RLG00000009075

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000981
Physical Location & Seq
Forward (+)
46112367 .. 46113891
1525 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009075

Sequence Viewer

Length: 465 bp
ATGAGACTTTGGGTACCCATATTTGGATGTCCAAATCAAATTGGGGAGGGAGGGAGGGAGGAAGAAGAAGGAAGAAGGAAGAAGACTAAGGAGGAGGAGAAGAAAATGAGACTTTGGGATAGCTATGCTGCTCTTTCTACTCATCTCCTCGACGGCAACGGCGATTGGATTCTACGGTGCTTTTCAAGTTTCGATCGGGTTCATCTCCTTGGGGGATCGAAGCCTCAGATGATGGCCATCTGGGCCGTGATGGTGTGTGGCTCCTCGCCTTCAATTTTGTCAGTGACAAGCGGATCAAAGGCGACCGGTGACTGGGTTTGCTCGAGGCCGGCTTTGATGAGCGACAATGGTTTTGCAGGTTTGGGTCTCTGGTGTTTCCCTTCTGGCAAAGGTGGTCGCGGATCCATGGCGGCGGCATTTTGTAGTGGTGTCTTACCCATGATCACAACGGCGGCAACACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

16.61

Weight (kDa)

8.59

Isoelectric Point (pI)

