pycom03g14430

Long-chain-alcohol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000676
Physical Location & Seq
Forward (+)
15760649 .. 15761701
1053 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g14430.1

Sequence Viewer

Length: 1053 bp
ATGGAGAGTGAAACCAACAACTTCATCAAGGTATGGATCTTAACCAACATTTCTCTGTGTTATTGTTTCTACATATCATCTAGAATCCCAAAGGGCATCCTCAGGCTCATCTCTCTCCTCCCCATCTTCTATCTCTTCATCATCCTCCCCTTCAACCTCCACTCCTTCCACCTCGGTGGTCCCACCACCTTCTTCCTGGTTTGGTTTGGCATATTCAAGCTCCTCCTCTTCTCCTTTGACCTTGGTCCTCTCTGTGCACCCCCACCAACTCTTTTCCAGTTCATCTCCATAGCTATCCTCCCCATCAAAATCATCCAAAACCCCACAAACTCTGAGAACCCAAATTACCCAGTTGGTAAAACTGTTACAAACATGCCTAAAATGTCGGTTTTGTTTGCTGTAAAAGCCTTGCTTTTGGCTTTGGTCGTTAGGTCATACGACTACAGGCAACATTTACACCCCTATGTCATATTGGCCTTGTACTTCTGCCACATGTACTTGGGGCTAGAAATTGTAATGCCCCTGTGTGCGACCCCGGCTCGAGCTCTTTTCAAGTTTGAACTCGAGCCGCAGTCCAACAAGCCTTACCTTTCCACTTCCCTTCAAGACTTTTGGGGCCGTAGATGGAACCTCATGGTCACAAATATTCTACGGCCTACCACGTATGATCCAGTGCTCCGTATTTCGAAGCATGTGGTCGGGCTTCGGTGGGCCCGATTTCTCGCTCTGATGTCTGCTTTTGCAGTCTCAGGTCTAATGCATGAGGTGATTTATTATTATCTTACACGTGTATCTCCTACATGGGAAGTGACGTGGTTCTTTGTGCTACATGGGTTGTGCGTGGCAATCGAGGTGGAGGTGAAGAAGGCGGTGGCCGAGAGATGGCGGTTTCACCCGTTGGTTTCGGGGCCGTTGACGTTGGGGTTCTTGGCTGTGACAGCCAATTGGCTGTTCTTCCCTCAGCTGCTGAGAAATGGCGTGGATGTCAAGGCTATAGATGAGTATCCAGTTATGGTAGATTTTGTCAAGGCACACATGCCCTTACTGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBOAT_2

Protein Analysis

351

Amino Acids

40.3

Weight (kDa)

