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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 473 |
| AcsI | RAATTY | 2 cut(s) | 237, 749 |
| AfaI | GTAC | 2 cut(s) | 552, 611 |
| AgsI | TTSAA | 5 cut(s) | 145, 242, 522, 559, 693 |
| AluBI | AGCT | 2 cut(s) | 272, 459 |
| AluI | AGCT | 2 cut(s) | 272, 459 |
| AoxI | GGCC | 1 cut(s) | 25 |
| ApoI | RAATTY | 2 cut(s) | 237, 749 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 668, 700 |
| AspS9I | GGNCC | 1 cut(s) | 25 |
| AsuHPI | GGTGA | 1 cut(s) | 333 |
| BccI | CCATC | 1 cut(s) | 289 |
| BfaI | CTAG | 3 cut(s) | 29, 68, 269 |
| BmgT120I | GGNCC | 1 cut(s) | 25 |
| BmsI | GCATC | 1 cut(s) | 239 |
| Bpu10I | CCTNAGC | 1 cut(s) | 86 |
| BpuEI | CTTGAG | 1 cut(s) | 475 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 337, 367 |
| Bse3DI | GCAATG | 3 cut(s) | 183, 225, 447 |
| BseGI | GGATG | 4 cut(s) | 292, 432, 492, 535 |
| BseMI | GCAATG | 3 cut(s) | 183, 225, 447 |
| BseRI | GAGGAG | 1 cut(s) | 479 |
| BshFI | GGCC | 1 cut(s) | 27 |
| BsmI | GAATGC | 2 cut(s) | 250, 380 |
| BsnI | GGCC | 1 cut(s) | 27 |
| Bsp1407I | TGTACA | 1 cut(s) | 550 |
| BspANI | GGCC | 1 cut(s) | 27 |
| BsrDI | GCAATG | 3 cut(s) | 183, 225, 447 |
| BsrGI | TGTACA | 1 cut(s) | 550 |
| BstAUI | TGTACA | 1 cut(s) | 550 |
| BstDEI | CTNAG | 3 cut(s) | 86, 541, 802 |
| BstF5I | GGATG | 4 cut(s) | 292, 432, 492, 535 |
| BstNSI | RCATGY | 5 cut(s) | 97, 153, 210, 250, 302 |
| BsuRI | GGCC | 1 cut(s) | 27 |
| BtsCI | GGATG | 4 cut(s) | 292, 432, 492, 535 |
| Cfr13I | GGNCC | 1 cut(s) | 25 |
| Csp6I | GTAC | 2 cut(s) | 551, 610 |
| CviJI | RGCY | 8 cut(s) | 27, 32, 54, 71, 90, 272, 459, 633 |
| CviKI_1 | RGCY | 8 cut(s) | 27, 32, 54, 71, 90, 272, 459, 633 |
| CviQI | GTAC | 2 cut(s) | 551, 610 |
| DdeI | CTNAG | 3 cut(s) | 86, 541, 802 |
| EcoO109I | RGGNCCY | 1 cut(s) | 25 |
| EcoRI | GAATTC | 1 cut(s) | 749 |
| EcoT22I | ATGCAT | 4 cut(s) | 99, 155, 252, 382 |
| FokI | GGATG | 4 cut(s) | 279, 419, 479, 522 |
| FspBI | CTAG | 3 cut(s) | 29, 68, 269 |
| HaeIII | GGCC | 1 cut(s) | 27 |
| HinfI | GANTC | 1 cut(s) | 595 |
| HphI | GGTGA | 1 cut(s) | 333 |
| Hpy166II | GTNNAC | 1 cut(s) | 718 |
| Hpy188I | TCNGA | 2 cut(s) | 748, 788 |
| Hpy8I | GTNNAC | 1 cut(s) | 718 |
| HpyAV | CCTTC | 2 cut(s) | 116, 172 |
| HpyF3I | CTNAG | 3 cut(s) | 86, 541, 802 |
| LweI | GCATC | 1 cut(s) | 239 |
| MaeI | CTAG | 3 cut(s) | 29, 68, 269 |
| MboII | GAAGA | 1 cut(s) | 432 |
| MluCI | AATT | 7 cut(s) | 140, 237, 505, 554, 742, 749, 793 |
| MmeI | TCCRAC | 1 cut(s) | 552 |
| MnlI | CCTC | 5 cut(s) | 75, 455, 500, 610, 636 |
| Mph1103I | ATGCAT | 4 cut(s) | 99, 155, 252, 382 |
| MseI | TTAA | 5 cut(s) | 333, 665, 741, 759, 792 |
| MslI | CAYNNNNRTG | 2 cut(s) | 205, 297 |
| Mva1269I | GAATGC | 2 cut(s) | 250, 380 |
| NmeAIII | GCCGAG | 1 cut(s) | 609 |
| NsiI | ATGCAT | 4 cut(s) | 99, 155, 252, 382 |
| NspI | RCATGY | 5 cut(s) | 97, 153, 210, 250, 302 |
| PctI | GAATGC | 2 cut(s) | 250, 380 |
| PfeI | GAWTC | 1 cut(s) | 595 |
| PsiI | TTATAA | 1 cut(s) | 473 |
| PspPI | GGNCC | 1 cut(s) | 25 |
| RsaI | GTAC | 2 cut(s) | 552, 611 |
| RsaNI | GTAC | 2 cut(s) | 551, 610 |
| RseI | CAYNNNNRTG | 2 cut(s) | 205, 297 |
| SaqAI | TTAA | 5 cut(s) | 333, 665, 741, 759, 792 |
| Sau96I | GGNCC | 1 cut(s) | 25 |
| SfaNI | GCATC | 1 cut(s) | 239 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 205, 297 |
| SmlI | CTYRAG | 1 cut(s) | 454 |
| SmoI | CTYRAG | 1 cut(s) | 454 |
| Sse9I | AATT | 7 cut(s) | 140, 237, 505, 554, 742, 749, 793 |
| SspMI | CTAG | 3 cut(s) | 29, 68, 269 |
| TaqI | TCGA | 1 cut(s) | 675 |
| TasI | AATT | 7 cut(s) | 140, 237, 505, 554, 742, 749, 793 |
| TatI | WGTACW | 1 cut(s) | 550 |
| TfiI | GAWTC | 1 cut(s) | 595 |
| Tru1I | TTAA | 5 cut(s) | 333, 665, 741, 759, 792 |
| Tru9I | TTAA | 5 cut(s) | 333, 665, 741, 759, 792 |
| XapI | RAATTY | 2 cut(s) | 237, 749 |
| XceI | RCATGY | 5 cut(s) | 97, 153, 210, 250, 302 |
| XspI | CTAG | 3 cut(s) | 29, 68, 269 |
| Zsp2I | ATGCAT | 4 cut(s) | 99, 155, 252, 382 |