pycom520g00190

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0001078
Physical Location & Seq
Forward (+)
176264 .. 177405
1142 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom520g00190.4

Sequence Viewer

Length: 969 bp
ATGCTTGGCGCCAAAGTGTTGGACGAAATTGAAGACCTAATTGAAGACCAAAGTGTTGGAACCTTGTTCCCTTTAGTTTTAGGACATTTAAACCTTTCCAATTTCCTTAGCCGTATCATTACTTATCATCACCACTTATCATATATCATAACCACATATCATATCACTTATCATAATCACTTATCACTTATCATCATCACTTATCATAACCATACACTTATCACTTATCATACTCATAATCATTCACACCTTGCAGCCACATGCATTCCCCTTTTGCACATGCTTTCCCAATTCATTAAAACATTGCACATGCACTCCCATCCTTTACCATTGCAGCCACATGCACTTCCCACTTCATCATTTCAGCCACACATTCACCCACATGCACTTTGCCTTTCATCATTGCACCACAAATCAGCACATTCACCTTCATTTCCAGATTGTCCCCTTGCACATGCAGCCACATATTCATTCACACCATCACACATTCATTCTCCATCCCTTGCCGTGAGTTCCCATCAGATTTCCAGCATATTCACATGCATTCCATCCTTTAAAACATTTGCACATGCATCTTCAATTAAATCAGCCACATGCATTCATAAAACCCTTGTGCCGTCCAAAACACATGCATTATCAGCACATGCATCTCCCATCACCATTCCAGATTTCCACCACCCTCCTAACTGTCATGTTCCCCCCATTTCACCTATAAATAAACCTTCATTGCAGCCACAAAGGAGGGGATCCATTCACCATAGAAATAAGGCCTTTGTAAACACCACCAAAAACCATCCCTTGGTGCCGTGCCTTAAGGCACCATTCCATCACTTCCCTATTAATCCAAACACCACTCCCCAAACCATTCACACCACCAAACCTCACCTTAGACCTTGTGCTACAACAACGAGGAAGAGAAGAGTGCCTAAACGTTCATACAATTCAAGTTTGAGTTGTTGGAATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

323

Amino Acids

36.11

Weight (kDa)

9.64

Isoelectric Point (pI)

