Belongs to the protein kinase superfamily. Ser Thr protein kinase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 438 |
| AccB1I | GGYRCC | 1 cut(s) | 438 |
| AccI | GTMKAC | 1 cut(s) | 259 |
| AccII | CGCG | 1 cut(s) | 111 |
| AciI | CCGC | 2 cut(s) | 109, 202 |
| AcoI | YGGCCR | 1 cut(s) | 314 |
| AcsI | RAATTY | 2 cut(s) | 382, 452 |
| AfaI | GTAC | 2 cut(s) | 123, 440 |
| AgeI | ACCGGT | 1 cut(s) | 507 |
| AjnI | CCWGG | 2 cut(s) | 303, 409 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 33, 65, 359, 391 |
| AluBI | AGCT | 5 cut(s) | 73, 116, 329, 337, 390 |
| AluI | AGCT | 5 cut(s) | 73, 116, 329, 337, 390 |
| Alw26I | GTCTC | 2 cut(s) | 471, 494 |
| AoxI | GGCC | 2 cut(s) | 80, 314 |
| ApeKI | GCWGC | 2 cut(s) | 247, 295 |
| ApoI | RAATTY | 2 cut(s) | 382, 452 |
| AsiGI | ACCGGT | 1 cut(s) | 507 |
| Asp718I | GGTACC | 1 cut(s) | 438 |
| AspLEI | GCGC | 1 cut(s) | 180 |
| AspS9I | GGNCC | 3 cut(s) | 81, 413, 510 |
| AsuHPI | GGTGA | 3 cut(s) | 212, 281, 353 |
| AvaII | GGWCC | 2 cut(s) | 413, 510 |
| AxyI | CCTNAGG | 1 cut(s) | 19 |
| BalI | TGGCCA | 1 cut(s) | 316 |
| BanI | GGYRCC | 1 cut(s) | 438 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 105, 137 |
| BbvCI | CCTCAGC | 2 cut(s) | 60, 345 |
| BbvI | GCAGC | 2 cut(s) | 234, 307 |
| BccI | CCATC | 4 cut(s) | 93, 170, 177, 450 |
| BceAI | ACGGC | 1 cut(s) | 23 |
| BciT130I | CCWGG | 2 cut(s) | 305, 411 |
| BcoDI | GTCTC | 2 cut(s) | 471, 494 |
| BfmI | CTRYAG | 1 cut(s) | 189 |
| BfoI | RGCGCY | 1 cut(s) | 181 |
| BisI | GCNGC | 3 cut(s) | 109, 248, 296 |
| BlsI | GCNGC | 3 cut(s) | 110, 249, 297 |
| Bme1390I | CCNGG | 2 cut(s) | 305, 411 |
| Bme18I | GGWCC | 2 cut(s) | 413, 510 |
| BmgT120I | GGNCC | 3 cut(s) | 81, 413, 510 |
| BmiI | GGNNCC | 3 cut(s) | 32, 83, 440 |
| BmrFI | CCNGG | 2 cut(s) | 305, 411 |
| BpmI | CTGGAG | 2 cut(s) | 102, 495 |
| Bpu10I | CCTNAGC | 2 cut(s) | 60, 345 |
| BpuEI | CTTGAG | 1 cut(s) | 359 |
| BsaI | GGTCTC | 1 cut(s) | 471 |
| BsaJI | CCNNGG | 3 cut(s) | 303, 304, 375 |
| BsaWI | WCCGGW | 4 cut(s) | 222, 363, 435, 507 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 60, 90, 417, 447 |
| Bse118I | RCCGGY | 2 cut(s) | 291, 507 |
| Bse1I | ACTGG | 1 cut(s) | 119 |
| Bse21I | CCTNAGG | 1 cut(s) | 19 |
| Bse3DI | GCAATG | 1 cut(s) | 110 |