9.31

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT_2 PF13813 189 - 275 3.4e-19 Membrane bound O-acyl transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 569, 869, 886
AclWI GGATC 2 cut(s) 44, 662
AcoI YGGCCR 1 cut(s) 873
AcvI CACGTG 1 cut(s) 788
AfaI GTAC 2 cut(s) 482, 497
AfiI CCNNNNNNNGG 1 cut(s) 882
AflIII ACRYGT 3 cut(s) 492, 785, 787
AgsI TTSAA 5 cut(s) 154, 217, 553, 560, 605
AjiI CACGTC 1 cut(s) 813
AjnI CCWGG 1 cut(s) 195
AjuI GAANNNNNNNTTGG 2 cut(s) 935, 967
AleI CACNNNNGTG 1 cut(s) 174
AluBI AGCT 4 cut(s) 220, 293, 545, 964
AluI AGCT 4 cut(s) 220, 293, 545, 964
Alw21I GWGCWC 3 cut(s) 259, 547, 678
Alw26I GTCTC 1 cut(s) 751
Alw44I GTGCAC 1 cut(s) 255
AlwI GGATC 2 cut(s) 44, 662
AlwNI CAGNNNCTG 1 cut(s) 967
Ama87I CYCGRG 2 cut(s) 540, 563
AoxI GGCC 6 cut(s) 474, 616, 653, 711, 873, 908
ApaI GGGCCC 1 cut(s) 715
ApaLI GTGCAC 1 cut(s) 255
ApeKI GCWGC 1 cut(s) 964
AspS9I GGNCC 6 cut(s) 179, 245, 616, 711, 712, 908
AsuC2I CCSGG 1 cut(s) 536
AsuHPI GGTGA 3 cut(s) 778, 871, 884
AsuII TTCGAA 1 cut(s) 686
AvaI CYCGRG 2 cut(s) 540, 563
AvaII GGWCC 2 cut(s) 179, 245
AxyI CCTNAGG 1 cut(s) 101
BaeGI GKGCMC 2 cut(s) 259, 715
BaeI ACNNNNGTAYC 2 cut(s) 774, 807
BanII GRGCYC 2 cut(s) 547, 715
BbrPI CACGTG 1 cut(s) 788
Bbv12I GWGCWC 3 cut(s) 259, 547, 678
BbvCI CCTCAGC 1 cut(s) 960
BbvI GCAGC 1 cut(s) 951
BccI CCATC 4 cut(s) 131, 311, 618, 876
BceAI ACGGC 3 cut(s) 603, 668, 895
BciT130I CCWGG 1 cut(s) 197
BciVI GTATCC 1 cut(s) 1014
BcnI CCSGG 1 cut(s) 536
BcoDI GTCTC 1 cut(s) 751
BfaI CTAG 2 cut(s) 81, 506
BfmI CTRYAG 2 cut(s) 442, 993
BfuI GTATCC 1 cut(s) 1014
BisI GCNGC 2 cut(s) 569, 965
BlsI GCNGC 2 cut(s) 570, 966
Bme1390I CCNGG 2 cut(s) 197, 536
Bme18I GGWCC 2 cut(s) 179, 245
BmeT110I CYCGRG 2 cut(s) 540, 563
BmgBI CACGTC 1 cut(s) 813
BmgT120I GGNCC 6 cut(s) 179, 245, 616, 711, 712, 908
BmiI GGNNCC 5 cut(s) 181, 617, 629, 713, 909
BmrFI CCNGG 2 cut(s) 197, 536
BmrI ACTGGG 1 cut(s) 344
BmsI GCATC 1 cut(s) 105
BmuI ACTGGG 1 cut(s) 344
BoxI GACNNNNGTC 1 cut(s) 243
Bpu10I CCTNAGC 1 cut(s) 960
Bpu14I TTCGAA 1 cut(s) 686
BpuMI CCSGG 1 cut(s) 536
BsaAI YACGTR 2 cut(s) 663, 788
BsaBI GATNNNNATC 1 cut(s) 1002
BsaJI CCNNGG 3 cut(s) 172, 241, 534
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 1 cut(s) 882
Bse1I ACTGG 4 cut(s) 277, 350, 671, 1007
Bse21I CCTNAGG 1 cut(s) 101
Bse8I GATNNNNATC 1 cut(s) 1002
BseBI CCWGG 1 cut(s) 197
BseDI CCNNGG 3 cut(s) 172, 241, 534
BseGI GGATG 4 cut(s) 96, 141, 312, 988
