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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AoxI | GGCC | 1 cut(s) | 25 |
| AspS9I | GGNCC | 1 cut(s) | 25 |
| BccI | CCATC | 2 cut(s) | 132, 235 |
| BfaI | CTAG | 2 cut(s) | 29, 68 |
| BmgT120I | GGNCC | 1 cut(s) | 25 |
| BmsI | GCATC | 1 cut(s) | 116 |
| Bpu10I | CCTNAGC | 1 cut(s) | 86 |
| Bse3DI | GCAATG | 1 cut(s) | 168 |
| BseMI | GCAATG | 1 cut(s) | 168 |
| BshFI | GGCC | 1 cut(s) | 27 |
| BsmI | GAATGC | 2 cut(s) | 97, 244 |
| BsnI | GGCC | 1 cut(s) | 27 |
| BspANI | GGCC | 1 cut(s) | 27 |
| BsrDI | GCAATG | 1 cut(s) | 168 |
| BstDEI | CTNAG | 1 cut(s) | 86 |
| BstNSI | RCATGY | 5 cut(s) | 97, 107, 153, 195, 244 |
| BsuRI | GGCC | 1 cut(s) | 27 |
| Cfr13I | GGNCC | 1 cut(s) | 25 |
| CviAII | CATG | 6 cut(s) | 56, 94, 104, 150, 192, 241 |
| CviJI | RGCY | 5 cut(s) | 27, 32, 54, 71, 90 |
| CviKI_1 | RGCY | 5 cut(s) | 27, 32, 54, 71, 90 |
| DdeI | CTNAG | 1 cut(s) | 86 |
| EcoO109I | RGGNCCY | 1 cut(s) | 25 |
| EcoT22I | ATGCAT | 3 cut(s) | 99, 109, 246 |
| FaeI | CATG | 6 cut(s) | 59, 97, 107, 153, 195, 244 |
| FaiI | YATR | 8 cut(s) | 15, 57, 95, 105, 151, 193, 242, 253 |
| FatI | CATG | 6 cut(s) | 55, 93, 103, 149, 191, 240 |
| FspBI | CTAG | 2 cut(s) | 29, 68 |
| HaeIII | GGCC | 1 cut(s) | 27 |
| Hin1II | CATG | 6 cut(s) | 59, 97, 107, 153, 195, 244 |
| Hpy188III | TCNNGA | 1 cut(s) | 128 |
| HpyAV | CCTTC | 2 cut(s) | 214, 231 |
| HpyCH4V | TGCA | 8 cut(s) | 4, 59, 97, 107, 153, 173, 195, 244 |
| HpyF3I | CTNAG | 1 cut(s) | 86 |
| Hsp92II | CATG | 6 cut(s) | 59, 97, 107, 153, 195, 244 |
| LweI | GCATC | 1 cut(s) | 116 |
| MaeI | CTAG | 2 cut(s) | 29, 68 |
| MnlI | CCTC | 1 cut(s) | 75 |
| Mph1103I | ATGCAT | 3 cut(s) | 99, 109, 246 |
| MslI | CAYNNNNRTG | 3 cut(s) | 102, 148, 190 |
| Mva1269I | GAATGC | 2 cut(s) | 97, 244 |
| NlaIII | CATG | 6 cut(s) | 59, 97, 107, 153, 195, 244 |
| NsiI | ATGCAT | 3 cut(s) | 99, 109, 246 |
| NspI | RCATGY | 5 cut(s) | 97, 107, 153, 195, 244 |
| PctI | GAATGC | 2 cut(s) | 97, 244 |
| PspPI | GGNCC | 1 cut(s) | 25 |
| RseI | CAYNNNNRTG | 3 cut(s) | 102, 148, 190 |
| Sau96I | GGNCC | 1 cut(s) | 25 |
| SetI | ASST | 2 cut(s) | 67, 223 |
| SfaNI | GCATC | 1 cut(s) | 116 |
| SgeI | CNNG | 8 cut(s) | 41, 68, 80, 106, 116, 140, 162, 204 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 102, 148, 190 |
| SspMI | CTAG | 2 cut(s) | 29, 68 |
| TspDTI | ATGAA | 3 cut(s) | 154, 196, 237 |
| XceI | RCATGY | 5 cut(s) | 97, 107, 153, 195, 244 |
| XspI | CTAG | 2 cut(s) | 29, 68 |
| Zsp2I | ATGCAT | 3 cut(s) | 99, 109, 246 |