| BseBI | CCWGG | 2 cut(s) | 305, 411 |
| BseDI | CCNNGG | 3 cut(s) | 303, 304, 375 |
| BseGI | GGATG | 2 cut(s) | 85, 442 |
| BseMI | GCAATG | 1 cut(s) | 110 |
| BseMII | CTCAG | 4 cut(s) | 33, 74, 324, 359 |
| BseNI | ACTGG | 1 cut(s) | 119 |
| BseRI | GAGGAG | 1 cut(s) | 147 |
| BseXI | GCAGC | 2 cut(s) | 234, 307 |
| Bsh1236I | CGCG | 1 cut(s) | 111 |
| BshFI | GGCC | 2 cut(s) | 82, 316 |
| BshNI | GGYRCC | 1 cut(s) | 438 |
| BshTI | ACCGGT | 1 cut(s) | 507 |
| BsiSI | CCGG | 7 cut(s) | 79, 223, 292, 350, 364, 436, 508 |
| BslFI | GGGAC | 1 cut(s) | 321 |
| BsmAI | GTCTC | 2 cut(s) | 471, 494 |
| BsmBI | CGTCTC | 1 cut(s) | 494 |
| BsmFI | GGGAC | 1 cut(s) | 321 |
| BsmI | GAATGC | 1 cut(s) | 298 |
| BsnI | GGCC | 2 cut(s) | 82, 316 |
| Bso31I | GGTCTC | 1 cut(s) | 471 |
| Bsp143I | GATC | 2 cut(s) | 95, 265 |
| BspACI | CCGC | 2 cut(s) | 109, 202 |
| BspANI | GGCC | 2 cut(s) | 82, 316 |
| BspCNI | CTCAG | 4 cut(s) | 32, 73, 325, 358 |
| BspFNI | CGCG | 1 cut(s) | 111 |
| BspLI | GGNNCC | 3 cut(s) | 32, 83, 440 |
| BspMAI | CTGCAG | 1 cut(s) | 193 |
| BspT107I | GGYRCC | 1 cut(s) | 438 |
| BspTNI | GGTCTC | 1 cut(s) | 471 |
| BsrDI | GCAATG | 1 cut(s) | 110 |
| BsrFI | RCCGGY | 2 cut(s) | 291, 507 |
| BsrI | ACTGG | 1 cut(s) | 119 |
| BssAI | RCCGGY | 2 cut(s) | 291, 507 |
| BssECI | CCNNGG | 3 cut(s) | 303, 304, 375 |
| BssMI | GATC | 2 cut(s) | 95, 265 |
| BssT1I | CCWWGG | 1 cut(s) | 375 |
| Bst2UI | CCWGG | 2 cut(s) | 305, 411 |
| BstDEI | CTNAG | 4 cut(s) | 19, 60, 333, 345 |
| BstF5I | GGATG | 2 cut(s) | 85, 442 |
| BstFNI | CGCG | 1 cut(s) | 111 |
| BstH2I | RGCGCY | 1 cut(s) | 181 |
| BstHHI | GCGC | 1 cut(s) | 180 |
| BstKTI | GATC | 2 cut(s) | 98, 268 |
| BstMAI | GTCTC | 2 cut(s) | 471, 494 |
| BstMBI | GATC | 2 cut(s) | 95, 265 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 70, 79, 253 |
| BstNI | CCWGG | 2 cut(s) | 305, 411 |
| BstSCI | CCNGG | 2 cut(s) | 303, 409 |
| BstSFI | CTRYAG | 1 cut(s) | 189 |
| BstUI | CGCG | 1 cut(s) | 111 |
| BstV1I | GCAGC | 2 cut(s) | 234, 307 |
| Bsu36I | CCTNAGG | 1 cut(s) | 19 |
| BsuRI | GGCC | 2 cut(s) | 82, 316 |
| BtsCI | GGATG | 2 cut(s) | 85, 442 |
| BtsI | GCAGTG | 1 cut(s) | 317 |
| BtsIMutI | CAGTG | 1 cut(s) | 317 |
| CfoI | GCGC | 1 cut(s) | 180 |
| Cfr10I | RCCGGY | 2 cut(s) | 291, 507 |
