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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclI | AACGTT | 1 cut(s) | 307 |
| AclWI | GGATC | 1 cut(s) | 158 |
| AgsI | TTSAA | 2 cut(s) | 147, 321 |
| AluBI | AGCT | 1 cut(s) | 26 |
| AluI | AGCT | 1 cut(s) | 26 |
| AlwI | GGATC | 1 cut(s) | 158 |
| AspS9I | GGNCC | 1 cut(s) | 299 |
| AsuHPI | GGTGA | 1 cut(s) | 87 |
| AvaII | GGWCC | 1 cut(s) | 299 |
| BccI | CCATC | 2 cut(s) | 43, 221 |
| BfaI | CTAG | 1 cut(s) | 23 |
| Bme18I | GGWCC | 1 cut(s) | 299 |
| BmgT120I | GGNCC | 1 cut(s) | 299 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 91, 121 |
| BseRI | GAGGAG | 1 cut(s) | 210 |
| BsmI | GAATGC | 2 cut(s) | 4, 134 |
| Bsp143I | GATC | 1 cut(s) | 150 |
| BspPI | GGATC | 1 cut(s) | 158 |
| BssMI | GATC | 1 cut(s) | 150 |
| Bst6I | CTCTTC | 2 cut(s) | 284, 289 |
| BstDEI | CTNAG | 1 cut(s) | 263 |
| BstKTI | GATC | 1 cut(s) | 153 |
| BstMBI | GATC | 1 cut(s) | 150 |
| BstNSI | RCATGY | 1 cut(s) | 56 |
| Cfr13I | GGNCC | 1 cut(s) | 299 |
| CviAII | CATG | 1 cut(s) | 53 |
| CviJI | RGCY | 1 cut(s) | 26 |
| CviKI_1 | RGCY | 1 cut(s) | 26 |
| DdeI | CTNAG | 1 cut(s) | 263 |
| DpnI | GATC | 1 cut(s) | 152 |
| DpnII | GATC | 1 cut(s) | 150 |
| Eam1104I | CTCTTC | 2 cut(s) | 284, 289 |
| EarI | CTCTTC | 2 cut(s) | 284, 289 |
| Eco47I | GGWCC | 1 cut(s) | 299 |
| EcoO109I | RGGNCCY | 1 cut(s) | 299 |
| EcoT22I | ATGCAT | 1 cut(s) | 6 |
| FaeI | CATG | 1 cut(s) | 56 |
| FaiI | YATR | 7 cut(s) | 48, 54, 68, 103, 122, 273, 313 |
| FatI | CATG | 1 cut(s) | 52 |
| FspBI | CTAG | 1 cut(s) | 23 |
| Hin1II | CATG | 1 cut(s) | 56 |
| HphI | GGTGA | 1 cut(s) | 87 |
| HpyCH4IV | ACGT | 1 cut(s) | 307 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 56, 134 |
| HpyF3I | CTNAG | 1 cut(s) | 263 |
| HpySE526I | ACGT | 1 cut(s) | 307 |
| Hsp92II | CATG | 1 cut(s) | 56 |
| Kzo9I | GATC | 1 cut(s) | 150 |
| MaeI | CTAG | 1 cut(s) | 23 |
| MaeII | ACGT | 1 cut(s) | 307 |
| MalI | GATC | 1 cut(s) | 152 |
| MboI | GATC | 1 cut(s) | 150 |
| MboII | GAAGA | 2 cut(s) | 301, 306 |
| MluCI | AATT | 1 cut(s) | 316 |
| MmeI | TCCRAC | 1 cut(s) | 314 |
| MnlI | CCTC | 4 cut(s) | 187, 231, 279, 290 |
| Mph1103I | ATGCAT | 1 cut(s) | 6 |
| MseI | TTAA | 1 cut(s) | 87 |
| MslI | CAYNNNNRTG | 1 cut(s) | 51 |
| Mva1269I | GAATGC | 2 cut(s) | 4, 134 |
| NdeII | GATC | 1 cut(s) | 150 |
| NlaIII | CATG | 1 cut(s) | 56 |
| NsiI | ATGCAT | 1 cut(s) | 6 |
| NspI | RCATGY | 1 cut(s) | 56 |
| PctI | GAATGC | 2 cut(s) | 4, 134 |
| PpuMI | RGGWCCY | 1 cut(s) | 299 |
| Psp1406I | AACGTT | 1 cut(s) | 307 |
| Psp5II | RGGWCCY | 1 cut(s) | 299 |
| PspPI | GGNCC | 1 cut(s) | 299 |
| PspPPI | RGGWCCY | 1 cut(s) | 299 |
| RseI | CAYNNNNRTG | 1 cut(s) | 51 |
| SaqAI | TTAA | 1 cut(s) | 87 |
| Sau3AI | GATC | 1 cut(s) | 150 |
| Sau96I | GGNCC | 1 cut(s) | 299 |
| SetI | ASST | 5 cut(s) | 24, 28, 271, 304, 310 |
| SgeI | CNNG | 6 cut(s) | 35, 65, 143, 159, 297, 333 |
| SinI | GGWCC | 1 cut(s) | 299 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 51 |
| Sse9I | AATT | 1 cut(s) | 316 |
| SspMI | CTAG | 1 cut(s) | 23 |
| TaiI | ACGT | 1 cut(s) | 310 |
| TasI | AATT | 1 cut(s) | 316 |
| Tru1I | TTAA | 1 cut(s) | 87 |
| Tru9I | TTAA | 1 cut(s) | 87 |
| TspDTI | ATGAA | 4 cut(s) | 35, 127, 131, 300 |
| VpaK11BI | GGWCC | 1 cut(s) | 299 |
| XceI | RCATGY | 1 cut(s) | 56 |
| XspI | CTAG | 1 cut(s) | 23 |
| Zsp2I | ATGCAT | 1 cut(s) | 6 |