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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AluBI | AGCT | 3 cut(s) | 26, 161, 180 |
| AluI | AGCT | 3 cut(s) | 26, 161, 180 |
| ApeKI | GCWGC | 2 cut(s) | 137, 223 |
| AsuHPI | GGTGA | 2 cut(s) | 10, 192 |
| BbvI | GCAGC | 2 cut(s) | 149, 235 |
| BccI | CCATC | 1 cut(s) | 77 |
| BfaI | CTAG | 2 cut(s) | 23, 177 |
| BisI | GCNGC | 2 cut(s) | 138, 224 |
| BlsI | GCNGC | 2 cut(s) | 139, 225 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 68, 98 |
| Bse3DI | GCAATG | 2 cut(s) | 103, 218 |
| BseGI | GGATG | 1 cut(s) | 214 |
| BseMI | GCAATG | 2 cut(s) | 103, 218 |
| BseXI | GCAGC | 2 cut(s) | 149, 235 |
| BsmI | GAATGC | 3 cut(s) | 4, 114, 233 |
| BsrDI | GCAATG | 2 cut(s) | 103, 218 |
| BstF5I | GGATG | 1 cut(s) | 214 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 65, 158 |
| BstNSI | RCATGY | 5 cut(s) | 40, 82, 114, 152, 233 |
| BstV1I | GCAGC | 2 cut(s) | 149, 235 |
| BtsCI | GGATG | 1 cut(s) | 214 |
| CviAII | CATG | 5 cut(s) | 37, 79, 111, 149, 230 |
| CviJI | RGCY | 5 cut(s) | 26, 140, 161, 180, 226 |
| CviKI_1 | RGCY | 5 cut(s) | 26, 140, 161, 180, 226 |
| EcoT22I | ATGCAT | 4 cut(s) | 6, 116, 154, 235 |
| FaeI | CATG | 5 cut(s) | 40, 82, 114, 152, 233 |
| FaiI | YATR | 8 cut(s) | 38, 80, 112, 120, 150, 186, 206, 231 |
| FatI | CATG | 5 cut(s) | 36, 78, 110, 148, 229 |
| Fnu4HI | GCNGC | 2 cut(s) | 138, 224 |
| FokI | GGATG | 1 cut(s) | 201 |
| Fsp4HI | GCNGC | 2 cut(s) | 138, 224 |
| FspBI | CTAG | 2 cut(s) | 23, 177 |
| GluI | GCNGC | 2 cut(s) | 138, 224 |
| Hin1II | CATG | 5 cut(s) | 40, 82, 114, 152, 233 |
| HphI | GGTGA | 2 cut(s) | 10, 192 |
| HpyCH4V | TGCA | 9 cut(s) | 4, 40, 82, 108, 114, 137, 152, 223, 233 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 65, 158 |
| Hsp92II | CATG | 5 cut(s) | 40, 82, 114, 152, 233 |
| LpnPI | CCDG | 1 cut(s) | 171 |
| Lsp1109I | GCAGC | 2 cut(s) | 149, 235 |
| MaeI | CTAG | 2 cut(s) | 23, 177 |
| MluCI | AATT | 1 cut(s) | 30 |
| MnlI | CCTC | 2 cut(s) | 183, 202 |
| Mph1103I | ATGCAT | 4 cut(s) | 6, 116, 154, 235 |
| MseI | TTAA | 2 cut(s) | 29, 244 |
| MslI | CAYNNNNRTG | 1 cut(s) | 77 |
| Mva1269I | GAATGC | 3 cut(s) | 4, 114, 233 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 65, 158 |
| NlaIII | CATG | 5 cut(s) | 40, 82, 114, 152, 233 |
| NsiI | ATGCAT | 4 cut(s) | 6, 116, 154, 235 |
| NspI | RCATGY | 5 cut(s) | 40, 82, 114, 152, 233 |
| PctI | GAATGC | 3 cut(s) | 4, 114, 233 |
| PkrI | GCNGC | 2 cut(s) | 139, 225 |
| RseI | CAYNNNNRTG | 1 cut(s) | 77 |
| SaqAI | TTAA | 2 cut(s) | 29, 244 |
| SatI | GCNGC | 2 cut(s) | 138, 224 |
| SetI | ASST | 6 cut(s) | 28, 135, 163, 175, 182, 205 |
| SgeI | CNNG | 9 cut(s) | 35, 49, 91, 123, 146, 161, 170, 189, 242 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 77 |
| Sse9I | AATT | 1 cut(s) | 30 |
| SspMI | CTAG | 2 cut(s) | 23, 177 |
| TasI | AATT | 1 cut(s) | 30 |
| Tru1I | TTAA | 2 cut(s) | 29, 244 |
| Tru9I | TTAA | 2 cut(s) | 29, 244 |
| TseI | GCWGC | 2 cut(s) | 137, 223 |
| TspDTI | ATGAA | 2 cut(s) | 39, 107 |
| XceI | RCATGY | 5 cut(s) | 40, 82, 114, 152, 233 |
| XspI | CTAG | 2 cut(s) | 23, 177 |
| Zsp2I | ATGCAT | 4 cut(s) | 6, 116, 154, 235 |