| Cfr13I | GGNCC | 3 cut(s) | 81, 413, 510 |
| CseI | GACGC | 1 cut(s) | 245 |
| Csp6I | GTAC | 2 cut(s) | 122, 439 |
| CspAI | ACCGGT | 1 cut(s) | 507 |
| CviQI | GTAC | 2 cut(s) | 122, 439 |
| DdeI | CTNAG | 4 cut(s) | 19, 60, 333, 345 |
| DpnI | GATC | 2 cut(s) | 97, 267 |
| DpnII | GATC | 2 cut(s) | 95, 265 |
| EaeI | YGGCCR | 1 cut(s) | 314 |
| EciI | GGCGGA | 1 cut(s) | 191 |
| Eco130I | CCWWGG | 1 cut(s) | 375 |
| Eco31I | GGTCTC | 1 cut(s) | 471 |
| Eco47I | GGWCC | 2 cut(s) | 413, 510 |
| Eco81I | CCTNAGG | 1 cut(s) | 19 |
| EcoRII | CCWGG | 2 cut(s) | 303, 409 |
| EcoT14I | CCWWGG | 1 cut(s) | 375 |
| ErhI | CCWWGG | 1 cut(s) | 375 |
| Esp3I | CGTCTC | 1 cut(s) | 494 |
| FaiI | YATR | 2 cut(s) | 372, 402 |
| FaqI | GGGAC | 1 cut(s) | 321 |
| FblI | GTMKAC | 1 cut(s) | 259 |
| Fnu4HI | GCNGC | 3 cut(s) | 109, 248, 296 |
| FokI | GGATG | 2 cut(s) | 72, 429 |
| Fsp4HI | GCNGC | 3 cut(s) | 109, 248, 296 |
| GlaI | GCGC | 1 cut(s) | 179 |
| GluI | GCNGC | 3 cut(s) | 109, 248, 296 |
| GsuI | CTGGAG | 2 cut(s) | 102, 495 |
| HaeII | RGCGCY | 1 cut(s) | 181 |
| HaeIII | GGCC | 2 cut(s) | 82, 316 |
| HapII | CCGG | 7 cut(s) | 79, 223, 292, 350, 364, 436, 508 |
| HgaI | GACGC | 1 cut(s) | 245 |
| HhaI | GCGC | 1 cut(s) | 180 |
| Hin6I | GCGC | 1 cut(s) | 178 |
| HinP1I | GCGC | 1 cut(s) | 178 |
| HincII | GTYRAC | 1 cut(s) | 505 |
| HindII | GTYRAC | 1 cut(s) | 505 |
| HindIII | AAGCTT | 1 cut(s) | 327 |
| HpaI | GTTAAC | 1 cut(s) | 505 |
| HpaII | CCGG | 7 cut(s) | 79, 223, 292, 350, 364, 436, 508 |
| HphI | GGTGA | 3 cut(s) | 212, 281, 353 |
| Hpy166II | GTNNAC | 2 cut(s) | 260, 505 |
| Hpy188I | TCNGA | 2 cut(s) | 95, 334 |
| Hpy188III | TCNNGA | 1 cut(s) | 458 |
| Hpy8I | GTNNAC | 2 cut(s) | 260, 505 |
| HpyAV | CCTTC | 2 cut(s) | 59, 406 |
| HpyCH4IV | ACGT | 1 cut(s) | 487 |
| HpyCH4V | TGCA | 3 cut(s) | 191, 250, 466 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 70, 79, 253 |
| HpyF3I | CTNAG | 4 cut(s) | 19, 60, 333, 345 |
| HpySE526I | ACGT | 1 cut(s) | 487 |
| HspAI | GCGC | 1 cut(s) | 178 |
| KpnI | GGTACC | 1 cut(s) | 442 |
| KspAI | GTTAAC | 1 cut(s) | 505 |
| Kzo9I | GATC | 2 cut(s) | 95, 265 |
| LmnI | GCTCC | 2 cut(s) | 36, 121 |
| Lsp1109I | GCAGC | 2 cut(s) | 234, 307 |
| MaeII | ACGT | 1 cut(s) | 487 |
| MalI | GATC | 2 cut(s) | 97, 267 |
