pycom2328g00050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002328
OG0001078
Physical Location & Seq
Forward (+)
76665 .. 77622
958 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2328g00050.2

Sequence Viewer

Length: 726 bp
ATGCTTTCCCATTTCATTATTCATTCACTTTGCAGCCACCATTCCCCCTTTAATCCACATCCACACCTTCACCCACATGCACCATTCAAACATACACCCAAACACCACCAGAAATCATCATTGCCGTGGGTACCTATCCCATTTCAGATTGTCATTCCACTTCATTGCACATGCATCTTCATAATTCAACACATGCATTCATTATTTATTTACTTTCATTCCAGCCATACCTTGCATTTTCAGATTGTTCCTCCATCATTGCACCACAATGCAGCCACATGCACTTGTTCCTCTAACATTTCAGCCACTTGCACTCCCATTCTCTCCCAAAACCGTGCACATGCATTCTCCTTGCATTTCCAGCCATTCCACATGCTATTTCAGCCACATGCACTCATAATCATTCAACCAACTTTGCACATAAGCATCCATTGCATTCATTCTCATCCATACATTCATTCACAACAGAAATTGGTCCCTTGGGGCCGTGCCCTTCACAAAGTCCATCCATTCCATTCATACACTCATCCATTGCAGAAATGTAGACCATCAAACCATCCTTGTGCCGTGCTCTCCCTTACAATCCAAACACCATCCTTCCATTTCCACCACTCCCCAAACCATTCACACCATCAAACTATCCCTAGACCTTGTGCTACAACGAAGAGGAAGAGAAGAGTGCCTAAACGTTCATACAATTCAAGTTTGAGTTGTTGGAATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

242

Amino Acids

27.86

Weight (kDa)

9.94

Isoelectric Point (pI)