BseJI GATNNNNATC 1 cut(s) 1002
BseLI CCNNNNNNNGG 1 cut(s) 882
BseMII CTCAG 5 cut(s) 115, 324, 762, 959, 974
BseNI ACTGG 4 cut(s) 277, 350, 671, 1007
BseRI GAGGAG 3 cut(s) 107, 212, 215
BseSI GKGCMC 2 cut(s) 259, 715
BseXI GCAGC 1 cut(s) 951
BshFI GGCC 6 cut(s) 476, 618, 655, 713, 875, 910
BsiHKAI GWGCWC 3 cut(s) 259, 547, 678
BsiHKCI CYCGRG 2 cut(s) 540, 563
BsiSI CCGG 1 cut(s) 536
BslFI GGGAC 1 cut(s) 165
BslI CCNNNNNNNGG 1 cut(s) 882
BsmAI GTCTC 1 cut(s) 751
BsmFI GGGAC 1 cut(s) 165
BsnI GGCC 6 cut(s) 476, 618, 655, 713, 875, 910
BsoBI CYCGRG 2 cut(s) 540, 563
Bsp119I TTCGAA 1 cut(s) 686
Bsp120I GGGCCC 1 cut(s) 711
Bsp1286I GDGCHC 4 cut(s) 259, 547, 678, 715
Bsp143I GATC 2 cut(s) 36, 667
BspACI CCGC 3 cut(s) 569, 869, 886
BspANI GGCC 6 cut(s) 476, 618, 655, 713, 875, 910
BspCNI CTCAG 5 cut(s) 114, 325, 761, 960, 973
BspHI TCATGA 1 cut(s) 1049
BspLI GGNNCC 5 cut(s) 181, 617, 629, 713, 909
BspPI GGATC 2 cut(s) 44, 662
BspT104I TTCGAA 1 cut(s) 686
BsrI ACTGG 4 cut(s) 277, 350, 671, 1007
BssECI CCNNGG 3 cut(s) 172, 241, 534
BssMI GATC 2 cut(s) 36, 667
BssT1I CCWWGG 1 cut(s) 241
Bst2UI CCWGG 1 cut(s) 197
Bst4CI ACNGT 2 cut(s) 364, 1047
Bst6I CTCTTC 2 cut(s) 140, 233
BstBAI YACGTR 2 cut(s) 663, 788
BstBI TTCGAA 1 cut(s) 686
BstDEI CTNAG 5 cut(s) 101, 333, 748, 960, 968
BstF5I GGATG 4 cut(s) 96, 141, 312, 988
BstKTI GATC 2 cut(s) 39, 670
BstMAI GTCTC 1 cut(s) 751
BstMBI GATC 2 cut(s) 36, 667
BstMWI GCNNNNNNNGC 3 cut(s) 404, 536, 938
BstNI CCWGG 1 cut(s) 197
BstNSI RCATGY 4 cut(s) 376, 496, 695, 1039
BstPAI GACNNNNGTC 1 cut(s) 243
BstSCI CCNGG 2 cut(s) 195, 534
BstSFI CTRYAG 2 cut(s) 442, 993
BstSLI GKGCMC 2 cut(s) 259, 715
BstV1I GCAGC 1 cut(s) 951
BstX2I RGATCY 1 cut(s) 36
BstXI CCANNNNNNTGG 1 cut(s) 176
BstYI RGATCY 1 cut(s) 36
Bsu36I CCTNAGG 1 cut(s) 101
BsuI GTATCC 1 cut(s) 1014
BsuRI GGCC 6 cut(s) 476, 618, 655, 713, 875, 910
BtrI CACGTC 1 cut(s) 813
BtsCI GGATG 4 cut(s) 96, 141, 312, 988
BtsIMutI CAGTG 1 cut(s) 678
CaiI CAGNNNCTG 1 cut(s) 967
CciI TCATGA 1 cut(s) 1049
Cfr13I GGNCC 6 cut(s) 179, 245, 616, 711, 712, 908
Csp6I GTAC 2 cut(s) 481, 496
CspCI CAANNNNNGTGG 2 cut(s) 834, 869
CviAII CATG 9 cut(s) 373, 493, 634, 692, 761, 801, 830, 1036, 1050
CviQI GTAC 2 cut(s) 481, 496
DdeI CTNAG 5 cut(s) 101, 333, 748, 960, 968
DpnI GATC 2 cut(s) 38, 669
DpnII GATC 2 cut(s) 36, 667
EaeI YGGCCR 1 cut(s) 873
Eam1104I CTCTTC 2 cut(s) 140, 233
EarI CTCTTC 2 cut(s) 140, 233
Ecl136II GAGCTC 1 cut(s) 545