62.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 8, 802, 817
AccB7I CCANNNNNTGG 1 cut(s) 799
AclI AACGTT 1 cut(s) 931
AclWI GGATC 2 cut(s) 741, 754
AcyI GRCGYC 1 cut(s) 9
AfiI CCNNNNNNNGG 1 cut(s) 799
AflII CTTAAG 1 cut(s) 812
AgsI TTSAA 4 cut(s) 32, 44, 579, 945
AlwI GGATC 2 cut(s) 741, 754
AoxI GGCC 1 cut(s) 768
ApeKI GCWGC 4 cut(s) 254, 334, 458, 730
AseI ATTAAT 1 cut(s) 840
AspLEI GCGC 1 cut(s) 11
AsuHPI GGTGA 7 cut(s) 122, 368, 417, 649, 699, 746, 875
BamHI GGATCC 1 cut(s) 746
BanI GGYRCC 3 cut(s) 8, 802, 817
BbsI GAAGAC 2 cut(s) 39, 51
BbvI GCAGC 4 cut(s) 266, 346, 470, 742
BccI CCATC 8 cut(s) 327, 487, 505, 525, 556, 662, 801, 834
BceAI ACGGC 4 cut(s) 96, 491, 601, 790
BfoI RGCGCY 1 cut(s) 12
BfrI CTTAAG 1 cut(s) 812
BisI GCNGC 4 cut(s) 255, 335, 459, 731
BlsI GCNGC 4 cut(s) 256, 336, 460, 732
BmiI GGNNCC 5 cut(s) 10, 61, 748, 804, 819
BmsI GCATC 2 cut(s) 581, 656
BpiI GAAGAC 2 cut(s) 39, 51
Bpu10I CCTNAGC 1 cut(s) 107
BsaHI GRCGYC 1 cut(s) 9
BsaJI CCNNGG 1 cut(s) 798
BsaXI ACNNNNNCTCC 2 cut(s) 299, 329
Bsc4I CCNNNNNNNGG 1 cut(s) 799
Bse3DI GCAATG 4 cut(s) 302, 329, 401, 725
BseDI CCNNGG 1 cut(s) 798
BseGI GGATG 4 cut(s) 319, 497, 548, 793
BseLI CCNNNNNNNGG 1 cut(s) 799
BseMI GCAATG 4 cut(s) 302, 329, 401, 725
BseXI GCAGC 4 cut(s) 266, 346, 470, 742
BshFI GGCC 1 cut(s) 770
BshNI GGYRCC 3 cut(s) 8, 802, 817
BslFI GGGAC 1 cut(s) 429
BslI CCNNNNNNNGG 1 cut(s) 799
BsmFI GGGAC 1 cut(s) 429
BsmI GAATGC 3 cut(s) 264, 543, 597
BsnI GGCC 1 cut(s) 770
Bsp143I GATC 1 cut(s) 746
BspANI GGCC 1 cut(s) 770
BspLI GGNNCC 5 cut(s) 10, 61, 748, 804, 819
BspPI GGATC 2 cut(s) 741, 754
BspT107I GGYRCC 3 cut(s) 8, 802, 817
BspTI CTTAAG 1 cut(s) 812
BsrDI GCAATG 4 cut(s) 302, 329, 401, 725
BssECI CCNNGG 1 cut(s) 798
BssMI GATC 1 cut(s) 746
BssNI GRCGYC 1 cut(s) 9
BssT1I CCWWGG 1 cut(s) 798
Bst4CI ACNGT 1 cut(s) 689
Bst6I CTCTTC 2 cut(s) 908, 913
BstACI GRCGYC 1 cut(s) 9
BstAFI CTTAAG 1 cut(s) 812
BstDEI CTNAG 2 cut(s) 107, 887
BstF5I GGATG 4 cut(s) 319, 497, 548, 793
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 1 cut(s) 749
BstMBI GATC 1 cut(s) 746
BstMWI GCNNNNNNNGC 2 cut(s) 458, 638
BstV1I GCAGC 4 cut(s) 266, 346, 470, 742
BstV2I GAAGAC 2 cut(s) 39, 51
BstX2I RGATCY 1 cut(s) 746
BstXI CCANNNNNNTGG 2 cut(s) 19, 56
BstYI RGATCY 1 cut(s) 746
BsuRI GGCC 1 cut(s) 770
BtsCI GGATG 4 cut(s) 319, 497, 548, 793
CfoI GCGC 1 cut(s) 11
CviJI RGCY 8 cut(s) 111, 257, 337, 367, 461, 590, 733, 770
CviKI_1 RGCY 8 cut(s) 111, 257, 337, 367, 461, 590, 733, 770
DdeI CTNAG 2 cut(s) 107, 887
DinI GGCGCC 1 cut(s) 10
DpnI GATC 1 cut(s) 748
DpnII GATC 1 cut(s) 746
DraI TTTAAA 2 cut(s) 90, 556
Eam1104I CTCTTC 2 cut(s) 908, 913
EarI CTCTTC 2 cut(s) 908, 913
Eco130I CCWWGG 1 cut(s) 798
Eco147I AGGCCT 1 cut(s) 770
EcoT14I CCWWGG 1 cut(s) 798
EcoT22I ATGCAT 6 cut(s) 266, 545, 574, 599, 634, 649
