pycom17g16540

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001078
Physical Location & Seq
Forward (+)
14613478 .. 14614714
1237 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g16540.7

Sequence Viewer

Length: 822 bp
ATGACCTTTTTAGTTAATAAAAATAAAAGAGGGTTTGAATTTCTAAGCCCTAGGGTTTCCACCATTTACAAAATAAAATATAATAAAACAAATCAGCCACTAATCCTACATTTTGGCCGTGCCTTCAAGAATGATTCAAAGCCAAAAAGAAAACCTAGCCGCAAATCTCATTACTTTTTCCCTTGTGCCGCAGCCACCATAACCATATACTCCCATTATTCAACCCTAGCCACCATCATTCTACCATTTTTCTCTCCCAACAGCCGCACTTTCTCCCTCCACTTTCCAGCCGTCACACCCCATTCACTCTCCCAATCCGTGCATTTTTCCATTCACCCTAATTTCATTCAGCCACCATTCTTCAAATCCGTGCACTCCCATTCCCCTCTGATTTATCCATACACTTTCCACCACTATAACCTAATTCACAAACAGCCGCACCATTCCATCCTAGCCAATTTCCAGCCTTCCTCCATCACTGCCGCAGCCATTCCACCACTCTCCACCACCTTACACACCTTCCTACACCACCACACAACCTTAAACACATCCAAAAACCACCCACAACCAATCTGCCGTAGCAAGGAGAAAAGGGAAGAGGAGCTTCGACGCGCACCAATTCAAGTGGATTCGTTGGGCGTTCTAGGTGTAATCAATCCTTTGTGCTTTATGTTTAAATTCAATTGTATTTCTCTTTTTTCGAAACCATGTTTATGCTTGCAATATGATTTGATTACATTTGGTTGGAATTTCATGAATGGTGAATTCAATTCTGAGAGTGCACGCTTAGTTTTCATGCATGAATATGATGCTAGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

274

Amino Acids

31.58

Weight (kDa)

9.82

Isoelectric Point (pI)

