pycom889g00050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000889
OG0001078
Physical Location & Seq
Forward (+)
56306 .. 57456
1151 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom889g00050.2

Sequence Viewer

Length: 960 bp
ATGGCTTGTTCTCGTGCATTTACGTTATGTTTCTCTCGTTATTTTAGTCCTTTATGCTTCTTTTGTGTGTTTTCAGGTTCTAAGGGCCTAGGGAGCAAGAAAGTGCATTTTGAGGCCATTTGGAGCATTTTTGGGCATGGATTGGATAGCTTATCCATGGAGCCAAGAGGATGGACGAATTTGAAGGCCTTGTATGCACTTGAAGACACAAAACAATGTTTAGTTATCACTTATCAAACCATTCACTTATCACTTAACATATCATTCATTTATTTCACTTCCATACTCCTCTTAATTCCACATGCATTCTCACACATGCACATCATTCACTCACATTTCCACCATTCATTCTTTATTCCTTTCCAAACATCTCACATTCATCATAATTCACAACACAAAACAACTTCATTGCCGTGGGTTCCCATTTCCTTTCCAAACATCTCACATGCATTTCTTCATTATTTCCTAACCCTACATGCATTATTTATTGCATCTTCACATGCATTCACACACACTTTACACATTCAAACCGTGAGCTCCCATTCCTATATGCACTTCCACCTTTCATCCACAATGATTCATGATTCATTCACTTTGCATCCTTTAATTCAATCACATACTTTTCCATCATTAATTAGCCACTTGCATCTTAAACAAACAGCCATATGTTCCCTCCACTACTCCTATAAATACCCTTGCATTCATTCATTCAATTCATATCTCATATTTCCATTCACAACACTTCACACAACACAAATACACATCCATTGCCGCAATTCCCCTTCCATTTTCTGTGCAGTTTTTCTCCTTCATTGCAGCCACTATCCAACCACTTCCCATCCCTCCAAAACACTTCACATAATCCCCAAAGCTCACCTTAGACCTTGTGCTACAACAACGAGGAAGAAAAGAGTGCCTAAACGTTCATACAATTCAAGTTTGAGTTGTTGGAATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

320

Amino Acids

36.78

Weight (kDa)

9.43

Isoelectric Point (pI)

