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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 703 |
| AclWI | GGATC | 2 cut(s) | 529, 542 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 100 |
| AleI | CACNNNNGTG | 1 cut(s) | 183 |
| AluBI | AGCT | 3 cut(s) | 344, 470, 552 |
| AluI | AGCT | 3 cut(s) | 344, 470, 552 |
| AlwI | GGATC | 2 cut(s) | 529, 542 |
| AoxI | GGCC | 1 cut(s) | 102 |
| ApeKI | GCWGC | 1 cut(s) | 746 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 184, 216 |
| AseI | ATTAAT | 1 cut(s) | 390 |
| AspS9I | GGNCC | 2 cut(s) | 22, 103 |
| AsuHPI | GGTGA | 2 cut(s) | 152, 170 |
| AvaII | GGWCC | 1 cut(s) | 22 |
| BamHI | GGATCC | 1 cut(s) | 534 |
| BbvI | GCAGC | 1 cut(s) | 758 |
| BccI | CCATC | 2 cut(s) | 392, 776 |
| BceAI | ACGGC | 4 cut(s) | 83, 173, 303, 513 |
| BfaI | CTAG | 2 cut(s) | 45, 558 |
| BisI | GCNGC | 2 cut(s) | 703, 747 |
| BlsI | GCNGC | 2 cut(s) | 704, 748 |
| Bme18I | GGWCC | 1 cut(s) | 22 |
| BmgT120I | GGNCC | 2 cut(s) | 22, 103 |
| BmiI | GGNNCC | 2 cut(s) | 23, 536 |
| BmsI | GCATC | 2 cut(s) | 231, 666 |
| BsaJI | CCNNGG | 3 cut(s) | 99, 319, 529 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 100 |
| Bse3DI | GCAATG | 3 cut(s) | 523, 652, 741 |
| BseDI | CCNNGG | 3 cut(s) | 99, 319, 529 |
| BseGI | GGATG | 1 cut(s) | 768 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 100 |
| BseMI | GCAATG | 3 cut(s) | 523, 652, 741 |
| BseRI | GAGGAG | 1 cut(s) | 727 |
| BseXI | GCAGC | 1 cut(s) | 758 |
| BsgI | GTGCAG | 1 cut(s) | 746 |
| BshFI | GGCC | 1 cut(s) | 104 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 100 |
| BsmI | GAATGC | 3 cut(s) | 367, 424, 641 |
| BsnI | GGCC | 1 cut(s) | 104 |
| Bsp143I | GATC | 1 cut(s) | 534 |
| BspACI | CCGC | 1 cut(s) | 703 |
| BspANI | GGCC | 1 cut(s) | 104 |
| BspLI | GGNNCC | 2 cut(s) | 23, 536 |
| BspPI | GGATC | 2 cut(s) | 529, 542 |
| BsrDI | GCAATG | 3 cut(s) | 523, 652, 741 |
| BssECI | CCNNGG | 3 cut(s) | 99, 319, 529 |
| BssMI | GATC | 1 cut(s) | 534 |
| Bst4CI | ACNGT | 1 cut(s) | 378 |
| BstC8I | GCNNGC | 1 cut(s) | 590 |
| BstDSI | CCRYGG | 3 cut(s) | 99, 319, 529 |
| BstF5I | GGATG | 1 cut(s) | 768 |
| BstKTI | GATC | 1 cut(s) | 537 |
| BstMBI | GATC | 1 cut(s) | 534 |
| BstNSI | RCATGY | 9 cut(s) | 154, 222, 283, 367, 424, 460, 502, 588, 592 |
| BstV1I | GCAGC | 1 cut(s) | 758 |
| BstX2I | RGATCY | 1 cut(s) | 534 |
| BstYI | RGATCY | 1 cut(s) | 534 |
| BsuRI | GGCC | 1 cut(s) | 104 |
| BtgI | CCRYGG | 3 cut(s) | 99, 319, 529 |
| BtsCI | GGATG | 1 cut(s) | 768 |
| Cac8I | GCNNGC | 1 cut(s) | 590 |
| Cfr13I | GGNCC | 2 cut(s) | 22, 103 |
| DpnI | GATC | 1 cut(s) | 536 |
| DpnII | GATC | 1 cut(s) | 534 |
| Eco47I | GGWCC | 1 cut(s) | 22 |
| EcoT22I | ATGCAT | 5 cut(s) | 224, 369, 426, 504, 590 |
| Fnu4HI | GCNGC | 2 cut(s) | 703, 747 |
| FokI | GGATG | 1 cut(s) | 755 |
| Fsp4HI | GCNGC | 2 cut(s) | 703, 747 |
