2 cut(s) | 197, 1827 |
| Bst6I | CTCTTC | 3 cut(s) | 99, 1300, 1372 |
| BstC8I | GCNNGC | 2 cut(s) | 155, 1275 |
| BstDEI | CTNAG | 9 cut(s) | 318, 537, 623, 641, 647, 863, 1269, 1347, 2160 |
| BstEII | GGTNACC | 1 cut(s) | 2022 |
| BstF5I | GGATG | 5 cut(s) | 190, 406, 1264, 1578, 1945 |
| BstKTI | GATC | 7 cut(s) | 887, 1138, 1244, 1264, 1517, 2089, 2145 |
| BstMAI | GTCTC | 2 cut(s) | 650, 940 |
| BstMBI | GATC | 7 cut(s) | 884, 1135, 1241, 1261, 1514, 2086, 2142 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 916, 2107 |
| BstNI | CCWGG | 4 cut(s) | 1570, 1950, 2091, 2140 |
| BstNSI | RCATGY | 1 cut(s) | 1706 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2020 |
| BstPI | GGTNACC | 1 cut(s) | 2022 |
| BstSCI | CCNGG | 7 cut(s) | 953, 1568, 1673, 1674, 1948, 2089, 2138 |
| BstSFI | CTRYAG | 3 cut(s) | 845, 1778, 1857 |
| BstV1I | GCAGC | 2 cut(s) | 262, 879 |
| BstX2I | RGATCY | 3 cut(s) | 1135, 1514, 2142 |
| BstYI | RGATCY | 3 cut(s) | 1135, 1514, 2142 |
| BstZ17I | GTATAC | 1 cut(s) | 1903 |
| BsuRI | GGCC | 2 cut(s) | 2072, 2101 |
| BtrI | CACGTC | 1 cut(s) | 209 |
| BtsCI | GGATG | 5 cut(s) | 190, 406, 1264, 1578, 1945 |
| BtsIMutI | CAGTG | 4 cut(s) | 202, 598, 1344, 2082 |
| BveI | ACCTGC | 3 cut(s) | 184, 852, 2186 |
| Cac8I | GCNNGC | 2 cut(s) | 155, 1275 |
| CciI | TCATGA | 1 cut(s) | 1932 |
| Cfr13I | GGNCC | 4 cut(s) | 859, 1028, 1560, 2100 |
| Cfr9I | CCCGGG | 1 cut(s) | 1674 |
| Csp6I | GTAC | 5 cut(s) | 204, 850, 1366, 1585, 2076 |
| CviAII | CATG | 7 cut(s) | 161, 1125, 1703, 1797, 1879, 1933, 2173 |
| CviQI | GTAC | 5 cut(s) | 204, 850, 1366, 1585, 2076 |
| DdeI | CTNAG | 9 cut(s) | 318, 537, 623, 641, 647, 863, 1269, 1347, 2160 |
| DpnI | GATC | 7 cut(s) | 886, 1137, 1243, 1263, 1516, 2088, 2144 |
| DpnII | GATC | 7 cut(s) | 884, 1135, 1241, 1261, 1514, 2086, 2142 |
| DraIII | CACNNNGTG | 1 cut(s) | 199 |
| Eam1104I | CTCTTC | 3 cut(s) | 99, 1300, 1372 |
| EarI | CTCTTC | 3 cut(s) | 99, 1300, 1372 |
| Ecl136II | GAGCTC | 2 cut(s) | 1738, 1833 |
| Eco130I | CCWWGG | 1 cut(s) | 574 |
| Eco24I | GRGCYC | 2 cut(s) | 1740, 1835 |
| Eco32I | GATATC | 1 cut(s) | 52 |
| Eco47I | GGWCC | 3 cut(s) | 859, 1028, 1560 |
| Eco53kI | GAGCTC | 2 cut(s) | 1738, 1833 |
| Eco57I | CTGAAG | 2 cut(s) | 80, 1116 |
| Eco88I | CYCGRG | 2 cut(s) | 880, 1674 |
| Eco91I | GGTNACC | 1 cut(s) | 2022 |
| EcoICRI | GAGCTC | 2 cut(s) | 1738, 1833 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1560 |
| EcoO65I | GGTNACC | 1 cut(s) | 2022 |
| EcoRI | GAATTC | 1 cut(s) | 587 |
| EcoRII | CCWGG | 4 cut(s) | 1568, 1948, 2089, 2138 |
