pycom436g00200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_436
OG0001078
Physical Location & Seq
Forward (+)
281746 .. 282733
988 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom436g00200.1

Sequence Viewer

Length: 813 bp
ATGGCTTGTTCTCGTGCATTTACGTTATGTTTCTTTAGTTATTTTAGTCCTTTAAGCTATTTTCGTGTGTTTCTTATGCTTGGCGCCAAAATGTTGGACGAAATTGAAGACCAAAGTGTTGGAACTTTGTTCCCTTTAGTTTTAGGACATTTAAACCTTTCCAATTCCCTTATGCCGTGCATCTCCACCTTTCTCCCAAAATCATACACATGCTCCCATCACATATCATATCACTTATCATTCATCACTTATCATTCATCACATATCATATCACTTATCATTCACCACTTATCATTCATCAAACACATGCATTCACATGTTTTCACAATCTGCCATCATTACAACCAAAACCGTGCACATGCTATCACCATGCATTCACATGCACTTGTTCCTCCATCATTCCATCCTTTAAAGCATTGCACATGCACTTTAAACATTCACCCACATGCATTTCCACCCACATGCTATCCCATTTCAGATTGTCATTTCCCCTTTAATCAACACATGCATCCCCTCACCAAGAAATCATTCATTCCACATATTTCACATGCACTCTTGCACATTCACCTTAATTTTCAGATTATTCCTTCATCATTGCACCACACATTCATTGCACATCACATGCAACCTAGCTGTCATGTTCCCCCCATTTCACCTATAAATACTCATTCATTCATTCTCATTCATACACAATTCATTTCACAACAGAAATTGGTCCCTTGGGGCCATGCCCTTCACAAAAGTCCATCCATTCCATTCATACACTTCATCCATTGCAGAAATGTAGACCATAAACCCCCCTTTGTGCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

271

Amino Acids

31.1

Weight (kDa)

8.9

Isoelectric Point (pI)

