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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AsuHPI | GGTGA | 2 cut(s) | 24, 170 |
| BmsI | GCATC | 1 cut(s) | 81 |
| Bse3DI | GCAATG | 3 cut(s) | 67, 107, 147 |
| BseMI | GCAATG | 3 cut(s) | 67, 107, 147 |
| BsmI | GAATGC | 1 cut(s) | 22 |
| BsrDI | GCAATG | 3 cut(s) | 67, 107, 147 |
| BstNSI | RCATGY | 4 cut(s) | 52, 92, 132, 172 |
| CviAII | CATG | 4 cut(s) | 49, 89, 129, 169 |
| CviJI | RGCY | 4 cut(s) | 45, 85, 125, 165 |
| CviKI_1 | RGCY | 4 cut(s) | 45, 85, 125, 165 |
| EcoT22I | ATGCAT | 1 cut(s) | 54 |
| FaeI | CATG | 4 cut(s) | 52, 92, 132, 172 |
| FaiI | YATR | 5 cut(s) | 50, 90, 130, 170, 196 |
| FatI | CATG | 4 cut(s) | 48, 88, 128, 168 |
| Hin1II | CATG | 4 cut(s) | 52, 92, 132, 172 |
| HphI | GGTGA | 2 cut(s) | 24, 170 |
| Hpy188I | TCNGA | 1 cut(s) | 17 |
| HpyCH4V | TGCA | 8 cut(s) | 22, 52, 72, 92, 112, 132, 152, 172 |
| Hsp92II | CATG | 4 cut(s) | 52, 92, 132, 172 |
| LweI | GCATC | 1 cut(s) | 81 |
| Mph1103I | ATGCAT | 1 cut(s) | 54 |
| Mva1269I | GAATGC | 1 cut(s) | 22 |
| NlaIII | CATG | 4 cut(s) | 52, 92, 132, 172 |
| NsiI | ATGCAT | 1 cut(s) | 54 |
| NspI | RCATGY | 4 cut(s) | 52, 92, 132, 172 |
| PctI | GAATGC | 1 cut(s) | 22 |
| SetI | ASST | 1 cut(s) | 195 |
| SfaNI | GCATC | 1 cut(s) | 81 |
| SgeI | CNNG | 4 cut(s) | 61, 101, 141, 181 |
| TspDTI | ATGAA | 3 cut(s) | 53, 93, 133 |
| XceI | RCATGY | 4 cut(s) | 52, 92, 132, 172 |
| Zsp2I | ATGCAT | 1 cut(s) | 54 |