| FspBI | CTAG | 2 cut(s) | 45, 558 |
| GluI | GCNGC | 2 cut(s) | 703, 747 |
| HaeIII | GGCC | 1 cut(s) | 104 |
| HindIII | AAGCTT | 1 cut(s) | 468 |
| HinfI | GANTC | 1 cut(s) | 169 |
| HphI | GGTGA | 2 cut(s) | 152, 170 |
| HpyAV | CCTTC | 1 cut(s) | 723 |
| HpyCH4III | ACNGT | 1 cut(s) | 378 |
| Kzo9I | GATC | 1 cut(s) | 534 |
| LmnI | GCTCC | 1 cut(s) | 11 |
| LpnPI | CCDG | 3 cut(s) | 354, 562, 592 |
| Lsp1109I | GCAGC | 1 cut(s) | 758 |
| LweI | GCATC | 2 cut(s) | 231, 666 |
| MaeI | CTAG | 2 cut(s) | 45, 558 |
| MaeIII | GTNAC | 1 cut(s) | 190 |
| MalI | GATC | 1 cut(s) | 536 |
| MboI | GATC | 1 cut(s) | 534 |
| MflI | RGATCY | 1 cut(s) | 534 |
| MluCI | AATT | 5 cut(s) | 15, 51, 62, 240, 391 |
| MmeI | TCCRAC | 1 cut(s) | 781 |
| MnlI | CCTC | 3 cut(s) | 625, 748, 783 |
| Mph1103I | ATGCAT | 5 cut(s) | 224, 369, 426, 504, 590 |
| MseI | TTAA | 5 cut(s) | 38, 140, 390, 473, 594 |
| MslI | CAYNNNNRTG | 6 cut(s) | 149, 183, 298, 528, 587, 722 |
| Mva1269I | GAATGC | 3 cut(s) | 367, 424, 641 |
| NdeII | GATC | 1 cut(s) | 534 |
| NlaIV | GGNNCC | 2 cut(s) | 23, 536 |
| NmuCI | GTSAC | 1 cut(s) | 190 |
| NsiI | ATGCAT | 5 cut(s) | 224, 369, 426, 504, 590 |
| NspI | RCATGY | 9 cut(s) | 154, 222, 283, 367, 424, 460, 502, 588, 592 |
| OliI | CACNNNNGTG | 1 cut(s) | 183 |
| PaeI | GCATGC | 1 cut(s) | 592 |
| PctI | GAATGC | 3 cut(s) | 367, 424, 641 |
| PfeI | GAWTC | 1 cut(s) | 169 |
| PkrI | GCNGC | 2 cut(s) | 704, 748 |
| PshBI | ATTAAT | 1 cut(s) | 390 |
| PspN4I | GGNNCC | 2 cut(s) | 23, 536 |
| PspPI | GGNCC | 2 cut(s) | 22, 103 |
| PsuI | RGATCY | 1 cut(s) | 534 |
| RseI | CAYNNNNRTG | 6 cut(s) | 149, 183, 298, 528, 587, 722 |
| SaqAI | TTAA | 5 cut(s) | 38, 140, 390, 473, 594 |
| SatI | GCNGC | 2 cut(s) | 703, 747 |
| Sau3AI | GATC | 1 cut(s) | 534 |
| Sau96I | GGNCC | 2 cut(s) | 22, 103 |
| SetI | ASST | 5 cut(s) | 183, 346, 472, 554, 617 |
| SfaNI | GCATC | 2 cut(s) | 231, 666 |
| SinI | GGWCC | 1 cut(s) | 22 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 149, 183, 298, 528, 587, 722 |
| SphI | GCATGC | 1 cut(s) | 592 |
| Sse9I | AATT | 5 cut(s) | 15, 51, 62, 240, 391 |
| SsiI | CCGC | 1 cut(s) | 703 |
| SspMI | CTAG | 2 cut(s) | 45, 558 |
| TaaI | ACNGT | 1 cut(s) | 378 |
| TasI | AATT | 5 cut(s) | 15, 51, 62, 240, 391 |
| TauI | GCSGC | 1 cut(s) | 705 |
| TfiI | GAWTC | 1 cut(s) | 169 |
| Tru1I | TTAA | 5 cut(s) | 38, 140, 390, 473, 594 |
| Tru9I | TTAA | 5 cut(s) | 38, 140, 390, 473, 594 |
| TseFI | GTSAC | 1 cut(s) | 190 |
| TseI | GCWGC | 1 cut(s) | 746 |
| Tsp45I | GTSAC | 1 cut(s) | 190 |
| TspDTI | ATGAA | 5 cut(s) | 19, 90, 282, 317, 656 |
| VpaK11BI | GGWCC | 1 cut(s) | 22 |
| VspI | ATTAAT | 1 cut(s) | 390 |
| XceI | RCATGY | 9 cut(s) | 154, 222, 283, 367, 424, 460, 502, 588, 592 |
| XspI | CTAG | 2 cut(s) | 45, 558 |
| Zsp2I | ATGCAT | 5 cut(s) | 224, 369, 426, 504, 590 |