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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclI | AACGTT | 1 cut(s) | 406 |
| AcsI | RAATTY | 2 cut(s) | 184, 254 |
| AgsI | TTSAA | 2 cut(s) | 139, 420 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 285, 317 |
| AluBI | AGCT | 1 cut(s) | 84 |
| AluI | AGCT | 1 cut(s) | 84 |
| AoxI | GGCC | 1 cut(s) | 398 |
| ApeKI | GCWGC | 4 cut(s) | 127, 282, 285, 315 |
| ApoI | RAATTY | 2 cut(s) | 184, 254 |
| AspS9I | GGNCC | 1 cut(s) | 398 |
| AsuHPI | GGTGA | 2 cut(s) | 46, 96 |
| BbvI | GCAGC | 4 cut(s) | 139, 294, 297, 327 |
| BccI | CCATC | 3 cut(s) | 59, 217, 357 |
| BfaI | CTAG | 1 cut(s) | 81 |
| BisI | GCNGC | 4 cut(s) | 128, 283, 286, 316 |
| BlsI | GCNGC | 4 cut(s) | 129, 284, 287, 317 |
| BmgT120I | GGNCC | 1 cut(s) | 398 |
| BmsI | GCATC | 2 cut(s) | 53, 127 |
| Bse3DI | GCAATG | 2 cut(s) | 122, 310 |
| BseGI | GGATG | 3 cut(s) | 118, 188, 209 |
| BseMI | GCAATG | 2 cut(s) | 122, 310 |
| BseRI | GAGGAG | 1 cut(s) | 296 |
| BseXI | GCAGC | 4 cut(s) | 139, 294, 297, 327 |
| BshFI | GGCC | 1 cut(s) | 400 |
| BsmI | GAATGC | 1 cut(s) | 142 |
| BsnI | GGCC | 1 cut(s) | 400 |
| BspANI | GGCC | 1 cut(s) | 400 |
| BsrDI | GCAATG | 2 cut(s) | 122, 310 |
| Bst6I | CTCTTC | 3 cut(s) | 176, 244, 377 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 124 |
| BstDEI | CTNAG | 1 cut(s) | 362 |
| BstF5I | GGATG | 3 cut(s) | 118, 188, 209 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 124 |
| BstNSI | RCATGY | 2 cut(s) | 27, 44 |
| BstV1I | GCAGC | 4 cut(s) | 139, 294, 297, 327 |
| BsuRI | GGCC | 1 cut(s) | 400 |
| BtsCI | GGATG | 3 cut(s) | 118, 188, 209 |
| Cfr13I | GGNCC | 1 cut(s) | 398 |
| CviAII | CATG | 3 cut(s) | 24, 41, 89 |
| CviJI | RGCY | 6 cut(s) | 37, 84, 130, 288, 318, 400 |
| CviKI_1 | RGCY | 6 cut(s) | 37, 84, 130, 288, 318, 400 |
| DdeI | CTNAG | 1 cut(s) | 362 |
| Eam1104I | CTCTTC | 3 cut(s) | 176, 244, 377 |
| EarI | CTCTTC | 3 cut(s) | 176, 244, 377 |
| EcoO109I | RGGNCCY | 1 cut(s) | 398 |
| EcoT22I | ATGCAT | 3 cut(s) | 6, 29, 46 |
| FaeI | CATG | 3 cut(s) | 27, 44, 92 |
| FaiI | YATR | 7 cut(s) | 25, 29, 42, 90, 110, 245, 412 |
| FatI | CATG | 3 cut(s) | 23, 40, 88 |
| Fnu4HI | GCNGC | 4 cut(s) | 128, 283, 286, 316 |
| FokI | GGATG | 3 cut(s) | 105, 175, 196 |
| Fsp4HI | GCNGC | 4 cut(s) | 128, 283, 286, 316 |
| FspBI | CTAG | 1 cut(s) | 81 |
| GluI | GCNGC | 4 cut(s) | 128, 283, 286, 316 |
| HaeIII | GGCC | 1 cut(s) | 400 |
| Hin1II | CATG | 3 cut(s) | 27, 44, 92 |
| HphI | GGTGA | 2 cut(s) | 46, 96 |
| Hpy188III | TCNNGA | 2 cut(s) | 62, 250 |
| HpyCH4IV | ACGT | 1 cut(s) | 406 |
| HpyCH4V | TGCA | 6 cut(s) | 4, 27, 44, 127, 282, 315 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 124 |
| HpyF3I | CTNAG | 1 cut(s) | 362 |
| HpySE526I | ACGT | 1 cut(s) | 406 |
| Hsp92II | CATG | 3 cut(s) | 27, 44, 92 |
| LpnPI | CCDG | 2 cut(s) | 75, 263 |
| Lsp1109I | GCAGC | 4 cut(s) | 139, 294, 297, 327 |
| LweI | GCATC | 2 cut(s) | 53, 127 |
| MaeI | CTAG | 1 cut(s) | 81 |
| MaeII | ACGT | 1 cut(s) | 406 |
| MboII | GAAGA | 4 cut(s) | 163, 231, 394, 400 |
| MluCI | AATT | 5 cut(s) | 134, 184, 254, 354, 415 |
| MmeI | TCCRAC | 2 cut(s) | 348, 413 |
| MnlI | CCTC | 5 cut(s) | 234, 277, 317, 378, 389 |
| Mph1103I | ATGCAT | 3 cut(s) | 6, 29, 46 |
| MseI | TTAA | 2 cut(s) | 17, 216 |
| Mva1269I | GAATGC | 1 cut(s) | 142 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 124 |
| NlaIII | CATG | 3 cut(s) | 27, 44, 92 |
| NsiI | ATGCAT | 3 cut(s) | 6, 29, 46 |
| NspI | RCATGY | 2 cut(s) | 27, 44 |
| PctI | GAATGC | 1 cut(s) | 142 |
| PkrI | GCNGC | 4 cut(s) | 129, 284, 287, 317 |
| Psp1406I | AACGTT | 1 cut(s) | 406 |
| PspPI | GGNCC | 1 cut(s) | 398 |
| SaqAI | TTAA | 2 cut(s) | 17, 216 |
| SatI | GCNGC | 4 cut(s) | 128, 283, 286, 316 |
| Sau96I | GGNCC | 1 cut(s) | 398 |
| SetI | ASST | 6 cut(s) | 86, 109, 226, 269, 370, 409 |
| SfaNI | GCATC | 2 cut(s) | 53, 127 |
| Sse9I | AATT | 5 cut(s) | 134, 184, 254, 354, 415 |
| SspMI | CTAG | 1 cut(s) | 81 |
| TaiI | ACGT | 1 cut(s) | 409 |
| TasI | AATT | 5 cut(s) | 134, 184, 254, 354, 415 |
| Tru1I | TTAA | 2 cut(s) | 17, 216 |
| Tru9I | TTAA | 2 cut(s) | 17, 216 |
| TseI | GCWGC | 4 cut(s) | 127, 282, 285, 315 |
| TspDTI | ATGAA | 2 cut(s) | 177, 399 |
| XapI | RAATTY | 2 cut(s) | 184, 254 |
| XceI | RCATGY | 2 cut(s) | 27, 44 |
| XspI | CTAG | 1 cut(s) | 81 |
| Zsp2I | ATGCAT | 3 cut(s) | 6, 29, 46 |