| EcoRV | GATATC | 1 cut(s) | 52 |
| EcoT14I | CCWWGG | 1 cut(s) | 574 |
| EcoT22I | ATGCAT | 1 cut(s) | 397 |
| EcoT38I | GRGCYC | 2 cut(s) | 1740, 1835 |
| ErhI | CCWWGG | 1 cut(s) | 574 |
| FaeI | CATG | 7 cut(s) | 164, 1128, 1706, 1800, 1882, 1936, 2176 |
| FaqI | GGGAC | 5 cut(s) | 18, 322, 1128, 1682, 1766 |
| FatI | CATG | 7 cut(s) | 160, 1124, 1702, 1796, 1878, 1932, 2172 |
| FauI | CCCGC | 1 cut(s) | 1776 |
| FblI | GTMKAC | 4 cut(s) | 753, 996, 1398, 1902 |
| Fnu4HI | GCNGC | 3 cut(s) | 276, 868, 1045 |
| FokI | GGATG | 5 cut(s) | 197, 393, 1251, 1585, 1932 |
| FriOI | GRGCYC | 2 cut(s) | 1740, 1835 |
| Fsp4HI | GCNGC | 3 cut(s) | 276, 868, 1045 |
| FspBI | CTAG | 8 cut(s) | 348, 575, 759, 1250, 1464, 1478, 2006, 2208 |
| GluI | GCNGC | 3 cut(s) | 276, 868, 1045 |
| GsaI | CCCAGC | 1 cut(s) | 1669 |
| GsuI | CTGGAG | 2 cut(s) | 941, 1869 |
| HaeIII | GGCC | 2 cut(s) | 2072, 2101 |
| HapII | CCGG | 5 cut(s) | 355, 378, 954, 1067, 1675 |
| Hin1II | CATG | 7 cut(s) | 164, 1128, 1706, 1800, 1882, 1936, 2176 |
| HincII | GTYRAC | 4 cut(s) | 191, 262, 777, 997 |
| HindII | GTYRAC | 4 cut(s) | 191, 262, 777, 997 |
| HindIII | AAGCTT | 3 cut(s) | 625, 781, 908 |
| HinfI | GANTC | 8 cut(s) | 351, 619, 925, 1289, 1460, 1486, 1649, 1838 |
| HpaI | GTTAAC | 1 cut(s) | 777 |
| HpaII | CCGG | 5 cut(s) | 355, 378, 954, 1067, 1675 |
| HphI | GGTGA | 7 cut(s) | 367, 453, 464, 1330, 1658, 1690, 2016 |
| Hpy99I | CGWCG | 2 cut(s) | 213, 216 |
| HpyAV | CCTTC | 2 cut(s) | 1935, 1990 |
| HpyCH4III | ACNGT | 2 cut(s) | 197, 1827 |
| HpyCH4IV | ACGT | 2 cut(s) | 208, 764 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 916, 2107 |
| HpyF3I | CTNAG | 9 cut(s) | 318, 537, 623, 641, 647, 863, 1269, 1347, 2160 |
| HpySE526I | ACGT | 2 cut(s) | 208, 764 |
| Hsp92II | CATG | 7 cut(s) | 164, 1128, 1706, 1800, 1882, 1936, 2176 |
| Kpn2I | TCCGGA | 1 cut(s) | 1066 |
| KspAI | GTTAAC | 1 cut(s) | 777 |
| Kzo9I | GATC | 7 cut(s) | 884, 1135, 1241, 1261, 1514, 2086, 2142 |
| LmnI | GCTCC | 3 cut(s) | 584, 1736, 1888 |
| Lsp1109I | GCAGC | 2 cut(s) | 262, 879 |
| LweI | GCATC | 2 cut(s) | 256, 1954 |
| MaeI | CTAG | 8 cut(s) | 348, 575, 759, 1250, 1464, 1478, 2006, 2208 |
| MaeII | ACGT | 2 cut(s) | 208, 764 |
| MaeIII | GTNAC | 7 cut(s) | 79, 1204, 1219, 1466, 2022, 2062, 2213 |
| MalI | GATC | 7 cut(s) | 886, 1137, 1243, 1263, 1516, 2088, 2144 |
| MboI | GATC | 7 cut(s) | 884, 1135, 1241, 1261, 1514, 2086, 2142 |
| MboII | GAAGA | 5 cut(s) | 86, 1154, 1317, 1322, 1389 |
| MfeI | CAATTG | 2 cut(s) | 240, 557 |
| MflI | RGATCY | 3 cut(s) | 1135, 1514, 2142 |
