RchiOBHm_Chr3g0486651

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000981
Physical Location & Seq
Reverse (-)
33504865 .. 33506027
1163 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45114

Sequence Viewer

Length: 384 bp
ATGAAGTTCTCTCTTCTCTCTAGAGCAACCCTAAGGTTGCAACTGACTTTGATTGGCGGCAGCGGAGCTACAAACTCTCTGACTCTTGGCAGAGAGGCTTCCCACCTACGTAGTGGTTCTGAACCTTGGATTTGGAAGGGCGCCGACAACTCTCTTTCTCCTCCCCTCCTTGGTAGCGACGGTGATTGGCTTCGACGCCCCTTGAATTTATGTCTGGATCAGTTTGTCTTCTTATGCCTGGAAGCCTCTAATGGCAGTCATCTTGGCTGCAATAGAGTGAGTTTCCTCGTCCTCATCCTGTGGGATGGGGATTTGGTGATGGCTATGTTGCTTAGGTCGACTTGGGTTCAGATCAGATCAAATGGGATCGATCGGCTCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

127

Amino Acids

14.02

Weight (kDa)

8.66

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 140
AccI GTMKAC 1 cut(s) 338
AciI CCGC 2 cut(s) 57, 63
AclWI GGATC 2 cut(s) 225, 374
AcsI RAATTY 1 cut(s) 205
AcyI GRCGYC 2 cut(s) 141, 196
AfiI CCNNNNNNNGG 1 cut(s) 170
AgsI TTSAA 1 cut(s) 205
AjnI CCWGG 1 cut(s) 237
AluBI AGCT 1 cut(s) 68
AluI AGCT 1 cut(s) 68
AlwI GGATC 2 cut(s) 225, 374
ApeKI GCWGC 2 cut(s) 60, 267
ApoI RAATTY 1 cut(s) 205
AspLEI GCGC 1 cut(s) 143
AsuHPI GGTGA 2 cut(s) 194, 328
AxyI CCTNAGG 1 cut(s) 32
BanI GGYRCC 1 cut(s) 140
BbsI GAAGAC 1 cut(s) 220
BbvI GCAGC 2 cut(s) 72, 254
BccI CCATC 2 cut(s) 299, 313
BciT130I CCWGG 1 cut(s) 239
BfaI CTAG 1 cut(s) 21
BfoI RGCGCY 1 cut(s) 144
BisI GCNGC 3 cut(s) 58, 61, 268
BlsI GCNGC 3 cut(s) 59, 62, 269
Bme1390I CCNGG 1 cut(s) 239
BmiI GGNNCC 1 cut(s) 142
BmrFI CCNGG 1 cut(s) 239
BpiI GAAGAC 1 cut(s) 220
Bpu10I CCTNAGC 1 cut(s) 332
Bsa29I ATCGAT 1 cut(s) 369
BsaAI YACGTR 1 cut(s) 110
BsaHI GRCGYC 2 cut(s) 141, 196
BsaJI CCNNGG 2 cut(s) 125, 169
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse21I CCTNAGG 1 cut(s) 32
BseBI CCWGG 1 cut(s) 239
BseCI ATCGAT 1 cut(s) 369
BseDI CCNNGG 2 cut(s) 125, 169
BseGI GGATG 2 cut(s) 294, 310
BseLI CCNNNNNNNGG 1 cut(s) 170
BseRI GAGGAG 1 cut(s) 150
BseXI GCAGC 2 cut(s) 72, 254
Bsh1285I CGRYCG 1 cut(s) 373
BshNI GGYRCC 1 cut(s) 140
BshVI ATCGAT 1 cut(s) 369
BsiEI CGRYCG 1 cut(s) 373
BslI CCNNNNNNNGG 1 cut(s) 170
Bsp143I GATC 5 cut(s) 217, 351, 356, 366, 370
BspACI CCGC 2 cut(s) 57, 63
BspDI ATCGAT 1 cut(s) 369
BspLI GGNNCC 1 cut(s) 142
BspPI GGATC 2 cut(s) 225, 374
BspT107I GGYRCC 1 cut(s) 140
BssECI CCNNGG 2 cut(s) 125, 169
BssMI GATC 5 cut(s) 217, 351, 356, 366, 370
BssNI GRCGYC 2 cut(s) 141, 196
BssT1I CCWWGG 2 cut(s) 125, 169
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 1 cut(s) 182
Bst6I CTCTTC 1 cut(s) 18
BstACI GRCGYC 2 cut(s) 141, 196
BstBAI YACGTR 1 cut(s) 110
BstDEI CTNAG 2 cut(s) 32, 332
BstF5I GGATG 2 cut(s) 294, 310
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 5 cut(s) 220, 354, 359, 369, 373
BstMBI GATC 5 cut(s) 217, 351, 356, 366, 370
BstMCI CGRYCG 1 cut(s) 373
BstNI CCWGG 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 237
BstSNI TACGTA 1 cut(s) 110
BstV1I GCAGC 2 cut(s) 72, 254
BstV2I GAAGAC 1 cut(s) 220
Bsu15I ATCGAT 1 cut(s) 369
Bsu36I CCTNAGG 1 cut(s) 32
BsuTUI ATCGAT 1 cut(s) 369
BtsCI GGATG 2 cut(s) 294, 310
CfoI GCGC 1 cut(s) 143
ClaI ATCGAT 1 cut(s) 369