64.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 83
AccI GTMKAC 1 cut(s) 786
AcyI GRCGYC 1 cut(s) 84
AflIII ACRYGT 1 cut(s) 316
AgsI TTSAA 1 cut(s) 107
AjuI GAANNNNNNNTTGG 2 cut(s) 512, 544
AluBI AGCT 2 cut(s) 57, 633
AluI AGCT 2 cut(s) 57, 633
Alw21I GWGCWC 1 cut(s) 358
Alw44I GTGCAC 1 cut(s) 354
AoxI GGCC 1 cut(s) 724
ApaLI GTGCAC 1 cut(s) 354
Asp700I GAANNNNTTC 1 cut(s) 527
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 2 cut(s) 715, 724
AsuHPI GGTGA 6 cut(s) 275, 358, 431, 508, 557, 645
AvaII GGWCC 1 cut(s) 715
BaeGI GKGCMC 1 cut(s) 358
BanI GGYRCC 1 cut(s) 83
BauI CACGAG 1 cut(s) 12
BbsI GAAGAC 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 358
BccI CCATC 5 cut(s) 225, 342, 403, 411, 754
BceAI ACGGC 2 cut(s) 160, 793
BfaI CTAG 1 cut(s) 630
BfoI RGCGCY 1 cut(s) 87
Bme18I GGWCC 1 cut(s) 715
BmgT120I GGNCC 2 cut(s) 715, 724
BmiI GGNNCC 3 cut(s) 85, 717, 725
BmsI GCATC 2 cut(s) 189, 517
BpiI GAAGAC 1 cut(s) 114
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 1 cut(s) 719
BsaXI ACNNNNNCTCC 2 cut(s) 197, 227
Bse3DI GCAATG 4 cut(s) 415, 593, 609, 772
BseDI CCNNGG 1 cut(s) 719
BseGI GGATG 4 cut(s) 403, 508, 746, 768
BseMI GCAATG 4 cut(s) 415, 593, 609, 772
BseSI GKGCMC 1 cut(s) 358
BshFI GGCC 1 cut(s) 726
BshNI GGYRCC 1 cut(s) 83
BsiHKAI GWGCWC 1 cut(s) 358
BslFI GGGAC 1 cut(s) 701
BsmFI GGGAC 1 cut(s) 701
BsmI GAATGC 2 cut(s) 310, 373
BsnI GGCC 1 cut(s) 726
Bsp1286I GDGCHC 1 cut(s) 358
BspANI GGCC 1 cut(s) 726
BspLI GGNNCC 3 cut(s) 85, 717, 725
BspT107I GGYRCC 1 cut(s) 83
BsrDI GCAATG 4 cut(s) 415, 593, 609, 772
BssECI CCNNGG 1 cut(s) 719
BssNI GRCGYC 1 cut(s) 84
BssSI CACGAG 1 cut(s) 12
BssT1I CCWWGG 1 cut(s) 719
Bst2BI CACGAG 1 cut(s) 12
Bst4CI ACNGT 1 cut(s) 353
BstACI GRCGYC 1 cut(s) 84
BstF5I GGATG 4 cut(s) 403, 508, 746, 768
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 1 cut(s) 86
BstSLI GKGCMC 1 cut(s) 358
BstV2I GAAGAC 1 cut(s) 114
BstXI CCANNNNNNTGG 2 cut(s) 94, 119
BsuRI GGCC 1 cut(s) 726
BtsCI GGATG 4 cut(s) 403, 508, 746, 768
CfoI GCGC 1 cut(s) 86
Cfr13I GGNCC 2 cut(s) 715, 724
CviJI RGCY 4 cut(s) 5, 57, 633, 726
CviKI_1 RGCY 4 cut(s) 5, 57, 633, 726
DinI GGCGCC 1 cut(s) 85
DraI TTTAAA 3 cut(s) 153, 411, 432
Eco130I CCWWGG 1 cut(s) 719
Eco47I GGWCC 1 cut(s) 715
EcoT14I CCWWGG 1 cut(s) 719
EcoT22I ATGCAT 4 cut(s) 312, 375, 451, 510
EgeI GGCGCC 1 cut(s) 85
EheI GGCGCC 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 719
FaqI GGGAC 1 cut(s) 701
FblI GTMKAC 1 cut(s) 786
FokI GGATG 4 cut(s) 390, 495, 733, 755
FspBI CTAG 1 cut(s) 630
GlaI GCGC 1 cut(s) 85
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 1 cut(s) 726
HhaI GCGC 1 cut(s) 86
Hin1I GRCGYC 1 cut(s) 84
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HphI GGTGA 6 cut(s) 275, 358, 431, 508, 557, 645
Hpy166II GTNNAC 2 cut(s) 356, 787
Hpy188I TCNGA 2 cut(s) 478, 579
Hpy8I GTNNAC 2 cut(s) 356, 787
HpyAV CCTTC 2 cut(s) 597, 743
HpyCH4III ACNGT 1 cut(s) 353
HpyCH4IV ACGT 1 cut(s) 23
HpySE526I ACGT 1 cut(s) 23
Hsp92I GRCGYC 1 cut(s) 84
HspAI GCGC 1 cut(s) 84
KasI GGCGCC 1 cut(s) 83
LmnI GCTCC 1 cut(s) 218
LweI GCATC 2 cut(s) 189, 517
MaeI CTAG 1 cut(s) 630
MaeII ACGT 1 cut(s) 23
MboII GAAGA 1 cut(s) 119
MhlI GDGCHC 1 cut(s) 358
MluCI AATT 5 cut(s) 102, 163, 571, 692, 710
Mly113I GGCGCC 1 cut(s) 84
MmeI TCCRAC 2 cut(s) 75, 100
MnlI CCTC 2 cut(s) 402, 524
Mph1103I ATGCAT 4 cut(s) 312, 375, 451, 510
MroXI GAANNNNTTC 1 cut(s) 527
MseI TTAA 6 cut(s) 53, 152, 410, 431, 495, 570
MslI CAYNNNNRTG 5 cut(s) 208, 315, 378, 444, 460
Mva1269I GAATGC 2 cut(s) 310, 373
NarI GGCGCC 1 cut(s) 84
NlaIV GGNNCC 3 cut(s) 85, 717, 725
NsiI ATGCAT 4 cut(s) 312, 375, 451, 510
PciI ACATGT 1 cut(s) 316
PctI GAATGC 2 cut(s) 310, 373
PdmI GAANNNNTTC 1 cut(s) 527
PluTI GGCGCC 1 cut(s) 87
PscI ACATGT 1 cut(s) 316
PspN4I GGNNCC 3 cut(s) 85, 717, 725
PspPI GGNCC 2 cut(s) 715, 724
RseI CAYNNNNRTG 5 cut(s) 208, 315, 378, 444, 460
SaqAI TTAA 6 cut(s) 53, 152, 410, 431, 495, 570
Sau96I GGNCC 2 cut(s) 715, 724
SduI GDGCHC 1 cut(s) 358
SetI ASST 8 cut(s) 26, 59, 159, 191, 570, 631, 635, 658
SfaNI GCATC 2 cut(s) 189, 517
SfoI GGCGCC 1 cut(s) 85
SinI GGWCC 1 cut(s) 715
SmiMI CAYNNNNRTG 5 cut(s) 208, 315, 378, 444, 460
Sse9I AATT 5 cut(s) 102, 163, 571, 692, 710
SspDI GGCGCC 1 cut(s) 83
SspMI CTAG 1 cut(s) 630
StyI CCWWGG 1 cut(s) 719
TaaI ACNGT 1 cut(s) 353
TaiI ACGT 1 cut(s) 26
TasI AATT 5 cut(s) 102, 163, 571, 692, 710
Tru1I TTAA 6 cut(s) 53, 152, 410, 431, 495, 570
Tru9I TTAA 6 cut(s) 53, 152, 410, 431, 495, 570
VneI GTGCAC 1 cut(s) 354
VpaK11BI GGWCC 1 cut(s) 715
XmiI GTMKAC 1 cut(s) 786
XmnI GAANNNNTTC 1 cut(s) 527
XspI CTAG 1 cut(s) 630
Zsp2I ATGCAT 4 cut(s) 312, 375, 451, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.