| MhlI | GDGCHC | 3 cut(s) | 1583, 1740, 1835 |
| MlyI | GAGTC | 1 cut(s) | 360 |
| MmeI | TCCRAC | 2 cut(s) | 837, 1063 |
| Mph1103I | ATGCAT | 1 cut(s) | 397 |
| MroI | TCCGGA | 1 cut(s) | 1066 |
| MroXI | GAANNNNTTC | 3 cut(s) | 126, 334, 1485 |
| MseI | TTAA | 9 cut(s) | 302, 422, 551, 776, 896, 930, 1227, 1437, 2184 |
| MslI | CAYNNNNRTG | 5 cut(s) | 255, 466, 494, 1539, 1676 |
| MspA1I | CMGCKG | 1 cut(s) | 1783 |
| MspI | CCGG | 5 cut(s) | 355, 378, 954, 1067, 1675 |
| MspR9I | CCNGG | 7 cut(s) | 955, 1570, 1675, 1676, 1950, 2091, 2140 |
| MunI | CAATTG | 2 cut(s) | 240, 557 |
| Mva1269I | GAATGC | 2 cut(s) | 64, 482 |
| MvaI | CCWGG | 4 cut(s) | 1570, 1950, 2091, 2140 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 916, 2107 |
| NciI | CCSGG | 3 cut(s) | 955, 1675, 1676 |
| NdeII | GATC | 7 cut(s) | 884, 1135, 1241, 1261, 1514, 2086, 2142 |
| NlaIII | CATG | 7 cut(s) | 164, 1128, 1706, 1800, 1882, 1936, 2176 |
| NlaIV | GGNNCC | 4 cut(s) | 580, 1561, 1884, 2144 |
| NmuCI | GTSAC | 5 cut(s) | 1204, 1219, 1466, 2022, 2213 |
| NsiI | ATGCAT | 1 cut(s) | 397 |
| NspI | RCATGY | 1 cut(s) | 1706 |
| OliI | CACNNNNGTG | 1 cut(s) | 1676 |
| PagI | TCATGA | 1 cut(s) | 1932 |
| PaqCI | CACCTGC | 1 cut(s) | 852 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1589 |
| PctI | GAATGC | 2 cut(s) | 64, 482 |
| PdmI | GAANNNNTTC | 3 cut(s) | 126, 334, 1485 |
| PfeI | GAWTC | 7 cut(s) | 619, 925, 1289, 1460, 1486, 1649, 1838 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 2017, 2033 |
| PfoI | TCCNGGA | 2 cut(s) | 953, 1568 |
| PkrI | GCNGC | 3 cut(s) | 277, 869, 1046 |
| PleI | GAGTC | 1 cut(s) | 359 |
| PpsI | GAGTC | 1 cut(s) | 359 |
| PpuMI | RGGWCCY | 1 cut(s) | 1560 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2020 |
| PshBI | ATTAAT | 1 cut(s) | 422 |
| PsiI | TTATAA | 1 cut(s) | 914 |
| Psp124BI | GAGCTC | 2 cut(s) | 1740, 1835 |
| Psp1406I | AACGTT | 1 cut(s) | 764 |
| Psp5II | RGGWCCY | 1 cut(s) | 1560 |
| Psp6I | CCWGG | 4 cut(s) | 1568, 1948, 2089, 2138 |
| PspEI | GGTNACC | 1 cut(s) | 2022 |
| PspFI | CCCAGC | 1 cut(s) | 1665 |
| PspGI | CCWGG | 4 cut(s) | 1568, 1948, 2089, 2138 |
| PspN4I | GGNNCC | 4 cut(s) | 580, 1561, 1884, 2144 |
| PspPI | GGNCC | 4 cut(s) | 859, 1028, 1560, 2100 |
| PspPPI | RGGWCCY | 1 cut(s) | 1560 |
| PstI | CTGCAG | 1 cut(s) | 849 |
| PsuI | RGATCY | 3 cut(s) | 1135, 1514, 2142 |
| RsaI | GTAC | 5 cut(s) | 205, 851, 1367, 1586, 2077 |
| RsaNI | GTAC | 5 cut(s) | 204, 850, 1366, 1585, 2076 |
| RseI | CAYNNNNRTG | 5 cut(s) | 255, 466, 494, 1539, 1676 |