54.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 130
AccB1I GGYRCC 1 cut(s) 130
AccI GTMKAC 1 cut(s) 544
AclI AACGTT 1 cut(s) 688
AfaI GTAC 1 cut(s) 132
AgsI TTSAA 4 cut(s) 88, 188, 407, 702
Alw21I GWGCWC 2 cut(s) 340, 573
Alw44I GTGCAC 1 cut(s) 336
AoxI GGCC 1 cut(s) 484
ApaLI GTGCAC 1 cut(s) 336
ApeKI GCWGC 2 cut(s) 33, 272
Asp718I GGTACC 1 cut(s) 130
AspS9I GGNCC 2 cut(s) 475, 484
AsuHPI GGTGA 1 cut(s) 62
AvaII GGWCC 1 cut(s) 475
BaeGI GKGCMC 2 cut(s) 340, 493
BanI GGYRCC 1 cut(s) 130
Bbv12I GWGCWC 2 cut(s) 340, 573
BbvI GCAGC 2 cut(s) 45, 284
BccI CCATC 6 cut(s) 262, 513, 556, 564, 601, 639
BceAI ACGGC 3 cut(s) 109, 471, 551
BfaI CTAG 1 cut(s) 645
BisI GCNGC 2 cut(s) 34, 273
BlsI GCNGC 2 cut(s) 35, 274
Bme18I GGWCC 1 cut(s) 475
BmgT120I GGNCC 2 cut(s) 475, 484
BmiI GGNNCC 3 cut(s) 132, 477, 485
BmsI GCATC 2 cut(s) 183, 435
BsaJI CCNNGG 2 cut(s) 125, 479
BsaXI ACNNNNNCTCC 2 cut(s) 298, 328
Bse3DI GCAATG 5 cut(s) 119, 163, 257, 430, 530
BseDI CCNNGG 2 cut(s) 125, 479
BseGI GGATG 7 cut(s) 58, 426, 445, 505, 526, 556, 593
BseMI GCAATG 5 cut(s) 119, 163, 257, 430, 530
BseSI GKGCMC 2 cut(s) 340, 493
BseXI GCAGC 2 cut(s) 45, 284
BshFI GGCC 1 cut(s) 486
BshNI GGYRCC 1 cut(s) 130
BsiHKAI GWGCWC 2 cut(s) 340, 573
BslFI GGGAC 1 cut(s) 461
BsmFI GGGAC 1 cut(s) 461
BsmI GAATGC 3 cut(s) 196, 344, 435
BsnI GGCC 1 cut(s) 486
Bsp1286I GDGCHC 3 cut(s) 340, 493, 573
BspANI GGCC 1 cut(s) 486
BspLI GGNNCC 3 cut(s) 132, 477, 485
BspT107I GGYRCC 1 cut(s) 130
BsrDI GCAATG 5 cut(s) 119, 163, 257, 430, 530
BssECI CCNNGG 2 cut(s) 125, 479
BssT1I CCWWGG 1 cut(s) 479
Bst4CI ACNGT 1 cut(s) 335
Bst6I CTCTTC 3 cut(s) 659, 665, 670
BstAPI GCANNNNNTGC 1 cut(s) 432
BstDSI CCRYGG 1 cut(s) 125
BstF5I GGATG 7 cut(s) 58, 426, 445, 505, 526, 556, 593
BstMWI GCNNNNNNNGC 3 cut(s) 361, 382, 432
BstNSI RCATGY 7 cut(s) 80, 174, 196, 282, 344, 376, 392
BstSLI GKGCMC 2 cut(s) 340, 493
BstV1I GCAGC 2 cut(s) 45, 284
BsuRI GGCC 1 cut(s) 486
BtgI CCRYGG 1 cut(s) 125
BtsCI GGATG 7 cut(s) 58, 426, 445, 505, 526, 556, 593
Cfr13I GGNCC 2 cut(s) 475, 484
Csp6I GTAC 1 cut(s) 131
CviAII CATG 7 cut(s) 77, 171, 193, 279, 341, 373, 389
CviJI RGCY 7 cut(s) 36, 225, 275, 305, 364, 385, 486
CviKI_1 RGCY 7 cut(s) 36, 225, 275, 305, 364, 385, 486
CviQI GTAC 1 cut(s) 131
Eam1104I CTCTTC 3 cut(s) 659, 665, 670
EarI CTCTTC 3 cut(s) 659, 665, 670
Eco130I CCWWGG 1 cut(s) 479
Eco47I GGWCC 1 cut(s) 475
EcoT14I CCWWGG 1 cut(s) 479
EcoT22I ATGCAT 3 cut(s) 176, 198, 346
ErhI CCWWGG 1 cut(s) 479
FaeI CATG 7 cut(s) 80, 174, 196, 282, 344, 376, 392
FaqI GGGAC 1 cut(s) 461
FatI CATG 7 cut(s) 76, 170, 192, 278, 340, 372, 388
FblI GTMKAC 1 cut(s) 544
Fnu4HI GCNGC 2 cut(s) 34, 273
FokI GGATG 7 cut(s) 45, 413, 432, 492, 513, 543, 580
Fsp4HI GCNGC 2 cut(s) 34, 273
FspBI CTAG 1 cut(s) 645
GluI GCNGC 2 cut(s) 34, 273
HaeIII GGCC 1 cut(s) 486
Hin1II CATG 7 cut(s) 80, 174, 196, 282, 344, 376, 392
HphI GGTGA 1 cut(s) 62
Hpy166II GTNNAC 2 cut(s) 338, 545
Hpy188I TCNGA 2 cut(s) 147, 243
Hpy8I GTNNAC 2 cut(s) 338, 545
HpyAV CCTTC 3 cut(s) 77, 503, 607
HpyCH4III ACNGT 1 cut(s) 335
HpyCH4IV ACGT 1 cut(s) 688
HpyF10VI GCNNNNNNNGC 3 cut(s) 361, 382, 432
HpySE526I ACGT 1 cut(s) 688
Hsp92II CATG 7 cut(s) 80, 174, 196, 282, 344, 376, 392
KpnI GGTACC 1 cut(s) 134
LpnPI CCDG 3 cut(s) 122, 235, 374
Lsp1109I GCAGC 2 cut(s) 45, 284
LweI GCATC 2 cut(s) 183, 435
MaeI CTAG 1 cut(s) 645
MaeII ACGT 1 cut(s) 688
MboII GAAGA 4 cut(s) 169, 676, 682, 687
MhlI GDGCHC 3 cut(s) 340, 493, 573
MluCI AATT 3 cut(s) 183, 470, 697
MmeI TCCRAC 1 cut(s) 695
MnlI CCTC 3 cut(s) 261, 301, 660
Mph1103I ATGCAT 3 cut(s) 176, 198, 346
MseI TTAA 1 cut(s) 51
MslI CAYNNNNRTG 4 cut(s) 75, 124, 267, 561
Mva1269I GAATGC 3 cut(s) 196, 344, 435
MwoI GCNNNNNNNGC 3 cut(s) 361, 382, 432
NlaIII CATG 7 cut(s) 80, 174, 196, 282, 344, 376, 392
NlaIV GGNNCC 3 cut(s) 132, 477, 485
NsiI ATGCAT 3 cut(s) 176, 198, 346
NspI RCATGY 7 cut(s) 80, 174, 196, 282, 344, 376, 392
PctI GAATGC 3 cut(s) 196, 344, 435
PkrI GCNGC 2 cut(s) 35, 274
Psp1406I AACGTT 1 cut(s) 688
PspN4I GGNNCC 3 cut(s) 132, 477, 485
PspPI GGNCC 2 cut(s) 475, 484
RsaI GTAC 1 cut(s) 132
RsaNI GTAC 1 cut(s) 131
RseI CAYNNNNRTG 4 cut(s) 75, 124, 267, 561
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 2 cut(s) 34, 273
Sau96I GGNCC 2 cut(s) 475, 484
SduI GDGCHC 3 cut(s) 340, 493, 573
SetI ASST 5 cut(s) 69, 136, 233, 652, 691
SfaNI GCATC 2 cut(s) 183, 435
SinI GGWCC 1 cut(s) 475
SmiMI CAYNNNNRTG 4 cut(s) 75, 124, 267, 561
Sse9I AATT 3 cut(s) 183, 470, 697
SspMI CTAG 1 cut(s) 645
StyI CCWWGG 1 cut(s) 479
TaaI ACNGT 1 cut(s) 335
TaiI ACGT 1 cut(s) 691
TasI AATT 3 cut(s) 183, 470, 697
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TseI GCWGC 2 cut(s) 33, 272
VneI GTGCAC 1 cut(s) 336
VpaK11BI GGWCC 1 cut(s) 475
XceI RCATGY 7 cut(s) 80, 174, 196, 282, 344, 376, 392
XmiI GTMKAC 1 cut(s) 544
XspI CTAG 1 cut(s) 645
Zsp2I ATGCAT 3 cut(s) 176, 198, 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.