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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclI | AACGTT | 1 cut(s) | 828 |
| AcvI | CACGTG | 1 cut(s) | 478 |
| AgsI | TTSAA | 3 cut(s) | 116, 219, 842 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 537, 569 |
| AluBI | AGCT | 1 cut(s) | 446 |
| AluI | AGCT | 1 cut(s) | 446 |
| ApeKI | GCWGC | 2 cut(s) | 443, 446 |
| AsuHPI | GGTGA | 5 cut(s) | 21, 91, 194, 449, 772 |
| BbrPI | CACGTG | 1 cut(s) | 478 |
| BbvI | GCAGC | 2 cut(s) | 433, 455 |
| BccI | CCATC | 2 cut(s) | 34, 165 |
| BceAI | ACGGC | 2 cut(s) | 34, 679 |
| BfaI | CTAG | 2 cut(s) | 39, 92 |
| BisI | GCNGC | 2 cut(s) | 444, 447 |
| BlsI | GCNGC | 2 cut(s) | 445, 448 |
| BmsI | GCATC | 4 cut(s) | 63, 458, 638, 708 |
| BsaAI | YACGTR | 1 cut(s) | 478 |
| BsaJI | CCNNGG | 1 cut(s) | 508 |
| BseDI | CCNNGG | 1 cut(s) | 508 |
| BseGI | GGATG | 5 cut(s) | 54, 449, 503, 629, 765 |
| BseRI | GAGGAG | 1 cut(s) | 752 |
| BseXI | GCAGC | 2 cut(s) | 433, 455 |
| BsmI | GAATGC | 3 cut(s) | 187, 320, 343 |
| BssECI | CCNNGG | 1 cut(s) | 508 |
| BssT1I | CCWWGG | 1 cut(s) | 508 |
| Bst4CI | ACNGT | 1 cut(s) | 549 |
| Bst6I | CTCTTC | 3 cut(s) | 733, 805, 810 |
| BstBAI | YACGTR | 1 cut(s) | 478 |
| BstC8I | GCNNGC | 3 cut(s) | 341, 515, 578 |
| BstDEI | CTNAG | 1 cut(s) | 784 |
| BstF5I | GGATG | 5 cut(s) | 54, 449, 503, 629, 765 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 336, 359, 421 |
| BstV1I | GCAGC | 2 cut(s) | 433, 455 |
| BtsCI | GGATG | 5 cut(s) | 54, 449, 503, 629, 765 |
| BtsI | GCAGTG | 1 cut(s) | 430 |
| BtsIMutI | CAGTG | 1 cut(s) | 430 |
| Cac8I | GCNNGC | 3 cut(s) | 341, 515, 578 |
| CviJI | RGCY | 7 cut(s) | 42, 156, 259, 424, 446, 569, 593 |
| CviKI_1 | RGCY | 7 cut(s) | 42, 156, 259, 424, 446, 569, 593 |
| DdeI | CTNAG | 1 cut(s) | 784 |
| Eam1104I | CTCTTC | 3 cut(s) | 733, 805, 810 |
| EarI | CTCTTC | 3 cut(s) | 733, 805, 810 |
| Eco130I | CCWWGG | 1 cut(s) | 508 |
| Eco72I | CACGTG | 1 cut(s) | 478 |
| EcoT14I | CCWWGG | 1 cut(s) | 508 |
| EcoT22I | ATGCAT | 5 cut(s) | 189, 345, 355, 417, 578 |
| ErhI | CCWWGG | 1 cut(s) | 508 |
| Fnu4HI | GCNGC | 2 cut(s) | 444, 447 |
| FokI | GGATG | 5 cut(s) | 41, 436, 490, 616, 752 |
| Fsp4HI | GCNGC | 2 cut(s) | 444, 447 |
| FspBI | CTAG | 2 cut(s) | 39, 92 |
| GluI | GCNGC | 2 cut(s) | 444, 447 |
| HphI | GGTGA | 5 cut(s) | 21, 91, 194, 449, 772 |
| Hpy188I | TCNGA | 1 cut(s) | 74 |
| HpyAV | CCTTC | 5 cut(s) | 203, 416, 435, 613, 716 |
