RLG00000011512

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000981
Physical Location & Seq
Reverse (-)
10086052 .. 10086594
543 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011512

Sequence Viewer

Length: 372 bp
ATGGATGGCTCTGCTGCTCTTTCTACTCATCTCCTTGACAGCAACGGCGATTGGATTCCACGGTGCTCTTCAAGTTTCGATCGGGTTTGTCTCCTTGGGGGATCGAAGCCTCAGATGATGGCCATTTGGGCCATGATAGTGCGTGGCTCCTCGCCTTCAATCTTGTCAGTGACAAGCGGATCAGAGGCAACCGGTGACTGGGTTTGCTCGAGGCCGGCTTTGATGAGCGACAATGGTTTTGCAGGTTTGGGTCTCTGGTGTTTCCCTGCCGGCAAAGGTGGTGGCGGATCCATGGTGGCGGCAGTTTGTGGTGGTGGCTTACCCATGATCACAACGGCGGCAACACTATGGGGGAGACTTTTTCTCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

124

Amino Acids

12.56

Weight (kDa)

6.51

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 233
AciI CCGC 4 cut(s) 177, 285, 299, 338
AclWI GGATC 4 cut(s) 109, 187, 282, 295
AcoI YGGCCR 1 cut(s) 120
AfiI CCNNNNNNNGG 1 cut(s) 198
AgeI ACCGGT 1 cut(s) 191
AgsI TTSAA 2 cut(s) 72, 159
Alw21I GWGCWC 1 cut(s) 68
Alw26I GTCTC 3 cut(s) 95, 257, 349
AlwI GGATC 4 cut(s) 109, 187, 282, 295
Ama87I CYCGRG 1 cut(s) 208
AoxI GGCC 3 cut(s) 120, 129, 212
ApeKI GCWGC 1 cut(s) 14
ArsI GACNNNNNNTTYG 2 cut(s) 221, 253
AsiGI ACCGGT 1 cut(s) 191
AspS9I GGNCC 1 cut(s) 129
AsuHPI GGTGA 1 cut(s) 206
AvaI CYCGRG 1 cut(s) 208
BalI TGGCCA 1 cut(s) 122
BamHI GGATCC 1 cut(s) 287
Bbv12I GWGCWC 1 cut(s) 68
BccI CCATC 1 cut(s) 112
BceAI ACGGC 2 cut(s) 61, 351
BclI TGATCA 1 cut(s) 327
BcoDI GTCTC 3 cut(s) 95, 257, 349
BfaI CTAG 1 cut(s) 370
BfuAI ACCTGC 1 cut(s) 233
BglI GCCNNNNNGGC 1 cut(s) 128
BisI GCNGC 3 cut(s) 15, 300, 339
BlsI GCNGC 3 cut(s) 16, 301, 340
BmeT110I CYCGRG 1 cut(s) 208
BmgT120I GGNCC 1 cut(s) 129
BmiI GGNNCC 2 cut(s) 148, 289
BmrI ACTGGG 1 cut(s) 208
BmuI ACTGGG 1 cut(s) 208
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 3 cut(s) 59, 94, 291
BsaWI WCCGGW 1 cut(s) 191
Bsc4I CCNNNNNNNGG 1 cut(s) 198
Bse118I RCCGGY 3 cut(s) 191, 214, 269
Bse1I ACTGG 1 cut(s) 203
BseDI CCNNGG 3 cut(s) 59, 94, 291
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 1 cut(s) 198
BseMII CTCAG 1 cut(s) 125
BseNI ACTGG 1 cut(s) 203
BseRI GAGGAG 1 cut(s) 139
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 3 cut(s) 122, 131, 214
BshTI ACCGGT 1 cut(s) 191
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 68
BsiHKCI CYCGRG 1 cut(s) 208
BsiSI CCGG 3 cut(s) 192, 215, 270
BslI CCNNNNNNNGG 1 cut(s) 198
