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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 2 cut(s) | 92, 128 |
| AluBI | AGCT | 1 cut(s) | 236 |
| AluI | AGCT | 1 cut(s) | 236 |
| ApeKI | GCWGC | 3 cut(s) | 111, 185, 347 |
| AsuHPI | GGTGA | 3 cut(s) | 220, 399, 545 |
| BbvI | GCAGC | 3 cut(s) | 123, 197, 359 |
| BccI | CCATC | 2 cut(s) | 73, 287 |
| BfaI | CTAG | 1 cut(s) | 233 |
| BisI | GCNGC | 3 cut(s) | 112, 186, 348 |
| BlsI | GCNGC | 3 cut(s) | 113, 187, 349 |
| BmiI | GGNNCC | 2 cut(s) | 23, 70 |
| BmsI | GCATC | 1 cut(s) | 456 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 278, 308, 365, 395 |
| Bse3DI | GCAATG | 6 cut(s) | 106, 313, 342, 442, 482, 522 |
| BseGI | GGATG | 3 cut(s) | 16, 84, 338 |
| BseMI | GCAATG | 6 cut(s) | 106, 313, 342, 442, 482, 522 |
| BseXI | GCAGC | 3 cut(s) | 123, 197, 359 |
| BsmI | GAATGC | 4 cut(s) | 214, 324, 357, 397 |
| BspLI | GGNNCC | 2 cut(s) | 23, 70 |
| BsrDI | GCAATG | 6 cut(s) | 106, 313, 342, 442, 482, 522 |
| BstF5I | GGATG | 3 cut(s) | 16, 84, 338 |
| BstV1I | GCAGC | 3 cut(s) | 123, 197, 359 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 79 |
| BtsCI | GGATG | 3 cut(s) | 16, 84, 338 |
| Eco32I | GATATC | 1 cut(s) | 56 |
| EcoRV | GATATC | 1 cut(s) | 56 |
| EcoT22I | ATGCAT | 4 cut(s) | 216, 326, 359, 429 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 31 |
| FauNDI | CATATG | 1 cut(s) | 60 |
| Fnu4HI | GCNGC | 3 cut(s) | 112, 186, 348 |
| FokI | GGATG | 3 cut(s) | 23, 91, 325 |
| Fsp4HI | GCNGC | 3 cut(s) | 112, 186, 348 |
| FspBI | CTAG | 1 cut(s) | 233 |
| GluI | GCNGC | 3 cut(s) | 112, 186, 348 |
| HinfI | GANTC | 1 cut(s) | 96 |
| HphI | GGTGA | 3 cut(s) | 220, 399, 545 |
| Hpy188I | TCNGA | 1 cut(s) | 392 |
| HpyAV | CCTTC | 1 cut(s) | 86 |
| LmnI | GCTCC | 3 cut(s) | 21, 31, 68 |
| Lsp1109I | GCAGC | 3 cut(s) | 123, 197, 359 |
| LweI | GCATC | 1 cut(s) | 456 |
| MaeI | CTAG | 1 cut(s) | 233 |
| MluCI | AATT | 3 cut(s) | 36, 87, 240 |
| MnlI | CCTC | 1 cut(s) | 69 |
| Mph1103I | ATGCAT | 4 cut(s) | 216, 326, 359, 429 |
| MseI | TTAA | 3 cut(s) | 203, 239, 368 |
| MslI | CAYNNNNRTG | 1 cut(s) | 287 |
| Mva1269I | GAATGC | 4 cut(s) | 214, 324, 357, 397 |
| NdeI | CATATG | 1 cut(s) | 60 |
| NlaIV | GGNNCC | 2 cut(s) | 23, 70 |
| NsiI | ATGCAT | 4 cut(s) | 216, 326, 359, 429 |
| PctI | GAATGC | 4 cut(s) | 214, 324, 357, 397 |
| PfeI | GAWTC | 1 cut(s) | 96 |
| PkrI | GCNGC | 3 cut(s) | 113, 187, 349 |
| PspN4I | GGNNCC | 2 cut(s) | 23, 70 |
| RseI | CAYNNNNRTG | 1 cut(s) | 287 |
| SaqAI | TTAA | 3 cut(s) | 203, 239, 368 |
| SatI | GCNGC | 3 cut(s) | 112, 186, 348 |
| SetI | ASST | 3 cut(s) | 148, 238, 570 |
| SfaNI | GCATC | 1 cut(s) | 456 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 287 |
| Sse9I | AATT | 3 cut(s) | 36, 87, 240 |
| SspMI | CTAG | 1 cut(s) | 233 |
| TasI | AATT | 3 cut(s) | 36, 87, 240 |
| TfiI | GAWTC | 1 cut(s) | 96 |
| Tru1I | TTAA | 3 cut(s) | 203, 239, 368 |
| Tru9I | TTAA | 3 cut(s) | 203, 239, 368 |
| TseI | GCWGC | 3 cut(s) | 111, 185, 347 |
| TspDTI | ATGAA | 9 cut(s) | 156, 163, 207, 249, 317, 327, 428, 468, 508 |
| XspI | CTAG | 1 cut(s) | 233 |
| Zsp2I | ATGCAT | 4 cut(s) | 216, 326, 359, 429 |