52.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 347
Acc65I GGTACC 1 cut(s) 13
AccB1I GGYRCC 1 cut(s) 13
AccII CGCG 1 cut(s) 399
AciI CCGC 5 cut(s) 291, 399, 410, 413, 452
AclWI GGATC 4 cut(s) 223, 301, 396, 409
AcoI YGGCCR 1 cut(s) 234
AfaI GTAC 1 cut(s) 15
AfiI CCNNNNNNNGG 2 cut(s) 23, 312
AgeI ACCGGT 1 cut(s) 305
AgsI TTSAA 2 cut(s) 186, 273
AluBI AGCT 1 cut(s) 123
AluI AGCT 1 cut(s) 123
Alw26I GTCTC 2 cut(s) 103, 371
AlwI GGATC 4 cut(s) 223, 301, 396, 409
Ama87I CYCGRG 1 cut(s) 322
AoxI GGCC 3 cut(s) 234, 243, 326
ApeKI GCWGC 1 cut(s) 128
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
AsiGI ACCGGT 1 cut(s) 305
Asp718I GGTACC 1 cut(s) 13
AspS9I GGNCC 1 cut(s) 243
AsuHPI GGTGA 1 cut(s) 320
AvaI CYCGRG 1 cut(s) 322
BalI TGGCCA 1 cut(s) 236
BamHI GGATCC 1 cut(s) 401
BanI GGYRCC 1 cut(s) 13
BbsI GAAGAC 1 cut(s) 89
BbvI GCAGC 1 cut(s) 115
BccI CCATC 3 cut(s) 226, 244, 245
BceAI ACGGC 3 cut(s) 169, 175, 230
BclI TGATCA 1 cut(s) 441
BcoDI GTCTC 2 cut(s) 103, 371
BfmI CTRYAG 1 cut(s) 461
BfuAI ACCTGC 1 cut(s) 347
BglI GCCNNNNNGGC 1 cut(s) 242
BisI GCNGC 4 cut(s) 129, 411, 414, 453
BlsI GCNGC 4 cut(s) 130, 412, 415, 454
BmeT110I CYCGRG 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 243
BmiI GGNNCC 3 cut(s) 15, 262, 403
BmrI ACTGGG 1 cut(s) 322
BmuI ACTGGG 1 cut(s) 322
BpiI GAAGAC 1 cut(s) 89
BsaBI GATNNNNATC 1 cut(s) 236
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 2 cut(s) 208, 405
BsaWI WCCGGW 1 cut(s) 305
Bsc4I CCNNNNNNNGG 2 cut(s) 23, 312
Bse118I RCCGGY 2 cut(s) 305, 328
Bse1I ACTGG 1 cut(s) 317
Bse8I GATNNNNATC 1 cut(s) 236
BseDI CCNNGG 2 cut(s) 208, 405
BseGI GGATG 1 cut(s) 32
BseJI GATNNNNATC 1 cut(s) 236
BseLI CCNNNNNNNGG 2 cut(s) 23, 312
BseMII CTCAG 1 cut(s) 239
BseNI ACTGG 1 cut(s) 317
BseRI GAGGAG 4 cut(s) 107, 110, 137, 253
BseXI GCAGC 1 cut(s) 115
Bsh1236I CGCG 1 cut(s) 399
Bsh1285I CGRYCG 2 cut(s) 196, 306
BshFI GGCC 3 cut(s) 236, 245, 328
BshNI GGYRCC 1 cut(s) 13
BshTI ACCGGT 1 cut(s) 305
BsiEI CGRYCG 2 cut(s) 196, 306
BsiHKCI CYCGRG 1 cut(s) 322
BsiSI CCGG 2 cut(s) 306, 329
BslI CCNNNNNNNGG 2 cut(s) 23, 312
BsmAI GTCTC 2 cut(s) 103, 371
BsnI GGCC 3 cut(s) 236, 245, 328
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 322
Bsp143I GATC 5 cut(s) 193, 215, 293, 401, 441
Bsp19I CCATGG 1 cut(s) 405
BspACI CCGC 5 cut(s) 291, 399, 410, 413, 452
BspANI GGCC 3 cut(s) 236, 245, 328
BspCNI CTCAG 1 cut(s) 238
BspFNI CGCG 1 cut(s) 399
BspLI GGNNCC 3 cut(s) 15, 262, 403
BspMI ACCTGC 1 cut(s) 347
BspPI GGATC 4 cut(s) 223, 301, 396, 409
BspT107I GGYRCC 1 cut(s) 13
BspTNI GGTCTC 1 cut(s) 371
BsrFI RCCGGY 2 cut(s) 305, 328
BsrI ACTGG 1 cut(s) 317
BssAI RCCGGY 2 cut(s) 305, 328
BssECI CCNNGG 2 cut(s) 208, 405
BssMI GATC 5 cut(s) 193, 215, 293, 401, 441
BssT1I CCWWGG 2 cut(s) 208, 405
Bst4CI ACNGT 1 cut(s) 177
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 2 cut(s) 87, 225
BstDSI CCRYGG 1 cut(s) 405
BstF5I GGATG 1 cut(s) 32
BstFNI CGCG 1 cut(s) 399
BstKTI GATC 5 cut(s) 196, 218, 296, 404, 444
BstMAI GTCTC 2 cut(s) 103, 371
BstMBI GATC 5 cut(s) 193, 215, 293, 401, 441
BstMCI CGRYCG 2 cut(s) 196, 306
BstMWI GCNNNNNNNGC 1 cut(s) 242
BstSFI CTRYAG 1 cut(s) 461
BstUI CGCG 1 cut(s) 399
BstV1I GCAGC 1 cut(s) 115
BstV2I GAAGAC 1 cut(s) 89
BstX2I RGATCY 1 cut(s) 401
BstYI RGATCY 1 cut(s) 401
BsuRI GGCC 3 cut(s) 236, 245, 328
BtgI CCRYGG 1 cut(s) 405
BtsCI GGATG 1 cut(s) 32
BtsIMutI CAGTG 1 cut(s) 288