BsmAI GTCTC 3 cut(s) 95, 257, 349
BsnI GGCC 3 cut(s) 122, 131, 214
Bso31I GGTCTC 1 cut(s) 257
BsoBI CYCGRG 1 cut(s) 208
Bsp1286I GDGCHC 1 cut(s) 68
Bsp143I GATC 5 cut(s) 79, 101, 179, 287, 327
Bsp19I CCATGG 1 cut(s) 291
BspACI CCGC 4 cut(s) 177, 285, 299, 338
BspANI GGCC 3 cut(s) 122, 131, 214
BspCNI CTCAG 1 cut(s) 124
BspLI GGNNCC 2 cut(s) 148, 289
BspMI ACCTGC 1 cut(s) 233
BspPI GGATC 4 cut(s) 109, 187, 282, 295
BspQI GCTCTTC 1 cut(s) 73
BspTNI GGTCTC 1 cut(s) 257
BsrFI RCCGGY 3 cut(s) 191, 214, 269
BsrI ACTGG 1 cut(s) 203
BssAI RCCGGY 3 cut(s) 191, 214, 269
BssECI CCNNGG 3 cut(s) 59, 94, 291
BssMI GATC 5 cut(s) 79, 101, 179, 287, 327
BssT1I CCWWGG 2 cut(s) 94, 291
Bst4CI ACNGT 1 cut(s) 63
Bst6I CTCTTC 1 cut(s) 73
BstC8I GCNNGC 2 cut(s) 216, 271
BstDEI CTNAG 1 cut(s) 111
BstDSI CCRYGG 2 cut(s) 59, 291
BstF5I GGATG 1 cut(s) 10
BstKTI GATC 5 cut(s) 82, 104, 182, 290, 330
BstMAI GTCTC 3 cut(s) 95, 257, 349
BstMBI GATC 5 cut(s) 79, 101, 179, 287, 327
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstX2I RGATCY 1 cut(s) 287
BstYI RGATCY 1 cut(s) 287
BsuRI GGCC 3 cut(s) 122, 131, 214
BtgI CCRYGG 2 cut(s) 59, 291
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 1 cut(s) 174
BveI ACCTGC 1 cut(s) 233
Cac8I GCNNGC 2 cut(s) 216, 271
Cfr10I RCCGGY 3 cut(s) 191, 214, 269
Cfr13I GGNCC 1 cut(s) 129
CspAI ACCGGT 1 cut(s) 191
CspCI CAANNNNNGTGG 2 cut(s) 262, 297
CviAII CATG 3 cut(s) 133, 292, 325
CviJI RGCY 8 cut(s) 9, 109, 122, 131, 147, 214, 218, 318
CviKI_1 RGCY 8 cut(s) 9, 109, 122, 131, 147, 214, 218, 318
DdeI CTNAG 1 cut(s) 111
DpnI GATC 5 cut(s) 81, 103, 181, 289, 329
DpnII GATC 5 cut(s) 79, 101, 179, 287, 327
EaeI YGGCCR 1 cut(s) 120
Eam1104I CTCTTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 73
EciI GGCGGA 1 cut(s) 300
Eco130I CCWWGG 2 cut(s) 94, 291
Eco31I GGTCTC 1 cut(s) 257
Eco88I CYCGRG 1 cut(s) 208
EcoT14I CCWWGG 2 cut(s) 94, 291
ErhI CCWWGG 2 cut(s) 94, 291
FaeI CATG 3 cut(s) 136, 295, 328
FaiI YATR 4 cut(s) 134, 293, 326, 349
FatI CATG 3 cut(s) 132, 291, 324
FbaI TGATCA 1 cut(s) 327
Fnu4HI GCNGC 3 cut(s) 15, 300, 339
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 3 cut(s) 15, 300, 339
FspBI CTAG 1 cut(s) 370
GluI GCNGC 3 cut(s) 15, 300, 339
HaeIII GGCC 3 cut(s) 122, 131, 214
HapII CCGG 3 cut(s) 192, 215, 270
Hin1II CATG 3 cut(s) 136, 295, 328
HinfI GANTC 1 cut(s) 55
HpaII CCGG 3 cut(s) 192, 215, 270