| MboI | GATC | 2 cut(s) | 95, 265 |
| MboII | GAAGA | 1 cut(s) | 44 |
| MlsI | TGGCCA | 1 cut(s) | 316 |
| MluCI | AATT | 5 cut(s) | 270, 367, 382, 452, 461 |
| MluNI | TGGCCA | 1 cut(s) | 316 |
| Mox20I | TGGCCA | 1 cut(s) | 316 |
| MscI | TGGCCA | 1 cut(s) | 316 |
| MseI | TTAA | 5 cut(s) | 4, 184, 484, 496, 504 |
| Msp20I | TGGCCA | 1 cut(s) | 316 |
| MspI | CCGG | 7 cut(s) | 79, 223, 292, 350, 364, 436, 508 |
| MspR9I | CCNGG | 2 cut(s) | 305, 411 |
| Mva1269I | GAATGC | 1 cut(s) | 298 |
| MvaI | CCWGG | 2 cut(s) | 305, 411 |
| MvnI | CGCG | 1 cut(s) | 111 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 70, 79, 253 |
| NdeII | GATC | 2 cut(s) | 95, 265 |
| NlaIV | GGNNCC | 3 cut(s) | 32, 83, 440 |
| NmeAIII | GCCGAG | 1 cut(s) | 79 |
| PasI | CCCWGGG | 1 cut(s) | 304 |
| PctI | GAATGC | 1 cut(s) | 298 |
| PinAI | ACCGGT | 1 cut(s) | 507 |
| PkrI | GCNGC | 3 cut(s) | 110, 249, 297 |
| Psp6I | CCWGG | 2 cut(s) | 303, 409 |
| PspGI | CCWGG | 2 cut(s) | 303, 409 |
| PspN4I | GGNNCC | 3 cut(s) | 32, 83, 440 |
| PspPI | GGNCC | 3 cut(s) | 81, 413, 510 |
| PstI | CTGCAG | 1 cut(s) | 193 |
| RsaI | GTAC | 2 cut(s) | 123, 440 |
| RsaNI | GTAC | 2 cut(s) | 122, 439 |
| SaqAI | TTAA | 5 cut(s) | 4, 184, 484, 496, 504 |
| SatI | GCNGC | 3 cut(s) | 109, 248, 296 |
| Sau3AI | GATC | 2 cut(s) | 95, 265 |
| Sau96I | GGNCC | 3 cut(s) | 81, 413, 510 |
| ScrFI | CCNGG | 2 cut(s) | 305, 411 |
| SfcI | CTRYAG | 1 cut(s) | 189 |
| SinI | GGWCC | 2 cut(s) | 413, 510 |
| SmlI | CTYRAG | 1 cut(s) | 338 |
| SmoI | CTYRAG | 1 cut(s) | 338 |
| Sse9I | AATT | 5 cut(s) | 270, 367, 382, 452, 461 |
| SsiI | CCGC | 2 cut(s) | 109, 202 |
| StyD4I | CCNGG | 2 cut(s) | 303, 409 |
| StyI | CCWWGG | 1 cut(s) | 375 |
| TaiI | ACGT | 1 cut(s) | 490 |
| TaqI | TCGA | 4 cut(s) | 55, 268, 450, 457 |
| TasI | AATT | 5 cut(s) | 270, 367, 382, 452, 461 |
| TauI | GCSGC | 1 cut(s) | 111 |
| Tru1I | TTAA | 5 cut(s) | 4, 184, 484, 496, 504 |
| Tru9I | TTAA | 5 cut(s) | 4, 184, 484, 496, 504 |
| TscAI | CASTG | 1 cut(s) | 324 |
| TseI | GCWGC | 2 cut(s) | 247, 295 |
| TspDTI | ATGAA | 1 cut(s) | 230 |
| TspRI | CASTG | 1 cut(s) | 324 |
| VpaK11BI | GGWCC | 2 cut(s) | 413, 510 |
| XapI | RAATTY | 2 cut(s) | 382, 452 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 169, 407 |
| XmiI | GTMKAC | 1 cut(s) | 259 |