BveI ACCTGC 1 cut(s) 347
Cac8I GCNNGC 1 cut(s) 330
Cfr10I RCCGGY 2 cut(s) 305, 328
Cfr13I GGNCC 1 cut(s) 243
Csp6I GTAC 1 cut(s) 14
CspAI ACCGGT 1 cut(s) 305
CviAII CATG 2 cut(s) 406, 439
CviJI RGCY 7 cut(s) 123, 223, 236, 245, 261, 328, 332
CviKI_1 RGCY 7 cut(s) 123, 223, 236, 245, 261, 328, 332
CviQI GTAC 1 cut(s) 14
DdeI CTNAG 2 cut(s) 87, 225
DpnI GATC 5 cut(s) 195, 217, 295, 403, 443
DpnII GATC 5 cut(s) 193, 215, 293, 401, 441
EaeI YGGCCR 1 cut(s) 234
Eco130I CCWWGG 2 cut(s) 208, 405
Eco31I GGTCTC 1 cut(s) 371
Eco88I CYCGRG 1 cut(s) 322
EcoT14I CCWWGG 2 cut(s) 208, 405
ErhI CCWWGG 2 cut(s) 208, 405
FaeI CATG 2 cut(s) 409, 442
FaiI YATR 5 cut(s) 20, 126, 407, 440, 463
FatI CATG 2 cut(s) 405, 438
FbaI TGATCA 1 cut(s) 441
Fnu4HI GCNGC 4 cut(s) 129, 411, 414, 453
FokI GGATG 1 cut(s) 39
Fsp4HI GCNGC 4 cut(s) 129, 411, 414, 453
GluI GCNGC 4 cut(s) 129, 411, 414, 453
HaeIII GGCC 3 cut(s) 236, 245, 328
HapII CCGG 2 cut(s) 306, 329
Hin1II CATG 2 cut(s) 409, 442
HinfI GANTC 1 cut(s) 169
HpaII CCGG 2 cut(s) 306, 329
HphI GGTGA 1 cut(s) 320
Hpy188I TCNGA 1 cut(s) 228
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 4 cut(s) 62, 69, 279, 390
HpyCH4III ACNGT 1 cut(s) 177
HpyCH4V TGCA 1 cut(s) 356
HpyF10VI GCNNNNNNNGC 1 cut(s) 242
HpyF3I CTNAG 2 cut(s) 87, 225
Hsp92II CATG 2 cut(s) 409, 442
KpnI GGTACC 1 cut(s) 17
KroI GCCGGC 1 cut(s) 328
KroNI GCCGGC 1 cut(s) 330
Ksp22I TGATCA 1 cut(s) 441
Kzo9I GATC 5 cut(s) 193, 215, 293, 401, 441
LmnI GCTCC 1 cut(s) 266
LpnPI CCDG 7 cut(s) 226, 298, 319, 342, 342, 355, 369
Lsp1109I GCAGC 1 cut(s) 115
MaeIII GTNAC 2 cut(s) 283, 308
MalI GATC 5 cut(s) 195, 217, 295, 403, 443
MboI GATC 5 cut(s) 193, 215, 293, 401, 441
MboII GAAGA 6 cut(s) 74, 77, 84, 91, 94, 112
MflI RGATCY 1 cut(s) 401
MlsI TGGCCA 1 cut(s) 236
MluCI AATT 2 cut(s) 39, 273
MluNI TGGCCA 1 cut(s) 236
Mox20I TGGCCA 1 cut(s) 236
MroNI GCCGGC 1 cut(s) 328
MscI TGGCCA 1 cut(s) 236
Msp20I TGGCCA 1 cut(s) 236
MspI CCGG 2 cut(s) 306, 329
MvnI CGCG 1 cut(s) 399
MwoI GCNNNNNNNGC 1 cut(s) 242
NaeI GCCGGC 1 cut(s) 330
NcoI CCATGG 1 cut(s) 405
NdeII GATC 5 cut(s) 193, 215, 293, 401, 441
NgoMIV GCCGGC 1 cut(s) 328
NlaIII CATG 2 cut(s) 409, 442
NlaIV GGNNCC 3 cut(s) 15, 262, 403
NmuCI GTSAC 2 cut(s) 283, 308
PaeR7I CTCGAG 1 cut(s) 322
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PdiI GCCGGC 1 cut(s) 330
PfeI GAWTC 1 cut(s) 169
PinAI ACCGGT 1 cut(s) 305
PkrI GCNGC 4 cut(s) 130, 412, 415, 454
Ple19I CGATCG 1 cut(s) 196
PspN4I GGNNCC 3 cut(s) 15, 262, 403
PspPI GGNCC 1 cut(s) 243
PspXI VCTCGAGB 1 cut(s) 322
PsuI RGATCY 1 cut(s) 401
PvuI CGATCG 1 cut(s) 196
RsaI GTAC 1 cut(s) 15
RsaNI GTAC 1 cut(s) 14
SatI GCNGC 4 cut(s) 129, 411, 414, 453
Sau3AI GATC 5 cut(s) 193, 215, 293, 401, 441
Sau96I GGNCC 1 cut(s) 243
SetI ASST 3 cut(s) 125, 361, 394
SfcI CTRYAG 1 cut(s) 461
SfiI GGCCNNNNNGGCC 1 cut(s) 242
Sfr274I CTCGAG 1 cut(s) 322
SlaI CTCGAG 1 cut(s) 322
SmlI CTYRAG 1 cut(s) 322
SmoI CTYRAG 1 cut(s) 322
Sse9I AATT 2 cut(s) 39, 273
SsiI CCGC 5 cut(s) 291, 399, 410, 413, 452
StyI CCWWGG 2 cut(s) 208, 405
TaaI ACNGT 1 cut(s) 177
TaqI TCGA 4 cut(s) 150, 192, 218, 323
TasI AATT 2 cut(s) 39, 273
TauI GCSGC 3 cut(s) 413, 416, 455
TfiI GAWTC 1 cut(s) 169
TscAI CASTG 1 cut(s) 288
TseFI GTSAC 2 cut(s) 283, 308
TseI GCWGC 1 cut(s) 128
Tsp45I GTSAC 2 cut(s) 283, 308
TspDTI ATGAA 1 cut(s) 191
TspRI CASTG 1 cut(s) 288
XhoI CTCGAG 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.