54.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 612
AciI CCGC 5 cut(s) 160, 189, 265, 437, 483
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 4 cut(s) 38, 677, 748, 764
AfiI CCNNNNNNNGG 1 cut(s) 583
AgsI TTSAA 8 cut(s) 38, 127, 138, 222, 364, 623, 682, 769
AluBI AGCT 1 cut(s) 604
AluI AGCT 1 cut(s) 604
Alw21I GWGCWC 2 cut(s) 375, 784
Alw44I GTGCAC 2 cut(s) 371, 780
AoxI GGCC 1 cut(s) 115
ApaLI GTGCAC 2 cut(s) 371, 780
ApeKI GCWGC 2 cut(s) 191, 485
ApoI RAATTY 4 cut(s) 38, 677, 748, 764
AspA2I CCTAGG 1 cut(s) 50
AspLEI GCGC 1 cut(s) 614
AsuHPI GGTGA 2 cut(s) 326, 773
AsuII TTCGAA 1 cut(s) 701
AvrII CCTAGG 1 cut(s) 50
BaeGI GKGCMC 2 cut(s) 375, 784
Bbv12I GWGCWC 2 cut(s) 375, 784
BbvI GCAGC 2 cut(s) 203, 497
BccI CCATC 3 cut(s) 242, 455, 482
BceAI ACGGC 3 cut(s) 102, 275, 561
BfaI CTAG 6 cut(s) 51, 156, 227, 452, 644, 813
BisI GCNGC 7 cut(s) 160, 189, 192, 265, 437, 483, 486
BlnI CCTAGG 1 cut(s) 50
BlsI GCNGC 7 cut(s) 161, 190, 193, 266, 438, 484, 487
BmsI GCATC 1 cut(s) 799
Bpu14I TTCGAA 1 cut(s) 701
BsaJI CCNNGG 1 cut(s) 50
Bsc4I CCNNNNNNNGG 1 cut(s) 583
BseDI CCNNGG 1 cut(s) 50
BseGI GGATG 2 cut(s) 447, 548
BseLI CCNNNNNNNGG 1 cut(s) 583
BseMII CTCAG 1 cut(s) 765
BseRI GAGGAG 1 cut(s) 614
BseSI GKGCMC 2 cut(s) 375, 784
BseXI GCAGC 2 cut(s) 203, 497
Bsh1236I CGCG 1 cut(s) 612
BshFI GGCC 1 cut(s) 117
BsiHKAI GWGCWC 2 cut(s) 375, 784
BslI CCNNNNNNNGG 1 cut(s) 583
BsnI GGCC 1 cut(s) 117
Bsp119I TTCGAA 1 cut(s) 701
Bsp1286I GDGCHC 2 cut(s) 375, 784
BspACI CCGC 5 cut(s) 160, 189, 265, 437, 483
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 1 cut(s) 766
BspFNI CGCG 1 cut(s) 612
BspHI TCATGA 1 cut(s) 753
BspT104I TTCGAA 1 cut(s) 701
BssECI CCNNGG 1 cut(s) 50
BssT1I CCWWGG 1 cut(s) 50
Bst6I CTCTTC 1 cut(s) 591
BstBI TTCGAA 1 cut(s) 701
BstC8I GCNNGC 2 cut(s) 719, 784
BstDEI CTNAG 3 cut(s) 44, 774, 787
BstF5I GGATG 2 cut(s) 447, 548
BstFNI CGCG 1 cut(s) 612
BstHHI GCGC 1 cut(s) 614
BstSLI GKGCMC 2 cut(s) 375, 784
BstUI CGCG 1 cut(s) 612
BstV1I GCAGC 2 cut(s) 203, 497
BsuRI GGCC 1 cut(s) 117
BtsCI GGATG 2 cut(s) 447, 548
BtsI GCAGTG 1 cut(s) 477
BtsIMutI CAGTG 1 cut(s) 477
Cac8I GCNNGC 2 cut(s) 719, 784
CciI TCATGA 1 cut(s) 753
CfoI GCGC 1 cut(s) 614
CseI GACGC 1 cut(s) 618
CspCI CAANNNNNGTGG 2 cut(s) 606, 641
CviAII CATG 4 cut(s) 708, 754, 796, 800
DdeI CTNAG 3 cut(s) 44, 774, 787
DraI TTTAAA 1 cut(s) 676
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 1 cut(s) 591
EarI CTCTTC 1 cut(s) 591
Eco130I CCWWGG 1 cut(s) 50
EcoRI GAATTC 1 cut(s) 764
EcoT14I CCWWGG 1 cut(s) 50
EcoT22I ATGCAT 1 cut(s) 801
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 4 cut(s) 711, 757, 799, 803
FalI AAGNNNNNCTT 2 cut(s) 588, 620
FatI CATG 4 cut(s) 707, 753, 795, 799
Fnu4HI GCNGC 7 cut(s) 160, 189, 192, 265, 437, 483, 486
FokI GGATG 2 cut(s) 434, 535
Fsp4HI GCNGC 7 cut(s) 160, 189, 192, 265, 437, 483, 486
FspBI CTAG 6 cut(s) 51, 156, 227, 452, 644, 813
GlaI GCGC 1 cut(s) 613
GluI GCNGC 7 cut(s) 160, 189, 192, 265, 437, 483, 486
HaeIII GGCC 1 cut(s) 117
HgaI GACGC 1 cut(s) 618
HhaI GCGC 1 cut(s) 614
Hin1II CATG 4 cut(s) 711, 757, 799, 803
Hin6I GCGC 1 cut(s) 612
HinP1I GCGC 1 cut(s) 612
HinfI GANTC 2 cut(s) 134, 629
HphI GGTGA 2 cut(s) 326, 773
Hpy166II GTNNAC 2 cut(s) 373, 782
Hpy188I TCNGA 2 cut(s) 390, 775
Hpy188III TCNNGA 2 cut(s) 127, 754
Hpy8I GTNNAC 2 cut(s) 373, 782
Hpy99I CGWCG 1 cut(s) 612
HpyAV CCTTC 3 cut(s) 133, 477, 529
HpyCH4V TGCA 5 cut(s) 322, 373, 721, 782, 799
HpyF3I CTNAG 3 cut(s) 44, 774, 787
Hsp92II CATG 4 cut(s) 711, 757, 799, 803
HspAI GCGC 1 cut(s) 612
LmnI GCTCC 1 cut(s) 601
LpnPI CCDG 2 cut(s) 300, 476
Lsp1109I GCAGC 2 cut(s) 203, 497
LweI GCATC 1 cut(s) 799
MaeI CTAG 6 cut(s) 51, 156, 227, 452, 644, 813
MaeIII GTNAC 1 cut(s) 292
MboII GAAGA 2 cut(s) 352, 608
MfeI CAATTG 1 cut(s) 682
MhlI GDGCHC 2 cut(s) 375, 784
MmeI TCCRAC 1 cut(s) 725
MnlI CCTC 5 cut(s) 23, 287, 396, 481, 592
Mph1103I ATGCAT 1 cut(s) 801
MseI TTAA 3 cut(s) 15, 542, 675
MslI CAYNNNNRTG 2 cut(s) 712, 804
MunI CAATTG 1 cut(s) 682
MvnI CGCG 1 cut(s) 612
NlaIII CATG 4 cut(s) 711, 757, 799, 803
NmuCI GTSAC 1 cut(s) 292
NsiI ATGCAT 1 cut(s) 801
NspV TTCGAA 1 cut(s) 701
PagI TCATGA 1 cut(s) 753
PfeI GAWTC 2 cut(s) 134, 629
PkrI GCNGC 7 cut(s) 161, 190, 193, 266, 438, 484, 487
RseI CAYNNNNRTG 2 cut(s) 712, 804
SaqAI TTAA 3 cut(s) 15, 542, 675
SatI GCNGC 7 cut(s) 160, 189, 192, 265, 437, 483, 486
SduI GDGCHC 2 cut(s) 375, 784
SetI ASST 8 cut(s) 8, 157, 423, 512, 521, 542, 606, 649
SfaNI GCATC 1 cut(s) 799
SfuI TTCGAA 1 cut(s) 701
SmiMI CAYNNNNRTG 2 cut(s) 712, 804
SsiI CCGC 5 cut(s) 160, 189, 265, 437, 483
SspMI CTAG 6 cut(s) 51, 156, 227, 452, 644, 813
StyI CCWWGG 1 cut(s) 50
TaqI TCGA 2 cut(s) 607, 701
TauI GCSGC 5 cut(s) 162, 191, 267, 439, 485
TfiI GAWTC 2 cut(s) 134, 629
Tru1I TTAA 3 cut(s) 15, 542, 675
Tru9I TTAA 3 cut(s) 15, 542, 675
TscAI CASTG 1 cut(s) 484
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 2 cut(s) 191, 485
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 5 cut(s) 334, 742, 770, 784, 816
TspGWI ACGGA 2 cut(s) 307, 358
TspRI CASTG 1 cut(s) 484
VneI GTGCAC 2 cut(s) 371, 780
XapI RAATTY 4 cut(s) 38, 677, 748, 764
XmaJI CCTAGG 1 cut(s) 50
XspI CTAG 6 cut(s) 51, 156, 227, 452, 644, 813
Zsp2I ATGCAT 1 cut(s) 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.