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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclI | AACGTT | 1 cut(s) | 658 |
| AgsI | TTSAA | 3 cut(s) | 317, 468, 672 |
| AluBI | AGCT | 1 cut(s) | 347 |
| AluI | AGCT | 1 cut(s) | 347 |
| AoxI | GGCC | 2 cut(s) | 25, 650 |
| AspS9I | GGNCC | 2 cut(s) | 25, 650 |
| AsuHPI | GGTGA | 1 cut(s) | 408 |
| BccI | CCATC | 3 cut(s) | 364, 558, 572 |
| BceAI | ACGGC | 1 cut(s) | 509 |
| BfaI | CTAG | 3 cut(s) | 29, 68, 344 |
| BmgT120I | GGNCC | 2 cut(s) | 25, 650 |
| BmsI | GCATC | 1 cut(s) | 480 |
| Bpu10I | CCTNAGC | 1 cut(s) | 86 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 412, 442 |
| Bse3DI | GCAATG | 3 cut(s) | 192, 258, 300 |
| BseGI | GGATG | 1 cut(s) | 367 |
| BseMI | GCAATG | 3 cut(s) | 192, 258, 300 |
| BseRI | GAGGAG | 1 cut(s) | 561 |
| BshFI | GGCC | 2 cut(s) | 27, 652 |
| BslFI | GGGAC | 1 cut(s) | 516 |
| BsmFI | GGGAC | 1 cut(s) | 516 |
| BsmI | GAATGC | 2 cut(s) | 325, 455 |
| BsnI | GGCC | 2 cut(s) | 27, 652 |
| BspANI | GGCC | 2 cut(s) | 27, 652 |
| BsrDI | GCAATG | 3 cut(s) | 192, 258, 300 |
| Bst6I | CTCTTC | 2 cut(s) | 635, 640 |
| BstDEI | CTNAG | 3 cut(s) | 86, 536, 614 |
| BstF5I | GGATG | 1 cut(s) | 367 |
| BstNSI | RCATGY | 9 cut(s) | 97, 111, 153, 177, 219, 243, 285, 325, 377 |
| BsuRI | GGCC | 2 cut(s) | 27, 652 |
| BtsCI | GGATG | 1 cut(s) | 367 |
| Cfr13I | GGNCC | 2 cut(s) | 25, 650 |
| CviJI | RGCY | 7 cut(s) | 27, 32, 54, 71, 90, 347, 652 |
| CviKI_1 | RGCY | 7 cut(s) | 27, 32, 54, 71, 90, 347, 652 |
| DdeI | CTNAG | 3 cut(s) | 86, 536, 614 |
| Eam1104I | CTCTTC | 2 cut(s) | 635, 640 |
| EarI | CTCTTC | 2 cut(s) | 635, 640 |
| EcoO109I | RGGNCCY | 2 cut(s) | 25, 650 |
| EcoT22I | ATGCAT | 3 cut(s) | 6, 99, 327 |
| FaqI | GGGAC | 1 cut(s) | 516 |
| FokI | GGATG | 1 cut(s) | 354 |
| FspBI | CTAG | 3 cut(s) | 29, 68, 344 |
| HaeIII | GGCC | 2 cut(s) | 27, 652 |
| HinfI | GANTC | 2 cut(s) | 529, 540 |
| HphI | GGTGA | 1 cut(s) | 408 |
| HpyCH4IV | ACGT | 1 cut(s) | 658 |
| HpyF3I | CTNAG | 3 cut(s) | 86, 536, 614 |
| HpySE526I | ACGT | 1 cut(s) | 658 |
| LweI | GCATC | 1 cut(s) | 480 |
| MaeI | CTAG | 3 cut(s) | 29, 68, 344 |
| MaeII | ACGT | 1 cut(s) | 658 |
| MboII | GAAGA | 3 cut(s) | 506, 652, 657 |
| MluCI | AATT | 2 cut(s) | 312, 667 |
| MlyI | GAGTC | 1 cut(s) | 538 |
| MmeI | TCCRAC | 1 cut(s) | 665 |
| MnlI | CCTC | 5 cut(s) | 75, 508, 582, 630, 641 |
| Mph1103I | ATGCAT | 3 cut(s) | 6, 99, 327 |
| MseI | TTAA | 1 cut(s) | 408 |
| MslI | CAYNNNNRTG | 7 cut(s) | 106, 148, 172, 214, 238, 280, 372 |
| Mva1269I | GAATGC | 2 cut(s) | 325, 455 |
| NsiI | ATGCAT | 3 cut(s) | 6, 99, 327 |
| NspI | RCATGY | 9 cut(s) | 97, 111, 153, 177, 219, 243, 285, 325, 377 |
| PctI | GAATGC | 2 cut(s) | 325, 455 |
| PfeI | GAWTC | 1 cut(s) | 540 |
| PleI | GAGTC | 1 cut(s) | 537 |
| PpsI | GAGTC | 1 cut(s) | 537 |
| Psp1406I | AACGTT | 1 cut(s) | 658 |
| PspPI | GGNCC | 2 cut(s) | 25, 650 |
| RseI | CAYNNNNRTG | 7 cut(s) | 106, 148, 172, 214, 238, 280, 372 |
| SaqAI | TTAA | 1 cut(s) | 408 |
| Sau96I | GGNCC | 2 cut(s) | 25, 650 |
| SchI | GAGTC | 1 cut(s) | 538 |
| SetI | ASST | 5 cut(s) | 67, 345, 349, 622, 661 |
| SfaNI | GCATC | 1 cut(s) | 480 |
| SmiMI | CAYNNNNRTG | 7 cut(s) | 106, 148, 172, 214, 238, 280, 372 |
| Sse9I | AATT | 2 cut(s) | 312, 667 |
| SspMI | CTAG | 3 cut(s) | 29, 68, 344 |
| TaiI | ACGT | 1 cut(s) | 661 |
| TasI | AATT | 2 cut(s) | 312, 667 |
| TfiI | GAWTC | 1 cut(s) | 540 |
| Tru1I | TTAA | 1 cut(s) | 408 |
| Tru9I | TTAA | 1 cut(s) | 408 |
| TspDTI | ATGAA | 8 cut(s) | 112, 178, 244, 286, 356, 448, 452, 651 |
| XceI | RCATGY | 9 cut(s) | 97, 111, 153, 177, 219, 243, 285, 325, 377 |
| XspI | CTAG | 3 cut(s) | 29, 68, 344 |
| Zsp2I | ATGCAT | 3 cut(s) | 6, 99, 327 |