HphI GGTGA 1 cut(s) 206
Hpy188I TCNGA 2 cut(s) 114, 184
HpyAV CCTTC 1 cut(s) 165
HpyCH4III ACNGT 1 cut(s) 63
HpyCH4V TGCA 1 cut(s) 242
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpyF3I CTNAG 1 cut(s) 111
Hsp92II CATG 3 cut(s) 136, 295, 328
KroI GCCGGC 2 cut(s) 214, 269
KroNI GCCGGC 2 cut(s) 216, 271
Ksp22I TGATCA 1 cut(s) 327
Kzo9I GATC 5 cut(s) 79, 101, 179, 287, 327
LguI GCTCTTC 1 cut(s) 73
LmnI GCTCC 1 cut(s) 152
LpnPI CCDG 7 cut(s) 184, 205, 228, 228, 241, 279, 283
MaeI CTAG 1 cut(s) 370
MaeIII GTNAC 2 cut(s) 169, 194
MalI GATC 5 cut(s) 81, 103, 181, 289, 329
MboI GATC 5 cut(s) 79, 101, 179, 287, 327
MboII GAAGA 1 cut(s) 60
MflI RGATCY 1 cut(s) 287
MhlI GDGCHC 1 cut(s) 68
MlsI TGGCCA 1 cut(s) 122
MluNI TGGCCA 1 cut(s) 122
MnlI CCTC 4 cut(s) 120, 160, 178, 204
Mox20I TGGCCA 1 cut(s) 122
MroNI GCCGGC 2 cut(s) 214, 269
MscI TGGCCA 1 cut(s) 122
MslI CAYNNNNRTG 1 cut(s) 137
Msp20I TGGCCA 1 cut(s) 122
MspI CCGG 3 cut(s) 192, 215, 270
MwoI GCNNNNNNNGC 1 cut(s) 128
NaeI GCCGGC 2 cut(s) 216, 271
NcoI CCATGG 1 cut(s) 291
NdeII GATC 5 cut(s) 79, 101, 179, 287, 327
NgoMIV GCCGGC 2 cut(s) 214, 269
NlaIII CATG 3 cut(s) 136, 295, 328
NlaIV GGNNCC 2 cut(s) 148, 289
NmuCI GTSAC 2 cut(s) 169, 194
PaeR7I CTCGAG 1 cut(s) 208
PciSI GCTCTTC 1 cut(s) 73
PdiI GCCGGC 2 cut(s) 216, 271
PfeI GAWTC 1 cut(s) 55
PinAI ACCGGT 1 cut(s) 191
PkrI GCNGC 3 cut(s) 16, 301, 340
Ple19I CGATCG 1 cut(s) 82
PspN4I GGNNCC 2 cut(s) 148, 289
PspPI GGNCC 1 cut(s) 129
PspXI VCTCGAGB 1 cut(s) 208
PsuI RGATCY 1 cut(s) 287
PvuI CGATCG 1 cut(s) 82
RseI CAYNNNNRTG 1 cut(s) 137
SapI GCTCTTC 1 cut(s) 73
SatI GCNGC 3 cut(s) 15, 300, 339
Sau3AI GATC 5 cut(s) 79, 101, 179, 287, 327
Sau96I GGNCC 1 cut(s) 129
SduI GDGCHC 1 cut(s) 68
SetI ASST 2 cut(s) 247, 280
SfiI GGCCNNNNNGGCC 1 cut(s) 128
Sfr274I CTCGAG 1 cut(s) 208
SlaI CTCGAG 1 cut(s) 208
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 1 cut(s) 208
SmoI CTYRAG 1 cut(s) 208
SsiI CCGC 4 cut(s) 177, 285, 299, 338
SspMI CTAG 1 cut(s) 370
StyI CCWWGG 2 cut(s) 94, 291
TaaI ACNGT 1 cut(s) 63
TaqI TCGA 3 cut(s) 78, 104, 209
TauI GCSGC 2 cut(s) 302, 341
TfiI GAWTC 1 cut(s) 55
TscAI CASTG 1 cut(s) 174
TseFI GTSAC 2 cut(s) 169, 194
TseI GCWGC 1 cut(s) 14
Tsp45I GTSAC 2 cut(s) 169, 194
TspRI CASTG 1 cut(s) 174
XhoI CTCGAG 1 cut(s) 208
XspI CTAG 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.