Eco130I CCWWGG 1 cut(s) 241
Eco24I GRGCYC 2 cut(s) 547, 715
Eco47I GGWCC 2 cut(s) 179, 245
Eco53kI GAGCTC 1 cut(s) 545
Eco72I CACGTG 1 cut(s) 788
Eco81I CCTNAGG 1 cut(s) 101
Eco88I CYCGRG 2 cut(s) 540, 563
EcoICRI GAGCTC 1 cut(s) 545
EcoRII CCWGG 1 cut(s) 195
EcoT14I CCWWGG 1 cut(s) 241
EcoT22I ATGCAT 1 cut(s) 762
EcoT38I GRGCYC 2 cut(s) 547, 715
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 9 cut(s) 376, 496, 637, 695, 764, 804, 833, 1039, 1053
FalI AAGNNNNNCTT 2 cut(s) 396, 428
FaqI GGGAC 1 cut(s) 165
FatI CATG 9 cut(s) 372, 492, 633, 691, 760, 800, 829, 1035, 1049
Fnu4HI GCNGC 2 cut(s) 569, 965
FokI GGATG 4 cut(s) 83, 128, 299, 995
FriOI GRGCYC 2 cut(s) 547, 715
Fsp4HI GCNGC 2 cut(s) 569, 965
FspBI CTAG 2 cut(s) 81, 506
GluI GCNGC 2 cut(s) 569, 965
HaeIII GGCC 6 cut(s) 476, 618, 655, 713, 875, 910
HapII CCGG 1 cut(s) 536
Hin1II CATG 9 cut(s) 376, 496, 637, 695, 764, 804, 833, 1039, 1053
HincII GTYRAC 1 cut(s) 915
HindII GTYRAC 1 cut(s) 915
HinfI GANTC 1 cut(s) 84
HpaII CCGG 1 cut(s) 536
HphI GGTGA 3 cut(s) 778, 871, 884
Hpy166II GTNNAC 2 cut(s) 257, 915
Hpy188I TCNGA 2 cut(s) 334, 729
Hpy188III TCNNGA 3 cut(s) 81, 605, 1050
Hpy8I GTNNAC 2 cut(s) 257, 915
HpyAV CCTTC 5 cut(s) 160, 175, 199, 611, 859
HpyCH4III ACNGT 2 cut(s) 364, 1047
HpyCH4IV ACGT 4 cut(s) 662, 787, 812, 917
HpyCH4V TGCA 3 cut(s) 257, 743, 760
HpyF10VI GCNNNNNNNGC 3 cut(s) 404, 536, 938
HpyF3I CTNAG 5 cut(s) 101, 333, 748, 960, 968
HpySE526I ACGT 4 cut(s) 662, 787, 812, 917
Hsp92II CATG 9 cut(s) 376, 496, 637, 695, 764, 804, 833, 1039, 1053
Kzo9I GATC 2 cut(s) 36, 667
LmnI GCTCC 2 cut(s) 225, 681
Lsp1109I GCAGC 1 cut(s) 951
LweI GCATC 1 cut(s) 105
MaeI CTAG 2 cut(s) 81, 506
MaeII ACGT 4 cut(s) 662, 787, 812, 917
MaeIII GTNAC 4 cut(s) 364, 637, 808, 934
MalI GATC 2 cut(s) 38, 669
MboI GATC 2 cut(s) 36, 667
MboII GAAGA 6 cut(s) 118, 127, 184, 220, 874, 946
MfeI CAATTG 1 cut(s) 943
MflI RGATCY 1 cut(s) 36
MhlI GDGCHC 4 cut(s) 259, 547, 678, 715
MluCI AATT 3 cut(s) 343, 510, 943
MmeI TCCRAC 1 cut(s) 600
Mph1103I ATGCAT 1 cut(s) 762
MseI TTAA 1 cut(s) 41
MslI CAYNNNNRTG 2 cut(s) 174, 462
MspA1I CMGCKG 1 cut(s) 964
MspI CCGG 1 cut(s) 536
MspR9I CCNGG 2 cut(s) 197, 536
MunI CAATTG 1 cut(s) 943
MvaI CCWGG 1 cut(s) 197
MwoI GCNNNNNNNGC 3 cut(s) 404, 536, 938
NciI CCSGG 1 cut(s) 536
NdeII GATC 2 cut(s) 36, 667
NlaIII CATG 9 cut(s) 376, 496, 637, 695, 764, 804, 833, 1039, 1053
NlaIV GGNNCC 5 cut(s) 181, 617, 629, 713, 909