56.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 772
AclI AACGTT 1 cut(s) 922
AcsI RAATTY 1 cut(s) 178
AgsI TTSAA 6 cut(s) 184, 203, 527, 611, 712, 936
AluBI AGCT 3 cut(s) 150, 537, 872
AluI AGCT 3 cut(s) 150, 537, 872
Alw21I GWGCWC 1 cut(s) 539
AoxI GGCC 3 cut(s) 85, 114, 186
ApeKI GCWGC 1 cut(s) 816
ApoI RAATTY 1 cut(s) 178
AseI ATTAAT 1 cut(s) 632
AspA2I CCTAGG 1 cut(s) 88
AspS9I GGNCC 1 cut(s) 85
AsuHPI GGTGA 1 cut(s) 866
AvrII CCTAGG 1 cut(s) 88
BanII GRGCYC 1 cut(s) 539
BauI CACGAG 1 cut(s) 12
BbsI GAAGAC 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 539
BbvI GCAGC 1 cut(s) 828
BccI CCATC 3 cut(s) 165, 634, 846
BceAI ACGGC 1 cut(s) 397
BfaI CTAG 1 cut(s) 89
BisI GCNGC 2 cut(s) 772, 817
BlnI CCTAGG 1 cut(s) 88
BlsI GCNGC 2 cut(s) 773, 818
BmgT120I GGNCC 1 cut(s) 85
BmiI GGNNCC 2 cut(s) 162, 420
BmsI GCATC 3 cut(s) 500, 607, 655
BpiI GAAGAC 1 cut(s) 210
BsaJI CCNNGG 3 cut(s) 88, 156, 413
Bse3DI GCAATG 3 cut(s) 407, 766, 811
BseDI CCNNGG 3 cut(s) 88, 156, 413
BseGI GGATG 5 cut(s) 176, 566, 598, 762, 838
BseMI GCAATG 3 cut(s) 407, 766, 811
BseRI GAGGAG 1 cut(s) 278
BseXI GCAGC 1 cut(s) 828
BsgI GTGCAG 1 cut(s) 816
BshFI GGCC 3 cut(s) 87, 116, 188
BsiHKAI GWGCWC 1 cut(s) 539
BsmI GAATGC 3 cut(s) 305, 503, 699
BsnI GGCC 3 cut(s) 87, 116, 188
Bsp1286I GDGCHC 1 cut(s) 539
Bsp19I CCATGG 1 cut(s) 156
BspACI CCGC 1 cut(s) 772
BspANI GGCC 3 cut(s) 87, 116, 188
BspHI TCATGA 1 cut(s) 580
BspLI GGNNCC 2 cut(s) 162, 420
BsrDI GCAATG 3 cut(s) 407, 766, 811
BssECI CCNNGG 3 cut(s) 88, 156, 413
BssSI CACGAG 1 cut(s) 12
BssT1I CCWWGG 2 cut(s) 88, 156
Bst2BI CACGAG 1 cut(s) 12
Bst4CI ACNGT 1 cut(s) 532
BstDEI CTNAG 2 cut(s) 81, 878
BstDSI CCRYGG 2 cut(s) 156, 413
BstF5I GGATG 5 cut(s) 176, 566, 598, 762, 838
BstMWI GCNNNNNNNGC 2 cut(s) 93, 194
BstNSI RCATGY 5 cut(s) 305, 319, 449, 479, 503
BstV1I GCAGC 1 cut(s) 828
BstV2I GAAGAC 1 cut(s) 210
BstXI CCANNNNNNTGG 1 cut(s) 171
BsuRI GGCC 3 cut(s) 87, 116, 188
BtgI CCRYGG 2 cut(s) 156, 413
BtsCI GGATG 5 cut(s) 176, 566, 598, 762, 838
CciI TCATGA 1 cut(s) 580
Cfr13I GGNCC 1 cut(s) 85
CviAII CATG 8 cut(s) 137, 157, 302, 316, 446, 476, 500, 581
DdeI CTNAG 2 cut(s) 81, 878
Ecl136II GAGCTC 1 cut(s) 537
Eco130I CCWWGG 2 cut(s) 88, 156
Eco147I AGGCCT 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 539
Eco53kI GAGCTC 1 cut(s) 537
EcoICRI GAGCTC 1 cut(s) 537
EcoO109I RGGNCCY 1 cut(s) 85
EcoT14I CCWWGG 2 cut(s) 88, 156
EcoT22I ATGCAT 4 cut(s) 307, 451, 481, 505
EcoT38I GRGCYC 1 cut(s) 539
ErhI CCWWGG 2 cut(s) 88, 156
FaeI CATG 8 cut(s) 140, 160, 305, 319, 449, 479, 503, 584
FalI AAGNNNNNCTT 2 cut(s) 861, 893
FatI CATG 8 cut(s) 136, 156, 301, 315, 445, 475, 499, 580
FauNDI CATATG 1 cut(s) 665
Fnu4HI GCNGC 2 cut(s) 772, 817