CseI GACGC 1 cut(s) 204
CviJI RGCY 7 cut(s) 68, 98, 190, 245, 267, 323, 376
CviKI_1 RGCY 7 cut(s) 68, 98, 190, 245, 267, 323, 376
DdeI CTNAG 2 cut(s) 32, 332
DinI GGCGCC 1 cut(s) 142
DpnI GATC 5 cut(s) 219, 353, 358, 368, 372
DpnII GATC 5 cut(s) 217, 351, 356, 366, 370
Eam1104I CTCTTC 1 cut(s) 18
EarI CTCTTC 1 cut(s) 18
Eco105I TACGTA 1 cut(s) 110
Eco130I CCWWGG 2 cut(s) 125, 169
Eco81I CCTNAGG 1 cut(s) 32
EcoRII CCWGG 1 cut(s) 237
EcoT14I CCWWGG 2 cut(s) 125, 169
EgeI GGCGCC 1 cut(s) 142
EheI GGCGCC 1 cut(s) 142
ErhI CCWWGG 2 cut(s) 125, 169
FaiI YATR 4 cut(s) 211, 235, 326, 382
FblI GTMKAC 1 cut(s) 338
Fnu4HI GCNGC 3 cut(s) 58, 61, 268
FokI GGATG 2 cut(s) 281, 317
Fsp4HI GCNGC 3 cut(s) 58, 61, 268
FspBI CTAG 1 cut(s) 21
GlaI GCGC 1 cut(s) 142
GluI GCNGC 3 cut(s) 58, 61, 268
HaeII RGCGCY 1 cut(s) 144
HgaI GACGC 1 cut(s) 204
HhaI GCGC 1 cut(s) 143
Hin1I GRCGYC 2 cut(s) 141, 196
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HincII GTYRAC 1 cut(s) 339
HindII GTYRAC 1 cut(s) 339
HinfI GANTC 1 cut(s) 82
HphI GGTGA 2 cut(s) 194, 328
Hpy166II GTNNAC 1 cut(s) 339
Hpy188I TCNGA 4 cut(s) 81, 121, 351, 356
Hpy188III TCNNGA 2 cut(s) 21, 215
Hpy8I GTNNAC 1 cut(s) 339
Hpy99I CGWCG 2 cut(s) 182, 198
HpyAV CCTTC 1 cut(s) 130
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4IV ACGT 1 cut(s) 109
HpyCH4V TGCA 2 cut(s) 40, 270
HpyF3I CTNAG 2 cut(s) 32, 332
HpySE526I ACGT 1 cut(s) 109
Hsp92I GRCGYC 2 cut(s) 141, 196
HspAI GCGC 1 cut(s) 141
KasI GGCGCC 1 cut(s) 140
Kzo9I GATC 5 cut(s) 217, 351, 356, 366, 370
LmnI GCTCC 1 cut(s) 65
LpnPI CCDG 4 cut(s) 200, 224, 251, 311
Lsp1109I GCAGC 2 cut(s) 72, 254
MaeI CTAG 1 cut(s) 21
MaeII ACGT 1 cut(s) 109
MalI GATC 5 cut(s) 219, 353, 358, 368, 372
MboI GATC 5 cut(s) 217, 351, 356, 366, 370
MboII GAAGA 2 cut(s) 5, 220
MluCI AATT 1 cut(s) 205
Mly113I GGCGCC 1 cut(s) 141
MlyI GAGTC 1 cut(s) 76
MnlI CCTC 6 cut(s) 88, 171, 176, 256, 296, 302
MspA1I CMGCKG 1 cut(s) 63
MspR9I CCNGG 1 cut(s) 239
MvaI CCWGG 1 cut(s) 239
NarI GGCGCC 1 cut(s) 141
NdeII GATC 5 cut(s) 217, 351, 356, 366, 370
NlaIV GGNNCC 1 cut(s) 142
PkrI GCNGC 3 cut(s) 59, 62, 269
Ple19I CGATCG 1 cut(s) 373
PleI GAGTC 1 cut(s) 76
PluTI GGCGCC 1 cut(s) 144
PpsI GAGTC 1 cut(s) 76
Ppu21I YACGTR 1 cut(s) 110
Psp6I CCWGG 1 cut(s) 237
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 1 cut(s) 142
PvuI CGATCG 1 cut(s) 373
SalI GTCGAC 1 cut(s) 337
SatI GCNGC 3 cut(s) 58, 61, 268
Sau3AI GATC 5 cut(s) 217, 351, 356, 366, 370
SchI GAGTC 1 cut(s) 76
ScrFI CCNGG 1 cut(s) 239
SetI ASST 6 cut(s) 38, 70, 108, 112, 127, 338
SfoI GGCGCC 1 cut(s) 142
SnaBI TACGTA 1 cut(s) 110
Sse9I AATT 1 cut(s) 205
SsiI CCGC 2 cut(s) 57, 63
SspDI GGCGCC 1 cut(s) 140
SspMI CTAG 1 cut(s) 21
StyD4I CCNGG 1 cut(s) 237
StyI CCWWGG 2 cut(s) 125, 169
TaaI ACNGT 1 cut(s) 182
TaiI ACGT 1 cut(s) 112
TaqI TCGA 3 cut(s) 193, 338, 369
TasI AATT 1 cut(s) 205
TauI GCSGC 1 cut(s) 60
TseI GCWGC 2 cut(s) 60, 267
TspDTI ATGAA 1 cut(s) 17
XapI RAATTY 1 cut(s) 205
XbaI TCTAGA 1 cut(s) 20
XcmI CCANNNNNNNNNTGG 1 cut(s) 110
XmiI GTMKAC 1 cut(s) 338
XspI CTAG 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.