| SacI | GAGCTC | 2 cut(s) | 1740, 1835 |
| SalI | GTCGAC | 1 cut(s) | 995 |
| SaqAI | TTAA | 9 cut(s) | 302, 422, 551, 776, 896, 930, 1227, 1437, 2184 |
| SatI | GCNGC | 3 cut(s) | 276, 868, 1045 |
| Sau3AI | GATC | 7 cut(s) | 884, 1135, 1241, 1261, 1514, 2086, 2142 |
| Sau96I | GGNCC | 4 cut(s) | 859, 1028, 1560, 2100 |
| ScaI | AGTACT | 1 cut(s) | 851 |
| SchI | GAGTC | 1 cut(s) | 360 |
| ScrFI | CCNGG | 7 cut(s) | 955, 1570, 1675, 1676, 1950, 2091, 2140 |
| SduI | GDGCHC | 3 cut(s) | 1583, 1740, 1835 |
| SfaNI | GCATC | 2 cut(s) | 256, 1954 |
| SfcI | CTRYAG | 3 cut(s) | 845, 1778, 1857 |
| SinI | GGWCC | 3 cut(s) | 859, 1028, 1560 |
| SmaI | CCCGGG | 1 cut(s) | 1676 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 255, 466, 494, 1539, 1676 |
| SmlI | CTYRAG | 2 cut(s) | 45, 704 |
| SmoI | CTYRAG | 2 cut(s) | 45, 704 |
| SsiI | CCGC | 4 cut(s) | 1044, 1341, 1783, 1862 |
| SspMI | CTAG | 8 cut(s) | 348, 575, 759, 1250, 1464, 1478, 2006, 2208 |
| SstI | GAGCTC | 2 cut(s) | 1740, 1835 |
| StyD4I | CCNGG | 7 cut(s) | 953, 1568, 1673, 1674, 1948, 2089, 2138 |
| StyI | CCWWGG | 1 cut(s) | 574 |
| TaaI | ACNGT | 2 cut(s) | 197, 1827 |
| TaiI | ACGT | 2 cut(s) | 211, 767 |
| TaqI | TCGA | 3 cut(s) | 25, 996, 1500 |
| TatI | WGTACW | 2 cut(s) | 203, 849 |
| TauI | GCSGC | 1 cut(s) | 1047 |
| TfiI | GAWTC | 7 cut(s) | 619, 925, 1289, 1460, 1486, 1649, 1838 |
| Tru1I | TTAA | 9 cut(s) | 302, 422, 551, 776, 896, 930, 1227, 1437, 2184 |
| Tru9I | TTAA | 9 cut(s) | 302, 422, 551, 776, 896, 930, 1227, 1437, 2184 |
| TscAI | CASTG | 4 cut(s) | 202, 598, 1351, 2089 |
| TseFI | GTSAC | 5 cut(s) | 1204, 1219, 1466, 2022, 2213 |
| TseI | GCWGC | 2 cut(s) | 275, 867 |
| Tsp45I | GTSAC | 5 cut(s) | 1204, 1219, 1466, 2022, 2213 |
| TspDTI | ATGAA | 5 cut(s) | 378, 600, 1374, 1460, 1611 |
| TspGWI | ACGGA | 2 cut(s) | 506, 522 |
| TspMI | CCCGGG | 1 cut(s) | 1674 |
| TspRI | CASTG | 4 cut(s) | 202, 598, 1351, 2089 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 2017, 2033 |
| VpaK11BI | GGWCC | 3 cut(s) | 859, 1028, 1560 |
| VspI | ATTAAT | 1 cut(s) | 422 |
| XapI | RAATTY | 6 cut(s) | 443, 516, 587, 599, 1158, 1316 |
| XbaI | TCTAGA | 2 cut(s) | 347, 1477 |
| XceI | RCATGY | 1 cut(s) | 1706 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2065 |
| XmaI | CCCGGG | 1 cut(s) | 1674 |
| XmaJI | CCTAGG | 1 cut(s) | 574 |
| XmiI | GTMKAC | 4 cut(s) | 753, 996, 1398, 1902 |
| XmnI | GAANNNNTTC | 3 cut(s) | 126, 334, 1485 |
| XspI | CTAG | 8 cut(s) | 348, 575, 759, 1250, 1464, 1478, 2006, 2208 |
| ZrmI | AGTACT | 1 cut(s) | 851 |
| Zsp2I | ATGCAT | 1 cut(s) | 397 |