FokI GGATG 5 cut(s) 183, 553, 585, 749, 825
FriOI GRGCYC 1 cut(s) 539
Fsp4HI GCNGC 2 cut(s) 772, 817
FspBI CTAG 1 cut(s) 89
GluI GCNGC 2 cut(s) 772, 817
HaeIII GGCC 3 cut(s) 87, 116, 188
Hin1II CATG 8 cut(s) 140, 160, 305, 319, 449, 479, 503, 584
HinfI GANTC 2 cut(s) 577, 584
HphI GGTGA 1 cut(s) 866
Hpy188III TCNNGA 1 cut(s) 581
HpyAV CCTTC 3 cut(s) 178, 792, 818
HpyCH4III ACNGT 1 cut(s) 532
HpyCH4IV ACGT 2 cut(s) 23, 922
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 194
HpyF3I CTNAG 2 cut(s) 81, 878
HpySE526I ACGT 2 cut(s) 23, 922
Hsp92II CATG 8 cut(s) 140, 160, 305, 319, 449, 479, 503, 584
LmnI GCTCC 4 cut(s) 93, 123, 160, 542
LpnPI CCDG 1 cut(s) 60
Lsp1109I GCAGC 1 cut(s) 828
LweI GCATC 3 cut(s) 500, 607, 655
MaeI CTAG 1 cut(s) 89
MaeII ACGT 2 cut(s) 23, 922
MboII GAAGA 4 cut(s) 215, 446, 486, 916
MhlI GDGCHC 1 cut(s) 539
MluCI AATT 8 cut(s) 178, 294, 385, 606, 633, 712, 775, 931
MmeI TCCRAC 2 cut(s) 851, 929
MnlI CCTC 6 cut(s) 106, 161, 299, 683, 853, 894
Mph1103I ATGCAT 4 cut(s) 307, 451, 481, 505
MseI TTAA 5 cut(s) 255, 293, 605, 632, 651
MslI CAYNNNNRTG 1 cut(s) 412
Mva1269I GAATGC 3 cut(s) 305, 503, 699
MwoI GCNNNNNNNGC 2 cut(s) 93, 194
NcoI CCATGG 1 cut(s) 156
NdeI CATATG 1 cut(s) 665
NlaIII CATG 8 cut(s) 140, 160, 305, 319, 449, 479, 503, 584
NlaIV GGNNCC 2 cut(s) 162, 420
NsiI ATGCAT 4 cut(s) 307, 451, 481, 505
NspI RCATGY 5 cut(s) 305, 319, 449, 479, 503
PagI TCATGA 1 cut(s) 580
PceI AGGCCT 1 cut(s) 188
PctI GAATGC 3 cut(s) 305, 503, 699
PfeI GAWTC 2 cut(s) 577, 584
PkrI GCNGC 2 cut(s) 773, 818
PshBI ATTAAT 1 cut(s) 632
Psp124BI GAGCTC 1 cut(s) 539
Psp1406I AACGTT 1 cut(s) 922
PspN4I GGNNCC 2 cut(s) 162, 420
PspPI GGNCC 1 cut(s) 85
RseI CAYNNNNRTG 1 cut(s) 412
SacI GAGCTC 1 cut(s) 539
SaqAI TTAA 5 cut(s) 255, 293, 605, 632, 651
SatI GCNGC 2 cut(s) 772, 817
Sau96I GGNCC 1 cut(s) 85
SduI GDGCHC 1 cut(s) 539
SetI ASST 9 cut(s) 26, 79, 152, 539, 564, 874, 879, 886, 925
SfaNI GCATC 3 cut(s) 500, 607, 655
SmiMI CAYNNNNRTG 1 cut(s) 412
Sse9I AATT 8 cut(s) 178, 294, 385, 606, 633, 712, 775, 931
SseBI AGGCCT 1 cut(s) 188
SsiI CCGC 1 cut(s) 772
SspMI CTAG 1 cut(s) 89
SstI GAGCTC 1 cut(s) 539
StuI AGGCCT 1 cut(s) 188
StyI CCWWGG 2 cut(s) 88, 156
TaaI ACNGT 1 cut(s) 532
TaiI ACGT 2 cut(s) 26, 925
TasI AATT 8 cut(s) 178, 294, 385, 606, 633, 712, 775, 931
TauI GCSGC 1 cut(s) 774
TfiI GAWTC 2 cut(s) 577, 584
Tru1I TTAA 5 cut(s) 255, 293, 605, 632, 651
Tru9I TTAA 5 cut(s) 255, 293, 605, 632, 651
TseI GCWGC 1 cut(s) 816
VspI ATTAAT 1 cut(s) 632
XapI RAATTY 1 cut(s) 178
XceI RCATGY 5 cut(s) 305, 319, 449, 479, 503
XmaJI CCTAGG 1 cut(s) 88
XspI CTAG 1 cut(s) 89
Zsp2I ATGCAT 4 cut(s) 307, 451, 481, 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.