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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 334 |
| AcuI | CTGAAG | 1 cut(s) | 192 |
| AfaI | GTAC | 1 cut(s) | 348 |
| AgsI | TTSAA | 2 cut(s) | 195, 223 |
| AluBI | AGCT | 2 cut(s) | 206, 249 |
| AluI | AGCT | 2 cut(s) | 206, 249 |
| ApeKI | GCWGC | 1 cut(s) | 230 |
| ApoI | RAATTY | 1 cut(s) | 334 |
| AseI | ATTAAT | 1 cut(s) | 393 |
| AsuHPI | GGTGA | 2 cut(s) | 261, 411 |
| BbvI | GCAGC | 1 cut(s) | 242 |
| BccI | CCATC | 3 cut(s) | 90, 413, 451 |
| BceAI | ACGGC | 1 cut(s) | 362 |
| BfaI | CTAG | 2 cut(s) | 203, 246 |
| BisI | GCNGC | 1 cut(s) | 231 |
| BlsI | GCNGC | 1 cut(s) | 232 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 6, 36, 116, 146, 226, 256 |
| Bse3DI | GCAATG | 1 cut(s) | 59 |
| BseGI | GGATG | 2 cut(s) | 338, 405 |
| BseMI | GCAATG | 1 cut(s) | 59 |
| BseRI | GAGGAG | 1 cut(s) | 311 |
| BseXI | GCAGC | 1 cut(s) | 242 |
| BsmI | GAATGC | 2 cut(s) | 101, 154 |
| Bsp1407I | TGTACA | 1 cut(s) | 346 |
| BsrDI | GCAATG | 1 cut(s) | 59 |
| BsrGI | TGTACA | 1 cut(s) | 346 |
| BstAUI | TGTACA | 1 cut(s) | 346 |
| BstC8I | GCNNGC | 1 cut(s) | 285 |
| BstDEI | CTNAG | 1 cut(s) | 456 |
| BstF5I | GGATG | 2 cut(s) | 338, 405 |
| BstNSI | RCATGY | 6 cut(s) | 20, 130, 154, 180, 230, 287 |
| BstV1I | GCAGC | 1 cut(s) | 242 |
| BtsCI | GGATG | 2 cut(s) | 338, 405 |
| Cac8I | GCNNGC | 1 cut(s) | 285 |
| Csp6I | GTAC | 1 cut(s) | 347 |
| CviAII | CATG | 7 cut(s) | 17, 127, 151, 177, 227, 254, 284 |
| CviJI | RGCY | 7 cut(s) | 45, 76, 173, 206, 212, 233, 249 |
| CviKI_1 | RGCY | 7 cut(s) | 45, 76, 173, 206, 212, 233, 249 |
| CviQI | GTAC | 1 cut(s) | 347 |
| DdeI | CTNAG | 1 cut(s) | 456 |
| DraI | TTTAAA | 1 cut(s) | 144 |
| Eco57I | CTGAAG | 1 cut(s) | 192 |
| EcoT22I | ATGCAT | 3 cut(s) | 6, 156, 289 |
| FaeI | CATG | 7 cut(s) | 20, 130, 154, 180, 230, 257, 287 |
| FatI | CATG | 7 cut(s) | 16, 126, 150, 176, 226, 253, 283 |
| Fnu4HI | GCNGC | 1 cut(s) | 231 |
| FokI | GGATG | 2 cut(s) | 325, 392 |
| Fsp4HI | GCNGC | 1 cut(s) | 231 |
| FspBI | CTAG | 2 cut(s) | 203, 246 |
| GluI | GCNGC | 1 cut(s) | 231 |
| Hin1II | CATG | 7 cut(s) | 20, 130, 154, 180, 230, 257, 287 |
| HphI | GGTGA | 2 cut(s) | 261, 411 |
| Hpy188I | TCNGA | 2 cut(s) | 96, 332 |
| HpyAV | CCTTC | 2 cut(s) | 170, 419 |
| HpyCH4V | TGCA | 9 cut(s) | 4, 20, 64, 101, 130, 154, 180, 230, 287 |
| HpyF3I | CTNAG | 1 cut(s) | 456 |
| Hsp92II | CATG | 7 cut(s) | 20, 130, 154, 180, 230, 257, 287 |
| Lsp1109I | GCAGC | 1 cut(s) | 242 |
| MaeI | CTAG | 2 cut(s) | 203, 246 |
| MluCI | AATT | 1 cut(s) | 334 |
| MmeI | TCCRAC | 1 cut(s) | 139 |
| MnlI | CCTC | 2 cut(s) | 252, 332 |
| Mph1103I | ATGCAT | 3 cut(s) | 6, 156, 289 |
| MseI | TTAA | 3 cut(s) | 33, 143, 393 |
| MslI | CAYNNNNRTG | 1 cut(s) | 15 |
| Mva1269I | GAATGC | 2 cut(s) | 101, 154 |
| NlaIII | CATG | 7 cut(s) | 20, 130, 154, 180, 230, 257, 287 |
| NsiI | ATGCAT | 3 cut(s) | 6, 156, 289 |
| NspI | RCATGY | 6 cut(s) | 20, 130, 154, 180, 230, 287 |
| PaeI | GCATGC | 1 cut(s) | 287 |
| PctI | GAATGC | 2 cut(s) | 101, 154 |
| PkrI | GCNGC | 1 cut(s) | 232 |
| PshBI | ATTAAT | 1 cut(s) | 393 |
| RsaI | GTAC | 1 cut(s) | 348 |
| RsaNI | GTAC | 1 cut(s) | 347 |
| RseI | CAYNNNNRTG | 1 cut(s) | 15 |
| SaqAI | TTAA | 3 cut(s) | 33, 143, 393 |
| SatI | GCNGC | 1 cut(s) | 231 |
| SetI | ASST | 4 cut(s) | 204, 208, 251, 274 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 15 |
| SphI | GCATGC | 1 cut(s) | 287 |
| Sse9I | AATT | 1 cut(s) | 334 |
| SspMI | CTAG | 2 cut(s) | 203, 246 |
| TasI | AATT | 1 cut(s) | 334 |
| TatI | WGTACW | 1 cut(s) | 346 |
| Tru1I | TTAA | 3 cut(s) | 33, 143, 393 |
| Tru9I | TTAA | 3 cut(s) | 33, 143, 393 |
| TseI | GCWGC | 1 cut(s) | 230 |
| TspDTI | ATGAA | 4 cut(s) | 152, 291, 302, 327 |
| VspI | ATTAAT | 1 cut(s) | 393 |
| XapI | RAATTY | 1 cut(s) | 334 |
| XceI | RCATGY | 6 cut(s) | 20, 130, 154, 180, 230, 287 |
| XspI | CTAG | 2 cut(s) | 203, 246 |
| Zsp2I | ATGCAT | 3 cut(s) | 6, 156, 289 |