NmeAIII GCCGAG 1 cut(s) 901
NmuCI GTSAC 3 cut(s) 637, 808, 934
NsiI ATGCAT 1 cut(s) 762
NspI RCATGY 4 cut(s) 376, 496, 695, 1039
NspV TTCGAA 1 cut(s) 686
OliI CACNNNNGTG 1 cut(s) 174
PaeR7I CTCGAG 2 cut(s) 540, 563
PagI TCATGA 1 cut(s) 1049
PciI ACATGT 1 cut(s) 492
PcsI WCGNNNNNNNCGW 1 cut(s) 712
PfeI GAWTC 1 cut(s) 84
PkrI GCNGC 2 cut(s) 570, 966
PmaCI CACGTG 1 cut(s) 788
PmlI CACGTG 1 cut(s) 788
Ppu21I YACGTR 2 cut(s) 663, 788
PscI ACATGT 1 cut(s) 492
PshAI GACNNNNGTC 1 cut(s) 243
Psp124BI GAGCTC 1 cut(s) 547
Psp6I CCWGG 1 cut(s) 195
PspCI CACGTG 1 cut(s) 788
PspGI CCWGG 1 cut(s) 195
PspN4I GGNNCC 5 cut(s) 181, 617, 629, 713, 909
PspOMI GGGCCC 1 cut(s) 711
PspPI GGNCC 6 cut(s) 179, 245, 616, 711, 712, 908
PspXI VCTCGAGB 2 cut(s) 540, 563
PsrI GAACNNNNNNTAC 2 cut(s) 329, 361
PstNI CAGNNNCTG 1 cut(s) 967
PsuI RGATCY 1 cut(s) 36
PvuII CAGCTG 1 cut(s) 964
RsaI GTAC 2 cut(s) 482, 497
RsaNI GTAC 2 cut(s) 481, 496
RseI CAYNNNNRTG 2 cut(s) 174, 462
SacI GAGCTC 1 cut(s) 547
SaqAI TTAA 1 cut(s) 41
SatI GCNGC 2 cut(s) 569, 965
Sau3AI GATC 2 cut(s) 36, 667
Sau96I GGNCC 6 cut(s) 179, 245, 616, 711, 712, 908
ScrFI CCNGG 2 cut(s) 197, 536
SduI GDGCHC 4 cut(s) 259, 547, 678, 715
SfaNI GCATC 1 cut(s) 105
SfcI CTRYAG 2 cut(s) 442, 993
Sfr274I CTCGAG 2 cut(s) 540, 563
SfuI TTCGAA 1 cut(s) 686
SinI GGWCC 2 cut(s) 179, 245
SlaI CTCGAG 2 cut(s) 540, 563
SmiMI CAYNNNNRTG 2 cut(s) 174, 462
SmlI CTYRAG 2 cut(s) 540, 563
SmoI CTYRAG 2 cut(s) 540, 563
Sse9I AATT 3 cut(s) 343, 510, 943
SsiI CCGC 3 cut(s) 569, 869, 886
SspI AATATT 1 cut(s) 646
SspMI CTAG 2 cut(s) 81, 506
SstI GAGCTC 1 cut(s) 547
StyD4I CCNGG 2 cut(s) 195, 534
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 2 cut(s) 364, 1047
TaiI ACGT 4 cut(s) 665, 790, 815, 920
TaqI TCGA 4 cut(s) 541, 564, 686, 849
TasI AATT 3 cut(s) 343, 510, 943
TatI WGTACW 2 cut(s) 480, 495
TauI GCSGC 1 cut(s) 571
TfiI GAWTC 1 cut(s) 84
Tru1I TTAA 1 cut(s) 41
Tru9I TTAA 1 cut(s) 41
TscAI CASTG 1 cut(s) 678
TseFI GTSAC 3 cut(s) 637, 808, 934
TseI GCWGC 1 cut(s) 964
Tsp45I GTSAC 3 cut(s) 637, 808, 934
TspDTI ATGAA 3 cut(s) 13, 127, 271
TspGWI ACGGA 1 cut(s) 668
TspRI CASTG 1 cut(s) 678
VneI GTGCAC 1 cut(s) 255
VpaK11BI GGWCC 2 cut(s) 179, 245
XbaI TCTAGA 1 cut(s) 80
XceI RCATGY 4 cut(s) 376, 496, 695, 1039
XcmI CCANNNNNNNNNTGG 1 cut(s) 193
XhoI CTCGAG 2 cut(s) 540, 563
XspI CTAG 2 cut(s) 81, 506
Zsp2I ATGCAT 1 cut(s) 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.