EgeI GGCGCC 1 cut(s) 10
EheI GGCGCC 1 cut(s) 10
ErhI CCWWGG 1 cut(s) 798
FaqI GGGAC 1 cut(s) 429
Fnu4HI GCNGC 4 cut(s) 255, 335, 459, 731
FokI GGATG 4 cut(s) 306, 484, 535, 780
Fsp4HI GCNGC 4 cut(s) 255, 335, 459, 731
GlaI GCGC 1 cut(s) 10
GluI GCNGC 4 cut(s) 255, 335, 459, 731
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 1 cut(s) 770
HhaI GCGC 1 cut(s) 11
Hin1I GRCGYC 1 cut(s) 9
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HphI GGTGA 7 cut(s) 122, 368, 417, 649, 699, 746, 875
Hpy166II GTNNAC 1 cut(s) 778
Hpy188I TCNGA 1 cut(s) 522
Hpy188III TCNNGA 2 cut(s) 437, 665
Hpy8I GTNNAC 1 cut(s) 778
HpyAV CCTTC 2 cut(s) 438, 732
HpyCH4III ACNGT 1 cut(s) 689
HpyCH4IV ACGT 1 cut(s) 931
HpyF10VI GCNNNNNNNGC 2 cut(s) 458, 638
HpyF3I CTNAG 2 cut(s) 107, 887
HpySE526I ACGT 1 cut(s) 931
Hsp92I GRCGYC 1 cut(s) 9
HspAI GCGC 1 cut(s) 9
KasI GGCGCC 1 cut(s) 8
Kzo9I GATC 1 cut(s) 746
LpnPI CCDG 3 cut(s) 450, 541, 678
Lsp1109I GCAGC 4 cut(s) 266, 346, 470, 742
LweI GCATC 2 cut(s) 581, 656
MaeII ACGT 1 cut(s) 931
MalI GATC 1 cut(s) 748
MboI GATC 1 cut(s) 746
MboII GAAGA 5 cut(s) 44, 56, 567, 925, 930
MflI RGATCY 1 cut(s) 746
MluCI AATT 6 cut(s) 27, 39, 100, 290, 579, 940
Mly113I GGCGCC 1 cut(s) 9
MmeI TCCRAC 2 cut(s) 37, 938
MnlI CCTC 4 cut(s) 690, 735, 891, 903
Mph1103I ATGCAT 6 cut(s) 266, 545, 574, 599, 634, 649
MseI TTAA 6 cut(s) 89, 297, 555, 582, 813, 840
MslI CAYNNNNRTG 1 cut(s) 381
MspCI CTTAAG 1 cut(s) 812
Mva1269I GAATGC 3 cut(s) 264, 543, 597
MwoI GCNNNNNNNGC 2 cut(s) 458, 638
NarI GGCGCC 1 cut(s) 9
NdeII GATC 1 cut(s) 746
NlaIV GGNNCC 5 cut(s) 10, 61, 748, 804, 819
NsiI ATGCAT 6 cut(s) 266, 545, 574, 599, 634, 649
PceI AGGCCT 1 cut(s) 770
PctI GAATGC 3 cut(s) 264, 543, 597
PflMI CCANNNNNTGG 1 cut(s) 799
PkrI GCNGC 4 cut(s) 256, 336, 460, 732
PluTI GGCGCC 1 cut(s) 12
PshBI ATTAAT 1 cut(s) 840
Psp1406I AACGTT 1 cut(s) 931
PspN4I GGNNCC 5 cut(s) 10, 61, 748, 804, 819
PsuI RGATCY 1 cut(s) 746
RseI CAYNNNNRTG 1 cut(s) 381
SaqAI TTAA 6 cut(s) 89, 297, 555, 582, 813, 840
SatI GCNGC 4 cut(s) 255, 335, 459, 731
Sau3AI GATC 1 cut(s) 746
SfaNI GCATC 2 cut(s) 581, 656
SfoI GGCGCC 1 cut(s) 10
SmiMI CAYNNNNRTG 1 cut(s) 381
SmlI CTYRAG 1 cut(s) 812
SmoI CTYRAG 1 cut(s) 812
Sse9I AATT 6 cut(s) 27, 39, 100, 290, 579, 940
SseBI AGGCCT 1 cut(s) 770
SspDI GGCGCC 1 cut(s) 8
StuI AGGCCT 1 cut(s) 770
StyI CCWWGG 1 cut(s) 798
TaaI ACNGT 1 cut(s) 689
TaiI ACGT 1 cut(s) 934
TasI AATT 6 cut(s) 27, 39, 100, 290, 579, 940
Tru1I TTAA 6 cut(s) 89, 297, 555, 582, 813, 840
Tru9I TTAA 6 cut(s) 89, 297, 555, 582, 813, 840
TseI GCWGC 4 cut(s) 254, 334, 458, 730
TspDTI ATGAA 9 cut(s) 283, 345, 387, 420, 459, 479, 590, 714, 924
Van91I CCANNNNNTGG 1 cut(s) 799
Vha464I CTTAAG 1 cut(s) 812
VspI ATTAAT 1 cut(s) 840
Zsp2I ATGCAT 6 cut(s) 266, 545, 574, 599, 634, 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.