| HpyCH4III | ACNGT | 1 cut(s) | 549 |
| HpyCH4IV | ACGT | 2 cut(s) | 477, 828 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 336, 359, 421 |
| HpyF3I | CTNAG | 1 cut(s) | 784 |
| HpySE526I | ACGT | 2 cut(s) | 477, 828 |
| LpnPI | CCDG | 7 cut(s) | 166, 269, 330, 349, 413, 434, 579 |
| Lsp1109I | GCAGC | 2 cut(s) | 433, 455 |
| LweI | GCATC | 4 cut(s) | 63, 458, 638, 708 |
| MaeI | CTAG | 2 cut(s) | 39, 92 |
| MaeII | ACGT | 2 cut(s) | 477, 828 |
| MaeIII | GTNAC | 1 cut(s) | 97 |
| MboII | GAAGA | 5 cut(s) | 134, 237, 720, 822, 827 |
| MluCI | AATT | 2 cut(s) | 331, 837 |
| MmeI | TCCRAC | 3 cut(s) | 760, 769, 778 |
| MnlI | CCTC | 5 cut(s) | 465, 621, 693, 773, 800 |
| Mph1103I | ATGCAT | 5 cut(s) | 189, 345, 355, 417, 578 |
| MseI | TTAA | 5 cut(s) | 270, 368, 464, 471, 582 |
| MslI | CAYNNNNRTG | 5 cut(s) | 104, 207, 325, 348, 575 |
| MspA1I | CMGCKG | 1 cut(s) | 446 |
| Mva1269I | GAATGC | 3 cut(s) | 187, 320, 343 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 336, 359, 421 |
| NmuCI | GTSAC | 1 cut(s) | 97 |
| NsiI | ATGCAT | 5 cut(s) | 189, 345, 355, 417, 578 |
| PaeI | GCATGC | 3 cut(s) | 343, 517, 580 |
| PctI | GAATGC | 3 cut(s) | 187, 320, 343 |
| PkrI | GCNGC | 2 cut(s) | 445, 448 |
| PmaCI | CACGTG | 1 cut(s) | 478 |
| PmlI | CACGTG | 1 cut(s) | 478 |
| Ppu21I | YACGTR | 1 cut(s) | 478 |
| Psp1406I | AACGTT | 1 cut(s) | 828 |
| PspCI | CACGTG | 1 cut(s) | 478 |
| PvuII | CAGCTG | 1 cut(s) | 446 |
| RseI | CAYNNNNRTG | 5 cut(s) | 104, 207, 325, 348, 575 |
| SaqAI | TTAA | 5 cut(s) | 270, 368, 464, 471, 582 |
| SatI | GCNGC | 2 cut(s) | 444, 447 |
| SetI | ASST | 8 cut(s) | 93, 408, 448, 480, 605, 785, 792, 831 |
| SfaNI | GCATC | 4 cut(s) | 63, 458, 638, 708 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 104, 207, 325, 348, 575 |
| SphI | GCATGC | 3 cut(s) | 343, 517, 580 |
| Sse9I | AATT | 2 cut(s) | 331, 837 |
| SspMI | CTAG | 2 cut(s) | 39, 92 |
| StyI | CCWWGG | 1 cut(s) | 508 |
| TaaI | ACNGT | 1 cut(s) | 549 |
| TaiI | ACGT | 2 cut(s) | 480, 831 |
| TasI | AATT | 2 cut(s) | 331, 837 |
| Tru1I | TTAA | 5 cut(s) | 270, 368, 464, 471, 582 |
| Tru9I | TTAA | 5 cut(s) | 270, 368, 464, 471, 582 |
| TscAI | CASTG | 1 cut(s) | 437 |
| TseFI | GTSAC | 1 cut(s) | 97 |
| TseI | GCWGC | 2 cut(s) | 443, 446 |
| Tsp45I | GTSAC | 1 cut(s) | 97 |
| TspRI | CASTG | 1 cut(s) | 437 |
| XspI | CTAG | 2 cut(s) | 39, 92 |
| Zsp2I | ATGCAT | 5 cut(s) | 189, 345, 355, 417, 578 |